| Definition | Geobacillus thermodenitrificans NG80-2 chromosome, complete genome. |
|---|---|
| Accession | NC_009328 |
| Length | 3,550,319 |
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The map label for this gene is pbpF [H]
Identifier: 138896347
GI number: 138896347
Start: 2823809
End: 2826568
Strand: Direct
Name: pbpF [H]
Synonym: GTNG_2710
Alternate gene names: 138896347
Gene position: 2823809-2826568 (Clockwise)
Preceding gene: 138896341
Following gene: 138896349
Centisome position: 79.54
GC content: 51.99
Gene sequence:
>2760_bases ATGGAACAAAAACGGTTTCGAGCTTCTTTACAAAAAGTGTGGTACTATTTTTCCCTTACATATGACGTCGTCTGGAATGT CATCCTTATTTTGCTCATCGCCACACTCTGTCTTGTTTGTTTTGCCGTGGGCATCGGTGCCGGCTATTTTGCCTTTTTGG TGAAAGATATGGAGGTTCCGTCCCATGACCAACTGAAAAATGAGATTTACAGCTATGAAGAAACGAGCCATATGTATTTT GCTGACGGCATCTATTTAGGCCAATTCCGATCTGATCTCGACCGCGAGTCGGTGCGCCTTGCCGACGTCTCCCCGTACGT TGTGCAGGCGGTGATCGCCACGGAAGATGAGCATTTTTACGAGCATGACGGCGTCGTTCCCAAGGCTGTCATTCGCGCTA TATTTCAAGAAGTGACCAATTCACCGATCAAAAGCGGCGGCAGTACACTTACGCAGCAACTCGTCAAAAACCAGCTGCTG ACCAGTGAAGTGTCGTTTGAACGCAAGGCGAAGGAAATGTTATTGGCGATGCGCTTGGAACGGTTTTTTTCCAAGGAAGA AATTTTAGAGGCCTACTTAAACGTTGTTCCGTTCGGCCGCAGTTCATCGGGCCGCAACATCGCCGGCATCCAAGCCGCTG CCCAAGGAGTGTTTGGCGTTGACGCCAAAGAACTCAATCTCGCCCAAGCGGCGTTTTTAGCCGGCTTGCCGCAAAACCCG TTCGTCTATACACCGTTTACCGCTGATGGGAGTCGCAAACAAGATATATCGGCCGGATTGGAACGGATGAAAACAGTGCT CTACCGGATGAAGCGCGCTGGTTATATTTCTGATAAGCAGTATAAAGAAGCGCTCGCCTACGATGTGACGAAGCATTTCG CCGCTCCGAAACCGACACCGTTTGAGCGCTATCCGTTTTTAACAATGGAAATCGAAAGACGAGCCAAAGACGTGCTTGCG GAAACGTTCGCTAAACGCGATGGCCACAAGCCGGAAGAGCTGAAACGCGATGCTGCTTTATACAGCCGCTATGTCGGTGA AGCGGAAAAAATGTTGCGGCAAGGAGGCTTGCACATCCATACGACGATCGACAAAGCGATCTATGAGCAAATGCAGCGTG TGGCCGCCAACTACCCGTATTACGGCAACGAGCGGTTGTATTATGTGACAGACACAAAAACAGGGAAACGGATCGCCAAG CGCCAGCCGGTCGAAGTCGGGGCGATGCTCATCGAAAACAAAACCGGCAAAATCATCAGCTTTGTCGGCGGCCGTGATTA CAAGCGCGAGCAGTTAAATCACGCCACCCAGGCGTACCGTTCGAACGGTTCAACGATGAAGCCGCTTTTAGTGTACGCAC CGGCGATGGAGATGGGCGTCATTCAGCCCGGCTCGATCATTCCTGATCTTCCGCTTTCGATCGGTTCGTATAAGCCAAAA AATGCGGATGGAAAAACGCACGGCCTTGTCACAGCGCGGCGCGCGCTTCAACATTCGTACAACATCCCGGCTGTTCGGGT CTATACGAAAATCCAAAACCGCCATCCGGTCGACTATTTGCACAAAATGGGGATTACCAGCGTCGCTAAGGAAGAAGAAA AACATCCGGCTTTAGCGATCGGCGGGACGAATATCGGTGTCACCGTCGAAGAAAACGTGAATGCGTATGCGACGTTTGCC AATTACGGCTCATTTGTCGATGCGTATATGATCGAAAAGATTACCGACAAAGACGGAAAAGTCATTTATCAACATACAAG CAAACCGGTTCGCGTCTTTTCTCCGCAAACATCGTATTTAATGATCGATATGATGCGTGACGTGCTGCGCGGTGGAACCG CCTCTTCAGTGCGGCGTTACTTGACGTTTTCTGCTGACTGGGCCGGCAAGACGGGAACATCGCAAGACAATCGCGATTCA TGGTTTGTTGCCACTAATCCGAACGTCACGTTCGGCGTTTGGATCGGTTACGATCGTCCAGCGTCGCTTGAGTCGCGCTA CAAAGGGTTTTCCTACGGCCAACGCACTCAGCTGTTATGGGCGCAATTGATGAATGCCGCCCATCGTGTGAAACCGAAAC TCATCAATCCGTCGGAACGGTTTTTAATGCCAGGCGGCATCGTTCGCCGCGCGTATTGTGCCACGAGTGGATTCAGCCCG TCATCGGCATGTAAAAAAGCTGGACTCGTTCGCTATGATTTATACAATACAAAGTTTACGCCGCGGCAAGGAAGCGATGA CTATTTGATCGAGGGTCGGGTTGTCGTCATTGGCGAGCATCGGTATGCAGCGCTCGAGACGACGCCGGAAGAGTTTGTCA CCCGCGAGGTCGTTGTCAATCCAAAATTGCTCGATCAGTGGGGAATCAATAGAAAGACATCGGTCGGCGATGCGTTCGTT GTTTCCACCTTAAAAGACAATGGACGCGTGCCCGCCCCACCGGCCGCCCGCCTGAATGACAATCAGCTCGTCTGGGCTCG CCACGGAGAGCGCGATGTCATCGGCTACCGCGTCTATCAGGTAAGCGAAACCGGAAAGCGGCAAATCGTCGCCATCATCA AAGCGGGGGAAACGACTTCCTTTTCCGATCTCAAACCGGGAGCAACATATTACGTCACCGCTGTCGATATTGCTGGCCGG GAATCAGCACCATCAAGCAAAGTCGCACTGCCGGCCGCCCAGTCCTCCCCGCCGGCCAATTCGCCCGCTAGTGCGGACAA CGCGGAAAATAGCGAGGCTCCCCCATCTTCTTCTAGATAA
Upstream 100 bases:
>100_bases TTTTTCGCCACAAATGGCGAAAAAGCGACAAAATCCCGCAACAGAACATGCGAGTATGCTATACTTTATATATATGATTT GCCATTATAGGGGGCCCGAT
Downstream 100 bases:
>100_bases ATATTATGAGGCTTTCCAACCAAACAGTTGGAAAGCCTTTTTTTATGAGCGGGCACCTATTTTTCGACCGGCAGGAGAAC CTCCTTTCGCCGCCTATATA
Product: penicillin-binding protein-like protein
Products: NA
Alternate protein names: PBP-1F; Penicillin-binding protein F [H]
Number of amino acids: Translated: 919; Mature: 919
Protein sequence:
>919_residues MEQKRFRASLQKVWYYFSLTYDVVWNVILILLIATLCLVCFAVGIGAGYFAFLVKDMEVPSHDQLKNEIYSYEETSHMYF ADGIYLGQFRSDLDRESVRLADVSPYVVQAVIATEDEHFYEHDGVVPKAVIRAIFQEVTNSPIKSGGSTLTQQLVKNQLL TSEVSFERKAKEMLLAMRLERFFSKEEILEAYLNVVPFGRSSSGRNIAGIQAAAQGVFGVDAKELNLAQAAFLAGLPQNP FVYTPFTADGSRKQDISAGLERMKTVLYRMKRAGYISDKQYKEALAYDVTKHFAAPKPTPFERYPFLTMEIERRAKDVLA ETFAKRDGHKPEELKRDAALYSRYVGEAEKMLRQGGLHIHTTIDKAIYEQMQRVAANYPYYGNERLYYVTDTKTGKRIAK RQPVEVGAMLIENKTGKIISFVGGRDYKREQLNHATQAYRSNGSTMKPLLVYAPAMEMGVIQPGSIIPDLPLSIGSYKPK NADGKTHGLVTARRALQHSYNIPAVRVYTKIQNRHPVDYLHKMGITSVAKEEEKHPALAIGGTNIGVTVEENVNAYATFA NYGSFVDAYMIEKITDKDGKVIYQHTSKPVRVFSPQTSYLMIDMMRDVLRGGTASSVRRYLTFSADWAGKTGTSQDNRDS WFVATNPNVTFGVWIGYDRPASLESRYKGFSYGQRTQLLWAQLMNAAHRVKPKLINPSERFLMPGGIVRRAYCATSGFSP SSACKKAGLVRYDLYNTKFTPRQGSDDYLIEGRVVVIGEHRYAALETTPEEFVTREVVVNPKLLDQWGINRKTSVGDAFV VSTLKDNGRVPAPPAARLNDNQLVWARHGERDVIGYRVYQVSETGKRQIVAIIKAGETTSFSDLKPGATYYVTAVDIAGR ESAPSSKVALPAAQSSPPANSPASADNAENSEAPPSSSR
Sequences:
>Translated_919_residues MEQKRFRASLQKVWYYFSLTYDVVWNVILILLIATLCLVCFAVGIGAGYFAFLVKDMEVPSHDQLKNEIYSYEETSHMYF ADGIYLGQFRSDLDRESVRLADVSPYVVQAVIATEDEHFYEHDGVVPKAVIRAIFQEVTNSPIKSGGSTLTQQLVKNQLL TSEVSFERKAKEMLLAMRLERFFSKEEILEAYLNVVPFGRSSSGRNIAGIQAAAQGVFGVDAKELNLAQAAFLAGLPQNP FVYTPFTADGSRKQDISAGLERMKTVLYRMKRAGYISDKQYKEALAYDVTKHFAAPKPTPFERYPFLTMEIERRAKDVLA ETFAKRDGHKPEELKRDAALYSRYVGEAEKMLRQGGLHIHTTIDKAIYEQMQRVAANYPYYGNERLYYVTDTKTGKRIAK RQPVEVGAMLIENKTGKIISFVGGRDYKREQLNHATQAYRSNGSTMKPLLVYAPAMEMGVIQPGSIIPDLPLSIGSYKPK NADGKTHGLVTARRALQHSYNIPAVRVYTKIQNRHPVDYLHKMGITSVAKEEEKHPALAIGGTNIGVTVEENVNAYATFA NYGSFVDAYMIEKITDKDGKVIYQHTSKPVRVFSPQTSYLMIDMMRDVLRGGTASSVRRYLTFSADWAGKTGTSQDNRDS WFVATNPNVTFGVWIGYDRPASLESRYKGFSYGQRTQLLWAQLMNAAHRVKPKLINPSERFLMPGGIVRRAYCATSGFSP SSACKKAGLVRYDLYNTKFTPRQGSDDYLIEGRVVVIGEHRYAALETTPEEFVTREVVVNPKLLDQWGINRKTSVGDAFV VSTLKDNGRVPAPPAARLNDNQLVWARHGERDVIGYRVYQVSETGKRQIVAIIKAGETTSFSDLKPGATYYVTAVDIAGR ESAPSSKVALPAAQSSPPANSPASADNAENSEAPPSSSR >Mature_919_residues MEQKRFRASLQKVWYYFSLTYDVVWNVILILLIATLCLVCFAVGIGAGYFAFLVKDMEVPSHDQLKNEIYSYEETSHMYF ADGIYLGQFRSDLDRESVRLADVSPYVVQAVIATEDEHFYEHDGVVPKAVIRAIFQEVTNSPIKSGGSTLTQQLVKNQLL TSEVSFERKAKEMLLAMRLERFFSKEEILEAYLNVVPFGRSSSGRNIAGIQAAAQGVFGVDAKELNLAQAAFLAGLPQNP FVYTPFTADGSRKQDISAGLERMKTVLYRMKRAGYISDKQYKEALAYDVTKHFAAPKPTPFERYPFLTMEIERRAKDVLA ETFAKRDGHKPEELKRDAALYSRYVGEAEKMLRQGGLHIHTTIDKAIYEQMQRVAANYPYYGNERLYYVTDTKTGKRIAK RQPVEVGAMLIENKTGKIISFVGGRDYKREQLNHATQAYRSNGSTMKPLLVYAPAMEMGVIQPGSIIPDLPLSIGSYKPK NADGKTHGLVTARRALQHSYNIPAVRVYTKIQNRHPVDYLHKMGITSVAKEEEKHPALAIGGTNIGVTVEENVNAYATFA NYGSFVDAYMIEKITDKDGKVIYQHTSKPVRVFSPQTSYLMIDMMRDVLRGGTASSVRRYLTFSADWAGKTGTSQDNRDS WFVATNPNVTFGVWIGYDRPASLESRYKGFSYGQRTQLLWAQLMNAAHRVKPKLINPSERFLMPGGIVRRAYCATSGFSP SSACKKAGLVRYDLYNTKFTPRQGSDDYLIEGRVVVIGEHRYAALETTPEEFVTREVVVNPKLLDQWGINRKTSVGDAFV VSTLKDNGRVPAPPAARLNDNQLVWARHGERDVIGYRVYQVSETGKRQIVAIIKAGETTSFSDLKPGATYYVTAVDIAGR ESAPSSKVALPAAQSSPPANSPASADNAENSEAPPSSSR
Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell membrane; Single-pass membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1788867, Length=635, Percent_Identity=26.4566929133858, Blast_Score=171, Evalue=2e-43, Organism=Escherichia coli, GI87082258, Length=300, Percent_Identity=32, Blast_Score=140, Evalue=3e-34, Organism=Escherichia coli, GI1786343, Length=140, Percent_Identity=42.1428571428571, Blast_Score=107, Evalue=3e-24, Organism=Escherichia coli, GI1789601, Length=157, Percent_Identity=35.031847133758, Blast_Score=86, Evalue=1e-17,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.- [C]
Molecular weight: Translated: 102659; Mature: 102659
Theoretical pI: Translated: 9.56; Mature: 9.56
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEQKRFRASLQKVWYYFSLTYDVVWNVILILLIATLCLVCFAVGIGAGYFAFLVKDMEVP CCHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC SHDQLKNEIYSYEETSHMYFADGIYLGQFRSDLDRESVRLADVSPYVVQAVIATEDEHFY CHHHHHHHHHCCCCCCCEEEECCEEEHHHHHCCCCCCEEEECCCHHHHEEEHCCCCCHHH EHDGVVPKAVIRAIFQEVTNSPIKSGGSTLTQQLVKNQLLTSEVSFERKAKEMLLAMRLE HCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH RFFSKEEILEAYLNVVPFGRSSSGRNIAGIQAAAQGVFGVDAKELNLAQAAFLAGLPQNP HHHCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC FVYTPFTADGSRKQDISAGLERMKTVLYRMKRAGYISDKQYKEALAYDVTKHFAAPKPTP EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC FERYPFLTMEIERRAKDVLAETFAKRDGHKPEELKRDAALYSRYVGEAEKMLRQGGLHIH CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE TTIDKAIYEQMQRVAANYPYYGNERLYYVTDTKTGKRIAKRQPVEVGAMLIENKTGKIIS EHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHEEEECCCCCEEE FVGGRDYKREQLNHATQAYRSNGSTMKPLLVYAPAMEMGVIQPGSIIPDLPLSIGSYKPK EECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCC NADGKTHGLVTARRALQHSYNIPAVRVYTKIQNRHPVDYLHKMGITSVAKEEEKHPALAI CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCEEEE GGTNIGVTVEENVNAYATFANYGSFVDAYMIEKITDKDGKVIYQHTSKPVRVFSPQTSYL CCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCHH MIDMMRDVLRGGTASSVRRYLTFSADWAGKTGTSQDNRDSWFVATNPNVTFGVWIGYDRP HHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCC ASLESRYKGFSYGQRTQLLWAQLMNAAHRVKPKLINPSERFLMPGGIVRRAYCATSGFSP CCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHCCCCCH SSACKKAGLVRYDLYNTKFTPRQGSDDYLIEGRVVVIGEHRYAALETTPEEFVTREVVVN HHHHHHCCCEEEEEECCEECCCCCCCCEEEECEEEEEECCCEEEECCCHHHHHHHHHEEC PKLLDQWGINRKTSVGDAFVVSTLKDNGRVPAPPAARLNDNQLVWARHGERDVIGYRVYQ CHHHHHCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEE VSETGKRQIVAIIKAGETTSFSDLKPGATYYVTAVDIAGRESAPSSKVALPAAQSSPPAN ECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCCCC SPASADNAENSEAPPSSSR CCCCCCCCCCCCCCCCCCC >Mature Secondary Structure MEQKRFRASLQKVWYYFSLTYDVVWNVILILLIATLCLVCFAVGIGAGYFAFLVKDMEVP CCHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC SHDQLKNEIYSYEETSHMYFADGIYLGQFRSDLDRESVRLADVSPYVVQAVIATEDEHFY CHHHHHHHHHCCCCCCCEEEECCEEEHHHHHCCCCCCEEEECCCHHHHEEEHCCCCCHHH EHDGVVPKAVIRAIFQEVTNSPIKSGGSTLTQQLVKNQLLTSEVSFERKAKEMLLAMRLE HCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH RFFSKEEILEAYLNVVPFGRSSSGRNIAGIQAAAQGVFGVDAKELNLAQAAFLAGLPQNP HHHCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC FVYTPFTADGSRKQDISAGLERMKTVLYRMKRAGYISDKQYKEALAYDVTKHFAAPKPTP EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC FERYPFLTMEIERRAKDVLAETFAKRDGHKPEELKRDAALYSRYVGEAEKMLRQGGLHIH CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE TTIDKAIYEQMQRVAANYPYYGNERLYYVTDTKTGKRIAKRQPVEVGAMLIENKTGKIIS EHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHEEEECCCCCEEE FVGGRDYKREQLNHATQAYRSNGSTMKPLLVYAPAMEMGVIQPGSIIPDLPLSIGSYKPK EECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCC NADGKTHGLVTARRALQHSYNIPAVRVYTKIQNRHPVDYLHKMGITSVAKEEEKHPALAI CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCEEEE GGTNIGVTVEENVNAYATFANYGSFVDAYMIEKITDKDGKVIYQHTSKPVRVFSPQTSYL CCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCHH MIDMMRDVLRGGTASSVRRYLTFSADWAGKTGTSQDNRDSWFVATNPNVTFGVWIGYDRP HHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCC ASLESRYKGFSYGQRTQLLWAQLMNAAHRVKPKLINPSERFLMPGGIVRRAYCATSGFSP CCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHCCCCCH SSACKKAGLVRYDLYNTKFTPRQGSDDYLIEGRVVVIGEHRYAALETTPEEFVTREVVVN HHHHHHCCCEEEEEECCEECCCCCCCCEEEECEEEEEECCCEEEECCCHHHHHHHHHEEC PKLLDQWGINRKTSVGDAFVVSTLKDNGRVPAPPAARLNDNQLVWARHGERDVIGYRVYQ CHHHHHCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEE VSETGKRQIVAIIKAGETTSFSDLKPGATYYVTAVDIAGRESAPSSKVALPAAQSSPPAN ECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCCCC SPASADNAENSEAPPSSSR CCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9579061; 9384377; 8335642; 1459957 [H]