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The map label for this gene is bioD [H]

Identifier: 134302630

GI number: 134302630

Start: 806718

End: 807395

Strand: Reverse

Name: bioD [H]

Synonym: FTW_2081

Alternate gene names: 134302630

Gene position: 807395-806718 (Counterclockwise)

Preceding gene: 134301846

Following gene: 134301843

Centisome position: 42.53

GC content: 30.53

Gene sequence:

>678_bases
ATGAAAAAGTTTTTTATCATTGGTACGGATACCGAAGTAGGTAAAACATATATATCAACCAAGCTTATTGAAGTTTGTGA
GCATCAGAATATTAAATCACTTTGTTTGAAACCTGTAGCGTCTGGACAAAGTCAATTTTCAGAGCTGTGTGAAGATGTAG
AAAGTATTCTAAATGCTTATAAGCATAAATTTACAGCAGCTGAAATAAATTTAATTTCATTCAATCAAGCAGTTGCTCCT
CATATAATTGCAGCAAAAACAAAAGTAGATATTTCTATTGAGAATCTTAAGCAATTTATCGAAGATAAGTACAATCAAGA
TTTAGATATTCTCTTTATTGAGGGTGCTGGAGGATTATTAACTCCCTACTCAGATCACACTACCCAGCTTGATCTAATAA
AGGCTCTGCAAATACCAGTACTACTAGTATCAGCTATAAAAGTTGGTTGCATAAACCATACCTTACTAACTATAAATGAG
TTAAATCGTCATAATATTAAATTAGCTGGTTGGATTGCTAATTGTAATGACTCAAATATCAAATACATTGATGAACAAAT
AAATACTATAGAAGAGCTATCAGGATATAAGTGTAGTGCAAAAATTTCTCGAAATGCTGATTATTTAGATTTTATAGATT
TATCCAAGATACTAATATCGCCAGAAGAAAATGAATAA

Upstream 100 bases:

>100_bases
CAAACAAAATTTCTCGAGTATTAGAAAGCTTTGTTTAAGCTCTAATCATATAAACCTCAGTTACCATGTTGGTCTATTTA
TTTGTTTTAAGGAATAAGTT

Downstream 100 bases:

>100_bases
TTCCCTTCGCTATTCTTAATAAGAATTTCGCCATTATTAGCTAAGTCAACATAGCTACAGTTATTATAAGTTTTATCTGC
TAGAGCAAATGAAAATACTT

Product: dethiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKKFFIIGTDTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAYKHKFTAAEINLISFNQAVAP
HIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLLTPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINE
LNRHNIKLAGWIANCNDSNIKYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILISPEENE

Sequences:

>Translated_225_residues
MKKFFIIGTDTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAYKHKFTAAEINLISFNQAVAP
HIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLLTPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINE
LNRHNIKLAGWIANCNDSNIKYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILISPEENE
>Mature_225_residues
MKKFFIIGTDTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAYKHKFTAAEINLISFNQAVAP
HIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLLTPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINE
LNRHNIKLAGWIANCNDSNIKYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILISPEENE

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=194, Percent_Identity=33.5051546391753, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI87081944, Length=214, Percent_Identity=28.0373831775701, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25189; Mature: 25189

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFFIIGTDTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAY
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KHKFTAAEINLISFNQAVAPHIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLL
HHCCCCEEEEEEECCCCCCCCEEEECEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCC
TPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINELNRHNIKLAGWIANCNDSNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCCCC
KYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILISPEENE
CHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHEECCCCCC
>Mature Secondary Structure
MKKFFIIGTDTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAY
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KHKFTAAEINLISFNQAVAPHIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLL
HHCCCCEEEEEEECCCCCCCCEEEECEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCC
TPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINELNRHNIKLAGWIANCNDSNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCCCC
KYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILISPEENE
CHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA