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The map label for this gene is ppsA [H]

Identifier: 145294717

GI number: 145294717

Start: 723047

End: 724141

Strand: Reverse

Name: ppsA [H]

Synonym: cgR_0665

Alternate gene names: 145294717

Gene position: 724141-723047 (Counterclockwise)

Preceding gene: 145294718

Following gene: 145294716

Centisome position: 21.85

GC content: 51.42

Gene sequence:

>1095_bases
ATGACCAACAGTTTGAACATCCCGTTTGTCCAGCGCTTCGATGAAGGCCTGGATCCTGTTCTAGAAGTACTCGGTGGCAA
GGGCGCTTCACTAGTCACCATGACAGATGCTGGAATGCCCGTTCCACCTGGATTTGTGGTCACTACTGCCAGCTTTGATG
AATTCATCCGTGAAGCAGGGGTTGCTGAACACATCGATAAATTCCTAAACGATCTCGATGCAGAAGATGTTAAGGAAGTG
GATCGAGTTTCTGCGATCATCCGCGATGAACTGTGCAGTCTTGAAGTTCCAGAGAATGCTCGTTTCGCAGTGCACCAGGC
TTATCGCGATCTCATGGAACGCTGTGGTGGCGACGTCCCGGTTGCTGTCCGGTCGTCGGCCACTGCCGAAGATTTACCCG
ATGCTTCCTTCGCAGGCCAACAAGACACTTACTTATGGCAGGTCGGGCTGAGTGCAGTCACTGAACATATTCGCAAATGC
TGGGCTTCGCTATTTACTTCCCGTGCCATTATTTACCGTCTGAAAAACAACATCCCCAATGAGGGCCTCTCCATGGCTGT
AGTTGTTCAAAAGATGGTCAACTCTCGTGTCGCAGGCGTGGCTATCACTATGAATCCTTCCAACGGCGACCGCTCGAAAA
TTACCATCGATTCCTCATGGGGTGTTGGTGAAATGGTGGTCTCAGGTGAAGTGACACCAGACAATATCTTGCTGGACAAG
ATCACGCTGCAGGTTGTCTCCGAACACATTGGAAGTAAGCACGCTGAACTCATCCCCGATGCCACCAGTGGAAGCCTCGT
GGAAAAGCCCGTTGATGAAGAACGCGCAAACCGCCGCAGTCTGACTGATGAGGAAATGTTCGCTGTGGCGCAAATGGCTA
AGCGTGCAGAAAAACACTACAAGTGCCCTCAAGACATCGAATGGGCATTGGACGCTGACCTGCCAGATGGAGAAAACCTT
CTGTTATTGCAATCCCGCCCGGAAACTATCCACTCCAACGGTGTGAAGAAGGAAACCCCAACTCCGCAGGCTGCCAAAAC
CATAGGCACCTTCGATTTCAGCTCAATCACCGTCGCAATGACCGGCACGAAGTAA

Upstream 100 bases:

>100_bases
GGACGAAGCTATCGGGTTCCGGGAGAACCTCTCCTTCCGCGTCCCCACTTCTGTTCCCGTGACTTGGAACGCTTAACGCT
TTATTAAATAAGGAGACACC

Downstream 100 bases:

>100_bases
AACCACCGCATCTTTTCGTCGAAAAGCATCTAAAAGGAGTTTGACCATGGCTAATAAATCTTTCCCCAAGCCCTCCGATC
TTCCAGTGCCCAGGGGCGCG

Product: hypothetical protein

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTNSLNIPFVQRFDEGLDPVLEVLGGKGASLVTMTDAGMPVPPGFVVTTASFDEFIREAGVAEHIDKFLNDLDAEDVKEV
DRVSAIIRDELCSLEVPENARFAVHQAYRDLMERCGGDVPVAVRSSATAEDLPDASFAGQQDTYLWQVGLSAVTEHIRKC
WASLFTSRAIIYRLKNNIPNEGLSMAVVVQKMVNSRVAGVAITMNPSNGDRSKITIDSSWGVGEMVVSGEVTPDNILLDK
ITLQVVSEHIGSKHAELIPDATSGSLVEKPVDEERANRRSLTDEEMFAVAQMAKRAEKHYKCPQDIEWALDADLPDGENL
LLLQSRPETIHSNGVKKETPTPQAAKTIGTFDFSSITVAMTGTK

Sequences:

>Translated_364_residues
MTNSLNIPFVQRFDEGLDPVLEVLGGKGASLVTMTDAGMPVPPGFVVTTASFDEFIREAGVAEHIDKFLNDLDAEDVKEV
DRVSAIIRDELCSLEVPENARFAVHQAYRDLMERCGGDVPVAVRSSATAEDLPDASFAGQQDTYLWQVGLSAVTEHIRKC
WASLFTSRAIIYRLKNNIPNEGLSMAVVVQKMVNSRVAGVAITMNPSNGDRSKITIDSSWGVGEMVVSGEVTPDNILLDK
ITLQVVSEHIGSKHAELIPDATSGSLVEKPVDEERANRRSLTDEEMFAVAQMAKRAEKHYKCPQDIEWALDADLPDGENL
LLLQSRPETIHSNGVKKETPTPQAAKTIGTFDFSSITVAMTGTK
>Mature_363_residues
TNSLNIPFVQRFDEGLDPVLEVLGGKGASLVTMTDAGMPVPPGFVVTTASFDEFIREAGVAEHIDKFLNDLDAEDVKEVD
RVSAIIRDELCSLEVPENARFAVHQAYRDLMERCGGDVPVAVRSSATAEDLPDASFAGQQDTYLWQVGLSAVTEHIRKCW
ASLFTSRAIIYRLKNNIPNEGLSMAVVVQKMVNSRVAGVAITMNPSNGDRSKITIDSSWGVGEMVVSGEVTPDNILLDKI
TLQVVSEHIGSKHAELIPDATSGSLVEKPVDEERANRRSLTDEEMFAVAQMAKRAEKHYKCPQDIEWALDADLPDGENLL
LLQSRPETIHSNGVKKETPTPQAAKTIGTFDFSSITVAMTGTK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=363, Percent_Identity=36.3636363636364, Blast_Score=197, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17564524, Length=321, Percent_Identity=32.7102803738318, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 39701; Mature: 39570

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSLNIPFVQRFDEGLDPVLEVLGGKGASLVTMTDAGMPVPPGFVVTTASFDEFIREAG
CCCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHCC
VAEHIDKFLNDLDAEDVKEVDRVSAIIRDELCSLEVPENARFAVHQAYRDLMERCGGDVP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
VAVRSSATAEDLPDASFAGQQDTYLWQVGLSAVTEHIRKCWASLFTSRAIIYRLKNNIPN
EEEECCCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EGLSMAVVVQKMVNSRVAGVAITMNPSNGDRSKITIDSSWGVGEMVVSGEVTPDNILLDK
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHH
ITLQVVSEHIGSKHAELIPDATSGSLVEKPVDEERANRRSLTDEEMFAVAQMAKRAEKHY
HHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KCPQDIEWALDADLPDGENLLLLQSRPETIHSNGVKKETPTPQAAKTIGTFDFSSITVAM
CCCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEEE
TGTK
ECCC
>Mature Secondary Structure 
TNSLNIPFVQRFDEGLDPVLEVLGGKGASLVTMTDAGMPVPPGFVVTTASFDEFIREAG
CCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHCC
VAEHIDKFLNDLDAEDVKEVDRVSAIIRDELCSLEVPENARFAVHQAYRDLMERCGGDVP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
VAVRSSATAEDLPDASFAGQQDTYLWQVGLSAVTEHIRKCWASLFTSRAIIYRLKNNIPN
EEEECCCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EGLSMAVVVQKMVNSRVAGVAITMNPSNGDRSKITIDSSWGVGEMVVSGEVTPDNILLDK
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHH
ITLQVVSEHIGSKHAELIPDATSGSLVEKPVDEERANRRSLTDEEMFAVAQMAKRAEKHY
HHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KCPQDIEWALDADLPDGENLLLLQSRPETIHSNGVKKETPTPQAAKTIGTFDFSSITVAM
CCCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEEE
TGTK
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]