Click here to switch to the map view.

The map label for this gene is yvkC [H]

Identifier: 145294716

GI number: 145294716

Start: 721099

End: 723000

Strand: Reverse

Name: yvkC [H]

Synonym: cgR_0664

Alternate gene names: 145294716

Gene position: 723000-721099 (Counterclockwise)

Preceding gene: 145294717

Following gene: 145294715

Centisome position: 21.82

GC content: 51.95

Gene sequence:

>1902_bases
ATGGCTAATAAATCTTTCCCCAAGCCCTCCGATCTTCCAGTGCCCAGGGGCGCGGAAGGTTGGGAAGATCTGTACCCGTA
CTACCTGGTTTTCCAAGACAAGCTCATGGATGAAGAAAACGAGAAATTCTGGTTCTGCGATTCACAGCACTGGCCAACTG
TGTTCAAGCCTTTTGAAACTATCGGTGGTGAATTCGCTGTAAAGTGCCTCGGCCAATACAACGCTCGGCATTTGATGATC
CCGAATGCCAATGGCATCGAGTTCCGCGTGCATCTGGGATACCTCTATATGTCCCCTATTCCAGTGCCTGAAGATCAGAT
TGCGGAACGCGTCCCCATGTTCCAGGAACGCATCACGCACTACTTCCAAAACTGGGAGCCAATGCTGGCAAATTGGAAGG
AGCGAGTATTAGGAACCATCAATGAGCTGGAATCTCTAGAATTCAAGCCACTGCCTGACTACGTGCCTATCTATGATATT
GTCTCCGGAAAAGCCAAAGACGGCACCGAAGTACTCATGGAAAACTTCGATCGGCTCATTCAGCTCGCCTACCAAAACTG
GCAATACCACTTTGAGTTCCTCAACTTGGGTTACATCGCTTACCTAGATTTCTTCAATTTCTGCAAGGAAGTCTTCCCAG
ATATCCCTGATCAATCAATTTCGATGATGGTTCAGGGCGTGGATATGGAGCTGTTCCGCCCCGATGATGAACTAAAGATT
CTGGCACAACTAGCGGTCGACCTTGGCCTGCAAACTCACTTTGCCAACCCGGATGATCCTCAAGCTACCTTGGCTGCTAT
CGCAAAGGCAGAAGGCGGCGCGACATGGATAGCGCGCTGGGAAGAAGCACAAGATCCGTGGTTCAACTTCACCGTCGGTA
ATGGCTTCTACGGTCACGATAAATACTGGATCGAGCACCTGGAACTTCCACTGGGGTACATCGCGGATTACATCCGCCGC
CTAGATGAAGGCCAAACCATCTCCCGCCCGAAAGATGAACTCATCGCAGAAAAGGATCGCGTGGTGGAAGAATACCGCGA
CCTTTTGGATGGAGAACAGCTCGCGCAGTTTGATGCAAAATGCGGCCTCGCGGCTACTGCATACCCTTATGTGGAAAACC
ATAACTTCTACATCGAGCACTGGACTATGTCAGTGTTTTGGCGCAAAGTACGCGAACTTTCCCGTACTCTCCAGGGCTAC
GGTTTCTGGGAGAACGAGGAAGACCTGCTGTATCTCAACCGCACGGAAGTCCGCGATGTCCTCTTCGACCTGGCTACAGC
GTGGGGTGTCGGTGCACCTGGTGGTCCAATTGGCACGATCATTTGGCCAGAAGAAATTGAGCGCAGAAAAGCAATTGTCA
CCGCTTTGAAAACTGCCCGACCAGCACCAGCTCTAAACACTCCTCCAGAGTCCATCACCGAACCTTTCACCCGCATGCTC
TGGGGAATCACCACCGAACAGGTGCAATCATGGTTGGGCAATGATGAGGATACCGAAGAAGGAACCCTTAAAGGCATGGC
TGCATCCCCTGGTGTGGTGGAAGGTTACGCCCGAGTAATTCTCAGCGCAGATGACCTTTCAGAAATCCAGCAGGATGAAA
TCCTCGTTGCCCCTGTAACAGCACCTTCTTGGGGCCCAATCTTTGGCAAAATCAAGGCAACAGTCACTGATATTGGTGGC
ATGATGAGCCATGCTGCGATCGTGTGCCGCGAATACGGCTTGCCGGCTGTTACTGGAACTGGCGCTGCATCCACCACCAT
CAAAACCGGCGATTACCTCAAGGTCGATGGAACCAAGGGCAAGGTTGTCATTGTTGATCCAGATGCGCCACGCATCGAAG
GACCCGGCGCGCACAGCCATGCGCACGAAGTAGCAGCACATGGGGTGGATACACATGCCTAG

Upstream 100 bases:

>100_bases
ATAGGCACCTTCGATTTCAGCTCAATCACCGTCGCAATGACCGGCACGAAGTAAAACCACCGCATCTTTTCGTCGAAAAG
CATCTAAAAGGAGTTTGACC

Downstream 100 bases:

>100_bases
TCCACGCACTGTTCTTATCACTGGTGCCGCTGGCGGTTTGGGTCGGGCATTCGCTGAAGGTTTCGCAGCCCAAGGAGACC
GTATCGCGGTGGCGGATATC

Product: hypothetical protein

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MANKSFPKPSDLPVPRGAEGWEDLYPYYLVFQDKLMDEENEKFWFCDSQHWPTVFKPFETIGGEFAVKCLGQYNARHLMI
PNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITHYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIYDI
VSGKAKDGTEVLMENFDRLIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDELKI
LAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHDKYWIEHLELPLGYIADYIRR
LDEGQTISRPKDELIAEKDRVVEEYRDLLDGEQLAQFDAKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQGY
GFWENEEDLLYLNRTEVRDVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTRML
WGITTEQVQSWLGNDEDTEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVTAPSWGPIFGKIKATVTDIGG
MMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKGKVVIVDPDAPRIEGPGAHSHAHEVAAHGVDTHA

Sequences:

>Translated_633_residues
MANKSFPKPSDLPVPRGAEGWEDLYPYYLVFQDKLMDEENEKFWFCDSQHWPTVFKPFETIGGEFAVKCLGQYNARHLMI
PNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITHYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIYDI
VSGKAKDGTEVLMENFDRLIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDELKI
LAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHDKYWIEHLELPLGYIADYIRR
LDEGQTISRPKDELIAEKDRVVEEYRDLLDGEQLAQFDAKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQGY
GFWENEEDLLYLNRTEVRDVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTRML
WGITTEQVQSWLGNDEDTEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVTAPSWGPIFGKIKATVTDIGG
MMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKGKVVIVDPDAPRIEGPGAHSHAHEVAAHGVDTHA
>Mature_632_residues
ANKSFPKPSDLPVPRGAEGWEDLYPYYLVFQDKLMDEENEKFWFCDSQHWPTVFKPFETIGGEFAVKCLGQYNARHLMIP
NANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITHYFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIYDIV
SGKAKDGTEVLMENFDRLIQLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDELKIL
AQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHDKYWIEHLELPLGYIADYIRRL
DEGQTISRPKDELIAEKDRVVEEYRDLLDGEQLAQFDAKCGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQGYG
FWENEEDLLYLNRTEVRDVLFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTRMLW
GITTEQVQSWLGNDEDTEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVTAPSWGPIFGKIKATVTDIGGM
MSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKGKVVIVDPDAPRIEGPGAHSHAHEVAAHGVDTHA

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17564524, Length=106, Percent_Identity=36.7924528301887, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 71608; Mature: 71476

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKSFPKPSDLPVPRGAEGWEDLYPYYLVFQDKLMDEENEKFWFCDSQHWPTVFKPFET
CCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEHHHCCCCCCEEEEECCCCCCHHHHHHHH
IGGEFAVKCLGQYNARHLMIPNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITH
CCHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEEEEECCCCCCHHHHHHHCHHHHHHHHH
YFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIYDIVSGKAKDGTEVLMENFDRLI
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
QLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDELKI
HHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCHHHHH
LAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCH
KYWIEHLELPLGYIADYIRRLDEGQTISRPKDELIAEKDRVVEEYRDLLDGEQLAQFDAK
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHC
CGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQGYGFWENEEDLLYLNRTEVRDV
CCCEEEECCEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHH
LFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTRML
HHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
WGITTEQVQSWLGNDEDTEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVT
HCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCEEEEEEC
APSWGPIFGKIKATVTDIGGMMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCEEEECCCCC
KVVIVDPDAPRIEGPGAHSHAHEVAAHGVDTHA
EEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANKSFPKPSDLPVPRGAEGWEDLYPYYLVFQDKLMDEENEKFWFCDSQHWPTVFKPFET
CCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEHHHCCCCCCEEEEECCCCCCHHHHHHHH
IGGEFAVKCLGQYNARHLMIPNANGIEFRVHLGYLYMSPIPVPEDQIAERVPMFQERITH
CCHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEEEEECCCCCCHHHHHHHCHHHHHHHHH
YFQNWEPMLANWKERVLGTINELESLEFKPLPDYVPIYDIVSGKAKDGTEVLMENFDRLI
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
QLAYQNWQYHFEFLNLGYIAYLDFFNFCKEVFPDIPDQSISMMVQGVDMELFRPDDELKI
HHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCHHHHH
LAQLAVDLGLQTHFANPDDPQATLAAIAKAEGGATWIARWEEAQDPWFNFTVGNGFYGHD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCH
KYWIEHLELPLGYIADYIRRLDEGQTISRPKDELIAEKDRVVEEYRDLLDGEQLAQFDAK
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHC
CGLAATAYPYVENHNFYIEHWTMSVFWRKVRELSRTLQGYGFWENEEDLLYLNRTEVRDV
CCCEEEECCEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHH
LFDLATAWGVGAPGGPIGTIIWPEEIERRKAIVTALKTARPAPALNTPPESITEPFTRML
HHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
WGITTEQVQSWLGNDEDTEEGTLKGMAASPGVVEGYARVILSADDLSEIQQDEILVAPVT
HCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCEEEEEEC
APSWGPIFGKIKATVTDIGGMMSHAAIVCREYGLPAVTGTGAASTTIKTGDYLKVDGTKG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCEEEECCCCC
KVVIVDPDAPRIEGPGAHSHAHEVAAHGVDTHA
EEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]