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The map label for this gene is trpCF [H]

Identifier: 134302793

GI number: 134302793

Start: 1888679

End: 1890040

Strand: Reverse

Name: trpCF [H]

Synonym: FTW_2017

Alternate gene names: 134302793

Gene position: 1890040-1888679 (Counterclockwise)

Preceding gene: 134302794

Following gene: 134302791

Centisome position: 99.56

GC content: 31.2

Gene sequence:

>1362_bases
ATGGAAACTATTTTAGCAAAAATCGTTGAAGCAAAAAGAAAGTGGCTTTTTGCAAAAAAAAGACTATTTCCACTAGATGT
ATTTAAAAAAGAAGTTGTCAAAACAGATAGAGATTTCTATCAAGCTTTGGAATCTGATAAAGCTGTATTTATTTTAGAAT
GTAAAAAAGGCTCTCCTTCTAAAGGAATTATTCGTAAAAAGTTTGATCTAAATGAAATTGCAGCTGTATACAAAAATTAT
GCAAATGTGATATCTGTACTTACAGATGAAGAATTTTTTATGGGTAGCTTTGCAAATCTAGAGATTGTTAGAAAGCAAGT
AACACAACCTATGCTTTGTAAAGATTTTATAATAGATGAATATCAAATATTCTTAGCTAGGCATTATCAAGCTGATGCTG
TGTTACTTATGCTTTCAGTACTTGATGATAGTGAGTATAAAAAATTAGCAAAAGTTGCTAATCGTCTAGGTATGGGTATA
TTAACCGAAGTTAGTAACGAAGAAGAGCTTGTAAGAGCTATAAGCTTAAAAGCAAAAGTTATAGGTATTAATAATCGTAA
TCTAAGAGATCTTTCTATAGATTTAAATACTACGCGTCTTTTAGCTCCGAAAATACCTAAAGGAACAATTATTATTTCAG
AATCAGGGATCTATAAAAATCAAGAAATACGTGATCTAAGAAATTATGTAAATGGCTTTTTGATAGGAAGTTCTATTATG
GGTGAACGTAACCTTGAACTTGCAGTTAGAAAGCTTGTTTATGGTTTTAATAAGGTTTGCGGCCTTACTTCTGTTGAGGA
TGCTCAAAAAGCATATGATGCAGGAGCAGTCTATGGTGGTTTGATTTTTGTTGAGAAATCTCCTCGTTATGTAGATTTTG
CCATGGCAAAACAGATAGTAAAAAAAGTTAGGCTAAATTATGTAGGTGTTTTTGCTGACGCTAAAATAGAAGATGTTGTA
GATACCTGCTATGCATTAAAATTAAGTGCTGTGCAGTTGCATGGTGATGAAAATCAAGAGTATATAGATACTCTAAAATC
AAAGCTACATAGAAACTGTCAAATATGGAAAGCATATCCAGTAGATAAGACTCTTCCAAGTTTTTTTGCTAACGTAGATT
ATCATTTATTAGATGCCCAAGTAGATGGTAAATCAGGTGGTACAGGAAAAACATTTGATTGGAGTCTTATCAAAGACAGA
AAAAATATAATTCTTGCTGGCGGTCTGAATGCTAAAAATATAGCAAAAGCAATAGAATTGAAGTGTTCAGCTTATGATAT
TAATTCTGGTGTAGAATCTGAACCAGGACAAAAAGATCAAATCAAACTAAATGAAGTATTTGAAATAATTAGAAGTTATT
AA

Upstream 100 bases:

>100_bases
CTAAAATAAATAATCTATAAATTATCAAAATACAGAAAAATTCAACTTTTATTCTTTTTTCGTTTAAAATACTAGATAAA
ATCAAATAATCCCTAATAAT

Downstream 100 bases:

>100_bases
GATTTTATTGATATCTTTTTAGTAGTATTATCTTTTTCTTTTTTAGGGATGTTCAAGCTTAGTACACCATTACTGTATTT
AGCTTCTATTTTGTCACTGT

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
METILAKIVEAKRKWLFAKKRLFPLDVFKKEVVKTDRDFYQALESDKAVFILECKKGSPSKGIIRKKFDLNEIAAVYKNY
ANVISVLTDEEFFMGSFANLEIVRKQVTQPMLCKDFIIDEYQIFLARHYQADAVLLMLSVLDDSEYKKLAKVANRLGMGI
LTEVSNEEELVRAISLKAKVIGINNRNLRDLSIDLNTTRLLAPKIPKGTIIISESGIYKNQEIRDLRNYVNGFLIGSSIM
GERNLELAVRKLVYGFNKVCGLTSVEDAQKAYDAGAVYGGLIFVEKSPRYVDFAMAKQIVKKVRLNYVGVFADAKIEDVV
DTCYALKLSAVQLHGDENQEYIDTLKSKLHRNCQIWKAYPVDKTLPSFFANVDYHLLDAQVDGKSGGTGKTFDWSLIKDR
KNIILAGGLNAKNIAKAIELKCSAYDINSGVESEPGQKDQIKLNEVFEIIRSY

Sequences:

>Translated_453_residues
METILAKIVEAKRKWLFAKKRLFPLDVFKKEVVKTDRDFYQALESDKAVFILECKKGSPSKGIIRKKFDLNEIAAVYKNY
ANVISVLTDEEFFMGSFANLEIVRKQVTQPMLCKDFIIDEYQIFLARHYQADAVLLMLSVLDDSEYKKLAKVANRLGMGI
LTEVSNEEELVRAISLKAKVIGINNRNLRDLSIDLNTTRLLAPKIPKGTIIISESGIYKNQEIRDLRNYVNGFLIGSSIM
GERNLELAVRKLVYGFNKVCGLTSVEDAQKAYDAGAVYGGLIFVEKSPRYVDFAMAKQIVKKVRLNYVGVFADAKIEDVV
DTCYALKLSAVQLHGDENQEYIDTLKSKLHRNCQIWKAYPVDKTLPSFFANVDYHLLDAQVDGKSGGTGKTFDWSLIKDR
KNIILAGGLNAKNIAKAIELKCSAYDINSGVESEPGQKDQIKLNEVFEIIRSY
>Mature_453_residues
METILAKIVEAKRKWLFAKKRLFPLDVFKKEVVKTDRDFYQALESDKAVFILECKKGSPSKGIIRKKFDLNEIAAVYKNY
ANVISVLTDEEFFMGSFANLEIVRKQVTQPMLCKDFIIDEYQIFLARHYQADAVLLMLSVLDDSEYKKLAKVANRLGMGI
LTEVSNEEELVRAISLKAKVIGINNRNLRDLSIDLNTTRLLAPKIPKGTIIISESGIYKNQEIRDLRNYVNGFLIGSSIM
GERNLELAVRKLVYGFNKVCGLTSVEDAQKAYDAGAVYGGLIFVEKSPRYVDFAMAKQIVKKVRLNYVGVFADAKIEDVV
DTCYALKLSAVQLHGDENQEYIDTLKSKLHRNCQIWKAYPVDKTLPSFFANVDYHLLDAQVDGKSGGTGKTFDWSLIKDR
KNIILAGGLNAKNIAKAIELKCSAYDINSGVESEPGQKDQIKLNEVFEIIRSY

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=456, Percent_Identity=55.7017543859649, Blast_Score=502, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6322638, Length=228, Percent_Identity=38.5964912280702, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320210, Length=206, Percent_Identity=32.0388349514563, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 51167; Mature: 51167

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METILAKIVEAKRKWLFAKKRLFPLDVFKKEVVKTDRDFYQALESDKAVFILECKKGSPS
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCCC
KGIIRKKFDLNEIAAVYKNYANVISVLTDEEFFMGSFANLEIVRKQVTQPMLCKDFIIDE
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
YQIFLARHYQADAVLLMLSVLDDSEYKKLAKVANRLGMGILTEVSNEEELVRAISLKAKV
HHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHEEEE
IGINNRNLRDLSIDLNTTRLLAPKIPKGTIIISESGIYKNQEIRDLRNYVNGFLIGSSIM
EECCCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCHHC
GERNLELAVRKLVYGFNKVCGLTSVEDAQKAYDAGAVYGGLIFVEKSPRYVDFAMAKQIV
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEHHHHHHHHH
KKVRLNYVGVFADAKIEDVVDTCYALKLSAVQLHGDENQEYIDTLKSKLHRNCQIWKAYP
HHHHHCEEEEEECCHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECC
VDKTLPSFFANVDYHLLDAQVDGKSGGTGKTFDWSLIKDRKNIILAGGLNAKNIAKAIEL
CCHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEHHEECCCCCEEEECCCCHHHHHHHHHE
KCSAYDINSGVESEPGQKDQIKLNEVFEIIRSY
EEEEEECCCCCCCCCCCCCCEEHHHHHHHHHCC
>Mature Secondary Structure
METILAKIVEAKRKWLFAKKRLFPLDVFKKEVVKTDRDFYQALESDKAVFILECKKGSPS
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCCC
KGIIRKKFDLNEIAAVYKNYANVISVLTDEEFFMGSFANLEIVRKQVTQPMLCKDFIIDE
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
YQIFLARHYQADAVLLMLSVLDDSEYKKLAKVANRLGMGILTEVSNEEELVRAISLKAKV
HHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHEEEE
IGINNRNLRDLSIDLNTTRLLAPKIPKGTIIISESGIYKNQEIRDLRNYVNGFLIGSSIM
EECCCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCHHC
GERNLELAVRKLVYGFNKVCGLTSVEDAQKAYDAGAVYGGLIFVEKSPRYVDFAMAKQIV
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEHHHHHHHHH
KKVRLNYVGVFADAKIEDVVDTCYALKLSAVQLHGDENQEYIDTLKSKLHRNCQIWKAYP
HHHHHCEEEEEECCHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECC
VDKTLPSFFANVDYHLLDAQVDGKSGGTGKTFDWSLIKDRKNIILAGGLNAKNIAKAIEL
CCHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEHHEECCCCCEEEECCCCHHHHHHHHHE
KCSAYDINSGVESEPGQKDQIKLNEVFEIIRSY
EEEEEECCCCCCCCCCCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]