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The map label for this gene is metZ [H]

Identifier: 145294828

GI number: 145294828

Start: 855028

End: 856341

Strand: Reverse

Name: metZ [H]

Synonym: cgR_0775

Alternate gene names: 145294828

Gene position: 856341-855028 (Counterclockwise)

Preceding gene: 145294833

Following gene: 145294827

Centisome position: 25.84

GC content: 57.76

Gene sequence:

>1314_bases
ATGCCAAAGTACGACAATTCCAATGCTGACCAGTGGGGCTTTGAAACCCGCTCCATTCACGCAGGCCAGTCAGTAGACGC
ACAGACCAGCGCACGAAACCTTCCGATCTACCAATCCACCGCTTTCGTGTTCGACTCCGCTGAGCACGCCAAGCAGCGTT
TCGCACTTGAGGATCTGGGCCCTGTTTACTCCCGCCTCACCAACCCAACCGTTGAGGCTTTGGAAAACCGCATCGCTTCC
CTCGAAGGTGGCGTCCACGCTGTAGCGTTCTCCTCCGGACAGGCCGCAACCACCAACGCCATTTTGAACCTGGCAGGAGC
AGGCGACCACATCGTCACCTCCCCACGCCTCTACGGTGGCACCGAGACTCTATTCCTTATCACTCTTAACCGCTTGGGTA
TCGATGTTTCCTTCGTGGAAAACCCCGACGACCCTGAGTCCTGGCAGGCAGCCGTTCAGCCAAACACCAAGGCATTCTTC
GGCGAGACTTTCGCCAACCCACAGGCAGACGTCCTGGATATTCCTGCCGTGGCTGAAGTTGCGCACCGCAACAGCGTTCC
ACTGATCATCGACAACACCATCGCTACCGCAGCGCTCGTGCGCCCGCTCGAGCTCGGCGCAGACGTTGTCGTCGCTTCCC
TCACCAAGTTCTACACCGGCAACGGCTCCGGACTGGGCGGCGTGCTTATCGACGGCGGAAAGTTCGATTGGACTGTCGAA
AAGGATGGAAAGCCAGTATTCCCCTACTTCGTCACTCCAGATGCTGCTTACCACGGATTGAAGTACGCAGACCTTGGTGC
ACCAGCCTTCGGCCTCAAGGTTCGCGTTGGTCTTCTACGCGACACCGGCTCCACCCTCTCCGCATTCAACGCATGGGCTG
CAGTCCAGGGCATCGACACCCTTTCCCTGCGTCTGGAGCGCCACAACGAAAACGCCATCAAGGTTGCAGAATTCCTCAAC
AACCACGAGAAGGTGGAAAAGGTTAACTTCGCAGGCCTGAAGGATTCCCCTTGGTACGCAACCAAGGAAAAGCTTGGCCT
GAAGTACACCGGCTCCGTTCTCACCTTCGAGATCAAGGGCGGCAAGGATGAGGCTTGGGCATTTATCGACGCCCTGAAGC
TGCACTCCAACCTTGCAAACATCGGCGATGTTCGCTCCCTCGTTGTCCACCCAGCAACCACCACTCACTCACAGTCCGAC
GAAGCTGGCCTAGCACGCGCGGGCGTTACCCAGTCCACCGTCCGCCTGTCCGTTGGCATCGAAACCATTGATGACATCAT
CGCTGACCTCGAAGGCGGATTTGCAGCAATCTAG

Upstream 100 bases:

>100_bases
AGAATAAATTTATACCGCACAGTCTATTGCAATAGACCAAGCTGTTCAGTAGGGTGCATGGGAGAAGAATTTCCTAATAA
AAACTCTTAAGGACCTCCAA

Downstream 100 bases:

>100_bases
CTTTAACTAGACTCACCCTAGTGCTTAAAGCGCTGGGGTTTTTCTTTTTCAAACTCGCGAGAATGCAAACTAGACTAGAC
AGAGCTGTCCATATACACTG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: L-methionine; acetate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPKYDNSNADQWGFETRSIHAGQSVDAQTSARNLPIYQSTAFVFDSAEHAKQRFALEDLGPVYSRLTNPTVEALENRIAS
LEGGVHAVAFSSGQAATTNAILNLAGAGDHIVTSPRLYGGTETLFLITLNRLGIDVSFVENPDDPESWQAAVQPNTKAFF
GETFANPQADVLDIPAVAEVAHRNSVPLIIDNTIATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGKFDWTVE
KDGKPVFPYFVTPDAAYHGLKYADLGAPAFGLKVRVGLLRDTGSTLSAFNAWAAVQGIDTLSLRLERHNENAIKVAEFLN
NHEKVEKVNFAGLKDSPWYATKEKLGLKYTGSVLTFEIKGGKDEAWAFIDALKLHSNLANIGDVRSLVVHPATTTHSQSD
EAGLARAGVTQSTVRLSVGIETIDDIIADLEGGFAAI

Sequences:

>Translated_437_residues
MPKYDNSNADQWGFETRSIHAGQSVDAQTSARNLPIYQSTAFVFDSAEHAKQRFALEDLGPVYSRLTNPTVEALENRIAS
LEGGVHAVAFSSGQAATTNAILNLAGAGDHIVTSPRLYGGTETLFLITLNRLGIDVSFVENPDDPESWQAAVQPNTKAFF
GETFANPQADVLDIPAVAEVAHRNSVPLIIDNTIATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGKFDWTVE
KDGKPVFPYFVTPDAAYHGLKYADLGAPAFGLKVRVGLLRDTGSTLSAFNAWAAVQGIDTLSLRLERHNENAIKVAEFLN
NHEKVEKVNFAGLKDSPWYATKEKLGLKYTGSVLTFEIKGGKDEAWAFIDALKLHSNLANIGDVRSLVVHPATTTHSQSD
EAGLARAGVTQSTVRLSVGIETIDDIIADLEGGFAAI
>Mature_436_residues
PKYDNSNADQWGFETRSIHAGQSVDAQTSARNLPIYQSTAFVFDSAEHAKQRFALEDLGPVYSRLTNPTVEALENRIASL
EGGVHAVAFSSGQAATTNAILNLAGAGDHIVTSPRLYGGTETLFLITLNRLGIDVSFVENPDDPESWQAAVQPNTKAFFG
ETFANPQADVLDIPAVAEVAHRNSVPLIIDNTIATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGKFDWTVEK
DGKPVFPYFVTPDAAYHGLKYADLGAPAFGLKVRVGLLRDTGSTLSAFNAWAAVQGIDTLSLRLERHNENAIKVAEFLNN
HEKVEKVNFAGLKDSPWYATKEKLGLKYTGSVLTFEIKGGKDEAWAFIDALKLHSNLANIGDVRSLVVHPATTTHSQSDE
AGLARAGVTQSTVRLSVGIETIDDIIADLEGGFAAI

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=408, Percent_Identity=31.6176470588235, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI299473759, Length=408, Percent_Identity=27.6960784313726, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI25453487, Length=407, Percent_Identity=28.5012285012285, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1790375, Length=423, Percent_Identity=28.6052009456265, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1789383, Length=440, Percent_Identity=27.9545454545455, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=402, Percent_Identity=29.8507462686567, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=412, Percent_Identity=31.3106796116505, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17538055, Length=413, Percent_Identity=31.2348668280872, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=42.2374429223744, Blast_Score=295, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6319307, Length=431, Percent_Identity=33.1786542923434, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=441, Percent_Identity=27.891156462585, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=337, Percent_Identity=26.1127596439169, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=411, Percent_Identity=24.8175182481752, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=35.9338061465721, Blast_Score=216, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.49

Molecular weight: Translated: 46753; Mature: 46622

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYDNSNADQWGFETRSIHAGQSVDAQTSARNLPIYQSTAFVFDSAEHAKQRFALEDLG
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEECEEEEECCHHHHHHHHHHHHHH
PVYSRLTNPTVEALENRIASLEGGVHAVAFSSGQAATTNAILNLAGAGDHIVTSPRLYGG
HHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCEECEEEEEECCCCCCEEECCEECCC
TETLFLITLNRLGIDVSFVENPDDPESWQAAVQPNTKAFFGETFANPQADVLDIPAVAEV
CCEEEEEEEEHHCEEEEECCCCCCHHHHHEECCCCCCEECCHHCCCCCCCEEECCHHHHH
AHRNSVPLIIDNTIATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGKFDWTVE
HHCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEE
KDGKPVFPYFVTPDAAYHGLKYADLGAPAFGLKVRVGLLRDTGSTLSAFNAWAAVQGIDT
CCCCEECCEEECCCHHHCCCEEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCE
LSLRLERHNENAIKVAEFLNNHEKVEKVNFAGLKDSPWYATKEKLGLKYTGSVLTFEIKG
EEEEEEECCCCHHHHHHHHCCHHHHHHEEECCCCCCCCCCHHHHCCEEEECCEEEEEEEC
GKDEAWAFIDALKLHSNLANIGDVRSLVVHPATTTHSQSDEAGLARAGVTQSTVRLSVGI
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCCCCCEEEEEEECH
ETIDDIIADLEGGFAAI
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PKYDNSNADQWGFETRSIHAGQSVDAQTSARNLPIYQSTAFVFDSAEHAKQRFALEDLG
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEECEEEEECCHHHHHHHHHHHHHH
PVYSRLTNPTVEALENRIASLEGGVHAVAFSSGQAATTNAILNLAGAGDHIVTSPRLYGG
HHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCEECEEEEEECCCCCCEEECCEECCC
TETLFLITLNRLGIDVSFVENPDDPESWQAAVQPNTKAFFGETFANPQADVLDIPAVAEV
CCEEEEEEEEHHCEEEEECCCCCCHHHHHEECCCCCCEECCHHCCCCCCCEEECCHHHHH
AHRNSVPLIIDNTIATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGKFDWTVE
HHCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEE
KDGKPVFPYFVTPDAAYHGLKYADLGAPAFGLKVRVGLLRDTGSTLSAFNAWAAVQGIDT
CCCCEECCEEECCCHHHCCCEEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCE
LSLRLERHNENAIKVAEFLNNHEKVEKVNFAGLKDSPWYATKEKLGLKYTGSVLTFEIKG
EEEEEEECCCCHHHHHHHHCCHHHHHHEEECCCCCCCCCCHHHHCCEEEECCEEEEEEEC
GKDEAWAFIDALKLHSNLANIGDVRSLVVHPATTTHSQSDEAGLARAGVTQSTVRLSVGI
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCCCCCEEEEEEECH
ETIDDIIADLEGGFAAI
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: O-acetyl-L-homoserine; methanethiol

Specific reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]