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The map label for this gene is dut

Identifier: 134302591

GI number: 134302591

Start: 1658361

End: 1658807

Strand: Reverse

Name: dut

Synonym: FTW_1763

Alternate gene names: 134302591

Gene position: 1658807-1658361 (Counterclockwise)

Preceding gene: 134302592

Following gene: 134302590

Centisome position: 87.38

GC content: 37.14

Gene sequence:

>447_bases
ATGAAGGTAGAGCTAAAAATTTTAAATAAAGAACTTATAAAAGAGTTACCCGGTTATGCGACTGAAGGTTCAGCAGCTAT
TGACTTAAGAGCATGTATCTCTGAGAGTATTTATCTTAAATCGGGTGAATGTAAACTTGTTGCAACTGGTATAGCTATTA
ATATTGCTAATCCAAATTATGCAGCAATGATTTTACCAAGATCTGGTTTGGGACATAAAAAAGGTTTGGTGCTAGGAAAT
GGTACGGGGCTTATAGATTCTGATTATCAAGGTGAGCTTATGGTTTCTTGTTTTAATCGCTCACAAGAGACTATTGAAAT
AGAACCGCTAATGAGATTCGCTCAGCTTGTAATTGTTCCTGTGGTACAAGCAAATTTTGAGATTGTTGAAGATTTTTCAC
AGCAAAGTGTCCGAGCTACTGGCGGCTTTGGACATACAGGGGTTTGA

Upstream 100 bases:

>100_bases
TGGTGGAGTGCTTGATAGGTTGGATGGATATATGCCAACTTTACCAATATTTGTGCTGCTTGGTTATTTAGCGGGAGTTT
TTGTTTTTTAGGGGGAAGAT

Downstream 100 bases:

>100_bases
TTTATGTTTTTTAATATCCCTAATATTCTGACTTTTGGTCGTTTGATATTAATTCCTTTTATAGTTATATGTTATTATTT
TGAGTTTCCACACCATCATG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKVELKILNKELIKELPGYATEGSAAIDLRACISESIYLKSGECKLVATGIAINIANPNYAAMILPRSGLGHKKGLVLGN
GTGLIDSDYQGELMVSCFNRSQETIEIEPLMRFAQLVIVPVVQANFEIVEDFSQQSVRATGGFGHTGV

Sequences:

>Translated_148_residues
MKVELKILNKELIKELPGYATEGSAAIDLRACISESIYLKSGECKLVATGIAINIANPNYAAMILPRSGLGHKKGLVLGN
GTGLIDSDYQGELMVSCFNRSQETIEIEPLMRFAQLVIVPVVQANFEIVEDFSQQSVRATGGFGHTGV
>Mature_148_residues
MKVELKILNKELIKELPGYATEGSAAIDLRACISESIYLKSGECKLVATGIAINIANPNYAAMILPRSGLGHKKGLVLGN
GTGLIDSDYQGELMVSCFNRSQETIEIEPLMRFAQLVIVPVVQANFEIVEDFSQQSVRATGGFGHTGV

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=57.4324324324324, Blast_Score=179, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI71988561, Length=134, Percent_Identity=34.3283582089552, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319729, Length=143, Percent_Identity=34.2657342657343, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_FRAT1 (Q14JC6)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666500.1
- ProteinModelPortal:   Q14JC6
- SMR:   Q14JC6
- STRING:   Q14JC6
- GeneID:   4199666
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0319
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q14JC6
- ProtClustDB:   PRK00601
- BioCyc:   FTUL393115:FTF0319-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15943; Mature: 15943

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVELKILNKELIKELPGYATEGSAAIDLRACISESIYLKSGECKLVATGIAINIANPNY
CEEEEEECCHHHHHHCCCCCCCCCCEEEHHHHHCCCEEEECCCEEEEEEEEEEEECCCCE
AAMILPRSGLGHKKGLVLGNGTGLIDSDYQGELMVSCFNRSQETIEIEPLMRFAQLVIVP
EEEEEECCCCCCCCCEEEECCCCEECCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHH
VVQANFEIVEDFSQQSVRATGGFGHTGV
HHHCCHHHHHHHHHHCEEECCCCCCCCC
>Mature Secondary Structure
MKVELKILNKELIKELPGYATEGSAAIDLRACISESIYLKSGECKLVATGIAINIANPNY
CEEEEEECCHHHHHHCCCCCCCCCCEEEHHHHHCCCEEEECCCEEEEEEEEEEEECCCCE
AAMILPRSGLGHKKGLVLGNGTGLIDSDYQGELMVSCFNRSQETIEIEPLMRFAQLVIVP
EEEEEECCCCCCCCCEEEECCCCEECCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHH
VVQANFEIVEDFSQQSVRATGGFGHTGV
HHHCCHHHHHHHHHHCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA