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The map label for this gene is hyfR [H]

Identifier: 138896345

GI number: 138896345

Start: 2819630

End: 2821090

Strand: Reverse

Name: hyfR [H]

Synonym: GTNG_2708

Alternate gene names: 138896345

Gene position: 2821090-2819630 (Counterclockwise)

Preceding gene: 138896346

Following gene: 138896344

Centisome position: 79.46

GC content: 31.14

Gene sequence:

>1461_bases
ATGGTTCCAGACCTTAAACATATCAATCGGTTTCCAATGAATAATCAACATATGCTACAGCATGAATGTGTTTACAAAAT
AGCATTAGATCATTTATACGATGGTGTTTTCATTACAGACAACGAATGTAAAATTCTTTACGTTAATGAAGCGTATACAA
AAATGTCAGGTCTTAAATATGAAGATGTTGTCGGCCATTATGTGGATGATTTAGAAGCAAAAGGTATATTTAAAAATTCT
ACTTCACCTCATGTTCGAAGGGAAAACAAGATTATTAGTTCCATTGGAAAGGCAAAAGATGGAGTAGAAATGTTAATTAC
AGGAAAACCGATATATGATGAAAACAATGAACTCATCTATGTAGTGGTTAATCAGAGGGATATTTCTGATTTAGAAATTA
TGAGGCAAAAATTAATAGTAACAGAAAACAAAATGGCAGTAGTTGAAAAAGAAAGAAAAAAGAAGGAAGAAGAAAACGAA
TATTATAAAAAACAATTTTTCAATAATACTGGGATTATTGGAGATAGCGAAGAGATTCGTGATGTAATCTATTTAATTAA
TAAAATTGCTCCACTAGATGTTACTGTTTTAATTACTGGAGAGAGCGGTGTAGGAAAAGAAGTCATTGCAAATGAAATTT
TCCAAAAGAGCTCGAGGAAGAATAAGCAGTTTATTAAAGTGAATTGTGCTGCAATTCCTACTAATTTGTTAGAATCTGAG
CTTTTTGGTTATGAAAAGGGGGCTTTTACTGGTGCAGACAAAGCCAAAAAAGGACTTTTTCAACTCGCAGATGGTGGTAC
CATTTTTTTAGATGAGATAGGGGACATTCCATTAGAAATTCAACCAAAATTACTGAGGGCTCTGCAACAAAAAGAGGTTG
TTCCTGTAGGTGGAACCCAAATTCAAAAATTAGATGTTCGAATTATTGCAGCAACAAATAAAGATTTGTCTCAACAAGTA
AAAGAGGGAAAGTTTCGCAAAGATTTATATTACCGATTAAATGTTTTTCCTATTGAAATTCCGCCCTTAAGGGAGAGGAA
AAGAGATATATATGCCTTAGGTGAATACTTTTTAAAACAATATAATAAAAAGTATGGAAAATCAGTGGTTTTATCTTCCG
AGGCATATTCATTGTTGATGAATTATGATTGGCCGGGTAATGTGAGGGAGTTACAGAATATTATTGAAAGATTAATTATT
ATTTCTGATGACCAAACAGTGATTAATAAAGATCAAATTGAGAAAATTATGTTTCCAAACACAATGTTAGATATCACAAA
ATCCGAAAAAGGACTAAAAGAAATTATTCAAGAGATTGAAAAAGAAATATTAATAGATGTATTAAAGAATACGGGAACTA
CTAGAAAAGCCGCAAAAAAATTAAAAATAGACCAATCCACATTAGTAAAAAAGCTAAAGAAATATGGAATAAATATTGAT
GAAATACGTCATCAAAGCTGA

Upstream 100 bases:

>100_bases
TATAAGATTAAGAAAATTAATATTATTACTAAAATAAATGGTATAATATTGTCCAAGAGAATGCACATTAGAATAAATTG
ATTAAAAGTGGGGGATGTAA

Downstream 100 bases:

>100_bases
TGATTCTTTTCATAATGAAAACGATACCATAAAAGCTATGAATATATGTAAAAAGTCCCCTGTTTTACATAATAGAAAAA
TAAGTGATGAAATATTTCAT

Product: sigma-54-dependent transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MVPDLKHINRFPMNNQHMLQHECVYKIALDHLYDGVFITDNECKILYVNEAYTKMSGLKYEDVVGHYVDDLEAKGIFKNS
TSPHVRRENKIISSIGKAKDGVEMLITGKPIYDENNELIYVVVNQRDISDLEIMRQKLIVTENKMAVVEKERKKKEEENE
YYKKQFFNNTGIIGDSEEIRDVIYLINKIAPLDVTVLITGESGVGKEVIANEIFQKSSRKNKQFIKVNCAAIPTNLLESE
LFGYEKGAFTGADKAKKGLFQLADGGTIFLDEIGDIPLEIQPKLLRALQQKEVVPVGGTQIQKLDVRIIAATNKDLSQQV
KEGKFRKDLYYRLNVFPIEIPPLRERKRDIYALGEYFLKQYNKKYGKSVVLSSEAYSLLMNYDWPGNVRELQNIIERLII
ISDDQTVINKDQIEKIMFPNTMLDITKSEKGLKEIIQEIEKEILIDVLKNTGTTRKAAKKLKIDQSTLVKKLKKYGINID
EIRHQS

Sequences:

>Translated_486_residues
MVPDLKHINRFPMNNQHMLQHECVYKIALDHLYDGVFITDNECKILYVNEAYTKMSGLKYEDVVGHYVDDLEAKGIFKNS
TSPHVRRENKIISSIGKAKDGVEMLITGKPIYDENNELIYVVVNQRDISDLEIMRQKLIVTENKMAVVEKERKKKEEENE
YYKKQFFNNTGIIGDSEEIRDVIYLINKIAPLDVTVLITGESGVGKEVIANEIFQKSSRKNKQFIKVNCAAIPTNLLESE
LFGYEKGAFTGADKAKKGLFQLADGGTIFLDEIGDIPLEIQPKLLRALQQKEVVPVGGTQIQKLDVRIIAATNKDLSQQV
KEGKFRKDLYYRLNVFPIEIPPLRERKRDIYALGEYFLKQYNKKYGKSVVLSSEAYSLLMNYDWPGNVRELQNIIERLII
ISDDQTVINKDQIEKIMFPNTMLDITKSEKGLKEIIQEIEKEILIDVLKNTGTTRKAAKKLKIDQSTLVKKLKKYGINID
EIRHQS
>Mature_486_residues
MVPDLKHINRFPMNNQHMLQHECVYKIALDHLYDGVFITDNECKILYVNEAYTKMSGLKYEDVVGHYVDDLEAKGIFKNS
TSPHVRRENKIISSIGKAKDGVEMLITGKPIYDENNELIYVVVNQRDISDLEIMRQKLIVTENKMAVVEKERKKKEEENE
YYKKQFFNNTGIIGDSEEIRDVIYLINKIAPLDVTVLITGESGVGKEVIANEIFQKSSRKNKQFIKVNCAAIPTNLLESE
LFGYEKGAFTGADKAKKGLFQLADGGTIFLDEIGDIPLEIQPKLLRALQQKEVVPVGGTQIQKLDVRIIAATNKDLSQQV
KEGKFRKDLYYRLNVFPIEIPPLRERKRDIYALGEYFLKQYNKKYGKSVVLSSEAYSLLMNYDWPGNVRELQNIIERLII
ISDDQTVINKDQIEKIMFPNTMLDITKSEKGLKEIIQEIEKEILIDVLKNTGTTRKAAKKLKIDQSTLVKKLKKYGINID
EIRHQS

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=41.812865497076, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1789233, Length=468, Percent_Identity=33.974358974359, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1788550, Length=313, Percent_Identity=45.6869009584665, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1789087, Length=367, Percent_Identity=40.5994550408719, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI1790437, Length=306, Percent_Identity=44.4444444444444, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1790299, Length=361, Percent_Identity=39.0581717451524, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=39.2523364485981, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788905, Length=230, Percent_Identity=47.8260869565217, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=38.871473354232, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=39.6923076923077, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=36.4217252396166, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1789828, Length=348, Percent_Identity=29.0229885057471, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=29.8076923076923, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55846; Mature: 55846

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPDLKHINRFPMNNQHMLQHECVYKIALDHLYDGVFITDNECKILYVNEAYTKMSGLKY
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEEHHHHHHCCCCH
EDVVGHYVDDLEAKGIFKNSTSPHVRRENKIISSIGKAKDGVEMLITGKPIYDENNELIY
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEE
VVVNQRDISDLEIMRQKLIVTENKMAVVEKERKKKEEENEYYKKQFFNNTGIIGDSEEIR
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
DVIYLINKIAPLDVTVLITGESGVGKEVIANEIFQKSSRKNKQFIKVNCAAIPTNLLESE
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHHHHHH
LFGYEKGAFTGADKAKKGLFQLADGGTIFLDEIGDIPLEIQPKLLRALQQKEVVPVGGTQ
HCCCCCCCCCCCHHHHHCCEEECCCCEEEEECCCCCCCEECHHHHHHHHHCCCCCCCCCE
IQKLDVRIIAATNKDLSQQVKEGKFRKDLYYRLNVFPIEIPPLRERKRDIYALGEYFLKQ
EEEEEEEEEEECCHHHHHHHHCCCHHHCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
YNKKYGKSVVLSSEAYSLLMNYDWPGNVRELQNIIERLIIISDDQTVINKDQIEKIMFPN
HHHHHCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCC
TMLDITKSEKGLKEIIQEIEKEILIDVLKNTGTTRKAAKKLKIDQSTLVKKLKKYGINID
HHEEHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHH
EIRHQS
HHCCCC
>Mature Secondary Structure
MVPDLKHINRFPMNNQHMLQHECVYKIALDHLYDGVFITDNECKILYVNEAYTKMSGLKY
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEEHHHHHHCCCCH
EDVVGHYVDDLEAKGIFKNSTSPHVRRENKIISSIGKAKDGVEMLITGKPIYDENNELIY
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEE
VVVNQRDISDLEIMRQKLIVTENKMAVVEKERKKKEEENEYYKKQFFNNTGIIGDSEEIR
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
DVIYLINKIAPLDVTVLITGESGVGKEVIANEIFQKSSRKNKQFIKVNCAAIPTNLLESE
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHHHHHH
LFGYEKGAFTGADKAKKGLFQLADGGTIFLDEIGDIPLEIQPKLLRALQQKEVVPVGGTQ
HCCCCCCCCCCCHHHHHCCEEECCCCEEEEECCCCCCCEECHHHHHHHHHCCCCCCCCCE
IQKLDVRIIAATNKDLSQQVKEGKFRKDLYYRLNVFPIEIPPLRERKRDIYALGEYFLKQ
EEEEEEEEEEECCHHHHHHHHCCCHHHCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
YNKKYGKSVVLSSEAYSLLMNYDWPGNVRELQNIIERLIIISDDQTVINKDQIEKIMFPN
HHHHHCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCC
TMLDITKSEKGLKEIIQEIEKEILIDVLKNTGTTRKAAKKLKIDQSTLVKKLKKYGINID
HHEEHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHH
EIRHQS
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]