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The map label for this gene is gutB [H]

Identifier: 138895597

GI number: 138895597

Start: 2073801

End: 2074799

Strand: Reverse

Name: gutB [H]

Synonym: GTNG_1950

Alternate gene names: 138895597

Gene position: 2074799-2073801 (Counterclockwise)

Preceding gene: 138895598

Following gene: 138895596

Centisome position: 58.44

GC content: 52.25

Gene sequence:

>999_bases
ATGTTAGCGCTTTATGTAACAAAGCCGAATGAGCTGGAATTGCGGCATCTTGATGGCATCCGTTCTCCCGTGAGTGATGA
GGTGAAAATCAAGCTGATTTACGGGGGGATTTGCGGATCGGACCTCGGGGTGTTCAAAGGAAAACTCCCGCATGCGAACT
ATCCGGTCCGGGCGGGCCATGAGCTTGTCGGCGTCGTCGTGGAAAAAGGTGAACAGGCGGCGTATGACATTGGGACGCGG
GTCGTTGTGCTGCCGAATACGTATTGCGGAACGTGCGACTTATGCCAAAAAGGTTATACAAACATATGTCGGCATAAACA
ATCACTCGGCATTAACGTCGACGGTGGGTTTTCGCATGAGTTTGTCATCACATCGAAGTACGTACTTTCTATTCCGGATG
ATTTACCAGACGAAAAGGCCGTGCTTGTCGAGCCGTTTGCGGTTGTCGTGCATGCGTTGCAAAAGGTGAAAATCAAACAG
GGGATGAAAGTAGCCATCATCGGCTGCGGCAATGAAGGGATGATGGCAGCGGTGTTAGCCCGCTATTTGGGGGCGGACGT
GACGGCGAGCGACATCAATCCACTGAAACTCGAAACGGTGAAAACGATGGCGGACATTCGCACGCTTGCGCCGAGTGCAC
TGGGCAATGAATCGTTTGACGTGGTCATTGAAGCAGCGGGTACGCGCGAAGCGGTCGAACAGGCAGTGGAGCTCGTCGCC
CCAGGCGGTCATCTTGTGTTGATCGGTTTTGCGAATGAAGCGACGTTTCCGGTCGTGCGCCTCGTTCGCAACGAAGTAAC
TGTGCATGGTTCGGTCATCTATCGCTTCCCGGATGACTATTTGCAAGCGATTCATTATTTGCGGACGATGCCGTATCCGA
TCGAGCGGGTGATTTCGTGCATTTTCCCAGTTCGTGACTATCAGCAGGCGTATGAGTTGGCGTCATCCGGAAACATGTGC
AAAGTTGTTTTAAGCTTTAAAGAGGAGGAAGAGCGATGA

Upstream 100 bases:

>100_bases
CGGCATCGGCCGCGAAGGTCCTCGTTATGCGATCGAAGAAATGACGGAAATGAAAATGATTGTATGGCAGTTTCCACAGT
GATGATGGGTGGTGGACAAC

Downstream 100 bases:

>100_bases
AAAAAGGAGTAGCCGAACAGTTAGTCCGGTATTTGGAAGACCGCGGTGTCACGCATATCTTCGGGCTTTGCGGGCATACG
AATATCGCGGTGTTGGCAGC

Product: sorbitol dehydrogenase (L-iditol 2-dehydrogenase)

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLALYVTKPNELELRHLDGIRSPVSDEVKIKLIYGGICGSDLGVFKGKLPHANYPVRAGHELVGVVVEKGEQAAYDIGTR
VVVLPNTYCGTCDLCQKGYTNICRHKQSLGINVDGGFSHEFVITSKYVLSIPDDLPDEKAVLVEPFAVVVHALQKVKIKQ
GMKVAIIGCGNEGMMAAVLARYLGADVTASDINPLKLETVKTMADIRTLAPSALGNESFDVVIEAAGTREAVEQAVELVA
PGGHLVLIGFANEATFPVVRLVRNEVTVHGSVIYRFPDDYLQAIHYLRTMPYPIERVISCIFPVRDYQQAYELASSGNMC
KVVLSFKEEEER

Sequences:

>Translated_332_residues
MLALYVTKPNELELRHLDGIRSPVSDEVKIKLIYGGICGSDLGVFKGKLPHANYPVRAGHELVGVVVEKGEQAAYDIGTR
VVVLPNTYCGTCDLCQKGYTNICRHKQSLGINVDGGFSHEFVITSKYVLSIPDDLPDEKAVLVEPFAVVVHALQKVKIKQ
GMKVAIIGCGNEGMMAAVLARYLGADVTASDINPLKLETVKTMADIRTLAPSALGNESFDVVIEAAGTREAVEQAVELVA
PGGHLVLIGFANEATFPVVRLVRNEVTVHGSVIYRFPDDYLQAIHYLRTMPYPIERVISCIFPVRDYQQAYELASSGNMC
KVVLSFKEEEER
>Mature_332_residues
MLALYVTKPNELELRHLDGIRSPVSDEVKIKLIYGGICGSDLGVFKGKLPHANYPVRAGHELVGVVVEKGEQAAYDIGTR
VVVLPNTYCGTCDLCQKGYTNICRHKQSLGINVDGGFSHEFVITSKYVLSIPDDLPDEKAVLVEPFAVVVHALQKVKIKQ
GMKVAIIGCGNEGMMAAVLARYLGADVTASDINPLKLETVKTMADIRTLAPSALGNESFDVVIEAAGTREAVEQAVELVA
PGGHLVLIGFANEATFPVVRLVRNEVTVHGSVIYRFPDDYLQAIHYLRTMPYPIERVISCIFPVRDYQQAYELASSGNMC
KVVLSFKEEEER

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=347, Percent_Identity=24.7838616714697, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI71743840, Length=345, Percent_Identity=24.9275362318841, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI262073058, Length=345, Percent_Identity=24.9275362318841, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI226510992, Length=343, Percent_Identity=30.0291545189504, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787863, Length=344, Percent_Identity=29.0697674418605, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788407, Length=268, Percent_Identity=33.2089552238806, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788073, Length=308, Percent_Identity=29.5454545454545, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790045, Length=317, Percent_Identity=26.1829652996845, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790718, Length=307, Percent_Identity=27.0358306188925, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788075, Length=337, Percent_Identity=23.7388724035608, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082125, Length=352, Percent_Identity=25.5681818181818, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87081918, Length=288, Percent_Identity=29.1666666666667, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082401, Length=334, Percent_Identity=27.2455089820359, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786825, Length=224, Percent_Identity=27.6785714285714, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786518, Length=282, Percent_Identity=28.0141843971631, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=326, Percent_Identity=26.3803680981595, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17562878, Length=340, Percent_Identity=25.2941176470588, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71988145, Length=269, Percent_Identity=28.996282527881, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562584, Length=283, Percent_Identity=27.9151943462898, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=291, Percent_Identity=28.1786941580756, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25146526, Length=361, Percent_Identity=23.2686980609418, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71997431, Length=363, Percent_Identity=23.1404958677686, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319955, Length=339, Percent_Identity=27.1386430678466, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322619, Length=339, Percent_Identity=27.1386430678466, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323099, Length=336, Percent_Identity=26.7857142857143, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6319258, Length=226, Percent_Identity=28.7610619469027, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319257, Length=324, Percent_Identity=26.2345679012346, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320033, Length=356, Percent_Identity=24.1573033707865, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=286, Percent_Identity=26.2237762237762, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=263, Percent_Identity=26.615969581749, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=277, Percent_Identity=24.9097472924188, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324486, Length=191, Percent_Identity=28.7958115183246, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17137530, Length=313, Percent_Identity=24.9201277955272, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17737897, Length=313, Percent_Identity=23.3226837060703, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17737895, Length=348, Percent_Identity=23.8505747126437, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36321; Mature: 36321

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALYVTKPNELELRHLDGIRSPVSDEVKIKLIYGGICGSDLGVFKGKLPHANYPVRAGH
CEEEEEECCCCEEEHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCC
ELVGVVVEKGEQAAYDIGTRVVVLPNTYCGTCDLCQKGYTNICRHKQSLGINVDGGFSHE
CEEEEEEECCCCHHEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCEE
FVITSKYVLSIPDDLPDEKAVLVEPFAVVVHALQKVKIKQGMKVAIIGCGNEGMMAAVLA
EEEEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
RYLGADVTASDINPLKLETVKTMADIRTLAPSALGNESFDVVIEAAGTREAVEQAVELVA
HHHCCCCCCCCCCCEEHHHHHHHHHHHHHCHHHCCCCCEEEEEEECCCHHHHHHHHHHCC
PGGHLVLIGFANEATFPVVRLVRNEVTVHGSVIYRFPDDYLQAIHYLRTMPYPIERVISC
CCCCEEEEEECCCCHHHHHHHHHHCEEEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHH
IFPVRDYQQAYELASSGNMCKVVLSFKEEEER
HHCHHHHHHHHHHHCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MLALYVTKPNELELRHLDGIRSPVSDEVKIKLIYGGICGSDLGVFKGKLPHANYPVRAGH
CEEEEEECCCCEEEHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCC
ELVGVVVEKGEQAAYDIGTRVVVLPNTYCGTCDLCQKGYTNICRHKQSLGINVDGGFSHE
CEEEEEEECCCCHHEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCEE
FVITSKYVLSIPDDLPDEKAVLVEPFAVVVHALQKVKIKQGMKVAIIGCGNEGMMAAVLA
EEEEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
RYLGADVTASDINPLKLETVKTMADIRTLAPSALGNESFDVVIEAAGTREAVEQAVELVA
HHHCCCCCCCCCCCEEHHHHHHHHHHHHHCHHHCCCCCEEEEEEECCCHHHHHHHHHHCC
PGGHLVLIGFANEATFPVVRLVRNEVTVHGSVIYRFPDDYLQAIHYLRTMPYPIERVISC
CCCCEEEEEECCCCHHHHHHHHHHCEEEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHH
IFPVRDYQQAYELASSGNMCKVVLSFKEEEER
HHCHHHHHHHHHHHCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]