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The map label for this gene is gyrB [H]

Identifier: 145294048

GI number: 145294048

Start: 5435

End: 7489

Strand: Direct

Name: gyrB [H]

Synonym: cgR_0006

Alternate gene names: 145294048

Gene position: 5435-7489 (Clockwise)

Preceding gene: 145294047

Following gene: 145294049

Centisome position: 0.16

GC content: 55.96

Gene sequence:

>2055_bases
GTGGCAAACACTGAACACAATTATGACGCTTCATCGATCACCATCCTTGAAGGTCTTGAGGCGGTACGTAAGCGCCCGGG
CATGTACATCGGTTCAACTGGACCGCGTGGACTGCACCACCTGATTTGGGAAGTCGTTGACAACTCAGTGGATGAGGCCA
TGGCTGGCCACGCCACCAAGGTTGAAGTGACCCTTCTGGAAGATGGTGGCGTTCAAGTTGTCGATGACGGTCGAGGAATT
CCCGTCGATATGCACCCATCCGGTGCACCAACCGTGCAGGTTGTTATGACCCAGCTGCACGCCGGCGGTAAGTTCGACTC
CGATTCTTACGCCGTTTCCGGTGGTCTGCACGGTGTTGGTATTTCTGTGGTGAACGCCCTGTCCACCCGCGTGGAAGCCG
ACATCAAGTTGCACGGCAAGCACTGGTACCAAAACTTTGAAAAGTCTGTTCCAGACGAGTTGATCGAAGGCGGCAACGCT
CGTGGCACCGGTACCACCATTCGTTTTTGGCCAGACGCTGAAATTTTCGAAACCACCGAGTTTGATTTCGAAACGATTTC
TCGACGTCTGCAGGAAATGGCATTCCTTAACAAGGGTCTGACCATCACCTTGACGGACAACCGCGCTACCGACGAGGAAC
TCGAGCTCGAAGCACTCGCTGAGCAGGGCGAAACCGCAACGGAACTATCCCTCGATGAGATCGACAACGAAACCGAACTC
GTTGAAGAGACCACCGATGCTCCAAAGAAGCCAAAAAAGCGTGAGAAGAAGAAAATCTTCCACTACCCCAATGGCCTCGA
GGACTACGTTCAGTACCTCAACCGCAGCAAGACCAACATCCACCCTTCAATCGTGTCATTCGAGGCAAAGGGAGATGACC
ACGAGGTTGAGGTGGCAATGCAGTGGAACTCCTCCTACAAGGAATCCGTCCACACCTTCGCCAACACCATTAACACCCGC
GAAGGCGGCACCCACGAGGAAGGTTTCCGCTCTGCGCTGACCTCCCTGATGAACCGCTACGCACGTGAGCACAAGCTTCT
GAAAGAAAAGGAAGCAAACCTTACCGGTGACGACTGTCGTGAAGGCCTGTCCGCGGTTATTTCCGTGCGCGTTGGTGACC
CACAGTTCGAAGGCCAGACCAAAACCAAGCTGGGCAACACGGAGATCAAATCCTTCGTGCAGCGCATGGCCAACGAGCAC
ATCGGCCACTGGTTGGAAGCAAACCCTGCTGAAGCCAAGGTCATCATCAACAAGGCTGTCGGTTCCGCGCAGGCACGCCT
TGCTGCTCGAAAAGCCCGTGACCTGGTCCGACGCAAGTCAGCAACCGATCTGGGTGGACTGCCCGGTAAGCTTGCCGACT
GCCGTTCCAAGGATCCAGAAAAGTCCGAACTTTACATCGTGGAGGGCGACTCCGCAGGTGGTTCTGCGAAGTCCGGCCGT
GACTCCATGTTCCAGGCAATCCTTCCACTGCGAGGCAAGATCCTCAACGTGGAAAAGGCCCGCCTAGACAAGGTTCTGAA
GAACGCCGAAGTCCAAGCGATCATCACCGCACTGGGTACCGGCATCCACGACGAGTTCGACATCAACAAGCTGCGCTACC
ACAAGATCGTGCTGATGGCCGACGCCGACGTTGACGGCCAGCACATCGCAACGCTGCTGCTCACCCTGCTTTTCCGCTTC
ATGCCAGACCTCGTCGCCGAAGGCCACGTCTACTTGGCGCAGCCACCTTTGTACAAACTGAAGTGGCAGCGCGGCGAGCC
AGGATTCGCATACTCCGATGAGGAGCGCGATGAGCAGCTCAACGAAGGCCTTGCCGCTGGACGCAAGATCAACAAGGATG
ATGGCATCCAGCGCTACAAGGGTCTCGGCGAGATGAACGCCAGCGAGCTGTGGGAAACCACCATGGACCCAACTGTTCGT
ATTCTGCGCCGCGTGGACATCACCGATGCTCAGCGTGCTGATGAACTGTTCTCCATCTTGATGGGTGACGACGTTGTGGC
TCGCCGCAGCTTCATCACCCGAAATGCCAAGGATGTTCGTTTCCTCGATATCTAA

Upstream 100 bases:

>100_bases
TTTCGCCGTTTCTCGCGTTGTGCGTGGTACTACGTGGGGACCTAAGCGTGTAAGATAGAAACGTCTGTATCGGATAAGTA
GCGAGGAGTGTTCGTTAAAA

Downstream 100 bases:

>100_bases
AGCGCCTTACTTAACCCGCCCCTGGAATTCTGGGGGCGGGTTTTGTGATTTTTAGGGTCAGCACTTTATAAATGCAGGCT
TCTATGGCTTCAAGTTGGCC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MANTEHNYDASSITILEGLEAVRKRPGMYIGSTGPRGLHHLIWEVVDNSVDEAMAGHATKVEVTLLEDGGVQVVDDGRGI
PVDMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRVEADIKLHGKHWYQNFEKSVPDELIEGGNA
RGTGTTIRFWPDAEIFETTEFDFETISRRLQEMAFLNKGLTITLTDNRATDEELELEALAEQGETATELSLDEIDNETEL
VEETTDAPKKPKKREKKKIFHYPNGLEDYVQYLNRSKTNIHPSIVSFEAKGDDHEVEVAMQWNSSYKESVHTFANTINTR
EGGTHEEGFRSALTSLMNRYAREHKLLKEKEANLTGDDCREGLSAVISVRVGDPQFEGQTKTKLGNTEIKSFVQRMANEH
IGHWLEANPAEAKVIINKAVGSAQARLAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKSGR
DSMFQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDINKLRYHKIVLMADADVDGQHIATLLLTLLFRF
MPDLVAEGHVYLAQPPLYKLKWQRGEPGFAYSDEERDEQLNEGLAAGRKINKDDGIQRYKGLGEMNASELWETTMDPTVR
ILRRVDITDAQRADELFSILMGDDVVARRSFITRNAKDVRFLDI

Sequences:

>Translated_684_residues
MANTEHNYDASSITILEGLEAVRKRPGMYIGSTGPRGLHHLIWEVVDNSVDEAMAGHATKVEVTLLEDGGVQVVDDGRGI
PVDMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRVEADIKLHGKHWYQNFEKSVPDELIEGGNA
RGTGTTIRFWPDAEIFETTEFDFETISRRLQEMAFLNKGLTITLTDNRATDEELELEALAEQGETATELSLDEIDNETEL
VEETTDAPKKPKKREKKKIFHYPNGLEDYVQYLNRSKTNIHPSIVSFEAKGDDHEVEVAMQWNSSYKESVHTFANTINTR
EGGTHEEGFRSALTSLMNRYAREHKLLKEKEANLTGDDCREGLSAVISVRVGDPQFEGQTKTKLGNTEIKSFVQRMANEH
IGHWLEANPAEAKVIINKAVGSAQARLAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKSGR
DSMFQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDINKLRYHKIVLMADADVDGQHIATLLLTLLFRF
MPDLVAEGHVYLAQPPLYKLKWQRGEPGFAYSDEERDEQLNEGLAAGRKINKDDGIQRYKGLGEMNASELWETTMDPTVR
ILRRVDITDAQRADELFSILMGDDVVARRSFITRNAKDVRFLDI
>Mature_683_residues
ANTEHNYDASSITILEGLEAVRKRPGMYIGSTGPRGLHHLIWEVVDNSVDEAMAGHATKVEVTLLEDGGVQVVDDGRGIP
VDMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRVEADIKLHGKHWYQNFEKSVPDELIEGGNAR
GTGTTIRFWPDAEIFETTEFDFETISRRLQEMAFLNKGLTITLTDNRATDEELELEALAEQGETATELSLDEIDNETELV
EETTDAPKKPKKREKKKIFHYPNGLEDYVQYLNRSKTNIHPSIVSFEAKGDDHEVEVAMQWNSSYKESVHTFANTINTRE
GGTHEEGFRSALTSLMNRYAREHKLLKEKEANLTGDDCREGLSAVISVRVGDPQFEGQTKTKLGNTEIKSFVQRMANEHI
GHWLEANPAEAKVIINKAVGSAQARLAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKSGRD
SMFQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDINKLRYHKIVLMADADVDGQHIATLLLTLLFRFM
PDLVAEGHVYLAQPPLYKLKWQRGEPGFAYSDEERDEQLNEGLAAGRKINKDDGIQRYKGLGEMNASELWETTMDPTVRI
LRRVDITDAQRADELFSILMGDDVVARRSFITRNAKDVRFLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=677, Percent_Identity=23.1905465288035, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI19913408, Length=343, Percent_Identity=27.6967930029155, Blast_Score=94, Evalue=6e-19,
Organism=Escherichia coli, GI48994957, Length=598, Percent_Identity=50, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1789408, Length=666, Percent_Identity=39.1891891891892, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17535065, Length=386, Percent_Identity=27.2020725388601, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI212645845, Length=397, Percent_Identity=26.448362720403, Blast_Score=102, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=208, Percent_Identity=31.7307692307692, Blast_Score=79, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=696, Percent_Identity=25, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17136538, Length=350, Percent_Identity=27.7142857142857, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 76004; Mature: 75873

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTEHNYDASSITILEGLEAVRKRPGMYIGSTGPRGLHHLIWEVVDNSVDEAMAGHATK
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHCCCCEE
VEVTLLEDGGVQVVDDGRGIPVDMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVG
EEEEEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
ISVVNALSTRVEADIKLHGKHWYQNFEKSVPDELIEGGNARGTGTTIRFWPDAEIFETTE
HHHHHHHHHHHCCCEEECCHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCEEECCC
FDFETISRRLQEMAFLNKGLTITLTDNRATDEELELEALAEQGETATELSLDEIDNETEL
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHCCCHHHCCCHHHH
VEETTDAPKKPKKREKKKIFHYPNGLEDYVQYLNRSKTNIHPSIVSFEAKGDDHEVEVAM
HHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEE
QWNSSYKESVHTFANTINTREGGTHEEGFRSALTSLMNRYAREHKLLKEKEANLTGDDCR
ECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EGLSAVISVRVGDPQFEGQTKTKLGNTEIKSFVQRMANEHIGHWLEANPAEAKVIINKAV
HHHHHEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHH
GSAQARLAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKSGR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCH
DSMFQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDINKLRYHKIVLMA
HHHHHHHHHHCCCEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEE
DADVDGQHIATLLLTLLFRFMPDLVAEGHVYLAQPPLYKLKWQRGEPGFAYSDEERDEQL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHH
NEGLAAGRKINKDDGIQRYKGLGEMNASELWETTMDPTVRILRRVDITDAQRADELFSIL
HHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHH
MGDDVVARRSFITRNAKDVRFLDI
HCCHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
ANTEHNYDASSITILEGLEAVRKRPGMYIGSTGPRGLHHLIWEVVDNSVDEAMAGHATK
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHCCCCEE
VEVTLLEDGGVQVVDDGRGIPVDMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVG
EEEEEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
ISVVNALSTRVEADIKLHGKHWYQNFEKSVPDELIEGGNARGTGTTIRFWPDAEIFETTE
HHHHHHHHHHHCCCEEECCHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCEEECCC
FDFETISRRLQEMAFLNKGLTITLTDNRATDEELELEALAEQGETATELSLDEIDNETEL
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHCCCHHHCCCHHHH
VEETTDAPKKPKKREKKKIFHYPNGLEDYVQYLNRSKTNIHPSIVSFEAKGDDHEVEVAM
HHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEE
QWNSSYKESVHTFANTINTREGGTHEEGFRSALTSLMNRYAREHKLLKEKEANLTGDDCR
ECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EGLSAVISVRVGDPQFEGQTKTKLGNTEIKSFVQRMANEHIGHWLEANPAEAKVIINKAV
HHHHHEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHH
GSAQARLAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKSGR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCH
DSMFQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDINKLRYHKIVLMA
HHHHHHHHHHCCCEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEE
DADVDGQHIATLLLTLLFRFMPDLVAEGHVYLAQPPLYKLKWQRGEPGFAYSDEERDEQL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHH
NEGLAAGRKINKDDGIQRYKGLGEMNASELWETTMDPTVRILRRVDITDAQRADELFSIL
HHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHH
MGDDVVARRSFITRNAKDVRFLDI
HCCHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7981652 [H]