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The map label for this gene is potD [C]

Identifier: 145294547

GI number: 145294547

Start: 556120

End: 557199

Strand: Reverse

Name: potD [C]

Synonym: cgR_0498

Alternate gene names: 145294547

Gene position: 557199-556120 (Counterclockwise)

Preceding gene: 145294567

Following gene: 145294546

Centisome position: 16.81

GC content: 54.54

Gene sequence:

>1080_bases
ATGTCTTCGAAGCACCCTTTGAAGCGCACCGCTACTACCGTTTTCGCACTCGGCGCGTCCGCTGCTCTCCTCGTGGCTTG
CTCTGAACCTTCTGAGGACGTTTCCACCGCAGAGACCACCACTGCAAGCTCTTCCGCTAACGCATCCGATGCAGCCGGTG
AAAAAGTAACCATCACCGTCTACACCTCTGAGCCTGAGGAAAAGGTCGATGAGATCAACAAGGCGTTCATGGAAGCCAAC
CCAGATATTGAGGTTGAGGTGTACCGCGCTGGTACTGGCGATCTGACTGCTCGCATTGAAGCTGAAAAGGCATCCGGTTC
TATCGAGGCTGATGTGTTGTGGGCTGCGGATGCTGCAACCTTTGAAACTTATGCAGCACAGGGCGACCTTGCAGAGCTGG
AAGATGTTGATACTTCCGACCTCATTGAAGAGGCTCTGGATGCTGAGAACTTTTATGTAGGCACCCGCATCATCCCAACC
GTGATTGCATACAACACTGAAGTTGTTGATCAGGCGGAGCTTCCTACGTCTTGGGCTGATCTGACTGATCCTAAGTATGC
AGGCCAACTAGTTATGCCGGATCCTGCTGTGTCTGGTGCTGCAGCGTTCAATGCTTCTGTGTGGAAGAACGACCCTGCGC
TTGGCGAAGTCTGGATCACCGCCTTGGGTGAAAACCAACCAATGATCGCTCAGTCCAACGGCCCAACCTCCCAGGAGATC
GCTGGCGGTGGCCACCCAGTGGGCATCGTGGTGGACTACTTGGTGCGCGACTTGGCTGCTGCTGGATCTCCAATCGACAC
CATCTACGCATCGGAGGGTTCCCCTTACATCACTGAGCCTGCAGGTGTGTTCGCTGATTCTGAAAAGAAGGAAGCAGCCG
AGCGCTACATCAACTTCCTGCTGTCTGTTGAAGGCCAGGAAATCGCAGTTGAGCAGGCATACCTGCCAGTGCGTGAAGAT
GTCGGAACTCCAGAGGGCACCCCCGAGTTGGCTGACATCGAGCTCATGACCCCTGACCTGGAGGTTGTAACCGCTGATAA
GGCGGCTGCTGTTGAGTTCTTCCAAAACGCAATGAACTAG

Upstream 100 bases:

>100_bases
CATTTCCATAAGTCCTGCGAAATGCACCCATTCATGTAAAGATGTTATTTGCCTCCCCCACAACCACTCCCTTAAAATTT
CAAGAAGGGACTGATTTTTC

Downstream 100 bases:

>100_bases
TTTTCCTATGCAGTTATCTCCTTCAAATACGTCGACGCAGCGCTTTCACATCTCTGCGTTGGCGTCTTTGTCTTTCATGC
GTCTGGGTATGTGGCTGATT

Product: hypothetical protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSSKHPLKRTATTVFALGASAALLVACSEPSEDVSTAETTTASSSANASDAAGEKVTITVYTSEPEEKVDEINKAFMEAN
PDIEVEVYRAGTGDLTARIEAEKASGSIEADVLWAADAATFETYAAQGDLAELEDVDTSDLIEEALDAENFYVGTRIIPT
VIAYNTEVVDQAELPTSWADLTDPKYAGQLVMPDPAVSGAAAFNASVWKNDPALGEVWITALGENQPMIAQSNGPTSQEI
AGGGHPVGIVVDYLVRDLAAAGSPIDTIYASEGSPYITEPAGVFADSEKKEAAERYINFLLSVEGQEIAVEQAYLPVRED
VGTPEGTPELADIELMTPDLEVVTADKAAAVEFFQNAMN

Sequences:

>Translated_359_residues
MSSKHPLKRTATTVFALGASAALLVACSEPSEDVSTAETTTASSSANASDAAGEKVTITVYTSEPEEKVDEINKAFMEAN
PDIEVEVYRAGTGDLTARIEAEKASGSIEADVLWAADAATFETYAAQGDLAELEDVDTSDLIEEALDAENFYVGTRIIPT
VIAYNTEVVDQAELPTSWADLTDPKYAGQLVMPDPAVSGAAAFNASVWKNDPALGEVWITALGENQPMIAQSNGPTSQEI
AGGGHPVGIVVDYLVRDLAAAGSPIDTIYASEGSPYITEPAGVFADSEKKEAAERYINFLLSVEGQEIAVEQAYLPVRED
VGTPEGTPELADIELMTPDLEVVTADKAAAVEFFQNAMN
>Mature_358_residues
SSKHPLKRTATTVFALGASAALLVACSEPSEDVSTAETTTASSSANASDAAGEKVTITVYTSEPEEKVDEINKAFMEANP
DIEVEVYRAGTGDLTARIEAEKASGSIEADVLWAADAATFETYAAQGDLAELEDVDTSDLIEEALDAENFYVGTRIIPTV
IAYNTEVVDQAELPTSWADLTDPKYAGQLVMPDPAVSGAAAFNASVWKNDPALGEVWITALGENQPMIAQSNGPTSQEIA
GGGHPVGIVVDYLVRDLAAAGSPIDTIYASEGSPYITEPAGVFADSEKKEAAERYINFLLSVEGQEIAVEQAYLPVREDV
GTPEGTPELADIELMTPDLEVVTADKAAAVEFFQNAMN

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37882; Mature: 37751

Theoretical pI: Translated: 3.69; Mature: 3.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKHPLKRTATTVFALGASAALLVACSEPSEDVSTAETTTASSSANASDAAGEKVTITV
CCCCCCCHHHHHHHHEECCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEE
YTSEPEEKVDEINKAFMEANPDIEVEVYRAGTGDLTARIEAEKASGSIEADVLWAADAAT
EECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEEEECCCHH
FETYAAQGDLAELEDVDTSDLIEEALDAENFYVGTRIIPTVIAYNTEVVDQAELPTSWAD
HHHHHCCCCHHHHHCCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCCC
LTDPKYAGQLVMPDPAVSGAAAFNASVWKNDPALGEVWITALGENQPMIAQSNGPTSQEI
CCCCHHCCEEECCCCCCCCCHHCCCHHCCCCCCCCEEEEEEECCCCCEEEECCCCCHHHH
AGGGHPVGIVVDYLVRDLAAAGSPIDTIYASEGSPYITEPAGVFADSEKKEAAERYINFL
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHH
LSVEGQEIAVEQAYLPVREDVGTPEGTPELADIELMTPDLEVVTADKAAAVEFFQNAMN
HCCCCCEEEEEHHCCCHHHHCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SSKHPLKRTATTVFALGASAALLVACSEPSEDVSTAETTTASSSANASDAAGEKVTITV
CCCCCCHHHHHHHHEECCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEE
YTSEPEEKVDEINKAFMEANPDIEVEVYRAGTGDLTARIEAEKASGSIEADVLWAADAAT
EECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEEEECCCHH
FETYAAQGDLAELEDVDTSDLIEEALDAENFYVGTRIIPTVIAYNTEVVDQAELPTSWAD
HHHHHCCCCHHHHHCCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCCC
LTDPKYAGQLVMPDPAVSGAAAFNASVWKNDPALGEVWITALGENQPMIAQSNGPTSQEI
CCCCHHCCEEECCCCCCCCCHHCCCHHCCCCCCCCEEEEEEECCCCCEEEECCCCCHHHH
AGGGHPVGIVVDYLVRDLAAAGSPIDTIYASEGSPYITEPAGVFADSEKKEAAERYINFL
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHH
LSVEGQEIAVEQAYLPVREDVGTPEGTPELADIELMTPDLEVVTADKAAAVEFFQNAMN
HCCCCCEEEEEHHCCCHHHHCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]