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The map label for this gene is tnpR [H]

Identifier: 145294203

GI number: 145294203

Start: 176226

End: 176858

Strand: Reverse

Name: tnpR [H]

Synonym: cgR_0160

Alternate gene names: 145294203

Gene position: 176858-176226 (Counterclockwise)

Preceding gene: 145294206

Following gene: 145294202

Centisome position: 5.34

GC content: 61.61

Gene sequence:

>633_bases
GTGGTCATGGATATTGGTTACGCCCGGGTTTCCACCGCGAAACAAGACCTCGACCGCCAGATCGATGCGCTCCGTAAAGA
AGGTGTTGCGGATAAATACATCTACGTCGACAAGAAATCCGGATCCACCACTAATCGGCCCGGACTTCAGCAAGCCCTTG
AGCAAGCCCGCGCGGGTGATGTCATCGTCGTTCACACCCTCGACCGGCTGGGCCGCACCGTCCGGGACACTCTCAACTTG
ATCCATGATCTTCAGGAGCGTGAGGTCGGGGTGCGCACCCTGGCCGATCCCATCAAGGTCGATTCCTCGAATCCGAATGA
TCCGATGGCGCAGTTGGCGGTGGTGATCCTCTCGCTGTTCGGGCAGATGGAACGCACCTACGCCGTCGAGCGCGCAGCCC
ATGCCCGGGCAGTGGCCGAAGCTAAGGGAAAACGGGTGGGCCGGCCCAGTGTCGTGGACCCGGACAAACTCCGATACGCC
GAACACTTGCGCAACGAGGGTTACTCGATGAGCAAGATCGTGGAGAAGACCGGCATCACCCGCTCCAGCCTCTACCGGCA
CCTGCCACCCCGGCCGGTGGAAACCCACACCGCCGCAGACATCCCTGACGCCTCACACCCCACCTTGGGGTGA

Upstream 100 bases:

>100_bases
AACCAATTGAAAATGCATAGTCCCAAGAGAAAACTGTTCCACAAGCTATTCCAACGAACTGTCTCACGAACCCTGCTACA
GAATAGACTTCCGGTACGCT

Downstream 100 bases:

>100_bases
CGGTTGGTGAACAGGACTTCTGACACTTGTCGTCTTGTGCACGCGGGTAGTGACCAGATAGTGGTCAGAAGGCGCGTGCA
CAGCCCTGCTCACCACATCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MVMDIGYARVSTAKQDLDRQIDALRKEGVADKYIYVDKKSGSTTNRPGLQQALEQARAGDVIVVHTLDRLGRTVRDTLNL
IHDLQEREVGVRTLADPIKVDSSNPNDPMAQLAVVILSLFGQMERTYAVERAAHARAVAEAKGKRVGRPSVVDPDKLRYA
EHLRNEGYSMSKIVEKTGITRSSLYRHLPPRPVETHTAADIPDASHPTLG

Sequences:

>Translated_210_residues
MVMDIGYARVSTAKQDLDRQIDALRKEGVADKYIYVDKKSGSTTNRPGLQQALEQARAGDVIVVHTLDRLGRTVRDTLNL
IHDLQEREVGVRTLADPIKVDSSNPNDPMAQLAVVILSLFGQMERTYAVERAAHARAVAEAKGKRVGRPSVVDPDKLRYA
EHLRNEGYSMSKIVEKTGITRSSLYRHLPPRPVETHTAADIPDASHPTLG
>Mature_210_residues
MVMDIGYARVSTAKQDLDRQIDALRKEGVADKYIYVDKKSGSTTNRPGLQQALEQARAGDVIVVHTLDRLGRTVRDTLNL
IHDLQEREVGVRTLADPIKVDSSNPNDPMAQLAVVILSLFGQMERTYAVERAAHARAVAEAKGKRVGRPSVVDPDKLRYA
EHLRNEGYSMSKIVEKTGITRSSLYRHLPPRPVETHTAADIPDASHPTLG

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=187, Percent_Identity=36.3636363636364, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787638, Length=186, Percent_Identity=27.4193548387097, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787827, Length=190, Percent_Identity=27.3684210526316, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23243; Mature: 23243

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMDIGYARVSTAKQDLDRQIDALRKEGVADKYIYVDKKSGSTTNRPGLQQALEQARAGD
CEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCC
VIVVHTLDRLGRTVRDTLNLIHDLQEREVGVRTLADPIKVDSSNPNDPMAQLAVVILSLF
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
GQMERTYAVERAAHARAVAEAKGKRVGRPSVVDPDKLRYAEHLRNEGYSMSKIVEKTGIT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
RSSLYRHLPPRPVETHTAADIPDASHPTLG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVMDIGYARVSTAKQDLDRQIDALRKEGVADKYIYVDKKSGSTTNRPGLQQALEQARAGD
CEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCC
VIVVHTLDRLGRTVRDTLNLIHDLQEREVGVRTLADPIKVDSSNPNDPMAQLAVVILSLF
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
GQMERTYAVERAAHARAVAEAKGKRVGRPSVVDPDKLRYAEHLRNEGYSMSKIVEKTGIT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
RSSLYRHLPPRPVETHTAADIPDASHPTLG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]