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The map label for this gene is bsdA [H]

Identifier: 138896630

GI number: 138896630

Start: 3089532

End: 3090449

Strand: Direct

Name: bsdA [H]

Synonym: GTNG_2993

Alternate gene names: 138896630

Gene position: 3089532-3090449 (Clockwise)

Preceding gene: 138896609

Following gene: 138896633

Centisome position: 87.02

GC content: 51.53

Gene sequence:

>918_bases
ATGATCGATGTGAAACAGCTAAAATACTTCGTTACCATCGCCGAAGAGGGACAAATCACGAAGGCGGCACAAAAACTTCA
TATCGCTCAGCCGCCGCTCAGCCAACAGTTGAAGCAACTCGAAGAAAAGCTCGGCGTCCCATTGTTTGACCGGAAAGCGA
AAAAAATGGAGCTCACCGCACCGGGCAAATTGTTTTACGAAAAGGCGAAACAACTGTTGCACCAGCTAGATGACGCCATC
GCGGAAGTGAAAGAAATCGAAACGGGGGCAACGGGAACATTGGCTGTCGGCTGCGTCAAATCGTGCTTTTCCTATTTGGC
CCAAACGATCCGCCAATTCCACGAACAGTTTCCGAACGTCACGTTTCATCTTCGCGAAGGAGATACGTTTTCCATTTGCC
AGCTTCTTCAAGAGCGGCACATTGACATCGGCATTGTCCGCCTACCGATCGATTCGTCTCATTACGACATCATTCCGCTG
CCAAGCGACCGTTATGTCGCCGTCTTGCCTGAGCATTGGGCATTGAGCCGCTCTTCCTTATCGATGCGCGATTTTGCAGA
GTGGCCGCTCCTCCTTCTTCACCGTGTACACGGTGCCGGACAGTACGAACGGGTCGTCGAGGAATGCCGCCGTCATGGGT
TTGAGCCAAACGTTCTATGCGAATGCCCGGATGCAACCATTTTGCTTTCCCTTGTCGCCCAAGGCATGGGCGCCACCATT
GTGCCACAGTCAACCGTTTCCTTGTTTCGTCTTCCTGGCATTCGCGTCCTCCCAATTGTTGATTCATCAATGAAAGCAGA
AGCCGCCGTCATCATCGATCGACAACGCTATGTGCCCAAAAGCGTCCATCATTTTCTCAACCTGTTGCAACAAACCGTCG
CCATGCCCATCATGAACGAAAAAAGCAACCATGTGTGA

Upstream 100 bases:

>100_bases
AAATTAATATAACATATGATTTCATATATGAAAAATACAAAAAGAATCAACTATTCTATATGAAAAATATATAGAAGCGG
TAGAGAAAGGAGACACGGAC

Downstream 100 bases:

>100_bases
AACGAAATGAGGCAGAAAGGAGCAATCGAGTTCCGATGCCAAGTGGGAGAAAGCCAACTGCCGAGGGAATGCGAATGGTC
ATACCGTGGGATCGATGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MIDVKQLKYFVTIAEEGQITKAAQKLHIAQPPLSQQLKQLEEKLGVPLFDRKAKKMELTAPGKLFYEKAKQLLHQLDDAI
AEVKEIETGATGTLAVGCVKSCFSYLAQTIRQFHEQFPNVTFHLREGDTFSICQLLQERHIDIGIVRLPIDSSHYDIIPL
PSDRYVAVLPEHWALSRSSLSMRDFAEWPLLLLHRVHGAGQYERVVEECRRHGFEPNVLCECPDATILLSLVAQGMGATI
VPQSTVSLFRLPGIRVLPIVDSSMKAEAAVIIDRQRYVPKSVHHFLNLLQQTVAMPIMNEKSNHV

Sequences:

>Translated_305_residues
MIDVKQLKYFVTIAEEGQITKAAQKLHIAQPPLSQQLKQLEEKLGVPLFDRKAKKMELTAPGKLFYEKAKQLLHQLDDAI
AEVKEIETGATGTLAVGCVKSCFSYLAQTIRQFHEQFPNVTFHLREGDTFSICQLLQERHIDIGIVRLPIDSSHYDIIPL
PSDRYVAVLPEHWALSRSSLSMRDFAEWPLLLLHRVHGAGQYERVVEECRRHGFEPNVLCECPDATILLSLVAQGMGATI
VPQSTVSLFRLPGIRVLPIVDSSMKAEAAVIIDRQRYVPKSVHHFLNLLQQTVAMPIMNEKSNHV
>Mature_305_residues
MIDVKQLKYFVTIAEEGQITKAAQKLHIAQPPLSQQLKQLEEKLGVPLFDRKAKKMELTAPGKLFYEKAKQLLHQLDDAI
AEVKEIETGATGTLAVGCVKSCFSYLAQTIRQFHEQFPNVTFHLREGDTFSICQLLQERHIDIGIVRLPIDSSHYDIIPL
PSDRYVAVLPEHWALSRSSLSMRDFAEWPLLLLHRVHGAGQYERVVEECRRHGFEPNVLCECPDATILLSLVAQGMGATI
VPQSTVSLFRLPGIRVLPIVDSSMKAEAAVIIDRQRYVPKSVHHFLNLLQQTVAMPIMNEKSNHV

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=272, Percent_Identity=30.5147058823529, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI157672245, Length=299, Percent_Identity=25.4180602006689, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI2367136, Length=303, Percent_Identity=27.7227722772277, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI145693105, Length=293, Percent_Identity=23.5494880546075, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787806, Length=262, Percent_Identity=27.0992366412214, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=26.7489711934156, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790262, Length=284, Percent_Identity=26.7605633802817, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=22.7091633466135, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=25.5060728744939, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787601, Length=238, Percent_Identity=27.3109243697479, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=26.9076305220884, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081904, Length=196, Percent_Identity=29.0816326530612, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=26.7441860465116, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788481, Length=205, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=170, Percent_Identity=28.8235294117647, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=20.8163265306122, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789639, Length=128, Percent_Identity=26.5625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=144, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786713, Length=173, Percent_Identity=24.8554913294798, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=291, Percent_Identity=19.5876288659794, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790783, Length=113, Percent_Identity=29.2035398230088, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790208, Length=149, Percent_Identity=28.1879194630872, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34434; Mature: 34434

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVKQLKYFVTIAEEGQITKAAQKLHIAQPPLSQQLKQLEEKLGVPLFDRKAKKMELTA
CCCHHHEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCC
PGKLFYEKAKQLLHQLDDAIAEVKEIETGATGTLAVGCVKSCFSYLAQTIRQFHEQFPNV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TFHLREGDTFSICQLLQERHIDIGIVRLPIDSSHYDIIPLPSDRYVAVLPEHWALSRSSL
EEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCEEEECCCHHHCCCCCC
SMRDFAEWPLLLLHRVHGAGQYERVVEECRRHGFEPNVLCECPDATILLSLVAQGMGATI
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEE
VPQSTVSLFRLPGIRVLPIVDSSMKAEAAVIIDRQRYVPKSVHHFLNLLQQTVAMPIMNE
CCHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECHHCCHHHHHHHHHHHHHHHHCCCCCC
KSNHV
CCCCC
>Mature Secondary Structure
MIDVKQLKYFVTIAEEGQITKAAQKLHIAQPPLSQQLKQLEEKLGVPLFDRKAKKMELTA
CCCHHHEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCC
PGKLFYEKAKQLLHQLDDAIAEVKEIETGATGTLAVGCVKSCFSYLAQTIRQFHEQFPNV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TFHLREGDTFSICQLLQERHIDIGIVRLPIDSSHYDIIPLPSDRYVAVLPEHWALSRSSL
EEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCEEEECCCHHHCCCCCC
SMRDFAEWPLLLLHRVHGAGQYERVVEECRRHGFEPNVLCECPDATILLSLVAQGMGATI
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEE
VPQSTVSLFRLPGIRVLPIVDSSMKAEAAVIIDRQRYVPKSVHHFLNLLQQTVAMPIMNE
CCHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECHHCCHHHHHHHHHHHHHHHHCCCCCC
KSNHV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]