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The map label for this gene is ilvB [H]

Identifier: 138895596

GI number: 138895596

Start: 2072041

End: 2073804

Strand: Reverse

Name: ilvB [H]

Synonym: GTNG_1949

Alternate gene names: 138895596

Gene position: 2073804-2072041 (Counterclockwise)

Preceding gene: 138895597

Following gene: 138895595

Centisome position: 58.41

GC content: 52.15

Gene sequence:

>1764_bases
ATGAAAAAAGGAGTAGCCGAACAGTTAGTCCGGTATTTGGAAGACCGCGGTGTCACGCATATCTTCGGGCTTTGCGGGCA
TACGAATATCGCGGTGTTGGCAGCGCTCGAGCATAGCCCGATCAAGTTTGTCAACGTCCGCCATGAACAAATTGCTGCCC
ATATGGCGGATGGATATGCGCGGGCGAAAAAAGAAACAGCCGTCGTATTATGCCACGTCGGCCCGGGGTTGACGAATGCG
GCGACAGGGGTCGCCAATGCGGCGCTTGACTCCATTCCGATGGTCGTTATTGCCGGCGATGTGCCGAGCCATTATTATGG
GAAACATCCGCACCAAGAAATCAACTTGCATGCGGATGCGTCACAATATGAAATTTACCGCCCGTTTGTGAAGCGGGCAT
GGCGGGTCGACCGGCCGGATTTGTTCCCGGAAATTTTAGAAAAAGCGTTCCAGCTGGCGGAGAGCGGTCGTCCGGGTCCG
GTGCTCGTCTCCGTACCGATGGATATTTTCTCAAAAGAGGTGGACGTGTCGCTGTTTGAAAAACAAAAGCGGCATACGAA
AGCGTTGCATAAGCCGTCGATTGATGATGAAACAGCAAAACAAATCATTGAAAACATCATGCAGGCAGAGCGTCCGCTTG
TGTATGTTGGCGGTGGCATTATGCTAGCCGATGCCGCGAAAGAACTGAAAGCGTTTGTCGAACACTTTGACCTTCCTGTT
GCTCACACGTTAATGGGGAAAGGAGTGATGCCGGATGACCATCCGCTGACGCTGGGCATGAGCGGATTTTGGGGAACGAA
ATTTGTCAATGACATGTGCCGCAACGCTGATGTCATCTTCGCGTTAGGAACGAGATTTTCCGAAGCAGATTGCAGCTCGT
GGGAGTCGGAATATACGTTTGCCATACCGGAAACAAAGCTGATTCATATTGATATTGATCCGAACGAAATCGGACGCAAC
TATCCAACGGAACTCGGCATTGTCGCCGATGTCAAACAAGCGCTTACCGTGTTGAACCGCGTGGCGAAACAAGTTGTTCC
GCAAGGACGGACGGACGAGGCGCTGAAAGAACGGATCACCAACTATAAGCAAGAATTCTGGAAACAAAACGCACAGCATA
TTTGCGATCATCGGTATCCGATGCGTCCGGAACGCATTTTGCATGAAGTGCGGGAAGTATTGCCAAGAAACGCCATCATT
ACAACGGATGTCGGTTGGAACAAAAACGGGGTCGGTCAACAGTTCCCGGCGTATGGAGCAGGGACGATTTTAACGCCAGG
TGGCTATGCGACGATGGGCTTTGGCGCTCCGGCGGCGCTCGGGGCGAAAATCGCCTGCCCGGATCGCGTCGTCGTGTCGC
TTGTTGGAGATGGCGGATTTGGGCAAAATCCGGCGGTGCTGGCGACAGCGGCTGAGGAAAATATCGCCGTTGTTTGGGTC
ATTATGAACAACTACGCTTTTGGCACGATCGCCGGACTGGAAAAAGCACATTTTGGCACGACACACGGAACAGTCTTCCA
AAAAGATGGTCAACCGTATTCGCCGGATTATGCGGCCATTGCCCAGGCGTACGGCATCGAAGGAGTGAAAATCCGCTCAG
CGGAAGAGTTTAAACCAACGCTCGAGCGAGCCATCCAAGCGAACAAACCGTTTGTCATCGATGTGGAAATGATCAACGTC
CCAACGCCGACATCTGGTCATTGGAATATTATGGATATCTATTCCCCGGGTAAGCGTGTTCACCATGTTGCGGTTGAGCG
GTAA

Upstream 100 bases:

>100_bases
CGTGCATTTTCCCAGTTCGTGACTATCAGCAGGCGTATGAGTTGGCGTCATCCGGAAACATGTGCAAAGTTGTTTTAAGC
TTTAAAGAGGAGGAAGAGCG

Downstream 100 bases:

>100_bases
TGAGGAACATTGAAGATTGGGGGGGACGGATATGGCGAGAACCTTTTCATTGGCTCATTTGACGGTACTCGGCTGCCCGC
CGCCAGAAATGACGCACATC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKKGVAEQLVRYLEDRGVTHIFGLCGHTNIAVLAALEHSPIKFVNVRHEQIAAHMADGYARAKKETAVVLCHVGPGLTNA
ATGVANAALDSIPMVVIAGDVPSHYYGKHPHQEINLHADASQYEIYRPFVKRAWRVDRPDLFPEILEKAFQLAESGRPGP
VLVSVPMDIFSKEVDVSLFEKQKRHTKALHKPSIDDETAKQIIENIMQAERPLVYVGGGIMLADAAKELKAFVEHFDLPV
AHTLMGKGVMPDDHPLTLGMSGFWGTKFVNDMCRNADVIFALGTRFSEADCSSWESEYTFAIPETKLIHIDIDPNEIGRN
YPTELGIVADVKQALTVLNRVAKQVVPQGRTDEALKERITNYKQEFWKQNAQHICDHRYPMRPERILHEVREVLPRNAII
TTDVGWNKNGVGQQFPAYGAGTILTPGGYATMGFGAPAALGAKIACPDRVVVSLVGDGGFGQNPAVLATAAEENIAVVWV
IMNNYAFGTIAGLEKAHFGTTHGTVFQKDGQPYSPDYAAIAQAYGIEGVKIRSAEEFKPTLERAIQANKPFVIDVEMINV
PTPTSGHWNIMDIYSPGKRVHHVAVER

Sequences:

>Translated_587_residues
MKKGVAEQLVRYLEDRGVTHIFGLCGHTNIAVLAALEHSPIKFVNVRHEQIAAHMADGYARAKKETAVVLCHVGPGLTNA
ATGVANAALDSIPMVVIAGDVPSHYYGKHPHQEINLHADASQYEIYRPFVKRAWRVDRPDLFPEILEKAFQLAESGRPGP
VLVSVPMDIFSKEVDVSLFEKQKRHTKALHKPSIDDETAKQIIENIMQAERPLVYVGGGIMLADAAKELKAFVEHFDLPV
AHTLMGKGVMPDDHPLTLGMSGFWGTKFVNDMCRNADVIFALGTRFSEADCSSWESEYTFAIPETKLIHIDIDPNEIGRN
YPTELGIVADVKQALTVLNRVAKQVVPQGRTDEALKERITNYKQEFWKQNAQHICDHRYPMRPERILHEVREVLPRNAII
TTDVGWNKNGVGQQFPAYGAGTILTPGGYATMGFGAPAALGAKIACPDRVVVSLVGDGGFGQNPAVLATAAEENIAVVWV
IMNNYAFGTIAGLEKAHFGTTHGTVFQKDGQPYSPDYAAIAQAYGIEGVKIRSAEEFKPTLERAIQANKPFVIDVEMINV
PTPTSGHWNIMDIYSPGKRVHHVAVER
>Mature_587_residues
MKKGVAEQLVRYLEDRGVTHIFGLCGHTNIAVLAALEHSPIKFVNVRHEQIAAHMADGYARAKKETAVVLCHVGPGLTNA
ATGVANAALDSIPMVVIAGDVPSHYYGKHPHQEINLHADASQYEIYRPFVKRAWRVDRPDLFPEILEKAFQLAESGRPGP
VLVSVPMDIFSKEVDVSLFEKQKRHTKALHKPSIDDETAKQIIENIMQAERPLVYVGGGIMLADAAKELKAFVEHFDLPV
AHTLMGKGVMPDDHPLTLGMSGFWGTKFVNDMCRNADVIFALGTRFSEADCSSWESEYTFAIPETKLIHIDIDPNEIGRN
YPTELGIVADVKQALTVLNRVAKQVVPQGRTDEALKERITNYKQEFWKQNAQHICDHRYPMRPERILHEVREVLPRNAII
TTDVGWNKNGVGQQFPAYGAGTILTPGGYATMGFGAPAALGAKIACPDRVVVSLVGDGGFGQNPAVLATAAEENIAVVWV
IMNNYAFGTIAGLEKAHFGTTHGTVFQKDGQPYSPDYAAIAQAYGIEGVKIRSAEEFKPTLERAIQANKPFVIDVEMINV
PTPTSGHWNIMDIYSPGKRVHHVAVER

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=26.0105448154657, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI21361361, Length=572, Percent_Identity=26.9230769230769, Blast_Score=147, Evalue=4e-35,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=34.6358792184725, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=32.258064516129, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1786717, Length=574, Percent_Identity=31.5331010452962, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1787096, Length=562, Percent_Identity=26.3345195729537, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788716, Length=572, Percent_Identity=24.8251748251748, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=581, Percent_Identity=28.9156626506024, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=580, Percent_Identity=28.7931034482759, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=529, Percent_Identity=26.0869565217391, Blast_Score=145, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=30.2654867256637, Blast_Score=233, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6320816, Length=567, Percent_Identity=23.9858906525573, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321524, Length=503, Percent_Identity=22.0675944333996, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922626, Length=575, Percent_Identity=27.304347826087, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64616; Mature: 64616

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00187 TPP_ENZYMES ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGVAEQLVRYLEDRGVTHIFGLCGHTNIAVLAALEHSPIKFVNVRHEQIAAHMADGYA
CCCCHHHHHHHHHHHCCCEEEEHHCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHH
RAKKETAVVLCHVGPGLTNAATGVANAALDSIPMVVIAGDVPSHYYGKHPHQEINLHADA
HCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCC
SQYEIYRPFVKRAWRVDRPDLFPEILEKAFQLAESGRPGPVLVSVPMDIFSKEVDVSLFE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCHHHHH
KQKRHTKALHKPSIDDETAKQIIENIMQAERPLVYVGGGIMLADAAKELKAFVEHFDLPV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHHCCCH
AHTLMGKGVMPDDHPLTLGMSGFWGTKFVNDMCRNADVIFALGTRFSEADCSSWESEYTF
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEE
AIPETKLIHIDIDPNEIGRNYPTELGIVADVKQALTVLNRVAKQVVPQGRTDEALKERIT
ECCCEEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
NYKQEFWKQNAQHICDHRYPMRPERILHEVREVLPRNAIITTDVGWNKNGVGQQFPAYGA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
GTILTPGGYATMGFGAPAALGAKIACPDRVVVSLVGDGGFGQNPAVLATAAEENIAVVWV
CCEECCCCCEEECCCCHHHCCCEECCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEEE
IMNNYAFGTIAGLEKAHFGTTHGTVFQKDGQPYSPDYAAIAQAYGIEGVKIRSAEEFKPT
EECCCCEEEHHCCHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHH
LERAIQANKPFVIDVEMINVPTPTSGHWNIMDIYSPGKRVHHVAVER
HHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MKKGVAEQLVRYLEDRGVTHIFGLCGHTNIAVLAALEHSPIKFVNVRHEQIAAHMADGYA
CCCCHHHHHHHHHHHCCCEEEEHHCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHH
RAKKETAVVLCHVGPGLTNAATGVANAALDSIPMVVIAGDVPSHYYGKHPHQEINLHADA
HCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCC
SQYEIYRPFVKRAWRVDRPDLFPEILEKAFQLAESGRPGPVLVSVPMDIFSKEVDVSLFE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCHHHHH
KQKRHTKALHKPSIDDETAKQIIENIMQAERPLVYVGGGIMLADAAKELKAFVEHFDLPV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHHCCCH
AHTLMGKGVMPDDHPLTLGMSGFWGTKFVNDMCRNADVIFALGTRFSEADCSSWESEYTF
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEE
AIPETKLIHIDIDPNEIGRNYPTELGIVADVKQALTVLNRVAKQVVPQGRTDEALKERIT
ECCCEEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
NYKQEFWKQNAQHICDHRYPMRPERILHEVREVLPRNAIITTDVGWNKNGVGQQFPAYGA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
GTILTPGGYATMGFGAPAALGAKIACPDRVVVSLVGDGGFGQNPAVLATAAEENIAVVWV
CCEECCCCCEEECCCCHHHCCCEECCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEEE
IMNNYAFGTIAGLEKAHFGTTHGTVFQKDGQPYSPDYAAIAQAYGIEGVKIRSAEEFKPT
EECCCCEEEHHCCHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHH
LERAIQANKPFVIDVEMINVPTPTSGHWNIMDIYSPGKRVHHVAVER
HHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]