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The map label for this gene is bfmBB [H]

Identifier: 162330296

GI number: 162330296

Start: 2426461

End: 2427786

Strand: Reverse

Name: bfmBB [H]

Synonym: GTNG_2305

Alternate gene names: 162330296

Gene position: 2427786-2426461 (Counterclockwise)

Preceding gene: 138895947

Following gene: 138895945

Centisome position: 68.38

GC content: 55.81

Gene sequence:

>1326_bases
GTGGCCATCGAACAATTGACGATGCCTCAGCTCGGGGAAAGTGTTACGGAAGGCACAATCAGTAAATGGCTCGTTTCCCC
GGGCGACAAAGTGAACAAATACGATCCGGTTGCCGAAGTGATGACTGATAAAGTGAGCGCGGAAATTCCGTCGTCATTTG
CCGGCGTCATCCGCGAGTTGATCGCCAAAGAAGGAGAAACGTTGCCGGTCGGCGCCCCGATTTGCACAATCGAAGTCGAA
GGCGCTGCACCAGCTCCGGAAGCGAAGCCGACGGAAGAGACAGCAGGAACGAAAACGGAAAATGAAAACAAAGCTCCCGC
GGCCAAACAGGCTGGCAAGGCCAACAACGGCCGTTACTCCCCGGCTGTGCTCCGTTTGGCCCAAGAACATGGAATTGACC
TTGAACAAGTCAAAGGCACTGGTCTTGGCGGGCGAGTGACGCGCAAAGATTTGCTTAAGTTGATCGAGTCCGGCCAAATT
CCGAAAGCGGAAGCGGCAGAACAGGCGGCACCAAAGGTCGAGACGACAGCTCCGGACGTCGCTCCATCTCAGCCGGCTGC
TGCCGCACAAACCGCGCCGGTGACGACGTCGACGAAGCCAGCGGTGCCAACGATCGAAGTAGGAGCGGGCGATATCGAAA
TTCCGGTCACCCCGGTGCGCAAAGCGATCGCCGCGAACATGCTCCGAAGCAAGCACGAAGCGCCGCACGCATGGACGATG
GTCGAAGTCGACGTGACGAACCTTGTCGCTTATCGCGATGCCATCAAAGACGAATTCAAGCGGCGTGAAGGCTTCAACTT
GACGTACTTTGCCTTTTTCGTCAAAGCGGTCGCTCAAGCGTTGAAAGAATTCCCGCAGCTGAATTCGGTGTGGGCCGGCG
ATAAAATCATCCAGCGCAAGGACATCAACATCTCGATTGCCGTCGCGACCGATGATGCGTTGTTCGTTCCGGTCATCAAG
CACGCCGATGAGAAAACGATCAAGGGGATTGCCCGCGAAATCGCCGAACTGGCAGCGAAAACGCGCGCCGGCAAACTCCG
TCCGGAAGATATGCAAGGCGGCACGTTCACCGTCAACAACACTGGCGCGTTTGGCTCAGTGCAGTCAATGGGTATTATCA
ACTACCCGCAAGCTGCCATTTTGCAAGTGGAAACAATCGTCAAACGCCCGGTCGTGAAAGACGGCATGATTGCCATCCGC
GACATGGTCAACTTGTGCCTGTCGCTTGACCACCGCGTCCTAGACGGTCTCGTTTGCGGCCGCTTCCTCGCCCGTGTCAA
AGCGATTTTGGAAAACATGAACAAAGACAATACACCGGTTTATTAA

Upstream 100 bases:

>100_bases
AGGAGTCCCATCCCGTGAAGAGGTTCCCCCCCTGCCCAAAGCGGATGTGGCGGGGGATGTCACGGCAAAGACATTGCAAC
AGAGATAGGAGGGTATCGAT

Downstream 100 bases:

>100_bases
CGGTCCCCCCATCGCCATCGGTGGCGATGGGGGTTTTTGCATGGTACGATTTCAAACTTCTTTCACAAATGTCATGTATA
ATGGAAACAGAAGTCCATGT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAIEQLTMPQLGESVTEGTISKWLVSPGDKVNKYDPVAEVMTDKVSAEIPSSFAGVIRELIAKEGETLPVGAPICTIEVE
GAAPAPEAKPTEETAGTKTENENKAPAAKQAGKANNGRYSPAVLRLAQEHGIDLEQVKGTGLGGRVTRKDLLKLIESGQI
PKAEAAEQAAPKVETTAPDVAPSQPAAAAQTAPVTTSTKPAVPTIEVGAGDIEIPVTPVRKAIAANMLRSKHEAPHAWTM
VEVDVTNLVAYRDAIKDEFKRREGFNLTYFAFFVKAVAQALKEFPQLNSVWAGDKIIQRKDINISIAVATDDALFVPVIK
HADEKTIKGIAREIAELAAKTRAGKLRPEDMQGGTFTVNNTGAFGSVQSMGIINYPQAAILQVETIVKRPVVKDGMIAIR
DMVNLCLSLDHRVLDGLVCGRFLARVKAILENMNKDNTPVY

Sequences:

>Translated_441_residues
MAIEQLTMPQLGESVTEGTISKWLVSPGDKVNKYDPVAEVMTDKVSAEIPSSFAGVIRELIAKEGETLPVGAPICTIEVE
GAAPAPEAKPTEETAGTKTENENKAPAAKQAGKANNGRYSPAVLRLAQEHGIDLEQVKGTGLGGRVTRKDLLKLIESGQI
PKAEAAEQAAPKVETTAPDVAPSQPAAAAQTAPVTTSTKPAVPTIEVGAGDIEIPVTPVRKAIAANMLRSKHEAPHAWTM
VEVDVTNLVAYRDAIKDEFKRREGFNLTYFAFFVKAVAQALKEFPQLNSVWAGDKIIQRKDINISIAVATDDALFVPVIK
HADEKTIKGIAREIAELAAKTRAGKLRPEDMQGGTFTVNNTGAFGSVQSMGIINYPQAAILQVETIVKRPVVKDGMIAIR
DMVNLCLSLDHRVLDGLVCGRFLARVKAILENMNKDNTPVY
>Mature_440_residues
AIEQLTMPQLGESVTEGTISKWLVSPGDKVNKYDPVAEVMTDKVSAEIPSSFAGVIRELIAKEGETLPVGAPICTIEVEG
AAPAPEAKPTEETAGTKTENENKAPAAKQAGKANNGRYSPAVLRLAQEHGIDLEQVKGTGLGGRVTRKDLLKLIESGQIP
KAEAAEQAAPKVETTAPDVAPSQPAAAAQTAPVTTSTKPAVPTIEVGAGDIEIPVTPVRKAIAANMLRSKHEAPHAWTMV
EVDVTNLVAYRDAIKDEFKRREGFNLTYFAFFVKAVAQALKEFPQLNSVWAGDKIIQRKDINISIAVATDDALFVPVIKH
ADEKTIKGIAREIAELAAKTRAGKLRPEDMQGGTFTVNNTGAFGSVQSMGIINYPQAAILQVETIVKRPVVKDGMIAIRD
MVNLCLSLDHRVLDGLVCGRFLARVKAILENMNKDNTPVY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=31.9474835886214, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=31.8681318681319, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI31711992, Length=453, Percent_Identity=31.3465783664459, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=32.1759259259259, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=36.5853658536585, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=40.7643312101911, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786946, Length=435, Percent_Identity=32.4137931034483, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786305, Length=446, Percent_Identity=32.9596412556054, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=439, Percent_Identity=30.9794988610478, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI25146366, Length=428, Percent_Identity=31.5420560747664, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=462, Percent_Identity=29.004329004329, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=28.125, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=433, Percent_Identity=30.715935334873, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=28.2608695652174, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=30.1369863013699, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI20129315, Length=223, Percent_Identity=36.322869955157, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=36.322869955157, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24645909, Length=220, Percent_Identity=36.8181818181818, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47270; Mature: 47139

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEQLTMPQLGESVTEGTISKWLVSPGDKVNKYDPVAEVMTDKVSAEIPSSFAGVIREL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
IAKEGETLPVGAPICTIEVEGAAPAPEAKPTEETAGTKTENENKAPAAKQAGKANNGRYS
HHCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHCCCCCCCCCC
PAVLRLAQEHGIDLEQVKGTGLGGRVTRKDLLKLIESGQIPKAEAAEQAAPKVETTAPDV
HHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCC
APSQPAAAAQTAPVTTSTKPAVPTIEVGAGDIEIPVTPVRKAIAANMLRSKHEAPHAWTM
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCCEEEE
VEVDVTNLVAYRDAIKDEFKRREGFNLTYFAFFVKAVAQALKEFPQLNSVWAGDKIIQRK
EEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEEE
DINISIAVATDDALFVPVIKHADEKTIKGIAREIAELAAKTRAGKLRPEDMQGGTFTVNN
CCEEEEEEECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
TGAFGSVQSMGIINYPQAAILQVETIVKRPVVKDGMIAIRDMVNLCLSLDHRVLDGLVCG
CCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLARVKAILENMNKDNTPVY
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AIEQLTMPQLGESVTEGTISKWLVSPGDKVNKYDPVAEVMTDKVSAEIPSSFAGVIREL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
IAKEGETLPVGAPICTIEVEGAAPAPEAKPTEETAGTKTENENKAPAAKQAGKANNGRYS
HHCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHCCCCCCCCCC
PAVLRLAQEHGIDLEQVKGTGLGGRVTRKDLLKLIESGQIPKAEAAEQAAPKVETTAPDV
HHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCC
APSQPAAAAQTAPVTTSTKPAVPTIEVGAGDIEIPVTPVRKAIAANMLRSKHEAPHAWTM
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCCEEEE
VEVDVTNLVAYRDAIKDEFKRREGFNLTYFAFFVKAVAQALKEFPQLNSVWAGDKIIQRK
EEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEEE
DINISIAVATDDALFVPVIKHADEKTIKGIAREIAELAAKTRAGKLRPEDMQGGTFTVNN
CCEEEEEEECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
TGAFGSVQSMGIINYPQAAILQVETIVKRPVVKDGMIAIRDMVNLCLSLDHRVLDGLVCG
CCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLARVKAILENMNKDNTPVY
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]