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The map label for this gene is ligT [C]

Identifier: 138896370

GI number: 138896370

Start: 2850631

End: 2851209

Strand: Reverse

Name: ligT [C]

Synonym: GTNG_2733

Alternate gene names: 138896370

Gene position: 2851209-2850631 (Counterclockwise)

Preceding gene: 138896371

Following gene: 138896369

Centisome position: 80.31

GC content: 55.96

Gene sequence:

>579_bases
ATGAAACGCAACCATTATTTTATTGCCGTTCCGCTGACGGCGGAGGCGAAACAAGCGATTGCCCGTTTTGCGGGCGATAT
GGCATCATCGCTTCCGTTCCGTACATGGGTGCATGAACAAGACTACCATATTACCCTTGCATTTTTAGGGGATGTGCCGC
CACAGAAAATGGACCCGCTATGTGAAAAGGTAGCGGCAGCCGCAGCCCGCTACGCTCCGTTTTCCCTTCAACTTGACGGG
CTCGGAACGTTCGGAGAGCGCACAGCGCCCCGCATTTTTTGGCAAGGAGTCAAAGCGGAGGAAACGCTCAATGCGCTGCG
GCGCGATGTGTATGAGGCTTGCTTAACGCTCGGTTTTTCTCTCGACCGGCGGCCGTTTGCCCCGCATATTACGATCGCTC
GCAAATGGCAAGGCGCCGAGCCGTTTAAGCCGGAAATGCTCGGTATGCTTCCGGCGGCTTCGGCGGTGTTTTCGGTGCCG
GAGATTGTGCTATACCGGACAAATATGCACCAGACGCCAAAATATGAAGTGATCGCCTCGTTTCCGCTGCTGGGTGCACC
GGCGGAGGGCAACCAATAG

Upstream 100 bases:

>100_bases
CGGTTTATTTGTTTTTTGGCGCTTGCACGCTCGCTGGCGTGTTCGTATTGCTTTGGCTTGCCAAACTAGAACGAGACGAC
CAATCGGCAGGTGAGAGAGG

Downstream 100 bases:

>100_bases
TAGGTGAGGGTATGGGACAGCTTGTGAAAATGCGGGATTGTATCTCCCGCTATGAAACGGATGTGTATTATTATGTGCCG
GAATTTATCCGGCTGAAACA

Product: malA gene products

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKRNHYFIAVPLTAEAKQAIARFAGDMASSLPFRTWVHEQDYHITLAFLGDVPPQKMDPLCEKVAAAAARYAPFSLQLDG
LGTFGERTAPRIFWQGVKAEETLNALRRDVYEACLTLGFSLDRRPFAPHITIARKWQGAEPFKPEMLGMLPAASAVFSVP
EIVLYRTNMHQTPKYEVIASFPLLGAPAEGNQ

Sequences:

>Translated_192_residues
MKRNHYFIAVPLTAEAKQAIARFAGDMASSLPFRTWVHEQDYHITLAFLGDVPPQKMDPLCEKVAAAAARYAPFSLQLDG
LGTFGERTAPRIFWQGVKAEETLNALRRDVYEACLTLGFSLDRRPFAPHITIARKWQGAEPFKPEMLGMLPAASAVFSVP
EIVLYRTNMHQTPKYEVIASFPLLGAPAEGNQ
>Mature_192_residues
MKRNHYFIAVPLTAEAKQAIARFAGDMASSLPFRTWVHEQDYHITLAFLGDVPPQKMDPLCEKVAAAAARYAPFSLQLDG
LGTFGERTAPRIFWQGVKAEETLNALRRDVYEACLTLGFSLDRRPFAPHITIARKWQGAEPFKPEMLGMLPAASAVFSVP
EIVLYRTNMHQTPKYEVIASFPLLGAPAEGNQ

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21392; Mature: 21392

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNHYFIAVPLTAEAKQAIARFAGDMASSLPFRTWVHEQDYHITLAFLGDVPPQKMDPL
CCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEEECCCCCHHCCHH
CEKVAAAAARYAPFSLQLDGLGTFGERTAPRIFWQGVKAEETLNALRRDVYEACLTLGFS
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
LDRRPFAPHITIARKWQGAEPFKPEMLGMLPAASAVFSVPEIVLYRTNMHQTPKYEVIAS
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEC
FPLLGAPAEGNQ
CCCCCCCCCCCC
>Mature Secondary Structure
MKRNHYFIAVPLTAEAKQAIARFAGDMASSLPFRTWVHEQDYHITLAFLGDVPPQKMDPL
CCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEEECCCCCHHCCHH
CEKVAAAAARYAPFSLQLDGLGTFGERTAPRIFWQGVKAEETLNALRRDVYEACLTLGFS
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
LDRRPFAPHITIARKWQGAEPFKPEMLGMLPAASAVFSVPEIVLYRTNMHQTPKYEVIAS
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEC
FPLLGAPAEGNQ
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8190087 [H]