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The map label for this gene is alsT [H]

Identifier: 145294330

GI number: 145294330

Start: 311718

End: 313205

Strand: Reverse

Name: alsT [H]

Synonym: cgR_0285

Alternate gene names: 145294330

Gene position: 313205-311718 (Counterclockwise)

Preceding gene: 145294332

Following gene: 145294328

Centisome position: 9.45

GC content: 52.82

Gene sequence:

>1488_bases
ATGGAATTATTGGAGACCTTCATCACTGATGTCATAAATGACAATTTGTGGATGATCTTGCCCTTCTTGCTCGTTGCTGC
TGGCCTCTATTTCGGTGGGCGTACGTTGCTGGTTCAGATTCGGATGATTCCGGAGATGTTCAAAGCGGTCGTCGAGAAGC
CTGCGAAGGATGGGGAGTTCGCGGACAAGCAGGACATTTCGGCTTTTAAGGCGTTCACGATTTCTGCGGCGTCGCGAGTT
GGTACGGCGAATGTTGCGGGTGTTGCGCTGGCGATCACTCTGGGTGGACCGGGTGCAGTGTTCTGGATGTGGATCATTGC
GCTGGTTGGCGGTGCGACATCGTTCATTGAGTCGACTCTTGGACAGTTGTGGAAGGTGAAGGACGGCGACAGCTATCGCG
GTGGCCCTGCGTACTACATGACGCTTGGTTTGAATGCTCGGTGGCTTGCGGTTGTTTTCGGTGTCGCCATCACGTTGACC
TTTGGTTTTGTGTACAACGCTTTGCAGTCCAACGCGGTTGTTGAGGCGATTACTGTATCACTCGGCACCCCTTCTACCAC
TGCAAAGGCTTTTGTTGGCTTGGGCATGGCTGGATTGTCTGCGCTGGTTATTTTTGGCGGCGTGCAGCGTATCGCAAACG
TGACGCAGTGGATGGTTCCGTTCATGGCGGGTGCGTACATCATTGTTGGTGTGGTGGTGATTGTGATTAACATTCAGCAG
GTTCCGACCATGATCAACGACATCATTGCTGGTGCTTTTGGTTTCCGTCCGGTTGCTACTGCGTCGGTGTGGGGTGCGTT
CTGGTTGGCGTTTATGAACGGTATGCGCCGTGGACTGTTCTCCAATGAGGCTGGTGAGGGTTCTGTCCCGAACGCTGCTG
CTACCGCGACTGTGTCTCACCCTGTGAAGCAGGGTTTGGTTCAGACTCTGGGCGTATATTTCGATACCCTGCTCGTTTGT
AGCATTACTGCTTTTGTCATCCTGCTGTCTGGAGTGGAGTACGCGACCGGCGATATTCAGTCTTCTTCTTTGACTCAGTC
CGCGCTGGCTAGCGTTGTTGGTGGTTGGGGAACCCACTTCATTACCGCAGTGATGTTCTTCCTGGCGTTTTCTTCCGTGC
TGGGTAACTACTACTTGGCGCAGGCAAATATTCAGTACTTCACCGATTCGAAGACTGTCATGACTGTTTTCCGACTCTTG
GTGCTGCTCAGTGTGTTCTCTGGCGCGGTTGCTTCGGTGCCGTTGATCTGGGCTTTGGGAGATACTTTCGCGGGCATCAT
GGTGCTCATTAACCTGGCGGCGATCATTCCGCTGAGTGGCGTTGCCGTGAAATTGCTCAAGAATTACACCACTCAGAAGA
AGGCTGGCTTGGATCCTGTGTTCCACCGCGACATGATGCCAGAGGTCCGCAATATTGCGTGCTGGAATGGCAAAGATGCA
GCTACGTCCAACTATCACGAAGCAATGGAAGTGATCAAGAAGAATTAG

Upstream 100 bases:

>100_bases
AGCTAATCTGCCTTGCTCTACACCTCCGGGCTATAACAATCCAGTGTCAGTGGCTAGGGATTTACTAAAAATCGGGTAAC
ACGCGCGTAGTATTTTTCGC

Downstream 100 bases:

>100_bases
TCATCGAAGGAACAATGGTATGGCCCGTCAGCGTCTCGGATTGTCTGGCAGGCCAACACTTGGTTTTCGGAGACGATCTC
GTAGTTGCTCAGTACTTCAT

Product: hypothetical protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MELLETFITDVINDNLWMILPFLLVAAGLYFGGRTLLVQIRMIPEMFKAVVEKPAKDGEFADKQDISAFKAFTISAASRV
GTANVAGVALAITLGGPGAVFWMWIIALVGGATSFIESTLGQLWKVKDGDSYRGGPAYYMTLGLNARWLAVVFGVAITLT
FGFVYNALQSNAVVEAITVSLGTPSTTAKAFVGLGMAGLSALVIFGGVQRIANVTQWMVPFMAGAYIIVGVVVIVINIQQ
VPTMINDIIAGAFGFRPVATASVWGAFWLAFMNGMRRGLFSNEAGEGSVPNAAATATVSHPVKQGLVQTLGVYFDTLLVC
SITAFVILLSGVEYATGDIQSSSLTQSALASVVGGWGTHFITAVMFFLAFSSVLGNYYLAQANIQYFTDSKTVMTVFRLL
VLLSVFSGAVASVPLIWALGDTFAGIMVLINLAAIIPLSGVAVKLLKNYTTQKKAGLDPVFHRDMMPEVRNIACWNGKDA
ATSNYHEAMEVIKKN

Sequences:

>Translated_495_residues
MELLETFITDVINDNLWMILPFLLVAAGLYFGGRTLLVQIRMIPEMFKAVVEKPAKDGEFADKQDISAFKAFTISAASRV
GTANVAGVALAITLGGPGAVFWMWIIALVGGATSFIESTLGQLWKVKDGDSYRGGPAYYMTLGLNARWLAVVFGVAITLT
FGFVYNALQSNAVVEAITVSLGTPSTTAKAFVGLGMAGLSALVIFGGVQRIANVTQWMVPFMAGAYIIVGVVVIVINIQQ
VPTMINDIIAGAFGFRPVATASVWGAFWLAFMNGMRRGLFSNEAGEGSVPNAAATATVSHPVKQGLVQTLGVYFDTLLVC
SITAFVILLSGVEYATGDIQSSSLTQSALASVVGGWGTHFITAVMFFLAFSSVLGNYYLAQANIQYFTDSKTVMTVFRLL
VLLSVFSGAVASVPLIWALGDTFAGIMVLINLAAIIPLSGVAVKLLKNYTTQKKAGLDPVFHRDMMPEVRNIACWNGKDA
ATSNYHEAMEVIKKN
>Mature_495_residues
MELLETFITDVINDNLWMILPFLLVAAGLYFGGRTLLVQIRMIPEMFKAVVEKPAKDGEFADKQDISAFKAFTISAASRV
GTANVAGVALAITLGGPGAVFWMWIIALVGGATSFIESTLGQLWKVKDGDSYRGGPAYYMTLGLNARWLAVVFGVAITLT
FGFVYNALQSNAVVEAITVSLGTPSTTAKAFVGLGMAGLSALVIFGGVQRIANVTQWMVPFMAGAYIIVGVVVIVINIQQ
VPTMINDIIAGAFGFRPVATASVWGAFWLAFMNGMRRGLFSNEAGEGSVPNAAATATVSHPVKQGLVQTLGVYFDTLLVC
SITAFVILLSGVEYATGDIQSSSLTQSALASVVGGWGTHFITAVMFFLAFSSVLGNYYLAQANIQYFTDSKTVMTVFRLL
VLLSVFSGAVASVPLIWALGDTFAGIMVLINLAAIIPLSGVAVKLLKNYTTQKKAGLDPVFHRDMMPEVRNIACWNGKDA
ATSNYHEAMEVIKKN

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=463, Percent_Identity=37.1490280777538, Blast_Score=287, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52810; Mature: 52810

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLETFITDVINDNLWMILPFLLVAAGLYFGGRTLLVQIRMIPEMFKAVVEKPAKDGEF
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADKQDISAFKAFTISAASRVGTANVAGVALAITLGGPGAVFWMWIIALVGGATSFIESTL
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHH
GQLWKVKDGDSYRGGPAYYMTLGLNARWLAVVFGVAITLTFGFVYNALQSNAVVEAITVS
HHHEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEE
LGTPSTTAKAFVGLGMAGLSALVIFGGVQRIANVTQWMVPFMAGAYIIVGVVVIVINIQQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPTMINDIIAGAFGFRPVATASVWGAFWLAFMNGMRRGLFSNEAGEGSVPNAAATATVSH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
PVKQGLVQTLGVYFDTLLVCSITAFVILLSGVEYATGDIQSSSLTQSALASVVGGWGTHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCHHHHH
ITAVMFFLAFSSVLGNYYLAQANIQYFTDSKTVMTVFRLLVLLSVFSGAVASVPLIWALG
HHHHHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTFAGIMVLINLAAIIPLSGVAVKLLKNYTTQKKAGLDPVFHRDMMPEVRNIACWNGKDA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHCEECCCCCCC
ATSNYHEAMEVIKKN
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MELLETFITDVINDNLWMILPFLLVAAGLYFGGRTLLVQIRMIPEMFKAVVEKPAKDGEF
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADKQDISAFKAFTISAASRVGTANVAGVALAITLGGPGAVFWMWIIALVGGATSFIESTL
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHH
GQLWKVKDGDSYRGGPAYYMTLGLNARWLAVVFGVAITLTFGFVYNALQSNAVVEAITVS
HHHEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEE
LGTPSTTAKAFVGLGMAGLSALVIFGGVQRIANVTQWMVPFMAGAYIIVGVVVIVINIQQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPTMINDIIAGAFGFRPVATASVWGAFWLAFMNGMRRGLFSNEAGEGSVPNAAATATVSH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
PVKQGLVQTLGVYFDTLLVCSITAFVILLSGVEYATGDIQSSSLTQSALASVVGGWGTHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCHHHHH
ITAVMFFLAFSSVLGNYYLAQANIQYFTDSKTVMTVFRLLVLLSVFSGAVASVPLIWALG
HHHHHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTFAGIMVLINLAAIIPLSGVAVKLLKNYTTQKKAGLDPVFHRDMMPEVRNIACWNGKDA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHCEECCCCCCC
ATSNYHEAMEVIKKN
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]