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The map label for this gene is yxjB [H]

Identifier: 138895233

GI number: 138895233

Start: 1659520

End: 1660395

Strand: Direct

Name: yxjB [H]

Synonym: GTNG_1573

Alternate gene names: 138895233

Gene position: 1659520-1660395 (Clockwise)

Preceding gene: 138895232

Following gene: 138895236

Centisome position: 46.74

GC content: 40.07

Gene sequence:

>876_bases
ATGACCAAAAAAACCAAAAGTGCAGAACGGGTTAGTCAATTTGTAGCGGCATTTCAATGTCCACTCTGTAAAAGCGCGAT
GAGGGTTGAGGAGCTGAAAAGCCTCGTATGCGCTAAAAACCATACGTTTGATTTCTCAAAACAAGGATATGTCAACTTGA
TGACTCGTCCCGCTCATAGCCATTACAAGAAGGAATTGTTTGAAGCAAGACACAAAATCATTATGGAAAGCAACGTATAC
ACTTCCATGCATGAAACGATTGCGAAAGTAATAAAGGAGTATATGGATGTTTCGCTTAACCCTTTTATGGTTATAGATTT
AGGATGCGGAGAAGGTTCTCACTTACAAAGGATTTTGGACGAATGTATGATTCCGTCAATGACTGGCGTTGGCCTCGATA
TTTCAAAAGAAGGCATCGCTATGGCTGCTAAAAGATACGAAGACCAAATTTGGCTAGTCGGTGACTTGGCAAAATCGCCG
CTGGCGGATCAATCGTTTCATGTGATTCTTAATATTTTATCTCCTTCCAATTATAAGGAATTCAAGAGGATTTTAGTTAA
AGATGGACTCGTGATTAAAGTGGTTCCTCGCCCGAATTATCTAAGAGAGCTACGAGAAGCACTCTTTGATGACAAGGAGA
AAAAGGTCTATAAGAATGATGAAATCGTGTCGCTTTTTCAAAAACAGCTCCATTTACTGGATGTATTCCATTTATGTTAT
ACAAAAGAACTTAATAATGCCGAACTTGAACATTTAGTCCAAATGACGCCGCTTGCTTGGTCTGCGGATCAAGCACACAT
TGATGCGTTTCTTCATCGGGATTTTGCGAAGATCACCGTGGATTTGGATATTTTAGTTGGAGTGAACAGGGGATGA

Upstream 100 bases:

>100_bases
AGAAATCATAACAGCAAATGGCGCAACTGGTTAAGCCAAAAGACTGTCACGAACGTTTCCTCCTCCGCTGTGATTTTTTA
TATCGAAAGGAAGAAAGCAC

Downstream 100 bases:

>100_bases
AGCAGCATGTCTAAGATGGAGTGCGCGAACAAGCAATGTGTTGATCAATATCAGCACATTGCTTGTTCTTTTTTCGACAG
AACTTACTGGTTACTATTTT

Product: ribosomal RNA large subunit methyltransferase A

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTKKTKSAERVSQFVAAFQCPLCKSAMRVEELKSLVCAKNHTFDFSKQGYVNLMTRPAHSHYKKELFEARHKIIMESNVY
TSMHETIAKVIKEYMDVSLNPFMVIDLGCGEGSHLQRILDECMIPSMTGVGLDISKEGIAMAAKRYEDQIWLVGDLAKSP
LADQSFHVILNILSPSNYKEFKRILVKDGLVIKVVPRPNYLRELREALFDDKEKKVYKNDEIVSLFQKQLHLLDVFHLCY
TKELNNAELEHLVQMTPLAWSADQAHIDAFLHRDFAKITVDLDILVGVNRG

Sequences:

>Translated_291_residues
MTKKTKSAERVSQFVAAFQCPLCKSAMRVEELKSLVCAKNHTFDFSKQGYVNLMTRPAHSHYKKELFEARHKIIMESNVY
TSMHETIAKVIKEYMDVSLNPFMVIDLGCGEGSHLQRILDECMIPSMTGVGLDISKEGIAMAAKRYEDQIWLVGDLAKSP
LADQSFHVILNILSPSNYKEFKRILVKDGLVIKVVPRPNYLRELREALFDDKEKKVYKNDEIVSLFQKQLHLLDVFHLCY
TKELNNAELEHLVQMTPLAWSADQAHIDAFLHRDFAKITVDLDILVGVNRG
>Mature_290_residues
TKKTKSAERVSQFVAAFQCPLCKSAMRVEELKSLVCAKNHTFDFSKQGYVNLMTRPAHSHYKKELFEARHKIIMESNVYT
SMHETIAKVIKEYMDVSLNPFMVIDLGCGEGSHLQRILDECMIPSMTGVGLDISKEGIAMAAKRYEDQIWLVGDLAKSPL
ADQSFHVILNILSPSNYKEFKRILVKDGLVIKVVPRPNYLRELREALFDDKEKKVYKNDEIVSLFQKQLHLLDVFHLCYT
KELNNAELEHLVQMTPLAWSADQAHIDAFLHRDFAKITVDLDILVGVNRG

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=250, Percent_Identity=30.8, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 33317; Mature: 33186

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKTKSAERVSQFVAAFQCPLCKSAMRVEELKSLVCAKNHTFDFSKQGYVNLMTRPAHS
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHH
HYKKELFEARHKIIMESNVYTSMHETIAKVIKEYMDVSLNPFMVIDLGCGEGSHLQRILD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
ECMIPSMTGVGLDISKEGIAMAAKRYEDQIWLVGDLAKSPLADQSFHVILNILSPSNYKE
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHCCCCCCCCHHHEEEECCCCCHHH
FKRILVKDGLVIKVVPRPNYLRELREALFDDKEKKVYKNDEIVSLFQKQLHLLDVFHLCY
HHHHHHHCCCEEEEECCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TKELNNAELEHLVQMTPLAWSADQAHIDAFLHRDFAKITVDLDILVGVNRG
HHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHEEEEEEEEEECCCC
>Mature Secondary Structure 
TKKTKSAERVSQFVAAFQCPLCKSAMRVEELKSLVCAKNHTFDFSKQGYVNLMTRPAHS
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHH
HYKKELFEARHKIIMESNVYTSMHETIAKVIKEYMDVSLNPFMVIDLGCGEGSHLQRILD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
ECMIPSMTGVGLDISKEGIAMAAKRYEDQIWLVGDLAKSPLADQSFHVILNILSPSNYKE
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHCCCCCCCCHHHEEEECCCCCHHH
FKRILVKDGLVIKVVPRPNYLRELREALFDDKEKKVYKNDEIVSLFQKQLHLLDVFHLCY
HHHHHHHCCCEEEEECCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TKELNNAELEHLVQMTPLAWSADQAHIDAFLHRDFAKITVDLDILVGVNRG
HHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]