Click here to switch to the map view.

The map label for this gene is mdh [H]

Identifier: 145294834

GI number: 145294834

Start: 863650

End: 864693

Strand: Reverse

Name: mdh [H]

Synonym: cgR_0781

Alternate gene names: 145294834

Gene position: 864693-863650 (Counterclockwise)

Preceding gene: 145294835

Following gene: 145294833

Centisome position: 26.09

GC content: 52.78

Gene sequence:

>1044_bases
ATGCCAGAAGTCACTGTCAACGCCCAACAACTCACTGTTCTCTGCACAGACATCCTCACCAAAACTGGAGTACCTGCAGC
AGACGCCCATCTTGTCGCTGATAGTTTGGTGCAGGCAGATCTTTGGGGTCATCCCTCCCACGGTGTGCTTCGACTGCCTT
GGTATGTGCGCAGACTCCACAGTGGCGCAATGACTACACATGCACACGTGGAGGTTCTCAATGATTTGGGTGCCGTGTTT
GCGTTGGATGGACACAATGGAATCGGCCAAATTTTAGCTGATTATGCTCGTAAAGAAGCAGTGACTAGGGCAATGATGTT
CGGCATCGGTGCGGTGTCGGTGCGCAACTCCAATCATTTTGGAACTGCCATGTACTACACCCGGAAAGCGGCAGCGCAAG
GATGTGTTTCCATTCTCACCACCAATGCATCTCCGGCGATGGCGCCCTGGGGTGGCAGAGAAAAACGGATCGGTACCAAC
CCATGGTCTATCGCGGCACCTCTTAGAGAAACGGCTGCAGTAGTCGATATAGCCAATACTGCGGTTGCGCGCGGAAAGAT
CTACCACGCACGACAGACAAACATGCCCATTCCTGAGACTTGGGCGATCACGAGTGAGGGCGCACCCACCACGGATCCTG
CTGAGGCAATCAACGGTGTCGTCCTTCCCATGGCTGGTCACAAAGGTTATGCGATTAGCTTCATGATGGATGTGCTTTCT
GGAGTTCTCACTGGTTCCCAGCACAGCACAAAGGTACATGGTCCGTATGATCCCACTCCCCCAGGTGGAGCTGGCCACTT
GTTCATCGCGTTGGATGTTGCAGCGTTTCGCGATCCACAAGATTTCGATGACGCACTCAGCGATCTGGTTGGGGAAGTTA
AATCCACTCCGAAAGCACAAAACACCGAGGAGATTTTCTACCCCGGTGAATTGGAAGACCGTGCGCATCGGAAAAACTCT
GCGCACGGTATTTCATTGCCTGAAAAAACGTGGATGGAACTGCAAGAACTGGCAATCGAGAACCATGTTGTCACTCACCG
TTGA

Upstream 100 bases:

>100_bases
GGTCTTAGAACCAGCGTGCACTGATGGCGATTAAAGGGGGTTGCGCCTATACCTATTACTAGTATACATTTCGGTATACC
TAAATCGAAATGAGGGATTT

Downstream 100 bases:

>100_bases
TCTGCGCGTTAAACCTGGCCATCTGGAAGAATTCCTCAACGCCATTTCGGAGAATGCGCAGCGAAGCTTTCAGGATGAAG
AAGGTTGTGTGTATTTCGAC

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MPEVTVNAQQLTVLCTDILTKTGVPAADAHLVADSLVQADLWGHPSHGVLRLPWYVRRLHSGAMTTHAHVEVLNDLGAVF
ALDGHNGIGQILADYARKEAVTRAMMFGIGAVSVRNSNHFGTAMYYTRKAAAQGCVSILTTNASPAMAPWGGREKRIGTN
PWSIAAPLRETAAVVDIANTAVARGKIYHARQTNMPIPETWAITSEGAPTTDPAEAINGVVLPMAGHKGYAISFMMDVLS
GVLTGSQHSTKVHGPYDPTPPGGAGHLFIALDVAAFRDPQDFDDALSDLVGEVKSTPKAQNTEEIFYPGELEDRAHRKNS
AHGISLPEKTWMELQELAIENHVVTHR

Sequences:

>Translated_347_residues
MPEVTVNAQQLTVLCTDILTKTGVPAADAHLVADSLVQADLWGHPSHGVLRLPWYVRRLHSGAMTTHAHVEVLNDLGAVF
ALDGHNGIGQILADYARKEAVTRAMMFGIGAVSVRNSNHFGTAMYYTRKAAAQGCVSILTTNASPAMAPWGGREKRIGTN
PWSIAAPLRETAAVVDIANTAVARGKIYHARQTNMPIPETWAITSEGAPTTDPAEAINGVVLPMAGHKGYAISFMMDVLS
GVLTGSQHSTKVHGPYDPTPPGGAGHLFIALDVAAFRDPQDFDDALSDLVGEVKSTPKAQNTEEIFYPGELEDRAHRKNS
AHGISLPEKTWMELQELAIENHVVTHR
>Mature_346_residues
PEVTVNAQQLTVLCTDILTKTGVPAADAHLVADSLVQADLWGHPSHGVLRLPWYVRRLHSGAMTTHAHVEVLNDLGAVFA
LDGHNGIGQILADYARKEAVTRAMMFGIGAVSVRNSNHFGTAMYYTRKAAAQGCVSILTTNASPAMAPWGGREKRIGTNP
WSIAAPLRETAAVVDIANTAVARGKIYHARQTNMPIPETWAITSEGAPTTDPAEAINGVVLPMAGHKGYAISFMMDVLSG
VLTGSQHSTKVHGPYDPTPPGGAGHLFIALDVAAFRDPQDFDDALSDLVGEVKSTPKAQNTEEIFYPGELEDRAHRKNSA
HGISLPEKTWMELQELAIENHVVTHR

Specific function: Acts on oxaloacetate, sulfopyruvate but not on pyruvate. Has a higher selectivity for the coenzyme NADH than for NADPH [H]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786727, Length=341, Percent_Identity=32.8445747800587, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787020, Length=340, Percent_Identity=32.3529411764706, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1790000, Length=335, Percent_Identity=28.3582089552239, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17507179, Length=347, Percent_Identity=31.700288184438, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17536633, Length=322, Percent_Identity=29.8136645962733, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24667908, Length=337, Percent_Identity=32.9376854599407, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI45553211, Length=337, Percent_Identity=32.9376854599407, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24667912, Length=337, Percent_Identity=32.9376854599407, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24667904, Length=337, Percent_Identity=32.9376854599407, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24658010, Length=325, Percent_Identity=29.2307692307692, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24648360, Length=334, Percent_Identity=28.1437125748503, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.272; =1.1.1.37; =1.1.1.82 [H]

Molecular weight: Translated: 37284; Mature: 37152

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVTVNAQQLTVLCTDILTKTGVPAADAHLVADSLVQADLWGHPSHGVLRLPWYVRRLH
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHH
SGAMTTHAHVEVLNDLGAVFALDGHNGIGQILADYARKEAVTRAMMFGIGAVSVRNSNHF
CCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
GTAMYYTRKAAAQGCVSILTTNASPAMAPWGGREKRIGTNPWSIAAPLRETAAVVDIANT
CHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
AVARGKIYHARQTNMPIPETWAITSEGAPTTDPAEAINGVVLPMAGHKGYAISFMMDVLS
HHHCCEEEEECCCCCCCCCCEEEECCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHH
GVLTGSQHSTKVHGPYDPTPPGGAGHLFIALDVAAFRDPQDFDDALSDLVGEVKSTPKAQ
HHHCCCCCCCEECCCCCCCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCC
NTEEIFYPGELEDRAHRKNSAHGISLPEKTWMELQELAIENHVVTHR
CCCEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PEVTVNAQQLTVLCTDILTKTGVPAADAHLVADSLVQADLWGHPSHGVLRLPWYVRRLH
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHH
SGAMTTHAHVEVLNDLGAVFALDGHNGIGQILADYARKEAVTRAMMFGIGAVSVRNSNHF
CCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
GTAMYYTRKAAAQGCVSILTTNASPAMAPWGGREKRIGTNPWSIAAPLRETAAVVDIANT
CHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
AVARGKIYHARQTNMPIPETWAITSEGAPTTDPAEAINGVVLPMAGHKGYAISFMMDVLS
HHHCCEEEEECCCCCCCCCCEEEECCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHH
GVLTGSQHSTKVHGPYDPTPPGGAGHLFIALDVAAFRDPQDFDDALSDLVGEVKSTPKAQ
HHHCCCCCCCEECCCCCCCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCC
NTEEIFYPGELEDRAHRKNSAHGISLPEKTWMELQELAIENHVVTHR
CCCEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2110059; 10850983 [H]