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The map label for this gene is pit [H]

Identifier: 145294582

GI number: 145294582

Start: 590564

End: 591829

Strand: Reverse

Name: pit [H]

Synonym: cgR_0533

Alternate gene names: 145294582

Gene position: 591829-590564 (Counterclockwise)

Preceding gene: 145294585

Following gene: 145294581

Centisome position: 17.86

GC content: 55.06

Gene sequence:

>1266_bases
GTGACCGAGCTGATTATTTTATTGATTGTTATCGTTACGGCGCTAGCCTTCGATTTCACAAACGGATTCCACGACACGGG
CAATGCGATGGCCACATCCATTGCCACAGGCGCTCTCAAACCTAAAGTCGCCGTGGCACTATCCGCCGCACTAAACCTTG
TTGGCGCATTCCTCTCTGTAGAAGTTGCGACAACCGTCGCAAAAGGCGTTGTTGACCTCGACCAATTCGATCTAAGCAAT
GCCTGGGATTCCCACCAGCTCCTGCTCGTCGTCTTTGCTGGCCTCATTGGCGCCATCGTCTGGAACCTTCTGACCTGGCT
GCTAGGCATTCCTTCCAGCTCCTCTCACGCACTTTTCGGTGGCCTCATTGGTGCCGCAATTGCTTCACTCGGTTTCGGCG
GAGTGGTGTGGGAAGGTGTCTTGTCCAAGATGATCATCCCCGCGTTGGCTGCACCAGTTGTTGCAGGTCTTGTCGCCGCA
ATCGGCACTTTCGCTGTGTACAGCATCACCAAAGCAGTTGGAGACAACGAGAAGAACCGTTACTTCCGCTGGGGTCAGAT
TGGCTCTGCTTCCCTCGTTTCCCTGGCCCACGGAACCAACGATGCCCAGAAAACCATGGGCGTTATCTTCCTTTCCCTTG
TTGCCACCGGCCACCTAGGAACTGACGCCGACATCCCATTCTGGGTGAAGGCTACATGTGCGTTGGCTATCGCAATCGGA
ACCTACCTAGGCGGTTGGCGCGTTATCCGCACCCTGGGCAAAGGCTTGGTTGAGATTGATTCTCCTCAGGGCATGGCAGC
AGAAACTTCTTCCGCAGCAATCATCTTGACTTCCTCCCACTTCGGTATGGCCCTGTCAACCACCCACGTTGCAACTGGCT
CCATCATGGGTACCGGTCTCGGACGAAAAGGCGCGAAGGTTCGCTGGTCTGTTGCAGGACGCATGGCGATGGCCTGGATC
ATCACTCTCCCAGCTTCCGCGATCGTTGGCGTTTTCTGCTGGTGGATAGCTCACGGAATCGGTCTTATCAGCTCAGATCT
TCTCGGTGTCCTCGTTGCATTCGCCATTCTGGTCTCCCTGTCCGGCTACATTTACGCCCGTTCCCGTCGCGTGCCGGTTG
ATCCAAGCAACGTCAATGCTGACTGGAATGAAGAATCAAATAGCGTGGAGCCTGCAGCCCCGTCTACCCCAGCTGCTCCT
AAGGCTACAGAAGCTGCCGAAGCACCTGCTGTTCAAGATCTCAACGAGAATGAGGTAACCAAGTAA

Upstream 100 bases:

>100_bases
CCTAATCATGGGGATTTCGAACTCTACCCTGGGGATTTGCCGTCAGAAAAAACAAAACCGACGTTTGGTTTTGGCACCTT
TTAGGGCATACTTCCCAATC

Downstream 100 bases:

>100_bases
TGAACTTCGCTACTATTTTCGGCAGCATCTTTGAGGTCACTCTCGTGGGCATTTTGCTCGGCGCAGGCCTTCCCGCTCTC
TTCGCATTGGGAATCCGCTT

Product: hypothetical protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSAALNLVGAFLSVEVATTVAKGVVDLDQFDLSN
AWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFGGLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAA
IGTFAVYSITKAVGDNEKNRYFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGLGRKGAKVRWSVAGRMAMAWI
ITLPASAIVGVFCWWIAHGIGLISSDLLGVLVAFAILVSLSGYIYARSRRVPVDPSNVNADWNEESNSVEPAAPSTPAAP
KATEAAEAPAVQDLNENEVTK

Sequences:

>Translated_421_residues
MTELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSAALNLVGAFLSVEVATTVAKGVVDLDQFDLSN
AWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFGGLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAA
IGTFAVYSITKAVGDNEKNRYFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGLGRKGAKVRWSVAGRMAMAWI
ITLPASAIVGVFCWWIAHGIGLISSDLLGVLVAFAILVSLSGYIYARSRRVPVDPSNVNADWNEESNSVEPAAPSTPAAP
KATEAAEAPAVQDLNENEVTK
>Mature_420_residues
TELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSAALNLVGAFLSVEVATTVAKGVVDLDQFDLSNA
WDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFGGLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAAI
GTFAVYSITKAVGDNEKNRYFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIGT
YLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGLGRKGAKVRWSVAGRMAMAWII
TLPASAIVGVFCWWIAHGIGLISSDLLGVLVAFAILVSLSGYIYARSRRVPVDPSNVNADWNEESNSVEPAAPSTPAAPK
ATEAAEAPAVQDLNENEVTK

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=150, Percent_Identity=34, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI5803173, Length=149, Percent_Identity=30.8724832214765, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789360, Length=223, Percent_Identity=33.6322869955157, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1789907, Length=224, Percent_Identity=34.375, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=30.8641975308642, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17539280, Length=157, Percent_Identity=29.2993630573248, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25146401, Length=146, Percent_Identity=32.1917808219178, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=159, Percent_Identity=28.9308176100629, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI32566716, Length=168, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=28.4810126582279, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356511, Length=180, Percent_Identity=28.8888888888889, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 43563; Mature: 43432

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSAALNLVGAFLSV
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EVATTVAKGVVDLDQFDLSNAWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAAIGTFAVYSITKAVGDNEKNR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
EEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGL
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCCEEEEHHHHCCCCCEECCC
GRKGAKVRWSVAGRMAMAWIITLPASAIVGVFCWWIAHGIGLISSDLLGVLVAFAILVSL
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGYIYARSRRVPVDPSNVNADWNEESNSVEPAAPSTPAAPKATEAAEAPAVQDLNENEVT
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCC
K
C
>Mature Secondary Structure 
TELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSAALNLVGAFLSV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EVATTVAKGVVDLDQFDLSNAWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAAIGTFAVYSITKAVGDNEKNR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
EEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGL
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCCEEEEHHHHCCCCCEECCC
GRKGAKVRWSVAGRMAMAWIITLPASAIVGVFCWWIAHGIGLISSDLLGVLVAFAILVSL
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGYIYARSRRVPVDPSNVNADWNEESNSVEPAAPSTPAAPKATEAAEAPAVQDLNENEVT
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]