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The map label for this gene is citS [H]

Identifier: 145294130

GI number: 145294130

Start: 99122

End: 100816

Strand: Direct

Name: citS [H]

Synonym: cgR_0088

Alternate gene names: 145294130

Gene position: 99122-100816 (Clockwise)

Preceding gene: 145294127

Following gene: 145294131

Centisome position: 2.99

GC content: 53.75

Gene sequence:

>1695_bases
TTGCTTCACGCTCTGCTGATAATCACCCCCGGGGGTAGGTATGTCTGTTGGTCGATCCGAACGAAAAACTTCAAGGAGGT
GGACATCATTCGCTTTGCTACCCGAATACTGGTGATTCAAGTGGCTACCGTCGCGTTGGTGGTAGCTATTTGCACCGGAA
TTTTCGCAGTTTTGATGATGGATCAGATGAAAACTGAGGCCGAGCACACAGCGCTGTCCATCGGACGTTCGGTGGCATCC
AACCCGCAGATACGCGAGGAAGTAGCGCTTGATACTCAAACAGGAGCAAACCCATCGGCCGAAGAATTAGCCGATGGAGA
TATCCAAGCGGTTGCACAGGCGGCCAATGAACGCACTGGAGCTTTGTTTGTCGTTATCACTGACGGTTTAGGTATCCGCC
TGTCCCACCCAGATGAGGAACGTCTGGGGGAGCAGGTGAGCACTAGCTTTGAGGCTGCCATGCGGGGTGAAGAAACCATG
GCGTGGGAGACTGGGACCCTCGGTGCGTCCGCGCGAGCAAAAGTGCCTATCTTTGCGCCGGATTCTAATGTTCCAGTCGG
TGAGGTCAGTGTTGGGTTTGAGCGAGACAGTGTGTATTCCCGCCTGCCCATGTTCCTCGCCGCCCTTGCTCTTATTTCTG
TGTTGGGAATTCTTATCGGCGTGGGTGTAGCCATGGGCATGCGACGCCGTTGGGAACGCGTGACCTTGGGTTTGCAGCCG
GAGGAGCTAGTGACCCTTGTGCAAAATCAGACTGCAGTCATCGATGGCATTGATGAGGGCGTGCTGGCGCTGAGCCCAAA
CGGAACAATTGGGGTGCATAATGAGCAGGCACAATCCATGATTGGTGCAGGTCCTATGAGTGGCAGGACGTTGAAAGAAC
TAGGGCTTGACCTGGGTCTTGATGGCGTTGTATTGCATGGTCAGCATCCGGAAACCGTTGCCCATAACGGCAGGATCCTC
TATCTGGATTTCCACCCCGTGCGCCGTGGGGATCAAGATTTAGGCTACGTGGTAACCATCCGCGATCGCACCGACATCAT
TGAACTCAGTGAACGCCTCGACTCTGTGCGCACCATGACCCACGCACTCCGCGCCCAGCGCCACGAGTTTGCCAACCGCA
TCCACACCGCAACAGGGCTTATCGACGCCGGCCGCGTCCACGACGCGGCAGAGTTTCTAGGCGATATATCCCGCAACGGG
GGACAGTCACATCCATTAATCGGATCAGCGCACCTCAATGAAGCATTTTTGAGCTCATTTTTAAGTACTGCTTCTATTTC
GGCATCTGAAAAGGGCGTTAGTCTGCGCATCAACTCTGACACGCTCATCCTTGGCACTGTTAAAGATCCAGAAGATGTAG
CAACCATTTTGGGTAATTTAATCAACAATGCCATCGACGCCGCGGTGTCAGGTGAAGCCCCACGGTGGATTGAGCTTACG
TTGATGGATGATGCCGATACGCTGGTCATTTCTGTTGCAGATTCTGGTCCTGGAATCCCAGAGGGCGTGGATGTATTTGC
CACAGCCACCCAGATAGGAGACTCTGAAGATAATGAACGCACCCACGGGCATGGCATTGGTCTAAAACTGTGCCGGGCTT
TGGCTAGATCACATGGTGGCGATGTCTGGGTCATTGATAGAGGAACCGAAGATGGCGCTGTATTTGGAGTGAAACTACCG
GGAGTAATGGAGTAA

Upstream 100 bases:

>100_bases
GGTGGCGTGTGTCACGCACTGAAGTGTTTGGTGCATTGCCTGGAGTAGTGCGCAAAAGAAGACTTTTGTGCATTATGATC
AGAATTGTTGGCCTGGGACT

Downstream 100 bases:

>100_bases
TGGATCAAACACTTAAAGTTTTAGTAATTGATGATGATTTCCGCGTCGCTGGCATTCACGCCTCCATCGTTGATGCGTCC
CCTGGATTTTCGGTGGTCGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MLHALLIITPGGRYVCWSIRTKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALSIGRSVAS
NPQIREEVALDTQTGANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETM
AWETGTLGASARAKVPIFAPDSNVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQP
EELVTLVQNQTAVIDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETVAHNGRIL
YLDFHPVRRGDQDLGYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNG
GQSHPLIGSAHLNEAFLSSFLSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVSGEAPRWIELT
LMDDADTLVISVADSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGAVFGVKLP
GVME

Sequences:

>Translated_564_residues
MLHALLIITPGGRYVCWSIRTKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALSIGRSVAS
NPQIREEVALDTQTGANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETM
AWETGTLGASARAKVPIFAPDSNVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQP
EELVTLVQNQTAVIDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETVAHNGRIL
YLDFHPVRRGDQDLGYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNG
GQSHPLIGSAHLNEAFLSSFLSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVSGEAPRWIELT
LMDDADTLVISVADSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGAVFGVKLP
GVME
>Mature_564_residues
MLHALLIITPGGRYVCWSIRTKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALSIGRSVAS
NPQIREEVALDTQTGANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETM
AWETGTLGASARAKVPIFAPDSNVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQP
EELVTLVQNQTAVIDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETVAHNGRIL
YLDFHPVRRGDQDLGYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNG
GQSHPLIGSAHLNEAFLSSFLSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVSGEAPRWIELT
LMDDADTLVISVADSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGAVFGVKLP
GVME

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=559, Percent_Identity=27.1914132379249, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1790567, Length=547, Percent_Identity=25.9597806215722, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI48994928, Length=156, Percent_Identity=32.6923076923077, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60215; Mature: 60215

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHALLIITPGGRYVCWSIRTKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMM
CCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQMKTEAEHTALSIGRSVASNPQIREEVALDTQTGANPSAEELADGDIQAVAQAANERTG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCC
ALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETMAWETGTLGASARAKVPIFAP
EEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECC
DSNVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQP
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
EELVTLVQNQTAVIDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGL
HHHHHHHCCCEEEEECCCCCEEEECCCCCEECCCHHHHHHHCCCCCCCCHHHHHCCCCCC
DGVVLHGQHPETVAHNGRILYLDFHPVRRGDQDLGYVVTIRDRTDIIELSERLDSVRTMT
CCEEEECCCCCHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
HALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNGGQSHPLIGSAHLNEAFLSSF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHH
LSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVSGEAPRWIELT
HHHHCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LMDDADTLVISVADSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGG
EECCCCEEEEEEECCCCCCCCCCHHEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
DVWVIDRGTEDGAVFGVKLPGVME
CEEEEECCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MLHALLIITPGGRYVCWSIRTKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMM
CCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQMKTEAEHTALSIGRSVASNPQIREEVALDTQTGANPSAEELADGDIQAVAQAANERTG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCC
ALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETMAWETGTLGASARAKVPIFAP
EEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECC
DSNVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQP
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
EELVTLVQNQTAVIDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGL
HHHHHHHCCCEEEEECCCCCEEEECCCCCEECCCHHHHHHHCCCCCCCCHHHHHCCCCCC
DGVVLHGQHPETVAHNGRILYLDFHPVRRGDQDLGYVVTIRDRTDIIELSERLDSVRTMT
CCEEEECCCCCHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
HALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNGGQSHPLIGSAHLNEAFLSSF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHH
LSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVSGEAPRWIELT
HHHHCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LMDDADTLVISVADSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGG
EECCCCEEEEEEECCCCCCCCCCHHEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
DVWVIDRGTEDGAVFGVKLPGVME
CEEEEECCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]