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The map label for this gene is prfB [H]

Identifier: 134302701

GI number: 134302701

Start: 1782688

End: 1783671

Strand: Reverse

Name: prfB [H]

Synonym: FTW_1900

Alternate gene names: 134302701

Gene position: 1783671-1782688 (Counterclockwise)

Preceding gene: 134302703

Following gene: 134302700

Centisome position: 93.95

GC content: 38.82

Gene sequence:

>984_bases
ATGGAGCTAGAAGATGGTTCTATTTGGGATAATCCAGAGTATGCACAAAACTTGGGTAAACAAAAAGTAGAACTTGAGAA
CGTTGTACACAATTGTGAGTATATCTCAGAAACTCTAGAAACTTTAAGTGAACTGCTTGAGCTTGGTGAAGAAGATGAAT
CTTTAATGCAAGAAATTGCTAAAGATACACAAAATGTTGCTAGTGAAATTGAGAAGCTTGAGTTTCGTCGCATGTTTTCA
GGTAAAATGGATGCTAATAACGCCTTTTTGGATATTCAGTCAGGTTCTGGAGGTACAGAGGCGCAAGATTGGGCAGAGAT
GCTGATGCGGATGTATATGCGTTGGGCAGATAGTCACGGCTTCAAGGTAACCGTTGATGATGTATCAGATGGTGATGTTG
CGGGTATAAAAGGCTGCACCTTAAAAATAGAGGGAGAGTATGCTTATGGTTGGTTGCGTACCGAAACTGGTATTCATAGG
TTAGTACGCAAATCACCTTTTGACTCAAACAGTAAACGTCATACATCTTTTGCATCGGTGTTTATCTCACCTGAGGTTGA
TGATGATATTGATATTGATATTGAGATAAATCCTACTGATTTACGAGTCGATACTTATCGTGCATCAGGTGCTGGTGGTC
AGCATGTCAACAAAACAGACTCTGCTGTGAGAATAACGCATATTCCGACAAATATTGTGGTACAAAGTCAAAGTGATAGA
TCTCAGCATAAAAATAGAGATAATGCCATGAAACAGCTTAAGTCAAAGCTATATGAAATGGAGTTACAAAAGCGTAATGC
TGAAAAGAATGCACTCGAAGACTCAAAGGCAGATATTGGTTGGGGTAGTCAGATTCGTTCGTACGTGTTGGATCAGTCAC
GAATTAAGGATTTAAGAACAGGAGTTGAGAATACTAATACACAAGCGGTATTAGATGGTGATTTGGATAAGTTTATTGAG
GCTAGCTTAAAAAGTGGGTTATAA

Upstream 100 bases:

>100_bases
AATTATAAGCTTGTATTAAAAGATCTAACGGCCCGTATTCAATCTTTACGGGACTATCTTTGACTATGATACTAAGAAAG
AAAAGCTAACAGAAGTCTTA

Downstream 100 bases:

>100_bases
TAAATAGATTTTATAAAAAGTATGCAAAACTGCCTAGCACTACGCTATTGTAGTTTAAAACAAATGAAAAATAAAAAACT
CGCCAATTTGAAAATTGATT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIAKDTQNVASEIEKLEFRRMFS
GKMDANNAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHGFKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHR
LVRKSPFDSNSKRHTSFASVFISPEVDDDIDIDIEINPTDLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDR
SQHKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGVENTNTQAVLDGDLDKFIE
ASLKSGL

Sequences:

>Translated_327_residues
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIAKDTQNVASEIEKLEFRRMFS
GKMDANNAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHGFKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHR
LVRKSPFDSNSKRHTSFASVFISPEVDDDIDIDIEINPTDLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDR
SQHKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGVENTNTQAVLDGDLDKFIE
ASLKSGL
>Mature_327_residues
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIAKDTQNVASEIEKLEFRRMFS
GKMDANNAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHGFKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHR
LVRKSPFDSNSKRHTSFASVFISPEVDDDIDIDIEINPTDLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDR
SQHKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGVENTNTQAVLDGDLDKFIE
ASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=336, Percent_Identity=32.1428571428571, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI166795303, Length=245, Percent_Identity=35.9183673469388, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI166795305, Length=188, Percent_Identity=34.5744680851064, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI2367172, Length=328, Percent_Identity=66.4634146341463, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=40.9090909090909, Blast_Score=184, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=258, Percent_Identity=29.4573643410853, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=299, Percent_Identity=34.1137123745819, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19921226, Length=253, Percent_Identity=35.9683794466403, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36853; Mature: 36853

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIA
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KDTQNVASEIEKLEFRRMFSGKMDANNAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
FKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHRLVRKSPFDSNSKRHTSFASV
CEEEECCCCCCCCCCCCCEEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEE
FISPEVDDDIDIDIEINPTDLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDR
EECCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHH
SQHKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRT
HHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GVENTNTQAVLDGDLDKFIEASLKSGL
CCCCCCCCEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIA
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KDTQNVASEIEKLEFRRMFSGKMDANNAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
FKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHRLVRKSPFDSNSKRHTSFASV
CEEEECCCCCCCCCCCCCEEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEE
FISPEVDDDIDIDIEINPTDLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDR
EECCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHH
SQHKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRT
HHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GVENTNTQAVLDGDLDKFIEASLKSGL
CCCCCCCCEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA