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The map label for this gene is mtnD [H]

Identifier: 138894514

GI number: 138894514

Start: 919221

End: 919763

Strand: Direct

Name: mtnD [H]

Synonym: GTNG_0844

Alternate gene names: 138894514

Gene position: 919221-919763 (Clockwise)

Preceding gene: 138894513

Following gene: 138894516

Centisome position: 25.89

GC content: 45.67

Gene sequence:

>543_bases
ATGGCAGTGATTAAAGTGAAAAAAACGGGCCAAGTGATTGAAGGGGAAGACAATGTCCGCGCTTTTTTAAACAGCCAAGG
TGTTTTGTATGAGCATTGGGATATTACAAAACTACCGGAACATTTACGCGACAAATATGTGCTCACTGACGAAGAAAAAG
AGGAAATTTTGGCGACGTTTAAAGATGAGATCGAAGACTTGGCGGCGCGTCGTGGCTACAAAACATGGGATATCGTCGCC
CTGTCTGATGCGACTCCAAACTTGGAAGAGCTGCTGAAAAAATTTGAGCAAGTGCATATCCACACAGAGGATGAAGTGCG
CGCCATTACGGCCGGTCACGGCATTTTCATCATCAAAGGAGACAAAGAAACCGGTTATTTTGACGTCGAGTTGGAAGCGG
GCGATGTCATTTCTGTGCCGGAAGGAAATCCGCATTACTTCACATTGATGGATGACCGCCAAGTTGTTGCTGTCCGATTG
TTTATTGATCCGTCCGGTTGGGTCGCCCATCCGTACGAAGAAAAAGAAGAAGTGAATTCATAA

Upstream 100 bases:

>100_bases
GGGAGTTTTTGTTCAGCTGGCAGGTGAAGCGGCTATTATTACAACGGGCCGCTGTGCCGCTAGTTGACTAACCGATATAT
TTTTAAAGGGGGATTTGACG

Downstream 100 bases:

>100_bases
TAGTTGAGAGGCTCTCGCAACAAGGTGCCTTTTCTAAGTTATCTAGGTAGACATGGAAAACGCCTGCTTTCCATCGTCTT
CATAAAAGCGCATATAAAAA

Product: 5-methylthio-3-oxo-1-penten-1,2-diol dioxygenase

Products: NA

Alternate protein names: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; DHK-MTPene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); ARD'; Fe-ARD; Acireductone dioxygenase (Ni(2+)-requiring); ARD; Ni-ARD [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MAVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATFKDEIEDLAARRGYKTWDIVA
LSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKGDKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRL
FIDPSGWVAHPYEEKEEVNS

Sequences:

>Translated_180_residues
MAVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATFKDEIEDLAARRGYKTWDIVA
LSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKGDKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRL
FIDPSGWVAHPYEEKEEVNS
>Mature_179_residues
AVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATFKDEIEDLAARRGYKTWDIVAL
SDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKGDKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRLF
IDPSGWVAHPYEEKEEVNS

Specific function: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- me

COG id: COG1791

COG function: function code S; Uncharacterized conserved protein, contains double-stranded beta-helix domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acireductone dioxygenase (ARD) family [H]

Homologues:

Organism=Homo sapiens, GI171460928, Length=109, Percent_Identity=35.7798165137615, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323651, Length=113, Percent_Identity=30.0884955752212, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004313
- InterPro:   IPR011051
- InterPro:   IPR014710 [H]

Pfam domain/function: PF03079 ARD [H]

EC number: =1.13.11.54; =1.13.11.53 [H]

Molecular weight: Translated: 20597; Mature: 20466

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATF
CEEEEEECCCCEEECCCCEEEEECCCCEEEECCCCHHHHHHHCCCEEECCCHHHHHHHHH
KDEIEDLAARRGYKTWDIVALSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKG
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHEECCCCCCEEEEEECCEEEEEEC
DKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRLFIDPSGWVAHPYEEKEEVNS
CCCCCEEEEEECCCCEEECCCCCCEEEEEECCCEEEEEEEEECCCCCEECCCCCHHHCCC
>Mature Secondary Structure 
AVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATF
EEEEEECCCCEEECCCCEEEEECCCCEEEECCCCHHHHHHHCCCEEECCCHHHHHHHHH
KDEIEDLAARRGYKTWDIVALSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKG
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHEECCCCCCEEEEEECCEEEEEEC
DKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRLFIDPSGWVAHPYEEKEEVNS
CCCCCEEEEEECCCCEEECCCCCCEEEEEECCCEEEEEEEEECCCCCEECCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA