| Definition | Geobacillus thermodenitrificans NG80-2 chromosome, complete genome. |
|---|---|
| Accession | NC_009328 |
| Length | 3,550,319 |
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The map label for this gene is ytbE [H]
Identifier: 138894452
GI number: 138894452
Start: 854250
End: 855095
Strand: Direct
Name: ytbE [H]
Synonym: GTNG_0780
Alternate gene names: 138894452
Gene position: 854250-855095 (Clockwise)
Preceding gene: 138894451
Following gene: 138894453
Centisome position: 24.06
GC content: 37.47
Gene sequence:
>846_bases ATGGTTGCAAAAAATTTACAAGATACAACTACTTTGCATAACGGTGTAAAAATGCCCTGGTTTGGTATCGGAGTGTTTAA AGTAGAAGAAGGTCCCGAGTTAGTTAATGCTGTCAAAACAGCCATTAAACACGGTTATCGCAGTATTGATACAGCAGCTA TTTATGGAAATGAAGTGGGAGTCGGTCAAGGTATTCGTGAAGGGATGAAAGAAACTGGTATTTCTAGAGAAGAGCTATTT GTGACATCGAAAGTTTGGAATACCGATTTAGGATACGAATCTACGATTGCAGCCTATGAAACGAGCTTAACTAAACTTGG TTTAGAGTATCTAGATTTATATCTCATTCACTGGCCTGTAGAAGGTAAGTATAAAGATGCATGGAGAGCATTAGAGACGC TTTATAAAGAAGGACGAGTAAAAGCTATTGGAGTAAGTAATTTTCAAATCCATCATCTTGAAGACTTGATGAAAGACGCA GAAATTAAACCAATGGTGAATCAAGTGGAATACCATCCGCGTTTAACCCAAAAAGAACTTCAGTCGTTTTGTCAGGAACA TGGGATTCAGCTAGAAGCTTGGTCTCCATTAATGCAAGGTCAATTACTAAATAATGAGGTTTTACAAGAGATCGCAAATA AGTATAACAAATCTGTTGCCCAAGTCATTTTACGTTGGGACTTACAAAACGGCGTTGTAACCATTCCGAAAACTACAAAA GAACATCGCATTGTTGAAAACGCATCGGTATTTGATTTTGAGTTAACGGAAGAAGATATGAAGAAGATTGATGGTCTGAA TCAGAATCATCGAGTCGGTCCAGACCCAGACAATTTTGATTTTTAA
Upstream 100 bases:
>100_bases AAAATATAAAATATTGATTAGAGGGAGGTGATGGATACTTACATAGACAGCAACACTGTTTAAATCCAATTTCAATTCTT AATCATTAGGAGGTCAAGTT
Downstream 100 bases:
>100_bases CATGTTCGCCGAGAAGGACCCATCTCTTAATGGCTCGTAAGGGGGAGAGGCTCATAAACGGAAAAGAAGTACCTTATGGG ACTAAAGGAAGCTCAAGACT
Product: 2,5-diketo-D-gluconic acid reductase
Products: 2-keto-L-gulonate; NADP [C]
Alternate protein names: NA
Number of amino acids: Translated: 281; Mature: 281
Protein sequence:
>281_residues MVAKNLQDTTTLHNGVKMPWFGIGVFKVEEGPELVNAVKTAIKHGYRSIDTAAIYGNEVGVGQGIREGMKETGISREELF VTSKVWNTDLGYESTIAAYETSLTKLGLEYLDLYLIHWPVEGKYKDAWRALETLYKEGRVKAIGVSNFQIHHLEDLMKDA EIKPMVNQVEYHPRLTQKELQSFCQEHGIQLEAWSPLMQGQLLNNEVLQEIANKYNKSVAQVILRWDLQNGVVTIPKTTK EHRIVENASVFDFELTEEDMKKIDGLNQNHRVGPDPDNFDF
Sequences:
>Translated_281_residues MVAKNLQDTTTLHNGVKMPWFGIGVFKVEEGPELVNAVKTAIKHGYRSIDTAAIYGNEVGVGQGIREGMKETGISREELF VTSKVWNTDLGYESTIAAYETSLTKLGLEYLDLYLIHWPVEGKYKDAWRALETLYKEGRVKAIGVSNFQIHHLEDLMKDA EIKPMVNQVEYHPRLTQKELQSFCQEHGIQLEAWSPLMQGQLLNNEVLQEIANKYNKSVAQVILRWDLQNGVVTIPKTTK EHRIVENASVFDFELTEEDMKKIDGLNQNHRVGPDPDNFDF >Mature_281_residues MVAKNLQDTTTLHNGVKMPWFGIGVFKVEEGPELVNAVKTAIKHGYRSIDTAAIYGNEVGVGQGIREGMKETGISREELF VTSKVWNTDLGYESTIAAYETSLTKLGLEYLDLYLIHWPVEGKYKDAWRALETLYKEGRVKAIGVSNFQIHHLEDLMKDA EIKPMVNQVEYHPRLTQKELQSFCQEHGIQLEAWSPLMQGQLLNNEVLQEIANKYNKSVAQVILRWDLQNGVVTIPKTTK EHRIVENASVFDFELTEEDMKKIDGLNQNHRVGPDPDNFDF
Specific function: Reduction [C]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI24497577, Length=291, Percent_Identity=41.5807560137457, Blast_Score=226, Evalue=1e-59, Organism=Homo sapiens, GI5174391, Length=291, Percent_Identity=41.5807560137457, Blast_Score=226, Evalue=1e-59, Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=40.9556313993174, Blast_Score=208, Evalue=4e-54, Organism=Homo sapiens, GI291291012, Length=308, Percent_Identity=38.961038961039, Blast_Score=194, Evalue=1e-49, Organism=Homo sapiens, GI93277124, Length=304, Percent_Identity=38.1578947368421, Blast_Score=192, Evalue=4e-49, Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=38.1756756756757, Blast_Score=191, Evalue=5e-49, Organism=Homo sapiens, GI310109922, Length=293, Percent_Identity=38.9078498293515, Blast_Score=191, Evalue=6e-49, Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=38.6440677966102, Blast_Score=188, Evalue=4e-48, Organism=Homo sapiens, GI5453543, Length=313, Percent_Identity=37.3801916932907, Blast_Score=187, Evalue=6e-48, Organism=Homo sapiens, GI310109920, Length=313, Percent_Identity=37.3801916932907, Blast_Score=187, Evalue=7e-48, Organism=Homo sapiens, GI45446745, Length=313, Percent_Identity=37.3801916932907, Blast_Score=187, Evalue=1e-47, Organism=Homo sapiens, GI4503285, Length=313, Percent_Identity=37.3801916932907, Blast_Score=187, Evalue=1e-47, Organism=Homo sapiens, GI4502049, Length=293, Percent_Identity=40.9556313993174, Blast_Score=185, Evalue=5e-47, Organism=Homo sapiens, GI24497583, Length=296, Percent_Identity=36.8243243243243, Blast_Score=181, Evalue=9e-46, Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=36.3636363636364, Blast_Score=163, Evalue=1e-40, Organism=Homo sapiens, GI300116271, Length=274, Percent_Identity=36.1313868613139, Blast_Score=160, Evalue=1e-39, Organism=Homo sapiens, GI310109924, Length=169, Percent_Identity=38.4615384615385, Blast_Score=109, Evalue=3e-24, Organism=Homo sapiens, GI310109926, Length=117, Percent_Identity=41.025641025641, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI207028673, Length=117, Percent_Identity=41.025641025641, Blast_Score=89, Evalue=4e-18, Organism=Escherichia coli, GI87082198, Length=272, Percent_Identity=44.4852941176471, Blast_Score=246, Evalue=8e-67, Organism=Escherichia coli, GI1786400, Length=265, Percent_Identity=36.2264150943396, Blast_Score=170, Evalue=9e-44, Organism=Escherichia coli, GI1788081, Length=274, Percent_Identity=32.8467153284672, Blast_Score=128, Evalue=3e-31, Organism=Escherichia coli, GI1788070, Length=309, Percent_Identity=23.3009708737864, Blast_Score=79, Evalue=2e-16, Organism=Escherichia coli, GI1787674, Length=274, Percent_Identity=25.5474452554745, Blast_Score=72, Evalue=5e-14, Organism=Escherichia coli, GI87081735, Length=282, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=7e-12, Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=37.3333333333333, Blast_Score=208, Evalue=2e-54, Organism=Caenorhabditis elegans, GI71998625, Length=273, Percent_Identity=42.1245421245421, Blast_Score=206, Evalue=8e-54, Organism=Caenorhabditis elegans, GI17537077, Length=298, Percent_Identity=39.261744966443, Blast_Score=197, Evalue=5e-51, Organism=Caenorhabditis elegans, GI17537075, Length=307, Percent_Identity=38.4364820846906, Blast_Score=194, Evalue=4e-50, Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=34.4322344322344, Blast_Score=185, Evalue=2e-47, Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=37.1134020618557, Blast_Score=179, Evalue=2e-45, Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=35.4243542435424, Blast_Score=176, Evalue=1e-44, Organism=Caenorhabditis elegans, GI17538386, Length=275, Percent_Identity=35.6363636363636, Blast_Score=172, Evalue=2e-43, Organism=Caenorhabditis elegans, GI17537079, Length=314, Percent_Identity=33.7579617834395, Blast_Score=170, Evalue=9e-43, Organism=Caenorhabditis elegans, GI17562292, Length=277, Percent_Identity=31.0469314079422, Blast_Score=160, Evalue=1e-39, Organism=Caenorhabditis elegans, GI17566692, Length=286, Percent_Identity=31.8181818181818, Blast_Score=157, Evalue=7e-39, Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=33.5793357933579, Blast_Score=155, Evalue=3e-38, Organism=Caenorhabditis elegans, GI17550246, Length=77, Percent_Identity=42.8571428571429, Blast_Score=65, Evalue=4e-11, Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=36.9085173501577, Blast_Score=197, Evalue=1e-51, Organism=Saccharomyces cerevisiae, GI6324694, Length=298, Percent_Identity=37.9194630872483, Blast_Score=188, Evalue=1e-48, Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=38.0769230769231, Blast_Score=186, Evalue=3e-48, Organism=Saccharomyces cerevisiae, GI6320576, Length=299, Percent_Identity=36.1204013377926, Blast_Score=183, Evalue=3e-47, Organism=Saccharomyces cerevisiae, GI6319625, Length=297, Percent_Identity=35.3535353535354, Blast_Score=173, Evalue=2e-44, Organism=Saccharomyces cerevisiae, GI6320079, Length=291, Percent_Identity=36.0824742268041, Blast_Score=152, Evalue=4e-38, Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=39.4557823129252, Blast_Score=209, Evalue=2e-54, Organism=Drosophila melanogaster, GI24663317, Length=298, Percent_Identity=37.5838926174497, Blast_Score=201, Evalue=4e-52, Organism=Drosophila melanogaster, GI24657054, Length=291, Percent_Identity=38.8316151202749, Blast_Score=198, Evalue=4e-51, Organism=Drosophila melanogaster, GI20129731, Length=294, Percent_Identity=37.4149659863946, Blast_Score=196, Evalue=2e-50, Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=35.3333333333333, Blast_Score=184, Evalue=6e-47, Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=33.125, Blast_Score=176, Evalue=1e-44, Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=34.3642611683849, Blast_Score=174, Evalue=6e-44, Organism=Drosophila melanogaster, GI45553081, Length=297, Percent_Identity=33.3333333333333, Blast_Score=173, Evalue=9e-44, Organism=Drosophila melanogaster, GI24662785, Length=291, Percent_Identity=39.8625429553265, Blast_Score=170, Evalue=1e-42, Organism=Drosophila melanogaster, GI24662781, Length=293, Percent_Identity=39.5904436860068, Blast_Score=169, Evalue=2e-42, Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=33.7209302325581, Blast_Score=155, Evalue=4e-38,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: NA
Molecular weight: Translated: 32036; Mature: 32036
Theoretical pI: Translated: 5.24; Mature: 5.24
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVAKNLQDTTTLHNGVKMPWFGIGVFKVEEGPELVNAVKTAIKHGYRSIDTAAIYGNEVG CCCCCCCHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHCHHEEECCCCC VGQGIREGMKETGISREELFVTSKVWNTDLGYESTIAAYETSLTKLGLEYLDLYLIHWPV CCHHHHHHHHHHCCCHHHEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCC EGKYKDAWRALETLYKEGRVKAIGVSNFQIHHLEDLMKDAEIKPMVNQVEYHPRLTQKEL CCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHH QSFCQEHGIQLEAWSPLMQGQLLNNEVLQEIANKYNKSVAQVILRWDLQNGVVTIPKTTK HHHHHHCCCEEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC EHRIVENASVFDFELTEEDMKKIDGLNQNHRVGPDPDNFDF HHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCC >Mature Secondary Structure MVAKNLQDTTTLHNGVKMPWFGIGVFKVEEGPELVNAVKTAIKHGYRSIDTAAIYGNEVG CCCCCCCHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHCHHEEECCCCC VGQGIREGMKETGISREELFVTSKVWNTDLGYESTIAAYETSLTKLGLEYLDLYLIHWPV CCHHHHHHHHHHCCCHHHEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCC EGKYKDAWRALETLYKEGRVKAIGVSNFQIHHLEDLMKDAEIKPMVNQVEYHPRLTQKEL CCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHH QSFCQEHGIQLEAWSPLMQGQLLNNEVLQEIANKYNKSVAQVILRWDLQNGVVTIPKTTK HHHHHHCCCEEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC EHRIVENASVFDFELTEEDMKKIDGLNQNHRVGPDPDNFDF HHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: 2,5-diketo-D-gluconate; NADPH [C]
Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]