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The map label for this gene is yiaE [C]

Identifier: 145294132

GI number: 145294132

Start: 101469

End: 102383

Strand: Reverse

Name: yiaE [C]

Synonym: cgR_0090

Alternate gene names: 145294132

Gene position: 102383-101469 (Counterclockwise)

Preceding gene: 145294137

Following gene: 145294129

Centisome position: 3.09

GC content: 51.91

Gene sequence:

>915_bases
ATGAAAATCTTTGTTGGTTTTGGCGATTATCCACTCACCACCAAGGCCCTTAAGGAGGCAGGCGCAGAAATAGTCGACTC
CCTCGAAAAAGCCGAGGGGTTTGTCTTCACTCAAACACCAGGCACAGAATTTCCCCTACTTCCCGACGGAGTGAGGTGGG
TGCAATTTCCCAATGCGGGGCTCAACGCATATTTCACAGCTGGGCAGATTGATGACAAACGCCGGTGGTCAAATGCATCA
GGGGTGTATGGCCAACAGGTAGCCGAAGCAGCAATGGGTCTTTTGCTGGGACTGATTCATATGCACCCCACCATGGTGCG
CGCCGATAGTTGGGCACCAAGAACTCACGTAGATCAGCAGACCAGATGGCTTGATGGCGCAATAGTTGCCATTGTGGGAG
CTGGTGGAATCGGTAAACATCTAGCAGCCATGTTGAAACCTTTTGGCGCAAAGTCTTTATCAGTAAGCAGGACCGGTACA
CCCACCCAAGATTTTGATGCAACGGAACCTATATCCAACCTGCACCAAGTACTTGCCGACGCCGACCATGTGGTGTTGTG
TGTACCGCTTACCGCAGACACCTATCATCTGATCGGAAAAGCAGAGCTTAAAGCAATGCAGTCTACTGCAATTTTGATCA
ACGTGGCTCGCGGAGAAGTAGTAGATACCGAAGCATTAGTTGACGCCTTAGATGCCCAAGAAATATCCGGGGCCGGCTTG
GATGTCACCGATCCTGAACCATTGCCGGACGATCATCCACTGTGGGGGCGCAGCAATGTGATCATTACCCCGCACGTAGC
CAACACCTTGACCTCGATGGATCGCATGCTTGCCCCAGTGGTGGCAGAAAACTACCGACGGTTGCTCGCTGGAGAAAAGA
TGCTGACCGAGGTGGACATCCACAAAGGTTACTAG

Upstream 100 bases:

>100_bases
AGAGATTGTTGATTCGGCACCAACTTTACCCACGAGGGTAGAAATGAAGCATTCAGGATGACAAAACCCAACCTCACACC
AACAACCTATCCTGGAGCCC

Downstream 100 bases:

>100_bases
AGCAGTGGCTTTGAATATAGATGTTCTGGTCGCCCGGACTGTCCGTACCGAAGACGAACCTGGATCACACCTTGGCTAGC
CATCGCTGCCAGCCTGCGCT

Product: hypothetical protein

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAGLNAYFTAGQIDDKRRWSNAS
GVYGQQVAEAAMGLLLGLIHMHPTMVRADSWAPRTHVDQQTRWLDGAIVAIVGAGGIGKHLAAMLKPFGAKSLSVSRTGT
PTQDFDATEPISNLHQVLADADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRLLAGEKMLTEVDIHKGY

Sequences:

>Translated_304_residues
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAGLNAYFTAGQIDDKRRWSNAS
GVYGQQVAEAAMGLLLGLIHMHPTMVRADSWAPRTHVDQQTRWLDGAIVAIVGAGGIGKHLAAMLKPFGAKSLSVSRTGT
PTQDFDATEPISNLHQVLADADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRLLAGEKMLTEVDIHKGY
>Mature_304_residues
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAGLNAYFTAGQIDDKRRWSNAS
GVYGQQVAEAAMGLLLGLIHMHPTMVRADSWAPRTHVDQQTRWLDGAIVAIVGAGGIGKHLAAMLKPFGAKSLSVSRTGT
PTQDFDATEPISNLHQVLADADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRLLAGEKMLTEVDIHKGY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=231, Percent_Identity=32.4675324675325, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI61743967, Length=171, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4557497, Length=171, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI145580578, Length=230, Percent_Identity=27.3913043478261, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4557499, Length=230, Percent_Identity=27.3913043478261, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI23308577, Length=212, Percent_Identity=24.5283018867925, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI145580575, Length=184, Percent_Identity=29.8913043478261, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI87082289, Length=197, Percent_Identity=32.994923857868, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081824, Length=179, Percent_Identity=32.4022346368715, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787645, Length=283, Percent_Identity=25.7950530035336, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789279, Length=154, Percent_Identity=33.1168831168831, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=215, Percent_Identity=30.2325581395349, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324055, Length=173, Percent_Identity=31.7919075144509, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322116, Length=233, Percent_Identity=28.3261802575107, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324980, Length=163, Percent_Identity=29.4478527607362, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=163, Percent_Identity=29.4478527607362, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=158, Percent_Identity=31.6455696202532, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=173, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571528, Length=192, Percent_Identity=35.9375, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI28574284, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585514, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574282, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45552429, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45551003, Length=227, Percent_Identity=33.4801762114537, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574286, Length=222, Percent_Identity=32.4324324324324, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585516, Length=225, Percent_Identity=31.1111111111111, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921140, Length=164, Percent_Identity=27.4390243902439, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646446, Length=237, Percent_Identity=28.2700421940928, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24646448, Length=237, Percent_Identity=28.2700421940928, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24646452, Length=237, Percent_Identity=28.2700421940928, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24646450, Length=237, Percent_Identity=28.2700421940928, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 32786; Mature: 32786

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCCC
LNAYFTAGQIDDKRRWSNASGVYGQQVAEAAMGLLLGLIHMHPTMVRADSWAPRTHVDQQ
CCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHH
TRWLDGAIVAIVGAGGIGKHLAAMLKPFGAKSLSVSRTGTPTQDFDATEPISNLHQVLAD
HHHHCCEEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHC
ADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
CCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCCEECHHHHHHHHHHHHHCCCCC
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRLLAGEKMLTEVDI
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEC
HKGY
CCCC
>Mature Secondary Structure
MKIFVGFGDYPLTTKALKEAGAEIVDSLEKAEGFVFTQTPGTEFPLLPDGVRWVQFPNAG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCCC
LNAYFTAGQIDDKRRWSNASGVYGQQVAEAAMGLLLGLIHMHPTMVRADSWAPRTHVDQQ
CCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHH
TRWLDGAIVAIVGAGGIGKHLAAMLKPFGAKSLSVSRTGTPTQDFDATEPISNLHQVLAD
HHHHCCEEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHC
ADHVVLCVPLTADTYHLIGKAELKAMQSTAILINVARGEVVDTEALVDALDAQEISGAGL
CCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCCEECHHHHHHHHHHHHHCCCCC
DVTDPEPLPDDHPLWGRSNVIITPHVANTLTSMDRMLAPVVAENYRRLLAGEKMLTEVDI
CCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEC
HKGY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]