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The map label for this gene is gidB

Identifier: 138897065

GI number: 138897065

Start: 3543671

End: 3544387

Strand: Reverse

Name: gidB

Synonym: GTNG_3438

Alternate gene names: 138897065

Gene position: 3544387-3543671 (Counterclockwise)

Preceding gene: 138897066

Following gene: 138897064

Centisome position: 99.83

GC content: 52.16

Gene sequence:

>717_bases
ATGGAGGCGACACAATTTCAAGCCATGCTCGAAGAGAGGGGAATTTCCCTCTCTTCCCAGGCGCTTGCACAGTTTGAGCG
CTATTATGAACTGCTCGTTGAATGGAACGAGAAGATGAATTTGACCGCCATTACGGACAAGCCCGGTGTATATGTGAAAC
ATTTTTTTGATTCGGTTTCTCCGGCGTTTTATTACGACTTTTCCGAGCCGTTCTCGCTTTGCGACGTTGGTGCTGGGGCA
GGATTCCCGAGCATCCCGCTTAAAATTTGCTTTCCGCACTTGCGCGTTTCGATTGTCGATTCGCTGCAAAAACGCATCCG
CTTTTTACAGCATCTTGTTGGGGAACTAGGGCTTAAAGATATCGCCCTTTACCACGATCGTGCCGAGACGTTTGCTCGCC
AAAAGGGGATGCGCGAATCGTTCGACGTCGTTACGGCTAGGGCAGTGGCGCGTATGCCGGTGCTTGCCGAGCTTTGCCTT
CCGCTTACGAAAGTCGGCGGTACATTCCTCGCGATGAAAGCTGCTTCGGCCCCCGAAGAGTTGAAAGAGGGGGAAAAAGC
GATCGCCGTGCTCGGCGGGGAAGTAACCGCGACGGAAACATTCATGCTGCCATTTGAGGAAGGCGAACGGACGATCATTT
TCGTTCAAAAAACGAAAAAGACGCCGGCCCGATACCCGCGCAAACCGGGAACGCCAAATAAACAACCGATTCAGTAA

Upstream 100 bases:

>100_bases
GCGTCAATCCGGCCGATATTTCGATATTATTAGTGTATTTGGAACAAGGAAGAATCGCGCGCGTGTCGAATGAATAAATC
ATGAGGGAAAGGATTGGCTT

Downstream 100 bases:

>100_bases
CCCTTTGCGGACGCGGCGGGAAAGGGAAAGAGAAAAGAGGTTGTACCGGCTCCCGCCTCGCGTTCCGTTTCGAGGAAAAC
AAAGACGGCCATAAGCAGCG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MEATQFQAMLEERGISLSSQALAQFERYYELLVEWNEKMNLTAITDKPGVYVKHFFDSVSPAFYYDFSEPFSLCDVGAGA
GFPSIPLKICFPHLRVSIVDSLQKRIRFLQHLVGELGLKDIALYHDRAETFARQKGMRESFDVVTARAVARMPVLAELCL
PLTKVGGTFLAMKAASAPEELKEGEKAIAVLGGEVTATETFMLPFEEGERTIIFVQKTKKTPARYPRKPGTPNKQPIQ

Sequences:

>Translated_238_residues
MEATQFQAMLEERGISLSSQALAQFERYYELLVEWNEKMNLTAITDKPGVYVKHFFDSVSPAFYYDFSEPFSLCDVGAGA
GFPSIPLKICFPHLRVSIVDSLQKRIRFLQHLVGELGLKDIALYHDRAETFARQKGMRESFDVVTARAVARMPVLAELCL
PLTKVGGTFLAMKAASAPEELKEGEKAIAVLGGEVTATETFMLPFEEGERTIIFVQKTKKTPARYPRKPGTPNKQPIQ
>Mature_238_residues
MEATQFQAMLEERGISLSSQALAQFERYYELLVEWNEKMNLTAITDKPGVYVKHFFDSVSPAFYYDFSEPFSLCDVGAGA
GFPSIPLKICFPHLRVSIVDSLQKRIRFLQHLVGELGLKDIALYHDRAETFARQKGMRESFDVVTARAVARMPVLAELCL
PLTKVGGTFLAMKAASAPEELKEGEKAIAVLGGEVTATETFMLPFEEGERTIIFVQKTKKTPARYPRKPGTPNKQPIQ

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=216, Percent_Identity=31.9444444444444, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_GEOTN (A4ITW9)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001127518.1
- ProteinModelPortal:   A4ITW9
- SMR:   A4ITW9
- STRING:   A4ITW9
- GeneID:   4968359
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_3438
- NMPDR:   fig|420246.5.peg.3284
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   YELLVEW
- PhylomeDB:   A4ITW9
- ProtClustDB:   PRK00107
- BioCyc:   GTHE420246:GTNG_3438-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.- [C]

Molecular weight: Translated: 26656; Mature: 26656

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATQFQAMLEERGISLSSQALAQFERYYELLVEWNEKMNLTAITDKPGVYVKHFFDSVS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCC
PAFYYDFSEPFSLCDVGAGAGFPSIPLKICFPHLRVSIVDSLQKRIRFLQHLVGELGLKD
CEEEECCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IALYHDRAETFARQKGMRESFDVVTARAVARMPVLAELCLPLTKVGGTFLAMKAASAPEE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHH
LKEGEKAIAVLGGEVTATETFMLPFEEGERTIIFVQKTKKTPARYPRKPGTPNKQPIQ
HHCCCCEEEEECCCEEECEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEATQFQAMLEERGISLSSQALAQFERYYELLVEWNEKMNLTAITDKPGVYVKHFFDSVS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCC
PAFYYDFSEPFSLCDVGAGAGFPSIPLKICFPHLRVSIVDSLQKRIRFLQHLVGELGLKD
CEEEECCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IALYHDRAETFARQKGMRESFDVVTARAVARMPVLAELCLPLTKVGGTFLAMKAASAPEE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHH
LKEGEKAIAVLGGEVTATETFMLPFEEGERTIIFVQKTKKTPARYPRKPGTPNKQPIQ
HHCCCCEEEEECCCEEECEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA