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The map label for this gene is sigD [H]

Identifier: 138894766

GI number: 138894766

Start: 1162038

End: 1162799

Strand: Direct

Name: sigD [H]

Synonym: GTNG_1100

Alternate gene names: 138894766

Gene position: 1162038-1162799 (Clockwise)

Preceding gene: 138894765

Following gene: 138894767

Centisome position: 32.73

GC content: 53.28

Gene sequence:

>762_bases
ATGGGGAGCGCGCTAACGCTAAAAGGAGACGAACAGAAATATTGGGACGACTGGGTCAACGGCCGCGACCGGCATGCGGC
CGAGGAGCTCGTACAGCGCTATATGCCGCTCGTTTCTTACCATGTGCAGCGGCTTTCCGCCACGCTGCCGAGTTCCGTGC
CGAAGGAAGAGCTGGTCAGCCTTGGGCTTGTCGGTTTGTACGATGCGCTTGAAAAGTTTGACCCATCGCGCGATTTGAAA
TTTGACACATATGCATCGTTTCGCATCCGCGGCGCCATCCTCGACGGTTTGCGCAAAGAAGATTGGCTGCCGCGCAGTGT
GCGCGACAAAGTAAAAAAAATTGAAGAGACGGCTGAGCGGCTTGAGCAACGGTACATGCGGTCGGTGACGGCGAAAGAAG
TTGCCGCCGAGCTCGGAATGACGGAAGAAGAAGTGCAGGCAGCGGTCAATGAAACGTTTTTTTCCAATTGGCTTTCGCTC
GGGCAGACAGCCTGTGAAGAGGACGATGAGCCGCTTGCGATCCGCGACGACCGCGCCGTACTTCCGGAAGAACAAGTCGT
CAAGCAAGAAATGATCGAAAAGCTGGCCGTGGGTATCGGACAGTTGAATGAAAAAGAGCAGCTTGTCATTAGCTTGTTTT
ACAAAGAGGAACTCACGTTTACGGAAATTGGGAGCATCCTGCAGCTGTCCACATCAAGAATTTCCCAAATTCATGCCAAA
GCGCTGTGGAAATTGCGCCGTTTTTTTGCTGAGGAGCAATGA

Upstream 100 bases:

>100_bases
GATCGAGTTTAATCCACAAACGGGCATGCTGTCCATTCGCACGGCTAGTCAAGAAATCAAAGAAATATAATGCCGTTTCC
ATAAGGCGAGGGGGAGAACG

Downstream 100 bases:

>100_bases
GGGGAAAGGAAGTGGAAAGGTGGATATTCAGCTGCCTGCGTTGCAAAGTGTGATGCCAAAAGTGCCGGAAGCGGGAGGTA
TGCATGCTGCTGCGCAGCAC

Product: RNA polymerase sigma factor SigD

Products: NA

Alternate protein names: Sigma-28 [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MGSALTLKGDEQKYWDDWVNGRDRHAAEELVQRYMPLVSYHVQRLSATLPSSVPKEELVSLGLVGLYDALEKFDPSRDLK
FDTYASFRIRGAILDGLRKEDWLPRSVRDKVKKIEETAERLEQRYMRSVTAKEVAAELGMTEEEVQAAVNETFFSNWLSL
GQTACEEDDEPLAIRDDRAVLPEEQVVKQEMIEKLAVGIGQLNEKEQLVISLFYKEELTFTEIGSILQLSTSRISQIHAK
ALWKLRRFFAEEQ

Sequences:

>Translated_253_residues
MGSALTLKGDEQKYWDDWVNGRDRHAAEELVQRYMPLVSYHVQRLSATLPSSVPKEELVSLGLVGLYDALEKFDPSRDLK
FDTYASFRIRGAILDGLRKEDWLPRSVRDKVKKIEETAERLEQRYMRSVTAKEVAAELGMTEEEVQAAVNETFFSNWLSL
GQTACEEDDEPLAIRDDRAVLPEEQVVKQEMIEKLAVGIGQLNEKEQLVISLFYKEELTFTEIGSILQLSTSRISQIHAK
ALWKLRRFFAEEQ
>Mature_252_residues
GSALTLKGDEQKYWDDWVNGRDRHAAEELVQRYMPLVSYHVQRLSATLPSSVPKEELVSLGLVGLYDALEKFDPSRDLKF
DTYASFRIRGAILDGLRKEDWLPRSVRDKVKKIEETAERLEQRYMRSVTAKEVAAELGMTEEEVQAAVNETFFSNWLSLG
QTACEEDDEPLAIRDDRAVLPEEQVVKQEMIEKLAVGIGQLNEKEQLVISLFYKEELTFTEIGSILQLSTSRISQIHAKA
LWKLRRFFAEEQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=225, Percent_Identity=37.3333333333333, Blast_Score=147, Evalue=7e-37,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29102; Mature: 28971

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSALTLKGDEQKYWDDWVNGRDRHAAEELVQRYMPLVSYHVQRLSATLPSSVPKEELVS
CCCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LGLVGLYDALEKFDPSRDLKFDTYASFRIRGAILDGLRKEDWLPRSVRDKVKKIEETAER
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LEQRYMRSVTAKEVAAELGMTEEEVQAAVNETFFSNWLSLGQTACEEDDEPLAIRDDRAV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
LPEEQVVKQEMIEKLAVGIGQLNEKEQLVISLFYKEELTFTEIGSILQLSTSRISQIHAK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALWKLRRFFAEEQ
HHHHHHHHHHCCC
>Mature Secondary Structure 
GSALTLKGDEQKYWDDWVNGRDRHAAEELVQRYMPLVSYHVQRLSATLPSSVPKEELVS
CCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LGLVGLYDALEKFDPSRDLKFDTYASFRIRGAILDGLRKEDWLPRSVRDKVKKIEETAER
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LEQRYMRSVTAKEVAAELGMTEEEVQAAVNETFFSNWLSLGQTACEEDDEPLAIRDDRAV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
LPEEQVVKQEMIEKLAVGIGQLNEKEQLVISLFYKEELTFTEIGSILQLSTSRISQIHAK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALWKLRRFFAEEQ
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612 [H]