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The map label for this gene is menF [H]

Identifier: 138896411

GI number: 138896411

Start: 2888011

End: 2889396

Strand: Reverse

Name: menF [H]

Synonym: GTNG_2774

Alternate gene names: 138896411

Gene position: 2889396-2888011 (Counterclockwise)

Preceding gene: 138896415

Following gene: 138896410

Centisome position: 81.38

GC content: 57.14

Gene sequence:

>1386_bases
GTGAGGGTCGTGGCAATTGTATCTCAACATAAAATCCAACAACAACTGCACGAGTTGGCGGCAAATGCCGCACGGCCGTT
TGTGAGCTGGACGGAACAGTTGGAAGACATCGATCCGGTTTCTTTTTTTATGCAAGGGCCATCCTGCGGGTTTCGCGAGC
GATTTTTTTGGTCTGACCGAAGTGGCAGCACCATCTTTGTCGGCCTTGGCTCGGCTTATACGATCGAAACAGAGGAAACA
GACGGACGGTTTCATTATGTCGAACAGCAGCGCCAACGATGGGTTGAACAAATCGAGCGGCACGGTGAAGCGGATATGCC
GCCGCTATTGTTTGGCGGGTTTGCTTTTGACCCGCATCAACCGCGCACATCCGTATGGCGCGGTTTTCCGGCCGGAAAAC
TCATCGCGCCGACGGTGCTTCTCTCGGTTAAAAACGGACGGACGCTGCTGACGGTCACGCTGCCAACTGACGGCGTAGAG
CGGGATATGGAGCGAATCGGGCGGCTGTTTAGACGGATCGAGGAGCTGCAGCCGGTTTCGGCAACGCTTCCAGCGCTCGT
CTCAGATGAAGAGGAGGAAAAAGAGCGGTGGCTCGCGGCGGTCCGTCAAGCCATTGCTTCCATTCGTGCCGGAAGGTTGG
ATAAAGTCGTGCTCGCTCGTTCGCGGCGGTTGGCGTTCACCGGGACGATTGACGCTGCCGCTGTGCTGGCGCGGCTTCGT
GAACAGCAGCCGTTTGCTTACCTTTTTGCGTTTGAACAAGATGGCCGCTGTTTTATTGGCGCCTCGCCTGAGCAGCTCGT
TCAAAAAGAAGGAGAGATGTGCCAATCGGTGTGCTTGGCGGGATCCGTGCGCCGCGGAAGCAGCCCTGGAGAAGATGAAC
AGCTTGGCGCCTGGCTGCAGGCTGATTCGAAAAACCGTGAGGAACATCAATTCGTTGTGCACATGATCCGTCGCGTGTTT
TCTTCCGTTTGCGAGACGGTGGAGATGCCGACAGCACCGCAGCTTCTGAAGCTGCCGCATATCCAACATTTGTGCACGCC
AGTTGTCGGCCGCGGCTGCCAGGAGCCATCCGTGTTGCGTCTTGTTGAAATGATGCACCCAACGCCGGCGCTCGGCGGAA
CGCCACGCGAACGAGCGATGGAAGCGATCCGTTCTCTTGAACCGCTTGACCGCGGGTGGTACGCCGCTCCGATCGGCTGG
GTGGATGCTGACGGCAACGGGGAATGGGCAGTTGCCATTCGCTCCGGCTTGCTTGACGTCGATAAAGCCATGCTGTTTGC
CGGCTGCGGCATCGTCGCCCATTCAGATCCAACGAGCGAGTACGAAGAGACGAACGTCAAAATGGCTCCGATGTTATCGT
CCTTAGGAGTGATGAGCAATGACTAA

Upstream 100 bases:

>100_bases
GAAAACGTTTCAGGTGTGTCAACCCTTTCCGATAGATAAATGATTTCCTATTATATATAATGAAAGTAGGGGATGGAGAA
GGAATCTAACGAAAGAAAGC

Downstream 100 bases:

>100_bases
CGCGTTGTCATGGTATGTGGCCGCTTTTGTTGACGGGCTTGTGCAAGCCGGGGTGACAGAAGCCGTCATCAGCCCTGGTT
CGCGCTCGACGCCGCTCGCG

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRVVAIVSQHKIQQQLHELAANAARPFVSWTEQLEDIDPVSFFMQGPSCGFRERFFWSDRSGSTIFVGLGSAYTIETEET
DGRFHYVEQQRQRWVEQIERHGEADMPPLLFGGFAFDPHQPRTSVWRGFPAGKLIAPTVLLSVKNGRTLLTVTLPTDGVE
RDMERIGRLFRRIEELQPVSATLPALVSDEEEEKERWLAAVRQAIASIRAGRLDKVVLARSRRLAFTGTIDAAAVLARLR
EQQPFAYLFAFEQDGRCFIGASPEQLVQKEGEMCQSVCLAGSVRRGSSPGEDEQLGAWLQADSKNREEHQFVVHMIRRVF
SSVCETVEMPTAPQLLKLPHIQHLCTPVVGRGCQEPSVLRLVEMMHPTPALGGTPRERAMEAIRSLEPLDRGWYAAPIGW
VDADGNGEWAVAIRSGLLDVDKAMLFAGCGIVAHSDPTSEYEETNVKMAPMLSSLGVMSND

Sequences:

>Translated_461_residues
MRVVAIVSQHKIQQQLHELAANAARPFVSWTEQLEDIDPVSFFMQGPSCGFRERFFWSDRSGSTIFVGLGSAYTIETEET
DGRFHYVEQQRQRWVEQIERHGEADMPPLLFGGFAFDPHQPRTSVWRGFPAGKLIAPTVLLSVKNGRTLLTVTLPTDGVE
RDMERIGRLFRRIEELQPVSATLPALVSDEEEEKERWLAAVRQAIASIRAGRLDKVVLARSRRLAFTGTIDAAAVLARLR
EQQPFAYLFAFEQDGRCFIGASPEQLVQKEGEMCQSVCLAGSVRRGSSPGEDEQLGAWLQADSKNREEHQFVVHMIRRVF
SSVCETVEMPTAPQLLKLPHIQHLCTPVVGRGCQEPSVLRLVEMMHPTPALGGTPRERAMEAIRSLEPLDRGWYAAPIGW
VDADGNGEWAVAIRSGLLDVDKAMLFAGCGIVAHSDPTSEYEETNVKMAPMLSSLGVMSND
>Mature_461_residues
MRVVAIVSQHKIQQQLHELAANAARPFVSWTEQLEDIDPVSFFMQGPSCGFRERFFWSDRSGSTIFVGLGSAYTIETEET
DGRFHYVEQQRQRWVEQIERHGEADMPPLLFGGFAFDPHQPRTSVWRGFPAGKLIAPTVLLSVKNGRTLLTVTLPTDGVE
RDMERIGRLFRRIEELQPVSATLPALVSDEEEEKERWLAAVRQAIASIRAGRLDKVVLARSRRLAFTGTIDAAAVLARLR
EQQPFAYLFAFEQDGRCFIGASPEQLVQKEGEMCQSVCLAGSVRRGSSPGEDEQLGAWLQADSKNREEHQFVVHMIRRVF
SSVCETVEMPTAPQLLKLPHIQHLCTPVVGRGCQEPSVLRLVEMMHPTPALGGTPRERAMEAIRSLEPLDRGWYAAPIGW
VDADGNGEWAVAIRSGLLDVDKAMLFAGCGIVAHSDPTSEYEETNVKMAPMLSSLGVMSND

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=258, Percent_Identity=39.5348837209302, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI87082077, Length=407, Percent_Identity=30.2211302211302, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788114, Length=264, Percent_Identity=25.3787878787879, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787518, Length=310, Percent_Identity=25.1612903225806, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=239, Percent_Identity=31.7991631799163, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51411; Mature: 51411

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVAIVSQHKIQQQLHELAANAARPFVSWTEQLEDIDPVSFFMQGPSCGFRERFFWSDR
CEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCCCHHEEECCC
SGSTIFVGLGSAYTIETEETDGRFHYVEQQRQRWVEQIERHGEADMPPLLFGGFAFDPHQ
CCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
PRTSVWRGFPAGKLIAPTVLLSVKNGRTLLTVTLPTDGVERDMERIGRLFRRIEELQPVS
CHHHHHCCCCCCCHHHCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHH
ATLPALVSDEEEEKERWLAAVRQAIASIRAGRLDKVVLARSRRLAFTGTIDAAAVLARLR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCHHHHHHHHHHH
EQQPFAYLFAFEQDGRCFIGASPEQLVQKEGEMCQSVCLAGSVRRGSSPGEDEQLGAWLQ
HCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
ADSKNREEHQFVVHMIRRVFSSVCETVEMPTAPQLLKLPHIQHLCTPVVGRGCQEPSVLR
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCHHHHH
LVEMMHPTPALGGTPRERAMEAIRSLEPLDRGWYAAPIGWVDADGNGEWAVAIRSGLLDV
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCEEECCCCCCEEEEEHHCCHHH
DKAMLFAGCGIVAHSDPTSEYEETNVKMAPMLSSLGVMSND
HHHHHHHCCCEEECCCCCCCHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MRVVAIVSQHKIQQQLHELAANAARPFVSWTEQLEDIDPVSFFMQGPSCGFRERFFWSDR
CEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCCCHHEEECCC
SGSTIFVGLGSAYTIETEETDGRFHYVEQQRQRWVEQIERHGEADMPPLLFGGFAFDPHQ
CCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
PRTSVWRGFPAGKLIAPTVLLSVKNGRTLLTVTLPTDGVERDMERIGRLFRRIEELQPVS
CHHHHHCCCCCCCHHHCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHH
ATLPALVSDEEEEKERWLAAVRQAIASIRAGRLDKVVLARSRRLAFTGTIDAAAVLARLR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCHHHHHHHHHHH
EQQPFAYLFAFEQDGRCFIGASPEQLVQKEGEMCQSVCLAGSVRRGSSPGEDEQLGAWLQ
HCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
ADSKNREEHQFVVHMIRRVFSSVCETVEMPTAPQLLKLPHIQHLCTPVVGRGCQEPSVLR
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCHHHHH
LVEMMHPTPALGGTPRERAMEAIRSLEPLDRGWYAAPIGWVDADGNGEWAVAIRSGLLDV
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCEEECCCCCCEEEEEHHCCHHH
DKAMLFAGCGIVAHSDPTSEYEETNVKMAPMLSSLGVMSND
HHHHHHHCCCEEECCCCCCCHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]