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The map label for this gene is proP [H]

Identifier: 138895918

GI number: 138895918

Start: 2395092

End: 2396576

Strand: Reverse

Name: proP [H]

Synonym: GTNG_2277

Alternate gene names: 138895918

Gene position: 2396576-2395092 (Counterclockwise)

Preceding gene: 138895919

Following gene: 138895917

Centisome position: 67.5

GC content: 40.94

Gene sequence:

>1485_bases
ATGAAGAATAGGACAAGGAATAGTGTAGACAGGTCCAACAGGATATTGAAAAAAGAAGATATTACCATTGTTGACACTAA
GCTTGCCAAAAAGGCGGTAGTTGCTACATCTTTAGGAAATGCAATGGAATGGTTTGATTTTGGCCTCTATTCTTACTTAG
CAGTGACCCTAGGAAAAGTATTCTTCCCTGAAACAGACCCTTCTTTGCAAATCATTTATTCATTCGCTACTTTTGCAGTC
GCATTTATTGTTCGCCCGATTGGCGGTATGTTTTTTGGAATGTTAGGGGACCGAATTGGTCGCAAAAGCGTTTTATCAAT
TACATTGATCATGATGGCGGCTGCAACCTTCAGTATCGGTTTAATTCCAAGTTACTCCTCTATAGGCATCACCGCTCCTA
TTTTATTGCTTGTTGCCCGGTTGGTCCAAGGATTTTCCACCGGGGGAGAATATGCAGGAGCTATGACATTCATCGCCGAA
TCAACCCCTGACAAAAAGCGCAGTTTTATGGCGAGCGGCCTAGAAGTTGGTACACTTGTCGGTTTTTCTGCTGGTGCTGG
ATTAGTGACATTGTTAACCTTCCTTCTTGGTCCTGAAAAAATGATTGAATGGGGATGGAGACTTCCATTTTTAATCGCTG
GTCCTCTAGGACTCATTGGTCTATACTTACGCTCTCGACTTGAAGAAACACCTGCTTTTCAGGCTATCATCAATGCGACG
GAAGAAGAAAAACAGCGTGCTTCACTTAAAGAAATTGTCATTTACCATTGGAAGCCGCTCTTAGTATGTATCGGAATTGT
TGTTTTTTACAATGTAGTCGACTATATGGTATTGACTTATATGCCATCGTATATGATGCAAGAACTTGGCTATGGTGAAA
TGAGAGGATTAGTTTTAACTTTAGTTTCTATGTTTATGATGATCCCTTTCATCCTACTTATGGGTTATTTGAGTGATCGA
GTTGGCCGTAACCGTATTGTCATCGGAGCGTTAATTGGAGGCATTGTCCTTAGTATACCTGCCTTTACGTTAATTAAGAG
CGGAAATAATTATACCGTTTTTTTAGGCTTGTTCCTGATCAGTATTTTGCTGACTGCTTTTCAAGGTGCGCTTCCGGCGC
TGCTCCCTTCCTTGTTTTTTACAAAGGTTCGTTATGGATCACTGGCTATCGCCTATAATGTATCCACATCGTTATTTGGG
GGGACTACACCACTCATTCTCGCTTGGTTAATTAGCAAGACGCAGAACGATATGATTCCAGCTTATTACTTAATGGGTGT
TTGTTTAGTTGGTTTGGTTATTGTTTCCTTGTTTGTAAAAGAAACAGCTGGAAAGTCGCTGCGTGGATCTGCTCCGGCTG
TCGAAGATCCAAGTGAGATCGGGGAAATATTAAAAAATCCGGATAAAGCTCTTTGGTGGAAAGAAGAGTTGACAGGCGAA
CAGATAGAGGATAATTATAAACCAAAAACATACGTAAATACGTAA

Upstream 100 bases:

>100_bases
ATCCAAGGAATTTTCCTTTCAGCAAAACGTTTTATGTTCAGGAGCCATTGCTCCTATCAGAAAGCTGACATAGAAACTGT
CATGGTAAAGGAGAGTTGTT

Downstream 100 bases:

>100_bases
ATATACATCGCTATGCCCGTGGTACTAATCAGACATGATTCAACTAGTACCACGGGTATTATTTGTACAAGGTTATTAAA
GTTGTATGAAAAAATATTTT

Product: proline-betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKNRTRNSVDRSNRILKKEDITIVDTKLAKKAVVATSLGNAMEWFDFGLYSYLAVTLGKVFFPETDPSLQIIYSFATFAV
AFIVRPIGGMFFGMLGDRIGRKSVLSITLIMMAAATFSIGLIPSYSSIGITAPILLLVARLVQGFSTGGEYAGAMTFIAE
STPDKKRSFMASGLEVGTLVGFSAGAGLVTLLTFLLGPEKMIEWGWRLPFLIAGPLGLIGLYLRSRLEETPAFQAIINAT
EEEKQRASLKEIVIYHWKPLLVCIGIVVFYNVVDYMVLTYMPSYMMQELGYGEMRGLVLTLVSMFMMIPFILLMGYLSDR
VGRNRIVIGALIGGIVLSIPAFTLIKSGNNYTVFLGLFLISILLTAFQGALPALLPSLFFTKVRYGSLAIAYNVSTSLFG
GTTPLILAWLISKTQNDMIPAYYLMGVCLVGLVIVSLFVKETAGKSLRGSAPAVEDPSEIGEILKNPDKALWWKEELTGE
QIEDNYKPKTYVNT

Sequences:

>Translated_494_residues
MKNRTRNSVDRSNRILKKEDITIVDTKLAKKAVVATSLGNAMEWFDFGLYSYLAVTLGKVFFPETDPSLQIIYSFATFAV
AFIVRPIGGMFFGMLGDRIGRKSVLSITLIMMAAATFSIGLIPSYSSIGITAPILLLVARLVQGFSTGGEYAGAMTFIAE
STPDKKRSFMASGLEVGTLVGFSAGAGLVTLLTFLLGPEKMIEWGWRLPFLIAGPLGLIGLYLRSRLEETPAFQAIINAT
EEEKQRASLKEIVIYHWKPLLVCIGIVVFYNVVDYMVLTYMPSYMMQELGYGEMRGLVLTLVSMFMMIPFILLMGYLSDR
VGRNRIVIGALIGGIVLSIPAFTLIKSGNNYTVFLGLFLISILLTAFQGALPALLPSLFFTKVRYGSLAIAYNVSTSLFG
GTTPLILAWLISKTQNDMIPAYYLMGVCLVGLVIVSLFVKETAGKSLRGSAPAVEDPSEIGEILKNPDKALWWKEELTGE
QIEDNYKPKTYVNT
>Mature_494_residues
MKNRTRNSVDRSNRILKKEDITIVDTKLAKKAVVATSLGNAMEWFDFGLYSYLAVTLGKVFFPETDPSLQIIYSFATFAV
AFIVRPIGGMFFGMLGDRIGRKSVLSITLIMMAAATFSIGLIPSYSSIGITAPILLLVARLVQGFSTGGEYAGAMTFIAE
STPDKKRSFMASGLEVGTLVGFSAGAGLVTLLTFLLGPEKMIEWGWRLPFLIAGPLGLIGLYLRSRLEETPAFQAIINAT
EEEKQRASLKEIVIYHWKPLLVCIGIVVFYNVVDYMVLTYMPSYMMQELGYGEMRGLVLTLVSMFMMIPFILLMGYLSDR
VGRNRIVIGALIGGIVLSIPAFTLIKSGNNYTVFLGLFLISILLTAFQGALPALLPSLFFTKVRYGSLAIAYNVSTSLFG
GTTPLILAWLISKTQNDMIPAYYLMGVCLVGLVIVSLFVKETAGKSLRGSAPAVEDPSEIGEILKNPDKALWWKEELTGE
QIEDNYKPKTYVNT

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=479, Percent_Identity=47.8079331941545, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=32.258064516129, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1789941, Length=443, Percent_Identity=35.2144469525959, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1788942, Length=402, Percent_Identity=29.3532338308458, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87082231, Length=483, Percent_Identity=24.8447204968944, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87082404, Length=415, Percent_Identity=26.7469879518072, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 54255; Mature: 54255

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRTRNSVDRSNRILKKEDITIVDTKLAKKAVVATSLGNAMEWFDFGLYSYLAVTLGKV
CCCCCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FFPETDPSLQIIYSFATFAVAFIVRPIGGMFFGMLGDRIGRKSVLSITLIMMAAATFSIG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
LIPSYSSIGITAPILLLVARLVQGFSTGGEYAGAMTFIAESTPDKKRSFMASGLEVGTLV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
GFSAGAGLVTLLTFLLGPEKMIEWGWRLPFLIAGPLGLIGLYLRSRLEETPAFQAIINAT
CCCCCCHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCH
EEEKQRASLKEIVIYHWKPLLVCIGIVVFYNVVDYMVLTYMPSYMMQELGYGEMRGLVLT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LVSMFMMIPFILLMGYLSDRVGRNRIVIGALIGGIVLSIPAFTLIKSGNNYTVFLGLFLI
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHH
SILLTAFQGALPALLPSLFFTKVRYGSLAIAYNVSTSLFGGTTPLILAWLISKTQNDMIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCH
AYYLMGVCLVGLVIVSLFVKETAGKSLRGSAPAVEDPSEIGEILKNPDKALWWKEELTGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHEEEHHHCCCC
QIEDNYKPKTYVNT
HHHCCCCCCCCCCC
>Mature Secondary Structure
MKNRTRNSVDRSNRILKKEDITIVDTKLAKKAVVATSLGNAMEWFDFGLYSYLAVTLGKV
CCCCCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FFPETDPSLQIIYSFATFAVAFIVRPIGGMFFGMLGDRIGRKSVLSITLIMMAAATFSIG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
LIPSYSSIGITAPILLLVARLVQGFSTGGEYAGAMTFIAESTPDKKRSFMASGLEVGTLV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
GFSAGAGLVTLLTFLLGPEKMIEWGWRLPFLIAGPLGLIGLYLRSRLEETPAFQAIINAT
CCCCCCHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCH
EEEKQRASLKEIVIYHWKPLLVCIGIVVFYNVVDYMVLTYMPSYMMQELGYGEMRGLVLT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LVSMFMMIPFILLMGYLSDRVGRNRIVIGALIGGIVLSIPAFTLIKSGNNYTVFLGLFLI
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHH
SILLTAFQGALPALLPSLFFTKVRYGSLAIAYNVSTSLFGGTTPLILAWLISKTQNDMIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCH
AYYLMGVCLVGLVIVSLFVKETAGKSLRGSAPAVEDPSEIGEILKNPDKALWWKEELTGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHEEEHHHCCCC
QIEDNYKPKTYVNT
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]