Click here to switch to the map view.

The map label for this gene is kstD [H]

Identifier: 145294106

GI number: 145294106

Start: 73931

End: 75580

Strand: Reverse

Name: kstD [H]

Synonym: cgR_0064

Alternate gene names: 145294106

Gene position: 75580-73931 (Counterclockwise)

Preceding gene: 145294108

Following gene: 145294105

Centisome position: 2.28

GC content: 54.48

Gene sequence:

>1650_bases
ATGAAAGTTTTAAACGCCGAAAATCTGGCTAACCACTACGATCTCATCGTCGTCGGCTCTGGTGCCGGAGGCCTTACCGC
TGCTGCTACTGCAGCTCACGCTGGACTCTCCGTCCTCGTATTGGAAAAAGCTGAACTGCTCGGTGGTACTTCGGCAGTCT
CCGGTGGAATGCTGTGGGTCGTTGATAACCATTACGCCCGCGAAGCGGGCTTTGCAGATTCCAAGCAAGCAGGCCGCGAG
TACGTCGAAGCGGTTGCCAGGGGGCGCGGGCGTGCAGAGCTTCTCGACGCCGCCCTCCTTCACGGCTCTGACATGCTGGA
ATTCATCGAAGATGAGCTGGATGTAAAGTTCCTCTTCCTAGACAACTTCCCTGACTACCGTCAAGACCTTCCCGGCGCGG
TCCACGGTGGCCGAACCATTGAGCCTCAGCTGTTCAACTACCGTGAAGCGCTCGGTAAGTTAGCCGATTACGTGCGTACC
GATGGCCGGCATCCGTACACGATGCAGGAATATGAAACTTGGGGTGCATTCACCAAGTTCCCGTGGGACGAACTTAATAA
GCGCGTGGAGGATGGTTTCGCAGCCAAAGGCCATGCACTGGTTGCAATGCTGATTGCAGCGTGTATCCGCCTCAATGTCA
CTTTCGCTGTAGATGCGCGCGTCACTAAGTTGCTCGGCGATGCCCAACAGGTAACAGGTATTGAGCTAGAAGAGGGCCAC
ACCATTTCTGCTGGCTCAGTCATGATCGCATCGGGTGGATTTGAATGGGATCGCCAGCTTGCAGATTCATTATTGGCTAC
TCGCTTGTACACAATGTGTTCCCCACCAACTAATACCGGTGATGGCCTGAAAATGGCTCAGCGCATTGGTGCTGCCACCC
GGGGAACCCGCGAAGCATGGTGGGCACCCATGTCGATTACTGGCGGCATGCGCGATGGAGAGCCCATCGGTTCCTTGCTT
CGCTTTGAACGCCAAGGCCCTGGTTCCATCATGGTTAATAGGCATGGGCAGCGTTTTGCCAATGAGGCACAAAACTACAA
TGATCTGGCGCGTTGCCTGCAGTCATGGGATTCTCCGAACAATCAAACATTGAACACTCCGGCCCATGTGGTCTTTGATC
AGTCCTACCTTGACCGCTATGGAATTCTAGATCACCGCTCAGGCCAGCCAACCCCTGATTATCTTATCGAGGCCGCAACT
CTTGAAGAGCTTGCAGCAAAACTCAACGTTCCAGCCTCCAACCTAACTGCTACCGTTGAGCGCTTTAATCAAATGGCGCT
CAAGGGTGTGGATGAAGATTTTGGTCGTGGCGATTCTGAATACGACCGATACTGGGGCGATGCTGACAGCCCGTGGCCAA
ACCCTTCATTAGGTCCACTACAACAAGGACCTTTCTACGCAATGGAAGTGGTGAACGGAGCATTCGGTACCTGTGGTGGC
ATCGCCACTGACGGAAACGCCCAGGTCATCGACGTGGATGGCAACCCCATCTCTGGTTTATTTGCTGCTGGCAATGCCAC
TGAATCTCCCTATGCCTCTGGTTACCCCGGAGCGGGAGCAACTCTTGGACCGCTCATGACCATGGCTTATCAAGCCGGTC
GCACCATTGCTGCCACTGTCCGTATTCCTGCAGAATCGCATGCTTCATGA

Upstream 100 bases:

>100_bases
ACACTCTGTTCCATTGAATGGTTTCTCTCTTTTAATCCAGCTCCACCACCCTCATGATCTAAATCACATACTTAATCAAG
TGCGAACAGGGGCACATTCA

Downstream 100 bases:

>100_bases
TCCGGCATGTTCTTATGATCCGGTGGAAAGATTCCTTCACCGACGATATCCGGAGCAAATGGGTTGACGGTCTTGAAAAG
CTCGAAGCCAATATCCCTGG

Product: hypothetical protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKVLNAENLANHYDLIVVGSGAGGLTAAATAAHAGLSVLVLEKAELLGGTSAVSGGMLWVVDNHYAREAGFADSKQAGRE
YVEAVARGRGRAELLDAALLHGSDMLEFIEDELDVKFLFLDNFPDYRQDLPGAVHGGRTIEPQLFNYREALGKLADYVRT
DGRHPYTMQEYETWGAFTKFPWDELNKRVEDGFAAKGHALVAMLIAACIRLNVTFAVDARVTKLLGDAQQVTGIELEEGH
TISAGSVMIASGGFEWDRQLADSLLATRLYTMCSPPTNTGDGLKMAQRIGAATRGTREAWWAPMSITGGMRDGEPIGSLL
RFERQGPGSIMVNRHGQRFANEAQNYNDLARCLQSWDSPNNQTLNTPAHVVFDQSYLDRYGILDHRSGQPTPDYLIEAAT
LEELAAKLNVPASNLTATVERFNQMALKGVDEDFGRGDSEYDRYWGDADSPWPNPSLGPLQQGPFYAMEVVNGAFGTCGG
IATDGNAQVIDVDGNPISGLFAAGNATESPYASGYPGAGATLGPLMTMAYQAGRTIAATVRIPAESHAS

Sequences:

>Translated_549_residues
MKVLNAENLANHYDLIVVGSGAGGLTAAATAAHAGLSVLVLEKAELLGGTSAVSGGMLWVVDNHYAREAGFADSKQAGRE
YVEAVARGRGRAELLDAALLHGSDMLEFIEDELDVKFLFLDNFPDYRQDLPGAVHGGRTIEPQLFNYREALGKLADYVRT
DGRHPYTMQEYETWGAFTKFPWDELNKRVEDGFAAKGHALVAMLIAACIRLNVTFAVDARVTKLLGDAQQVTGIELEEGH
TISAGSVMIASGGFEWDRQLADSLLATRLYTMCSPPTNTGDGLKMAQRIGAATRGTREAWWAPMSITGGMRDGEPIGSLL
RFERQGPGSIMVNRHGQRFANEAQNYNDLARCLQSWDSPNNQTLNTPAHVVFDQSYLDRYGILDHRSGQPTPDYLIEAAT
LEELAAKLNVPASNLTATVERFNQMALKGVDEDFGRGDSEYDRYWGDADSPWPNPSLGPLQQGPFYAMEVVNGAFGTCGG
IATDGNAQVIDVDGNPISGLFAAGNATESPYASGYPGAGATLGPLMTMAYQAGRTIAATVRIPAESHAS
>Mature_549_residues
MKVLNAENLANHYDLIVVGSGAGGLTAAATAAHAGLSVLVLEKAELLGGTSAVSGGMLWVVDNHYAREAGFADSKQAGRE
YVEAVARGRGRAELLDAALLHGSDMLEFIEDELDVKFLFLDNFPDYRQDLPGAVHGGRTIEPQLFNYREALGKLADYVRT
DGRHPYTMQEYETWGAFTKFPWDELNKRVEDGFAAKGHALVAMLIAACIRLNVTFAVDARVTKLLGDAQQVTGIELEEGH
TISAGSVMIASGGFEWDRQLADSLLATRLYTMCSPPTNTGDGLKMAQRIGAATRGTREAWWAPMSITGGMRDGEPIGSLL
RFERQGPGSIMVNRHGQRFANEAQNYNDLARCLQSWDSPNNQTLNTPAHVVFDQSYLDRYGILDHRSGQPTPDYLIEAAT
LEELAAKLNVPASNLTATVERFNQMALKGVDEDFGRGDSEYDRYWGDADSPWPNPSLGPLQQGPFYAMEVVNGAFGTCGG
IATDGNAQVIDVDGNPISGLFAAGNATESPYASGYPGAGATLGPLMTMAYQAGRTIAATVRIPAESHAS

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

Organism=Caenorhabditis elegans, GI71986328, Length=336, Percent_Identity=25.297619047619, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 59108; Mature: 59108

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00175 PG_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLNAENLANHYDLIVVGSGAGGLTAAATAAHAGLSVLVLEKAELLGGTSAVSGGMLWV
CCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCEEEE
VDNHYAREAGFADSKQAGREYVEAVARGRGRAELLDAALLHGSDMLEFIEDELDVKFLFL
ECCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEEE
DNFPDYRQDLPGAVHGGRTIEPQLFNYREALGKLADYVRTDGRHPYTMQEYETWGAFTKF
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
PWDELNKRVEDGFAAKGHALVAMLIAACIRLNVTFAVDARVTKLLGDAQQVTGIELEEGH
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEEHHHHHHHHCCHHHHCCEEECCCC
TISAGSVMIASGGFEWDRQLADSLLATRLYTMCSPPTNTGDGLKMAQRIGAATRGTREAW
EEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHCCCCHHHH
WAPMSITGGMRDGEPIGSLLRFERQGPGSIMVNRHGQRFANEAQNYNDLARCLQSWDSPN
CCCEEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHCCCC
NQTLNTPAHVVFDQSYLDRYGILDHRSGQPTPDYLIEAATLEELAAKLNVPASNLTATVE
CCEECCCCEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RFNQMALKGVDEDFGRGDSEYDRYWGDADSPWPNPSLGPLQQGPFYAMEVVNGAFGTCGG
HHHHHHHHCCCHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
IATDGNAQVIDVDGNPISGLFAAGNATESPYASGYPGAGATLGPLMTMAYQAGRTIAATV
EEECCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
RIPAESHAS
EECCCCCCC
>Mature Secondary Structure
MKVLNAENLANHYDLIVVGSGAGGLTAAATAAHAGLSVLVLEKAELLGGTSAVSGGMLWV
CCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCEEEE
VDNHYAREAGFADSKQAGREYVEAVARGRGRAELLDAALLHGSDMLEFIEDELDVKFLFL
ECCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEEE
DNFPDYRQDLPGAVHGGRTIEPQLFNYREALGKLADYVRTDGRHPYTMQEYETWGAFTKF
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
PWDELNKRVEDGFAAKGHALVAMLIAACIRLNVTFAVDARVTKLLGDAQQVTGIELEEGH
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEEHHHHHHHHCCHHHHCCEEECCCC
TISAGSVMIASGGFEWDRQLADSLLATRLYTMCSPPTNTGDGLKMAQRIGAATRGTREAW
EEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHCCCCHHHH
WAPMSITGGMRDGEPIGSLLRFERQGPGSIMVNRHGQRFANEAQNYNDLARCLQSWDSPN
CCCEEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHCCCC
NQTLNTPAHVVFDQSYLDRYGILDHRSGQPTPDYLIEAATLEELAAKLNVPASNLTATVE
CCEECCCCEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RFNQMALKGVDEDFGRGDSEYDRYWGDADSPWPNPSLGPLQQGPFYAMEVVNGAFGTCGG
HHHHHHHHCCCHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
IATDGNAQVIDVDGNPISGLFAAGNATESPYASGYPGAGATLGPLMTMAYQAGRTIAATV
EEECCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
RIPAESHAS
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]