Click here to switch to the map view.

The map label for this gene is yclM [H]

Identifier: 138896751

GI number: 138896751

Start: 3213775

End: 3215151

Strand: Reverse

Name: yclM [H]

Synonym: GTNG_3114

Alternate gene names: 138896751

Gene position: 3215151-3213775 (Counterclockwise)

Preceding gene: 138896752

Following gene: 138896750

Centisome position: 90.56

GC content: 52.07

Gene sequence:

>1377_bases
ATGAAAGTAGCCAAATTTGGTGGAAGTTCGGTGGCGAGCGCCGCGCAATTCCGCAAGGTGGCTGATATTGTCAGTTCGGA
CATTGAACGCCGCATCATCGTCGTATCAGCACCCGGTAAGCGATGCAAAGACGACGTGAAAGTGACGGATATGCTTATTC
GGCTGGCTGAACAAGTGTTGGCTGGTGAACCGTACGACGAAACGATGCAGGCGATCACGAAGCGGTACGCTGACATTGCC
GATGAGCTCGAACTGGAAGCCAACGGTTTTTTAGCTGAGCTCACAGATGATTTACAGTCAAAAATTAGCGCCTATCGAAA
TGAGCCAGCCCGCCTGCTTGATGCGATAAAAGCGAGCGGTGAAGATCATAATGCCCGGTTGATGGCGCTCTATTTGCAAG
ATTTTGGGCTCGAGGCGAGCTATGTTAGCCCGCTTGAGGCCGGCATTATCGTGACGGACGAACCTGGCAACGCCCAAGTG
CTTCCGGAGTCGTACGACAAGCTGCGGCAGCTGCGTGAGCGTCGCGGTGTGCTCGTGATCCCGGGCTTTTTCGGCTATTC
CCTATCCGGCCATATCGTCACCTTCCCGCGCGGTGGATCGGATATTACCGGTTCGATCGTTGCCGCAGGTGTAAAAGCCG
ACGTGTATGAAAACTTTACTGACGTTGATTCGATTTATTGCGTCAATCCTTCGATTGTCGCCGACGCGCGCAAACTGAAA
GAAATTACGTATCGGGAAATGCGCGAGCTGTCGTATTCCGGCTTTTCCGTTTTTCATGATGAGGCGCTCGAGCCGGTATA
TCGGGCTGGCATCCCGGTTTGCGTGAAAAACACGAATAACCCGGCTGCGCCGGGTACGTGGATCGTTGCCAAACGCAACC
ATGTAGATGAACCGGTTGCGGGTATTGCCAGCGATACCGGCTTTTGCAGCATCAACATTAGTAAATACTTAATGAACCGC
GAAATCGGCTTTGGTCGGCGCGTGTTGCAAATTTTAGAAGACGAGGGCATTTCATATGAACATACGCCGTCTGGTATCGA
TAACATGTCAGTTATTTTGCGGGCAAGTCAGCTTGCCGATGGAAAAGATGAGCGCATTTTAGCCCGCATCCGCGATGAGC
TCGTCGTCGATGAAGTGACGATTGAGTACGGATTGGCGCTTATCATGGTGGTTGGCGAAGGGATGGAAAAAATGGTTGGG
ATGGCAGCCAAAGCGGCGACGGCGCTCGCGAATGCCAACATTAACTTAGAGATGATCAACCAAGGATCATCGGAGGTCAG
CATGATGTTTGGCGTCAAAGAGGATGTCGTCAACGAGGCGGTTCGTGCGCTATATCACGCCTACTTTCAAGAGTTGACCG
TTAGTCAGCCGTCATAA

Upstream 100 bases:

>100_bases
ACCATTCGCGGAAATTTCTAAAAAATGAATGATTTCACGAGCGGAAAAATGTGATATGATAAACAATTATAAAGACTTTC
GACAGAAAGGGTCATGGAAC

Downstream 100 bases:

>100_bases
GGAAAAGGATGTCCGCGTCGCGGACATCCTTTTTTCATTGATTGTATCGTTTGTTTCCGCCATTGCTGCTTTTCGTACAT
AGAAAGGAAGGGCTCGGAAA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKVAKFGGSSVASAAQFRKVADIVSSDIERRIIVVSAPGKRCKDDVKVTDMLIRLAEQVLAGEPYDETMQAITKRYADIA
DELELEANGFLAELTDDLQSKISAYRNEPARLLDAIKASGEDHNARLMALYLQDFGLEASYVSPLEAGIIVTDEPGNAQV
LPESYDKLRQLRERRGVLVIPGFFGYSLSGHIVTFPRGGSDITGSIVAAGVKADVYENFTDVDSIYCVNPSIVADARKLK
EITYREMRELSYSGFSVFHDEALEPVYRAGIPVCVKNTNNPAAPGTWIVAKRNHVDEPVAGIASDTGFCSINISKYLMNR
EIGFGRRVLQILEDEGISYEHTPSGIDNMSVILRASQLADGKDERILARIRDELVVDEVTIEYGLALIMVVGEGMEKMVG
MAAKAATALANANINLEMINQGSSEVSMMFGVKEDVVNEAVRALYHAYFQELTVSQPS

Sequences:

>Translated_458_residues
MKVAKFGGSSVASAAQFRKVADIVSSDIERRIIVVSAPGKRCKDDVKVTDMLIRLAEQVLAGEPYDETMQAITKRYADIA
DELELEANGFLAELTDDLQSKISAYRNEPARLLDAIKASGEDHNARLMALYLQDFGLEASYVSPLEAGIIVTDEPGNAQV
LPESYDKLRQLRERRGVLVIPGFFGYSLSGHIVTFPRGGSDITGSIVAAGVKADVYENFTDVDSIYCVNPSIVADARKLK
EITYREMRELSYSGFSVFHDEALEPVYRAGIPVCVKNTNNPAAPGTWIVAKRNHVDEPVAGIASDTGFCSINISKYLMNR
EIGFGRRVLQILEDEGISYEHTPSGIDNMSVILRASQLADGKDERILARIRDELVVDEVTIEYGLALIMVVGEGMEKMVG
MAAKAATALANANINLEMINQGSSEVSMMFGVKEDVVNEAVRALYHAYFQELTVSQPS
>Mature_458_residues
MKVAKFGGSSVASAAQFRKVADIVSSDIERRIIVVSAPGKRCKDDVKVTDMLIRLAEQVLAGEPYDETMQAITKRYADIA
DELELEANGFLAELTDDLQSKISAYRNEPARLLDAIKASGEDHNARLMALYLQDFGLEASYVSPLEAGIIVTDEPGNAQV
LPESYDKLRQLRERRGVLVIPGFFGYSLSGHIVTFPRGGSDITGSIVAAGVKADVYENFTDVDSIYCVNPSIVADARKLK
EITYREMRELSYSGFSVFHDEALEPVYRAGIPVCVKNTNNPAAPGTWIVAKRNHVDEPVAGIASDTGFCSINISKYLMNR
EIGFGRRVLQILEDEGISYEHTPSGIDNMSVILRASQLADGKDERILARIRDELVVDEVTIEYGLALIMVVGEGMEKMVG
MAAKAATALANANINLEMINQGSSEVSMMFGVKEDVVNEAVRALYHAYFQELTVSQPS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=471, Percent_Identity=30.7855626326964, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1790455, Length=466, Percent_Identity=27.0386266094421, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790376, Length=293, Percent_Identity=26.6211604095563, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=499, Percent_Identity=25.8517034068136, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50138; Mature: 50138

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKFGGSSVASAAQFRKVADIVSSDIERRIIVVSAPGKRCKDDVKVTDMLIRLAEQVL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGEPYDETMQAITKRYADIADELELEANGFLAELTDDLQSKISAYRNEPARLLDAIKASG
CCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
EDHNARLMALYLQDFGLEASYVSPLEAGIIVTDEPGNAQVLPESYDKLRQLRERRGVLVI
CCCCCEEEEEEEHHHCCCCCCCCCCCCCEEEECCCCCCEECCHHHHHHHHHHHHCCEEEE
PGFFGYSLSGHIVTFPRGGSDITGSIVAAGVKADVYENFTDVDSIYCVNPSIVADARKLK
CCHHCCCCCCCEEEECCCCCCCCCHHHEECCHHHHHHCCCCCCCEEEECCHHHHHHHHHH
EITYREMRELSYSGFSVFHDEALEPVYRAGIPVCVKNTNNPAAPGTWIVAKRNHVDEPVA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCHH
GIASDTGFCSINISKYLMNREIGFGRRVLQILEDEGISYEHTPSGIDNMSVILRASQLAD
HCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEHHHHCC
GKDERILARIRDELVVDEVTIEYGLALIMVVGEGMEKMVGMAAKAATALANANINLEMIN
CCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEC
QGSSEVSMMFGVKEDVVNEAVRALYHAYFQELTVSQPS
CCCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVAKFGGSSVASAAQFRKVADIVSSDIERRIIVVSAPGKRCKDDVKVTDMLIRLAEQVL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGEPYDETMQAITKRYADIADELELEANGFLAELTDDLQSKISAYRNEPARLLDAIKASG
CCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
EDHNARLMALYLQDFGLEASYVSPLEAGIIVTDEPGNAQVLPESYDKLRQLRERRGVLVI
CCCCCEEEEEEEHHHCCCCCCCCCCCCCEEEECCCCCCEECCHHHHHHHHHHHHCCEEEE
PGFFGYSLSGHIVTFPRGGSDITGSIVAAGVKADVYENFTDVDSIYCVNPSIVADARKLK
CCHHCCCCCCCEEEECCCCCCCCCHHHEECCHHHHHHCCCCCCCEEEECCHHHHHHHHHH
EITYREMRELSYSGFSVFHDEALEPVYRAGIPVCVKNTNNPAAPGTWIVAKRNHVDEPVA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCHH
GIASDTGFCSINISKYLMNREIGFGRRVLQILEDEGISYEHTPSGIDNMSVILRASQLAD
HCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEHHHHCC
GKDERILARIRDELVVDEVTIEYGLALIMVVGEGMEKMVGMAAKAATALANANINLEMIN
CCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEC
QGSSEVSMMFGVKEDVVNEAVRALYHAYFQELTVSQPS
CCCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]