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The map label for this gene is nagE [H]

Identifier: 138895843

GI number: 138895843

Start: 2325962

End: 2327332

Strand: Reverse

Name: nagE [H]

Synonym: GTNG_2202

Alternate gene names: 138895843

Gene position: 2327332-2325962 (Counterclockwise)

Preceding gene: 138895844

Following gene: 138895842

Centisome position: 65.55

GC content: 48.07

Gene sequence:

>1371_bases
ATGTTTATGTTAGGATTTTTACAGCGTCTAGGAAAGGCGTTTATGCTGCCGATTGCCGTTTTGCCGGCGGCTGGACTGTT
GCTCCGGCTTGGTCAGGACGACTTATTAGATATTCCATTTATCGCTGCTGCGGGTGATGCGGTGTTTTCCAACTTGGCGT
TGATTTTCGCCATTGGTGTCGCCATCGGCTTTTCAAAAGATGGCAATGGTGCTGCCGCGCTCGCTGGGGCGATCGGCTAT
TTTGTTTTGACGAAAGGCGCTGCCGCGATTGATAAAGACATCAATATGTCCGTATTAGGCGGTATCATCTCCGGGGTGAT
CGCCGGGCTTTTATACAATCGTTATCATGATATCAAGTTGCCGGACTGGCTCGGCTTTTTCGGTGGCCGGCGGTTTGTGC
CGATCGTAACATCGCTTGTTATGATCGTGCTTGCTCTCGTTTTCGGATACATTTGGCCGCCGATTCAAGACGGCATTAAT
GCGATCGGTCATTGGATCGTGGGCGCCGGGGCCGTTGGCGTTGGGATTTTTGGGTTCTTAAACCGGTTGCTCATTCCTGT
TGGGCTGCACCATGTGTTAAACAGTTTCGTCTGGTTTGTGTTTGGTGAGTATGACGGTAAAACCGGTGATTTAAGCCGAT
TTTTTGCCGGTGACCCGGATGCAGGGATTTTTATGGCTGGCTTCTTCCCGGTCATGATGTTCGGTCTCCCGGCGGCGGCA
TTGGCGATGATCATCGCGGCGAAGAAGGAACGGCGCAAAGCGATTGCCGGTGCGCTCCTTGGCGTAGCGTTGACATCGTT
TTTAACCGGTATTACAGAGCCGATCGAATTTTTGTTTATGTTTTTATCGCCGCTCTTGTATGTCGTTCATGCCGTGTTAA
CTGGCTTATCGCTGGCGGTGGCGACGGCATTTGATATTCATCACGGGTTTACGTTCTCGGCCGGTGCGATTGACTTTTTC
TTAAACTATGGAATTGCACAAAAACCGTATTTATTAATTGTTCAAGGACTCATCTATGCTGTGATCTATTTCGTTGTTTT
CTATTTCCTGATTACGAAGCTCAATTTAAAAACGCCGGGACGTGAAGATGACGTAGAGGGAGAGTTTACCGACAGCAAAG
CGGCGCAGTCGGGCGTAAAATATGAAGAGCTAGCGGTAAAATATATAGAGGCATTGGGAGGAAAAGAAAACCTCACACAA
ATTGATAATTGTGTCACACGCTTGCGCTTAAAAGTAAAAGACATGTCAAAAGTCAACGAAGCTGAATTGAAGCGCTTAGG
GGCGAAAGGGGTGCTTCGTCTCAACCAAACGGATTTGCAAGTGATCATTGGCACGGATGTCGAATTTTTAGCCAATGCGA
TGAGAAAATAA

Upstream 100 bases:

>100_bases
CCTTACTGACGGACGGCCACTGGAAGTTGTGAAATCAGTGTATCGCGGTGACCGATACAAATTCATCGTTGAAATGGAAC
GAAGAAAATAAAAAGGGGGA

Downstream 100 bases:

>100_bases
TCAAAAGTGAAATGGACAGATCGTTCTTGTATGCGAACGTTAGGAAGGAAGAGCGCTTATGAAACGATTGTTAGATTGCA
GCGCCTCCGATTTCCGCAAG

Product: PTS system N-acetylglucosamine-specific transporter subunit IIABC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MFMLGFLQRLGKAFMLPIAVLPAAGLLLRLGQDDLLDIPFIAAAGDAVFSNLALIFAIGVAIGFSKDGNGAAALAGAIGY
FVLTKGAAAIDKDINMSVLGGIISGVIAGLLYNRYHDIKLPDWLGFFGGRRFVPIVTSLVMIVLALVFGYIWPPIQDGIN
AIGHWIVGAGAVGVGIFGFLNRLLIPVGLHHVLNSFVWFVFGEYDGKTGDLSRFFAGDPDAGIFMAGFFPVMMFGLPAAA
LAMIIAAKKERRKAIAGALLGVALTSFLTGITEPIEFLFMFLSPLLYVVHAVLTGLSLAVATAFDIHHGFTFSAGAIDFF
LNYGIAQKPYLLIVQGLIYAVIYFVVFYFLITKLNLKTPGREDDVEGEFTDSKAAQSGVKYEELAVKYIEALGGKENLTQ
IDNCVTRLRLKVKDMSKVNEAELKRLGAKGVLRLNQTDLQVIIGTDVEFLANAMRK

Sequences:

>Translated_456_residues
MFMLGFLQRLGKAFMLPIAVLPAAGLLLRLGQDDLLDIPFIAAAGDAVFSNLALIFAIGVAIGFSKDGNGAAALAGAIGY
FVLTKGAAAIDKDINMSVLGGIISGVIAGLLYNRYHDIKLPDWLGFFGGRRFVPIVTSLVMIVLALVFGYIWPPIQDGIN
AIGHWIVGAGAVGVGIFGFLNRLLIPVGLHHVLNSFVWFVFGEYDGKTGDLSRFFAGDPDAGIFMAGFFPVMMFGLPAAA
LAMIIAAKKERRKAIAGALLGVALTSFLTGITEPIEFLFMFLSPLLYVVHAVLTGLSLAVATAFDIHHGFTFSAGAIDFF
LNYGIAQKPYLLIVQGLIYAVIYFVVFYFLITKLNLKTPGREDDVEGEFTDSKAAQSGVKYEELAVKYIEALGGKENLTQ
IDNCVTRLRLKVKDMSKVNEAELKRLGAKGVLRLNQTDLQVIIGTDVEFLANAMRK
>Mature_456_residues
MFMLGFLQRLGKAFMLPIAVLPAAGLLLRLGQDDLLDIPFIAAAGDAVFSNLALIFAIGVAIGFSKDGNGAAALAGAIGY
FVLTKGAAAIDKDINMSVLGGIISGVIAGLLYNRYHDIKLPDWLGFFGGRRFVPIVTSLVMIVLALVFGYIWPPIQDGIN
AIGHWIVGAGAVGVGIFGFLNRLLIPVGLHHVLNSFVWFVFGEYDGKTGDLSRFFAGDPDAGIFMAGFFPVMMFGLPAAA
LAMIIAAKKERRKAIAGALLGVALTSFLTGITEPIEFLFMFLSPLLYVVHAVLTGLSLAVATAFDIHHGFTFSAGAIDFF
LNYGIAQKPYLLIVQGLIYAVIYFVVFYFLITKLNLKTPGREDDVEGEFTDSKAAQSGVKYEELAVKYIEALGGKENLTQ
IDNCVTRLRLKVKDMSKVNEAELKRLGAKGVLRLNQTDLQVIIGTDVEFLANAMRK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=464, Percent_Identity=58.8362068965517, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1787343, Length=475, Percent_Identity=43.1578947368421, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1787908, Length=511, Percent_Identity=38.5518590998043, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49177; Mature: 49177

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMLGFLQRLGKAFMLPIAVLPAAGLLLRLGQDDLLDIPFIAAAGDAVFSNLALIFAIGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHH
AIGFSKDGNGAAALAGAIGYFVLTKGAAAIDKDINMSVLGGIISGVIAGLLYNRYHDIKL
HHCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PDWLGFFGGRRFVPIVTSLVMIVLALVFGYIWPPIQDGINAIGHWIVGAGAVGVGIFGFL
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
NRLLIPVGLHHVLNSFVWFVFGEYDGKTGDLSRFFAGDPDAGIFMAGFFPVMMFGLPAAA
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCHHH
LAMIIAAKKERRKAIAGALLGVALTSFLTGITEPIEFLFMFLSPLLYVVHAVLTGLSLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATAFDIHHGFTFSAGAIDFFLNYGIAQKPYLLIVQGLIYAVIYFVVFYFLITKLNLKTPG
HHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
REDDVEGEFTDSKAAQSGVKYEELAVKYIEALGGKENLTQIDNCVTRLRLKVKDMSKVNE
CCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCH
AELKRLGAKGVLRLNQTDLQVIIGTDVEFLANAMRK
HHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHCC
>Mature Secondary Structure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HHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]