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The map label for this gene is pbuO [H]

Identifier: 138896984

GI number: 138896984

Start: 3463290

End: 3464591

Strand: Direct

Name: pbuO [H]

Synonym: GTNG_3355

Alternate gene names: 138896984

Gene position: 3463290-3464591 (Clockwise)

Preceding gene: 138896983

Following gene: 138896988

Centisome position: 97.55

GC content: 54.99

Gene sequence:

>1302_bases
ATGATTCGTTTTCCGAACAACACTACCGATCGCAAAACGGAATTTTTCGCTGGTTTGACGACGTTTTTAACGATGGTATA
CATCATTGTGGTCAATCCGGTTATCTTGTCGGACGCCGGTGTCCCGTTTGAACAGGCGTTTACTGCGACCGTCATCGCCA
CCGTGGTTGGCACGCTTTGGATGGCCATTTTTGCCAACTATCCGATCGCTATCGCTCCCGGCATGGGACTCAACGCCTAT
TTCGCCTATTCGGTCGTCGGTTCGCACGGCAACATTTCGTACGAAACCGCGTTTTCCGCTGTGTTTGTCGCGGGGTTACT
GTTTTTGCTGCTGTCACTCACCCCATTGCGCAGCAAGCTAATCGAAGTGATCCCTGACAGCTTAAAACATGGCATCACCG
CCGGCATTGGCCTGTTTATCGCCTTTATCGGCTTGCGGCTAACTGGGATCATCCAATCCCACCCACAAAACTTAGTAGCG
CTCGGCGACTTGCATGCTCCATCAGCTATGTTGACGCTGATTGGCCTCGCCGTAACGCTCGTTCTTATGATACGTCGCGT
CAACGGCGCGCTCTTTTACGGCATGATTATCACCGGGGTGATTGCCTACGCGACCGGTCAATTGAAATTTGACGAAGGAA
TCATGTCGTTGCCGTCGTTGCCGGATGGGGTTGTCGTTGCCAATCCGTTTGCGGCCGTTGGTGACGTCATCAGCCATCAT
TTGTATGCCGTCGTCTTTTCCTTTTTGCTTGTCACGCTGTTTGACACGACTGGCACAATGATCGGCGTCGCCCAACAAGC
AGGACTGATGAAAGGGAACACGCTGCCGCGCGCCCGCGAAGCGCTCTTAGCTGACTCAGTCGCGACTACGGTCGGAGCCA
TGTTTGGGACAAGCCCAACATCAGCGTACATCGAGTCGTCCACCGGTGTCGCTGCCGGCGGACGGACCGGATGGACAGCG
CTCACTGTCGCCGTACTGTTTATCATCGCCGCCTTTTTCAGCCCGCTAGTGGGCGCGGTCGCTGGGTTATCCGCCATCAC
TGCTCCGGCGCTCATTATCGTCGGCAGCTTGATGATCAGCAACATCGCCCATATTTCATGGCATGAACTCGACGAGGCGT
TCCCGGCATTTCTCATCATCTTAAGCATGCCGCTCACTTCCAGCATCGCGACCGGCATCGCGCTCGGCTTCATTTCGTAC
CCGCTCTTAAAAATTGCCCGCGGCCGCTGGCGCGACGTGCCTCCGCTGTTGTATGTGCTTGCCGTGCTGTTCTTTTTGCA
GCTGGCGTTTTTGCCACACTGA

Upstream 100 bases:

>100_bases
GGAGAGACGGGGGCGCTTAGGGCGCTCCCGTTGCTTTTTGAAGAAAAAACGAATATTATTATATTTGTTTGTAATATTGT
TCGTTTTTAGGAGGTTGCCA

Downstream 100 bases:

>100_bases
TGGGAGGTCACACGACCATTGGCCACATCGGTTTTCAACCGTAAAAAAGCCCGCTTATACGCGGGATTTTTTATTGCATT
GGCTGCGGAGCATCTCAATG

Product: guanine-hypoxanthine permease-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIRFPNNTTDRKTEFFAGLTTFLTMVYIIVVNPVILSDAGVPFEQAFTATVIATVVGTLWMAIFANYPIAIAPGMGLNAY
FAYSVVGSHGNISYETAFSAVFVAGLLFLLLSLTPLRSKLIEVIPDSLKHGITAGIGLFIAFIGLRLTGIIQSHPQNLVA
LGDLHAPSAMLTLIGLAVTLVLMIRRVNGALFYGMIITGVIAYATGQLKFDEGIMSLPSLPDGVVVANPFAAVGDVISHH
LYAVVFSFLLVTLFDTTGTMIGVAQQAGLMKGNTLPRAREALLADSVATTVGAMFGTSPTSAYIESSTGVAAGGRTGWTA
LTVAVLFIIAAFFSPLVGAVAGLSAITAPALIIVGSLMISNIAHISWHELDEAFPAFLIILSMPLTSSIATGIALGFISY
PLLKIARGRWRDVPPLLYVLAVLFFLQLAFLPH

Sequences:

>Translated_433_residues
MIRFPNNTTDRKTEFFAGLTTFLTMVYIIVVNPVILSDAGVPFEQAFTATVIATVVGTLWMAIFANYPIAIAPGMGLNAY
FAYSVVGSHGNISYETAFSAVFVAGLLFLLLSLTPLRSKLIEVIPDSLKHGITAGIGLFIAFIGLRLTGIIQSHPQNLVA
LGDLHAPSAMLTLIGLAVTLVLMIRRVNGALFYGMIITGVIAYATGQLKFDEGIMSLPSLPDGVVVANPFAAVGDVISHH
LYAVVFSFLLVTLFDTTGTMIGVAQQAGLMKGNTLPRAREALLADSVATTVGAMFGTSPTSAYIESSTGVAAGGRTGWTA
LTVAVLFIIAAFFSPLVGAVAGLSAITAPALIIVGSLMISNIAHISWHELDEAFPAFLIILSMPLTSSIATGIALGFISY
PLLKIARGRWRDVPPLLYVLAVLFFLQLAFLPH
>Mature_433_residues
MIRFPNNTTDRKTEFFAGLTTFLTMVYIIVVNPVILSDAGVPFEQAFTATVIATVVGTLWMAIFANYPIAIAPGMGLNAY
FAYSVVGSHGNISYETAFSAVFVAGLLFLLLSLTPLRSKLIEVIPDSLKHGITAGIGLFIAFIGLRLTGIIQSHPQNLVA
LGDLHAPSAMLTLIGLAVTLVLMIRRVNGALFYGMIITGVIAYATGQLKFDEGIMSLPSLPDGVVVANPFAAVGDVISHH
LYAVVFSFLLVTLFDTTGTMIGVAQQAGLMKGNTLPRAREALLADSVATTVGAMFGTSPTSAYIESSTGVAAGGRTGWTA
LTVAVLFIIAAFFSPLVGAVAGLSAITAPALIIVGSLMISNIAHISWHELDEAFPAFLIILSMPLTSSIATGIALGFISY
PLLKIARGRWRDVPPLLYVLAVLFFLQLAFLPH

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=429, Percent_Identity=43.3566433566434, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1790150, Length=430, Percent_Identity=42.093023255814, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1790499, Length=425, Percent_Identity=35.5294117647059, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI48994909, Length=412, Percent_Identity=35.4368932038835, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45752; Mature: 45752

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFPNNTTDRKTEFFAGLTTFLTMVYIIVVNPVILSDAGVPFEQAFTATVIATVVGTLW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
MAIFANYPIAIAPGMGLNAYFAYSVVGSHGNISYETAFSAVFVAGLLFLLLSLTPLRSKL
HHHHHCCCEEEECCCCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEVIPDSLKHGITAGIGLFIAFIGLRLTGIIQSHPQNLVALGDLHAPSAMLTLIGLAVTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
VLMIRRVNGALFYGMIITGVIAYATGQLKFDEGIMSLPSLPDGVVVANPFAAVGDVISHH
HHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCCEEECCHHHHHHHHHHH
LYAVVFSFLLVTLFDTTGTMIGVAQQAGLMKGNTLPRAREALLADSVATTVGAMFGTSPT
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SAYIESSTGVAAGGRTGWTALTVAVLFIIAAFFSPLVGAVAGLSAITAPALIIVGSLMIS
HHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAHISWHELDEAFPAFLIILSMPLTSSIATGIALGFISYPLLKIARGRWRDVPPLLYVL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AVLFFLQLAFLPH
HHHHHHHHHHCCC
>Mature Secondary Structure
MIRFPNNTTDRKTEFFAGLTTFLTMVYIIVVNPVILSDAGVPFEQAFTATVIATVVGTLW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
MAIFANYPIAIAPGMGLNAYFAYSVVGSHGNISYETAFSAVFVAGLLFLLLSLTPLRSKL
HHHHHCCCEEEECCCCCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEVIPDSLKHGITAGIGLFIAFIGLRLTGIIQSHPQNLVALGDLHAPSAMLTLIGLAVTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
VLMIRRVNGALFYGMIITGVIAYATGQLKFDEGIMSLPSLPDGVVVANPFAAVGDVISHH
HHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCCEEECCHHHHHHHHHHH
LYAVVFSFLLVTLFDTTGTMIGVAQQAGLMKGNTLPRAREALLADSVATTVGAMFGTSPT
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SAYIESSTGVAAGGRTGWTALTVAVLFIIAAFFSPLVGAVAGLSAITAPALIIVGSLMIS
HHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAHISWHELDEAFPAFLIILSMPLTSSIATGIALGFISYPLLKIARGRWRDVPPLLYVL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AVLFFLQLAFLPH
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]