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The map label for this gene is trmE [H]

Identifier: 138897067

GI number: 138897067

Start: 3546317

End: 3547705

Strand: Reverse

Name: trmE [H]

Synonym: GTNG_3440

Alternate gene names: 138897067

Gene position: 3547705-3546317 (Counterclockwise)

Preceding gene: 138897068

Following gene: 138897066

Centisome position: 99.93

GC content: 51.12

Gene sequence:

>1389_bases
ATGACAGAATTTGATACAATTGCCGCTATTTCGACGCCGATGGGAGAAGGGGCGATCGCCATTGTTCGGCTGAGCGGGGA
TGAGGCGGTAGAGATCGCCGATCGGCTTTTCCGCAGCCCAACTGGAAAGCAGCTGAAAGACGTGCCGTCACACACGATTC
ATTATGGGCATATTGTTGACCCAAAAAGCGGGCGGATCGTTGAGGAAGTGATGGTATCGGTCATGCGGGCGCCGAAAACA
TTTACGCGTGAAGATGTAGTAGAGATTAATTGCCATGGCGGGTTCGTTTCCGTCAATCGGGTGTTGCAGCTTGTGCTCGC
GAACGGGGCGCGTCTCGCCGAGCCGGGGGAGTTTACGAAGCGAGCGTTTTTAAACGGGCGCATCGACTTATCCCAGGCTG
AAGCGGTCATTGACCTCATTCGCGCCAAAACGGATCGAGCGATGAACGTCGCCTTGCAGCAAATGGAGGGGCGCTTGTCG
AAATTGATCCGCGAATTGCGGCAGACGATTTTAGAAACGTTGGCGCACGTTGAAGTCAACATCGACTATCCGGAATACGA
TGATGTCGAGGAGATGACGCCGCGTCTACTAAAAGAGAAAGCAGAATACGTGCGTGGGCAAATTGAAAAACTGCTTTCGA
CCGCTACCCAAGGGAAAATTTTGCGCGAAGGTTTGGCGACGGTTATTATCGGGCGGCCGAACGTCGGCAAGTCCTCACTG
TTAAATGCGCTTGCTCACGAAAACCGAGCGATCGTCACTGATATTCCGGGAACGACGCGCGATGTCATCGAAGAGTATGT
CAACGTTCGCGGTGTGCCGCTCCGCTTGATTGATACGGCCGGCATCCGTGAAACAGAAGATATTGTTGAACGGATTGGCG
TCGAGCGCTCACGGCAAATGTTGAAGAAGGCCGATTTAATTTTGCTTGTTTTGAACTATCATGAACCGCTGACAGAAGAG
GATGAGCGGCTGTTTGCAATGACAGAAGGAATGGATGTTATTGTTATCGTCAATAAAACGGATTTGCCTCAGAACATTGA
TATAGAGCGGGTCAAAGAGCTTGCGGCTGGCCGGCCGATTGTGGCAACATCACTATTGTGTGAACGAGGAATTGATGAAC
TGGAAAAAGCGATTGCTGACTTATTTTTTGGTGGTGAACTCGAAGCCGGCGATTTGACTTATGTCTCGAATTCCCGGCAT
ATCGCGTTGCTCGAACAGGCGAAAAAGGCGATCGAAGATGCGCTGTCCGGCATTGATGTTGGCATGCCGGTCGATCTCGT
TCAAATCGATTTGCGCCGCGCGTGGGAGCTGCTTGGCGAAATCGTTGGCGATACAGTGCATGAAAGCCTGATTGATCAGC
TGTTTGCCCAATTTTGTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
GGATAAGTTAAAATTGAACTAAACAAAAATACACAAGGTGAATACGGCCAAAAAAAGTTGTGGATATAGATGGAACGGAG
AAAAGCGAGGTGAACAAGCC

Downstream 100 bases:

>100_bases
ACAAGGAGGAACCGAAATGGAGTATCACGGAGGATCGTATGACGTCATCGTCATCGGTGCTGGTCATGCCGGCTGCGAGG
CGGCTCTGGCGTCTGCGCGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTEFDTIAAISTPMGEGAIAIVRLSGDEAVEIADRLFRSPTGKQLKDVPSHTIHYGHIVDPKSGRIVEEVMVSVMRAPKT
FTREDVVEINCHGGFVSVNRVLQLVLANGARLAEPGEFTKRAFLNGRIDLSQAEAVIDLIRAKTDRAMNVALQQMEGRLS
KLIRELRQTILETLAHVEVNIDYPEYDDVEEMTPRLLKEKAEYVRGQIEKLLSTATQGKILREGLATVIIGRPNVGKSSL
LNALAHENRAIVTDIPGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVERIGVERSRQMLKKADLILLVLNYHEPLTEE
DERLFAMTEGMDVIVIVNKTDLPQNIDIERVKELAAGRPIVATSLLCERGIDELEKAIADLFFGGELEAGDLTYVSNSRH
IALLEQAKKAIEDALSGIDVGMPVDLVQIDLRRAWELLGEIVGDTVHESLIDQLFAQFCLGK

Sequences:

>Translated_462_residues
MTEFDTIAAISTPMGEGAIAIVRLSGDEAVEIADRLFRSPTGKQLKDVPSHTIHYGHIVDPKSGRIVEEVMVSVMRAPKT
FTREDVVEINCHGGFVSVNRVLQLVLANGARLAEPGEFTKRAFLNGRIDLSQAEAVIDLIRAKTDRAMNVALQQMEGRLS
KLIRELRQTILETLAHVEVNIDYPEYDDVEEMTPRLLKEKAEYVRGQIEKLLSTATQGKILREGLATVIIGRPNVGKSSL
LNALAHENRAIVTDIPGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVERIGVERSRQMLKKADLILLVLNYHEPLTEE
DERLFAMTEGMDVIVIVNKTDLPQNIDIERVKELAAGRPIVATSLLCERGIDELEKAIADLFFGGELEAGDLTYVSNSRH
IALLEQAKKAIEDALSGIDVGMPVDLVQIDLRRAWELLGEIVGDTVHESLIDQLFAQFCLGK
>Mature_461_residues
TEFDTIAAISTPMGEGAIAIVRLSGDEAVEIADRLFRSPTGKQLKDVPSHTIHYGHIVDPKSGRIVEEVMVSVMRAPKTF
TREDVVEINCHGGFVSVNRVLQLVLANGARLAEPGEFTKRAFLNGRIDLSQAEAVIDLIRAKTDRAMNVALQQMEGRLSK
LIRELRQTILETLAHVEVNIDYPEYDDVEEMTPRLLKEKAEYVRGQIEKLLSTATQGKILREGLATVIIGRPNVGKSSLL
NALAHENRAIVTDIPGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVERIGVERSRQMLKKADLILLVLNYHEPLTEED
ERLFAMTEGMDVIVIVNKTDLPQNIDIERVKELAAGRPIVATSLLCERGIDELEKAIADLFFGGELEAGDLTYVSNSRHI
ALLEQAKKAIEDALSGIDVGMPVDLVQIDLRRAWELLGEIVGDTVHESLIDQLFAQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=476, Percent_Identity=33.6134453781513, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI193082993, Length=465, Percent_Identity=33.3333333333333, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=32.2834645669291, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI2367268, Length=471, Percent_Identity=33.9702760084926, Blast_Score=257, Evalue=9e-70,
Organism=Escherichia coli, GI87082120, Length=131, Percent_Identity=40.4580152671756, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=30.6034482758621, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=31.9105691056911, Blast_Score=220, Evalue=4e-58,
Organism=Drosophila melanogaster, GI45550813, Length=482, Percent_Identity=31.1203319502075, Blast_Score=202, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51265; Mature: 51134

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFDTIAAISTPMGEGAIAIVRLSGDEAVEIADRLFRSPTGKQLKDVPSHTIHYGHIVD
CCCCHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCCEEECEEEC
PKSGRIVEEVMVSVMRAPKTFTREDVVEINCHGGFVSVNRVLQLVLANGARLAEPGEFTK
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRIDLSQAEAVIDLIRAKTDRAMNVALQQMEGRLSKLIRELRQTILETLAHVEVN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTPRLLKEKAEYVRGQIEKLLSTATQGKILREGLATVIIGRPNVGKSSL
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
LNALAHENRAIVTDIPGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVERIGVERSRQM
HHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHH
LKKADLILLVLNYHEPLTEEDERLFAMTEGMDVIVIVNKTDLPQNIDIERVKELAAGRPI
HHHCCEEEEEEECCCCCCCCHHHEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHCCCCH
VATSLLCERGIDELEKAIADLFFGGELEAGDLTYVSNSRHIALLEQAKKAIEDALSGIDV
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
GMPVDLVQIDLRRAWELLGEIVGDTVHESLIDQLFAQFCLGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEFDTIAAISTPMGEGAIAIVRLSGDEAVEIADRLFRSPTGKQLKDVPSHTIHYGHIVD
CCCHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCCEEECEEEC
PKSGRIVEEVMVSVMRAPKTFTREDVVEINCHGGFVSVNRVLQLVLANGARLAEPGEFTK
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRIDLSQAEAVIDLIRAKTDRAMNVALQQMEGRLSKLIRELRQTILETLAHVEVN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTPRLLKEKAEYVRGQIEKLLSTATQGKILREGLATVIIGRPNVGKSSL
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
LNALAHENRAIVTDIPGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVERIGVERSRQM
HHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHH
LKKADLILLVLNYHEPLTEEDERLFAMTEGMDVIVIVNKTDLPQNIDIERVKELAAGRPI
HHHCCEEEEEEECCCCCCCCHHHEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHCCCCH
VATSLLCERGIDELEKAIADLFFGGELEAGDLTYVSNSRHIALLEQAKKAIEDALSGIDV
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
GMPVDLVQIDLRRAWELLGEIVGDTVHESLIDQLFAQFCLGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA