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The map label for this gene is ald [H]

Identifier: 138897010

GI number: 138897010

Start: 3488370

End: 3489503

Strand: Direct

Name: ald [H]

Synonym: GTNG_3381

Alternate gene names: 138897010

Gene position: 3488370-3489503 (Clockwise)

Preceding gene: 138897009

Following gene: 138897011

Centisome position: 98.26

GC content: 54.32

Gene sequence:

>1134_bases
ATGATTATTGGAGTACCAAAGGAAATCAAAAATAACGAAAACCGTGTCGCGATTACGCCAGCAGGCGTTTTGTCATTCGT
TCAAGCAGGACATAAAGTGCTCATCGAAAAAGAGGCAGGAGTTGGAAGCGGTTTCAGCGACGACGATTACATCCGCGCTG
GAGCGCAAATCATTGAACGTGCCGAAGACGTTTGGGCACAGGCTGATATGGTAATGAAGGTAAAAGAGCCGCTGCCAAGT
GAATACGGCTATTTCCGTCCAGGTCTCATTTTGTTCACGTATTTGCATTTGGCGGCAGAGCCGGAATTGACTCGCGCCTT
AAAAGAAAGCGGTGTCATCGCCATCGCTTATGAGACAGTGCAAGTCGGCCGCACGCTGCCGTTGTTGACGCCGATGAGTG
AAGTCGCTGGACGGATGGCGGCGCAAATCGGGGCGCAATTCTTAGAAAAACCGTACGGAGGAAAAGGCATTTTGCTTAGC
GGCGTTCCTGGCGTTGCCCGCGGTAAAGTAACGATCATCGGCGGTGGGGTCGTCGGTACGAACGCAGCAAAAGTCGCGGT
CGGTCTTGGTGCAGACGTGACGATCATCGACTTGAACGCTGACCGTCTCCGTGAGCTCGACGACATTTTCGGCCACCAAA
TTACAACGCTCATGTCCAACCCGATGAACATCGCTGAAGCAGTTGCTGAAGCTGACCTTGTCATCGGTGCGGTTCTCATC
CCAGGCGCACGGGCACCGAAACTCGTCACGGAAGATATGGTAAAAGCAATGAAACCGGGTTCGGTCATTGTCGATGTCGC
CATCGACCAAGGCGGTATCGTTGAAACGAGCGACCACGTCACGACACATGACAACCCGACCTACGTCAAACACGGTGTCG
TCCACTATGCAGTCGCTAACATGCCGGGTGCCGTCCCGCGCACTTCGACGATCGCCTTGACGAACGTCACGATCCCATAC
GCTTTACAAATCGCTAATAAAGGCGTTATCCAAGCCATTGCTGACAACCCGGCGCTCGAGCTTGGCCTCAACGTCGCCAA
CGGCGAAATCACGTACGAAGCGGTTGCCCGCGACCTCGGTTACCGCTACGTACCGGCCCGCGAAGCGCTCGGCAAAACGT
TGGCGGCCAACTAA

Upstream 100 bases:

>100_bases
AAACTGTGTGCTTCTTTTCTCTTTTTTGAACAATGTCCGACCTGCTCCTTTTCTTTTATACTATAGGTGCAGCCACTTCT
TACGAGGAGGAACGATATTG

Downstream 100 bases:

>100_bases
ACGCTAACGAATGCGGCCGGCAAAGCGCGTTGTCGGCCGCATGTTCATCGCTGCCCATTCACTGCCATAAATTGCCGTCC
CATACGGACGGCTTTTTTTA

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIIGVPKEIKNNENRVAITPAGVLSFVQAGHKVLIEKEAGVGSGFSDDDYIRAGAQIIERAEDVWAQADMVMKVKEPLPS
EYGYFRPGLILFTYLHLAAEPELTRALKESGVIAIAYETVQVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPYGGKGILLS
GVPGVARGKVTIIGGGVVGTNAAKVAVGLGADVTIIDLNADRLRELDDIFGHQITTLMSNPMNIAEAVAEADLVIGAVLI
PGARAPKLVTEDMVKAMKPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVANMPGAVPRTSTIALTNVTIPY
ALQIANKGVIQAIADNPALELGLNVANGEITYEAVARDLGYRYVPAREALGKTLAAN

Sequences:

>Translated_377_residues
MIIGVPKEIKNNENRVAITPAGVLSFVQAGHKVLIEKEAGVGSGFSDDDYIRAGAQIIERAEDVWAQADMVMKVKEPLPS
EYGYFRPGLILFTYLHLAAEPELTRALKESGVIAIAYETVQVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPYGGKGILLS
GVPGVARGKVTIIGGGVVGTNAAKVAVGLGADVTIIDLNADRLRELDDIFGHQITTLMSNPMNIAEAVAEADLVIGAVLI
PGARAPKLVTEDMVKAMKPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVANMPGAVPRTSTIALTNVTIPY
ALQIANKGVIQAIADNPALELGLNVANGEITYEAVARDLGYRYVPAREALGKTLAAN
>Mature_377_residues
MIIGVPKEIKNNENRVAITPAGVLSFVQAGHKVLIEKEAGVGSGFSDDDYIRAGAQIIERAEDVWAQADMVMKVKEPLPS
EYGYFRPGLILFTYLHLAAEPELTRALKESGVIAIAYETVQVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPYGGKGILLS
GVPGVARGKVTIIGGGVVGTNAAKVAVGLGADVTIIDLNADRLRELDDIFGHQITTLMSNPMNIAEAVAEADLVIGAVLI
PGARAPKLVTEDMVKAMKPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVANMPGAVPRTSTIALTNVTIPY
ALQIANKGVIQAIADNPALELGLNVANGEITYEAVARDLGYRYVPAREALGKTLAAN

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=347, Percent_Identity=30.835734870317, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI122939153, Length=347, Percent_Identity=30.835734870317, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1787887, Length=383, Percent_Identity=32.3759791122715, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17550456, Length=374, Percent_Identity=29.4117647058824, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39729; Mature: 39729

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNNENRVAITPAGVLSFVQAGHKVLIEKEAGVGSGFSDDDYIRAGAQIIER
CEECCCHHHCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AEDVWAQADMVMKVKEPLPSEYGYFRPGLILFTYLHLAAEPELTRALKESGVIAIAYETV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEEEEEE
QVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPYGGKGILLSGVPGVARGKVTIIGGGVVGT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKVAVGLGADVTIIDLNADRLRELDDIFGHQITTLMSNPMNIAEAVAEADLVIGAVLI
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEC
PGARAPKLVTEDMVKAMKPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVAN
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCEECCCCCCHHHHCHHEEEECC
MPGAVPRTSTIALTNVTIPYALQIANKGVIQAIADNPALELGLNVANGEITYEAVARDLG
CCCCCCCCCEEEEEEECCCEEEEECCCCEEEEECCCCCEEEEEEECCCEEEHHHHHHHCC
YRYVPAREALGKTLAAN
CEECCHHHHHHHHHCCC
>Mature Secondary Structure
MIIGVPKEIKNNENRVAITPAGVLSFVQAGHKVLIEKEAGVGSGFSDDDYIRAGAQIIER
CEECCCHHHCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AEDVWAQADMVMKVKEPLPSEYGYFRPGLILFTYLHLAAEPELTRALKESGVIAIAYETV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEEEEEE
QVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPYGGKGILLSGVPGVARGKVTIIGGGVVGT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKVAVGLGADVTIIDLNADRLRELDDIFGHQITTLMSNPMNIAEAVAEADLVIGAVLI
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEC
PGARAPKLVTEDMVKAMKPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVAN
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCEECCCCCCHHHHCHHEEEECC
MPGAVPRTSTIALTNVTIPYALQIANKGVIQAIADNPALELGLNVANGEITYEAVARDLG
CCCCCCCCCEEEEEEECCCEEEEECCCCEEEEECCCCCEEEEEEECCCEEEHHHHHHHCC
YRYVPAREALGKTLAAN
CEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]