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The map label for this gene is metK

Identifier: 138896387

GI number: 138896387

Start: 2867903

End: 2869117

Strand: Reverse

Name: metK

Synonym: GTNG_2750

Alternate gene names: 138896387

Gene position: 2869117-2867903 (Counterclockwise)

Preceding gene: 138896389

Following gene: 138896385

Centisome position: 80.81

GC content: 53.25

Gene sequence:

>1215_bases
ATGTCAGCCAAACGCCGCTTGTTCACTTCAGAATCTGTAACAGAAGGGCATCCGGATAAAATTTGTGACCAAATTTCGGA
TGCCATTTTGGATGCCATTTTAGAAAAAGATCCGAACGCCCGTGTCGCTTGTGAAACGAGCGTCACAACAGGTCTCGTGC
TTGTTAGCGGGGAAATTACGACCTCGACGTACGTCGATATTCCGCGCATCGTTCGCGATACGGTCCGCGAGATCGGCTAT
ACGCGTGCGAAGTTCGGGTTTGACGCCGATACGTGCGCGGTGTTGACGGCCATTGACGAGCAGTCGCCGGATATCGCGAT
GGGGGTTGACCAGGCACTTGAGGCACGCGAAGGTCAGATGACTGACGAAGAAATTGAAGCGATCGGTGCCGGCGACCAAG
GGCTCATGTTTGGATTTGCCTGCAATGAAACGGAAGAGCTTATGCCGCTCCCGATTTCGCTTGCCCATCGTCTTTCGCGT
CGATTGGCCGAAGTGCGCAAAACGGATGTTTTGCCATACTTGCGTCCAGACGGCAAAACGCAAGTGACGATCGAATATGA
TGAAAACGGCAAACCGGTGCGCGTGGATACGATTGTCATTTCAACGCAGCATCATCCGGAAATTGAGCACGACCAAATCG
AACGCGATATGAAAGAACACGTCATCAAGCCGATTGTTCCGGCGGAGTTGTTGGATGAGAACACGAAATATTTCATCAAC
CCGACCGGCCGCTTCGTCATCGGTGGTCCGCAAGGGGACGCTGGACTGACAGGGCGGAAAATCATCGTTGACACGTACGG
TGGTTACGCTCGCCATGGCGGCGGTGCATTCTCGGGGAAAGACCCGACGAAAGTTGACCGTTCGGCAGCATATGCGGCTC
GCTATGTTGCGAAAAACATTGTTGCGGCCGGACTGGCCGACAAGTGCGAAGTGCAGCTTGCTTACGCCATCGGCGTCGCT
CGTCCGGTATCGATTTCGATTGATACGTTTGGCACCGGCAAAGTGTCGGAAGACGTTTTAATTGAAGTTGTCCGCAACAA
CTTCGATCTTCGCCCGGCTGGCATCATCAAAATGCTGGATCTGCGTCGTCCGATTTATAAACAAACAGCGGCTTACGGCC
ATTTCGGGCGCACGGATGTCGACTTGCCTTGGGAGCGCACGGATAAAGCAGCGGTGCTGAAAGAGCAAGCACTGGCATTA
GCGAAAAACGAATAA

Upstream 100 bases:

>100_bases
TTGCAATGACCTTTCCTCACCGTGACAGGAAAGGTTTTTTTATATGAAGGGAAACGAGTTCCGTATCGCCACATTATGTC
AAGAGGAGGAAATGAGCCGA

Downstream 100 bases:

>100_bases
ATGAAAAACGGAGGGGGACTGCACTAAGGTGAGTCTTCCTCCGTTTCTTTTTATATAGACATCGAAATGAAGCGTGCTGT
TGTTGGTTCTGTCCTTTTTA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSAKRRLFTSESVTEGHPDKICDQISDAILDAILEKDPNARVACETSVTTGLVLVSGEITTSTYVDIPRIVRDTVREIGY
TRAKFGFDADTCAVLTAIDEQSPDIAMGVDQALEAREGQMTDEEIEAIGAGDQGLMFGFACNETEELMPLPISLAHRLSR
RLAEVRKTDVLPYLRPDGKTQVTIEYDENGKPVRVDTIVISTQHHPEIEHDQIERDMKEHVIKPIVPAELLDENTKYFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKCEVQLAYAIGVA
RPVSISIDTFGTGKVSEDVLIEVVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLPWERTDKAAVLKEQALAL
AKNE

Sequences:

>Translated_404_residues
MSAKRRLFTSESVTEGHPDKICDQISDAILDAILEKDPNARVACETSVTTGLVLVSGEITTSTYVDIPRIVRDTVREIGY
TRAKFGFDADTCAVLTAIDEQSPDIAMGVDQALEAREGQMTDEEIEAIGAGDQGLMFGFACNETEELMPLPISLAHRLSR
RLAEVRKTDVLPYLRPDGKTQVTIEYDENGKPVRVDTIVISTQHHPEIEHDQIERDMKEHVIKPIVPAELLDENTKYFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKCEVQLAYAIGVA
RPVSISIDTFGTGKVSEDVLIEVVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLPWERTDKAAVLKEQALAL
AKNE
>Mature_403_residues
SAKRRLFTSESVTEGHPDKICDQISDAILDAILEKDPNARVACETSVTTGLVLVSGEITTSTYVDIPRIVRDTVREIGYT
RAKFGFDADTCAVLTAIDEQSPDIAMGVDQALEAREGQMTDEEIEAIGAGDQGLMFGFACNETEELMPLPISLAHRLSRR
LAEVRKTDVLPYLRPDGKTQVTIEYDENGKPVRVDTIVISTQHHPEIEHDQIERDMKEHVIKPIVPAELLDENTKYFINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKCEVQLAYAIGVAR
PVSISIDTFGTGKVSEDVLIEVVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLPWERTDKAAVLKEQALALA
KNE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=60.4712041884817, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=60.2094240837696, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1789311, Length=393, Percent_Identity=66.412213740458, Blast_Score=532, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17538498, Length=407, Percent_Identity=55.5282555282555, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=56.0411311053985, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25145633, Length=398, Percent_Identity=55.2763819095477, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=54.2416452442159, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=51.9280205655527, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565911, Length=356, Percent_Identity=53.6516853932584, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25145635, Length=347, Percent_Identity=53.0259365994236, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=55.3054662379421, Blast_Score=337, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI32565909, Length=80, Percent_Identity=58.75, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=72.7272727272727, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=56.9230769230769, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=57.1801566579634, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45552159, Length=382, Percent_Identity=57.0680628272251, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580529, Length=382, Percent_Identity=57.0680628272251, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580537, Length=382, Percent_Identity=57.0680628272251, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580533, Length=382, Percent_Identity=57.0680628272251, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580535, Length=382, Percent_Identity=57.0680628272251, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580531, Length=382, Percent_Identity=57.0680628272251, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580527, Length=382, Percent_Identity=56.5445026178011, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580525, Length=382, Percent_Identity=56.5445026178011, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=55.4054054054054, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=55.9055118110236, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_GEOTN (A4IRZ1)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126840.1
- ProteinModelPortal:   A4IRZ1
- SMR:   A4IRZ1
- STRING:   A4IRZ1
- GeneID:   4967676
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2750
- NMPDR:   fig|420246.5.peg.2654
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   A4IRZ1
- ProtClustDB:   PRK05250
- BioCyc:   GTHE420246:GTNG_2750-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44259; Mature: 44127

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKRRLFTSESVTEGHPDKICDQISDAILDAILEKDPNARVACETSVTTGLVLVSGEIT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCC
TSTYVDIPRIVRDTVREIGYTRAKFGFDADTCAVLTAIDEQSPDIAMGVDQALEAREGQM
CCHHCCHHHHHHHHHHHCCCHHHHCCCCCCHHEEEEECCCCCCCHHHCHHHHHHHHCCCC
TDEEIEAIGAGDQGLMFGFACNETEELMPLPISLAHRLSRRLAEVRKTDVLPYLRPDGKT
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCE
QVTIEYDENGKPVRVDTIVISTQHHPEIEHDQIERDMKEHVIKPIVPAELLDENTKYFIN
EEEEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VAAGLADKCEVQLAYAIGVARPVSISIDTFGTGKVSEDVLIEVVRNNFDLRPAGIIKMLD
HHHCCCCHHHEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLPWERTDKAAVLKEQALALAKNE
HCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAKRRLFTSESVTEGHPDKICDQISDAILDAILEKDPNARVACETSVTTGLVLVSGEIT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCC
TSTYVDIPRIVRDTVREIGYTRAKFGFDADTCAVLTAIDEQSPDIAMGVDQALEAREGQM
CCHHCCHHHHHHHHHHHCCCHHHHCCCCCCHHEEEEECCCCCCCHHHCHHHHHHHHCCCC
TDEEIEAIGAGDQGLMFGFACNETEELMPLPISLAHRLSRRLAEVRKTDVLPYLRPDGKT
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCE
QVTIEYDENGKPVRVDTIVISTQHHPEIEHDQIERDMKEHVIKPIVPAELLDENTKYFIN
EEEEEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VAAGLADKCEVQLAYAIGVARPVSISIDTFGTGKVSEDVLIEVVRNNFDLRPAGIIKMLD
HHHCCCCHHHEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLPWERTDKAAVLKEQALALAKNE
HCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA