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The map label for this gene is bioB

Identifier: 145294134

GI number: 145294134

Start: 103145

End: 104149

Strand: Direct

Name: bioB

Synonym: cgR_0092

Alternate gene names: 145294134

Gene position: 103145-104149 (Clockwise)

Preceding gene: 145294133

Following gene: 145294135

Centisome position: 3.11

GC content: 54.63

Gene sequence:

>1005_bases
ATGACAATCCCCGCCACCATCCTTGACACCGCCCGCACCCAAGTTCTGGAACAGGGAATTGGCCTTAATCAGCAGCAGTT
GATGGAGGTTCTCACCTTGCCTGAAGAGCAAATCCCAGACTTGATGGAATTAGCCCACCAGGTTCGGTTGAAGTGGTGTG
GAGAGGAAATCGAGGTAGAGGGCATTATTTCCCTCAAAACTGGCGGTTGCCCTGAAGATTGCCATTTCTGCTCACAGTCT
GGGTTGTTTGAATCGCCGGTGCGTTCGGTGTGGCTGGATATTCCGAATCTGGTTGAAGCCGCTAAACAGACCGCAAAAAC
TGGCGCTACCGAATTCTGTATCGTCGCCGCAGTCAAGGGGCCTGATGAGAGGCTCATGACCCAGCTGGAGGAAGCAGTCC
TCGCGATTCACTCTGAAGTTGAAATTGAAGTCGCAGCATCGATCGGAACGTTAAATAAGGAACAGGTGGATCGCCTCGCT
GCTGCCGGCGTGCACCGCTACAACCATAATTTGGAAACTGCGCGTTCCTATTTCCCTGAAGTTGTCACCACTCATACATG
GGAAGAGCGCCGCGAAACTTTGCGCCTGGTGGCAGAAGCTGGAATGGAAGTCTGTTCCGGCGGAATCTTAGGAATGGGCG
AAACTTTAGAGCAGCGCGCCGAGTTTGCCGTGCAGCTGGCGGAGCTTGATCCGCACGAAGTCCCCATGAACTTCCTTGAT
CCTCGCCCGGGCACCCCATTTGCCGATAGGGAATTGATGGACAGCCGTGACGCTCTGCGCTCTATTGGTGCGTTCCGCCT
TGCGATGCCTCACACCATGCTTCGTTTTGCTGGCGGTCGCGAGCTGACTTTGGGCGACAAGGGTTCCGAGCAAGCCCTCC
TGGGAGGCATCAATGCGATGATCGTCGGAAACTACCTGACCACGCTCGGCCGCCCAATGGAAGATGACCTCGACATGATG
GATCGTCTCCAGCTGCCCATCAAAGTCCTTAATAAGGTCATCTAA

Upstream 100 bases:

>100_bases
GTTTAAGGCACGTGTCATTTTGCATGCGCGTGCCCAATTCTTCTTAAGTGTGCTCAATTTTTAAGCGCGCAGTTATTGAC
AACCAGCCTCTAGGAGACCC

Downstream 100 bases:

>100_bases
GAAGCACGCGCATGAACGACGATCTCCAAATGGCCATGATTCTGGCAGACACCCCGATCTTTCATCCAAACACCGGCAAG
CCCTTCGATGGTGAACATAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTIPATILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVEGIISLKTGGCPEDCHFCSQS
GLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKGPDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLA
AAGVHRYNHNLETARSYFPEVVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAMIVGNYLTTLGRPMEDDLDMM
DRLQLPIKVLNKVI

Sequences:

>Translated_334_residues
MTIPATILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVEGIISLKTGGCPEDCHFCSQS
GLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKGPDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLA
AAGVHRYNHNLETARSYFPEVVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAMIVGNYLTTLGRPMEDDLDMM
DRLQLPIKVLNKVI
>Mature_333_residues
TIPATILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVEGIISLKTGGCPEDCHFCSQSG
LFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKGPDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLAA
AGVHRYNHNLETARSYFPEVVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLDP
RPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAMIVGNYLTTLGRPMEDDLDMMD
RLQLPIKVLNKVI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=304, Percent_Identity=37.828947368421, Blast_Score=208, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=29.7805642633229, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CORGB (A4QA10)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001136955.1
- ProteinModelPortal:   A4QA10
- SMR:   A4QA10
- STRING:   A4QA10
- GeneID:   4993228
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0092
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   ATGATEF
- ProtClustDB:   PRK06256
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36871; Mature: 36740

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPATILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVE
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCEEEEE
GIISLKTGGCPEDCHFCSQSGLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKG
EEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECC
PDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLAAAGVHRYNHNLETARSYFPE
CHHHHHHHHHHHHHHHHHHCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCH
VVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCHHHH
IVGNYLTTLGRPMEDDLDMMDRLQLPIKVLNKVI
HHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TIPATILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCEEEEE
GIISLKTGGCPEDCHFCSQSGLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKG
EEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECC
PDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLAAAGVHRYNHNLETARSYFPE
CHHHHHHHHHHHHHHHHHHCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCH
VVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCHHHH
IVGNYLTTLGRPMEDDLDMMDRLQLPIKVLNKVI
HHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA