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The map label for this gene is dnaN [H]

Identifier: 138893681

GI number: 138893681

Start: 1733

End: 2869

Strand: Direct

Name: dnaN [H]

Synonym: GTNG_0002

Alternate gene names: 138893681

Gene position: 1733-2869 (Clockwise)

Preceding gene: 138893680

Following gene: 138893682

Centisome position: 0.05

GC content: 46.35

Gene sequence:

>1137_bases
GTGAACATTTCCATTGATCGCGAAGCGCTGGCAAAAAGCGTCCAAGACGTAATGAAAGCTGTATCGACAAGAACAACGAT
CCCGATTTTAACCGGAATTAAGTTAACAGCAAACGCCCATGGAATGACGTTGACCGGAAGTGACTCTGACATTTCGATTG
AGTCGTTTATTCCGCTCGAAAAAGAGGGACGCTTGCTTGTTGACGTAAAAAGACCCGGAAGCATCGTGCTGCAAGCGCGC
TTTTTTGCCGAAATCGTCAAAAAGCTGCCTCAACAAACCATTGAGATTGAAACCGAGGACAACTTCTTGACTGTCATCCG
CTCAGGACACTCAGAATTCCGTCTCAATGGCTTAAACGCTGAGGAATATCCACGTTTGCCGCAAATTGAAGAGGAAAACG
TGTTTCAAATTCCGGCTGATTTATTAAAAACGCTGATCCGACAAACAGTGTTTGCTGTATCAACATCGGAAACACGCCCG
ATCTTGACAGGTGTCAACTGGAAAGTCGAAAACGGCGAATTGATTTGCACGGCGACAGATAGTCACCGCCTAGCGATGCG
CAAAGTGAAAATCGAAGCGGAAAACGCGATTTCATATAACGTCGTCATTCCAGGGAAAAGCCTCAACGAGCTGAGCAAAA
TTTTAGATGACGGCAATGCCCCAGTCAGTATCGTTATCACGGCCAACCAAGTGTTGTTTAAGGCGGAACATCTTCTGTTC
TTTTCCCGATTGCTCGACGGAAATTACCCAGAGACAGCCCGCTTGATCCCAACCGACAGCAAAACGAACATGGTCGTCAA
TGCAAAAGAATTTTTGCAAGCGATCGATCGGGCATCCTTGCTTGCAAGGGAAGGAAGAAACAATGTCGTGAAACTGACGA
CGCTCCCTGGAGGATTACTTGAGATTTCTTCGATTTCTCCGGAGATCGGAAAAGTGACTGAGCAGTTGCAAACCGAGTCG
ATTGAAGGCGAAGAATTAAACATTTCCTTCAGTGCAAAATACATGATGGATGCGCTGCGGGCGCTTGATGGCAACGATAT
TCAAATCAGTTTCACGGGGGCAATGCGTCCGTTCTTGTTGCGTCCACTTCATACAGACTCAATGCTTCAGCTTATTTTGC
CGGTAAGAACATATTGA

Upstream 100 bases:

>100_bases
ATCCACATACTAACAAGCCATATGACTATTACTACGAGTTTTTATTTTTATATTTTTATAATTTATTTCCGAACATCAAC
TAAACGGGGAGGAACGACAA

Downstream 100 bases:

>100_bases
TAACCCCTTAATAAAGCTAGCTTTACGCGCATAACGGCTGTGCTGTCTTAAACATGACTTTTTCCGTGACCGATGAGAAG
GATGGGAATCCCTTTGACTG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNISIDREALAKSVQDVMKAVSTRTTIPILTGIKLTANAHGMTLTGSDSDISIESFIPLEKEGRLLVDVKRPGSIVLQAR
FFAEIVKKLPQQTIEIETEDNFLTVIRSGHSEFRLNGLNAEEYPRLPQIEEENVFQIPADLLKTLIRQTVFAVSTSETRP
ILTGVNWKVENGELICTATDSHRLAMRKVKIEAENAISYNVVIPGKSLNELSKILDDGNAPVSIVITANQVLFKAEHLLF
FSRLLDGNYPETARLIPTDSKTNMVVNAKEFLQAIDRASLLAREGRNNVVKLTTLPGGLLEISSISPEIGKVTEQLQTES
IEGEELNISFSAKYMMDALRALDGNDIQISFTGAMRPFLLRPLHTDSMLQLILPVRTY

Sequences:

>Translated_378_residues
MNISIDREALAKSVQDVMKAVSTRTTIPILTGIKLTANAHGMTLTGSDSDISIESFIPLEKEGRLLVDVKRPGSIVLQAR
FFAEIVKKLPQQTIEIETEDNFLTVIRSGHSEFRLNGLNAEEYPRLPQIEEENVFQIPADLLKTLIRQTVFAVSTSETRP
ILTGVNWKVENGELICTATDSHRLAMRKVKIEAENAISYNVVIPGKSLNELSKILDDGNAPVSIVITANQVLFKAEHLLF
FSRLLDGNYPETARLIPTDSKTNMVVNAKEFLQAIDRASLLAREGRNNVVKLTTLPGGLLEISSISPEIGKVTEQLQTES
IEGEELNISFSAKYMMDALRALDGNDIQISFTGAMRPFLLRPLHTDSMLQLILPVRTY
>Mature_378_residues
MNISIDREALAKSVQDVMKAVSTRTTIPILTGIKLTANAHGMTLTGSDSDISIESFIPLEKEGRLLVDVKRPGSIVLQAR
FFAEIVKKLPQQTIEIETEDNFLTVIRSGHSEFRLNGLNAEEYPRLPQIEEENVFQIPADLLKTLIRQTVFAVSTSETRP
ILTGVNWKVENGELICTATDSHRLAMRKVKIEAENAISYNVVIPGKSLNELSKILDDGNAPVSIVITANQVLFKAEHLLF
FSRLLDGNYPETARLIPTDSKTNMVVNAKEFLQAIDRASLLAREGRNNVVKLTTLPGGLLEISSISPEIGKVTEQLQTES
IEGEELNISFSAKYMMDALRALDGNDIQISFTGAMRPFLLRPLHTDSMLQLILPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=28.3819628647215, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41943; Mature: 41943

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISIDREALAKSVQDVMKAVSTRTTIPILTGIKLTANAHGMTLTGSDSDISIESFIPLE
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEHHHCCCCC
KEGRLLVDVKRPGSIVLQARFFAEIVKKLPQQTIEIETEDNFLTVIRSGHSEFRLNGLNA
CCCCEEEEEECCCCEEEEHHHHHHHHHHCCHHEEEEECCCCEEEEEECCCCEEEEECCCH
EEYPRLPQIEEENVFQIPADLLKTLIRQTVFAVSTSETRPILTGVNWKVENGELICTATD
HHCCCCCCCCCCCEEECCHHHHHHHHHHHHEEEECCCCCCEEECCEEEEECCEEEEEECC
SHRLAMRKVKIEAENAISYNVVIPGKSLNELSKILDDGNAPVSIVITANQVLFKAEHLLF
CCCEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
FSRLLDGNYPETARLIPTDSKTNMVVNAKEFLQAIDRASLLAREGRNNVVKLTTLPGGLL
HHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
EISSISPEIGKVTEQLQTESIEGEELNISFSAKYMMDALRALDGNDIQISFTGAMRPFLL
EECCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCCHHC
RPLHTDSMLQLILPVRTY
CCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNISIDREALAKSVQDVMKAVSTRTTIPILTGIKLTANAHGMTLTGSDSDISIESFIPLE
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEHHHCCCCC
KEGRLLVDVKRPGSIVLQARFFAEIVKKLPQQTIEIETEDNFLTVIRSGHSEFRLNGLNA
CCCCEEEEEECCCCEEEEHHHHHHHHHHCCHHEEEEECCCCEEEEEECCCCEEEEECCCH
EEYPRLPQIEEENVFQIPADLLKTLIRQTVFAVSTSETRPILTGVNWKVENGELICTATD
HHCCCCCCCCCCCEEECCHHHHHHHHHHHHEEEECCCCCCEEECCEEEEECCEEEEEECC
SHRLAMRKVKIEAENAISYNVVIPGKSLNELSKILDDGNAPVSIVITANQVLFKAEHLLF
CCCEEEEEEEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
FSRLLDGNYPETARLIPTDSKTNMVVNAKEFLQAIDRASLLAREGRNNVVKLTTLPGGLL
HHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
EISSISPEIGKVTEQLQTESIEGEELNISFSAKYMMDALRALDGNDIQISFTGAMRPFLL
EECCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCCHHC
RPLHTDSMLQLILPVRTY
CCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]