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The map label for this gene is ytzG [H]

Identifier: 138896374

GI number: 138896374

Start: 2854466

End: 2855212

Strand: Reverse

Name: ytzG [H]

Synonym: GTNG_2737

Alternate gene names: 138896374

Gene position: 2855212-2854466 (Counterclockwise)

Preceding gene: 138896375

Following gene: 138896373

Centisome position: 80.42

GC content: 53.28

Gene sequence:

>747_bases
GTGGAACTGCGCATTGACAAGCTGCTCGCCCATATGGGCTATGGGACGAGGAAGGAAGTAAAAAAGCTGCTGAAGTCCGG
CTTGGTGAAAGTGGATGGCGCTGCTGTCTATGATCCGAAAACGAAAGTGCGGCCGGACGAGCAGACGGTGACCGTCTGGG
GAGAAACGGTCGAGTATAAACAGTTCATTTATTTGATGATGAACAAGCCCCCCGGCGTCATCTCAGCGACGGAAGACGCT
GTGGAAGAGACGGTCGTTGATTTGCTTGAAGAGGAAGATCGGCTGTTTGACCCGTTTCCAGTCGGGCGGCTCGACAAAGA
CACGGAAGGGCTTCTGTTGTTGACGAATGATGGACAGCTCGCCCATCAGCTGTTGGCGCCGAAAAAACATGTGCCGAAAA
CGTACTTTGCCGTCATTGATGGCGAGGTGACGGAAGACGATGTGGAGGCGTTTCGGCGCGGTGTGGTGCTCGATGACGGC
TATGAGACGAAGCCGGCCGAGCTTGTCGTGTTGAAATCCGGCCTGCGTTCCGATGTCGAAGTGACAATTACAGAAGGGAA
ATTTCATCAGGTGAAACGGATGTTTCAAGCGGTCGGCAAGCGGGTCGTTTATTTGAAACGGGTGCAAATGGGGCCGCTTT
CTCTCGATCCGAATTTAGCGGTTGGCGAGTATCGGGAATTGACAGATGAAGAGGTGGAGATGCTGAAGCAATATCGGGCG
GACGCGGATGAAAAAAAAGGAACCTGA

Upstream 100 bases:

>100_bases
CACATCTCCTTTCTGTATCTTTCCCTCATAGTGTATGGTGAGGAGGCAAAAGCGGACTAGATAAACGCCCAAATAGAGGA
ATATTTTAGGAGGGAGTTTC

Downstream 100 bases:

>100_bases
GACGGTTCCTTTTTTATGAAAAAGTGAAGAATATTCGTTCGTTATGAAAAGACATTCTGCATTCATCTCTCATCCTGTTT
TGCCCTGTTGGCACCGCTCA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MELRIDKLLAHMGYGTRKEVKKLLKSGLVKVDGAAVYDPKTKVRPDEQTVTVWGETVEYKQFIYLMMNKPPGVISATEDA
VEETVVDLLEEEDRLFDPFPVGRLDKDTEGLLLLTNDGQLAHQLLAPKKHVPKTYFAVIDGEVTEDDVEAFRRGVVLDDG
YETKPAELVVLKSGLRSDVEVTITEGKFHQVKRMFQAVGKRVVYLKRVQMGPLSLDPNLAVGEYRELTDEEVEMLKQYRA
DADEKKGT

Sequences:

>Translated_248_residues
MELRIDKLLAHMGYGTRKEVKKLLKSGLVKVDGAAVYDPKTKVRPDEQTVTVWGETVEYKQFIYLMMNKPPGVISATEDA
VEETVVDLLEEEDRLFDPFPVGRLDKDTEGLLLLTNDGQLAHQLLAPKKHVPKTYFAVIDGEVTEDDVEAFRRGVVLDDG
YETKPAELVVLKSGLRSDVEVTITEGKFHQVKRMFQAVGKRVVYLKRVQMGPLSLDPNLAVGEYRELTDEEVEMLKQYRA
DADEKKGT
>Mature_248_residues
MELRIDKLLAHMGYGTRKEVKKLLKSGLVKVDGAAVYDPKTKVRPDEQTVTVWGETVEYKQFIYLMMNKPPGVISATEDA
VEETVVDLLEEEDRLFDPFPVGRLDKDTEGLLLLTNDGQLAHQLLAPKKHVPKTYFAVIDGEVTEDDVEAFRRGVVLDDG
YETKPAELVVLKSGLRSDVEVTITEGKFHQVKRMFQAVGKRVVYLKRVQMGPLSLDPNLAVGEYRELTDEEVEMLKQYRA
DADEKKGT

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=38.0952380952381, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=33.7349397590361, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=29.7872340425532, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=37.0165745856354, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 28013; Mature: 28013

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRIDKLLAHMGYGTRKEVKKLLKSGLVKVDGAAVYDPKTKVRPDEQTVTVWGETVEYK
CCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCEEECCCCCCCCCCCEEEEECCHHHHE
QFIYLMMNKPPGVISATEDAVEETVVDLLEEEDRLFDPFPVGRLDKDTEGLLLLTNDGQL
EEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHH
AHQLLAPKKHVPKTYFAVIDGEVTEDDVEAFRRGVVLDDGYETKPAELVVLKSGLRSDVE
HHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCE
VTITEGKFHQVKRMFQAVGKRVVYLKRVQMGPLSLDPNLAVGEYRELTDEEVEMLKQYRA
EEEECCHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHC
DADEKKGT
CCCCCCCC
>Mature Secondary Structure
MELRIDKLLAHMGYGTRKEVKKLLKSGLVKVDGAAVYDPKTKVRPDEQTVTVWGETVEYK
CCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCEEECCCCCCCCCCCEEEEECCHHHHE
QFIYLMMNKPPGVISATEDAVEETVVDLLEEEDRLFDPFPVGRLDKDTEGLLLLTNDGQL
EEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHH
AHQLLAPKKHVPKTYFAVIDGEVTEDDVEAFRRGVVLDDGYETKPAELVVLKSGLRSDVE
HHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCE
VTITEGKFHQVKRMFQAVGKRVVYLKRVQMGPLSLDPNLAVGEYRELTDEEVEMLKQYRA
EEEECCHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHC
DADEKKGT
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]