Click here to switch to the map view.

The map label for this gene is def1 [H]

Identifier: 134302514

GI number: 134302514

Start: 1583810

End: 1584442

Strand: Reverse

Name: def1 [H]

Synonym: FTW_1671

Alternate gene names: 134302514

Gene position: 1584442-1583810 (Counterclockwise)

Preceding gene: 134302515

Following gene: 134302513

Centisome position: 83.46

GC content: 31.44

Gene sequence:

>633_bases
ATGGTTGTAAATATCAAAATGCAACAAATGAAATCCCAATTTATACAGTATAACGACTCAAACAATAAGGTCTTGTACCA
AAAATGTAAACCTGTAGCAGATATTCAAAATGCTGAAATACAAAATATTATTACAGAAATGCATGAAAAAATGCAAGGTA
ATGGTATAGGATTGGCTGCTAATCAAATTGGTTATCCTTACCAAATATTTATGATAGAGTTTGATAGCTCAAATGCAAGA
TATCCATTTAGCTTTGATAGTGTACCATATCAGGTGTTTATAAATCCTAAAATTACAAAAGCCTCAAAACAAAGAGTAAG
TTTTTGGCATGGATGTTTGAGTGCCTTAGGTGAGAAAAGAGGCAAGCTAGCTACTTATAAAGAGATTGAATATGAGGCAT
ATAACCAACACGGTGAGAAAATTACAGGTAAACTTGACTCTATAGCAGCTATAATATTTCAGCATGAATTTAATCATCTA
CTTGGCTCTGTCTATGTTGATTTTGATACTGAGTATATCGATAACGAAGAACTACAAGCTAAATTTGCTAGTGGTGAACT
TAAACCTTACCAAGAATGTGGTGAAGAAGTTCCACTATTATTAGAAAAATACCAAATAGGTAAAAATATATAA

Upstream 100 bases:

>100_bases
AGTTAGTTTGAGTGGGGTTTAATAATTTTGAATACTACTGCTGGAGTCGCAGTATTGATCCAGTAAAACTTTGCTATAAT
CTTTAACATACAAGATTATG

Downstream 100 bases:

>100_bases
GTTTTTTTAGTAATATCAAAACTAATAGTTGACTTATGAATTAAGAAGAGTATTATAGCTTGCATTGATGATGATAGCAG
TCTGGAACCACCTGATCCCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAANQIGYPYQIFMIEFDSSNAR
YPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKRGKLATYKEIEYEAYNQHGEKITGKLDSIAAIIFQHEFNHL
LGSVYVDFDTEYIDNEELQAKFASGELKPYQECGEEVPLLLEKYQIGKNI

Sequences:

>Translated_210_residues
MVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAANQIGYPYQIFMIEFDSSNAR
YPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKRGKLATYKEIEYEAYNQHGEKITGKLDSIAAIIFQHEFNHL
LGSVYVDFDTEYIDNEELQAKFASGELKPYQECGEEVPLLLEKYQIGKNI
>Mature_210_residues
MVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAANQIGYPYQIFMIEFDSSNAR
YPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKRGKLATYKEIEYEAYNQHGEKITGKLDSIAAIIFQHEFNHL
LGSVYVDFDTEYIDNEELQAKFASGELKPYQECGEEVPLLLEKYQIGKNI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=31.5068493150685, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 24145; Mature: 24145

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAA
CEEEEHHHHHHHHHHEECCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEH
NQIGYPYQIFMIEFDSSNARYPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKR
HCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
GKLATYKEIEYEAYNQHGEKITGKLDSIAAIIFQHEFNHLLGSVYVDFDTEYIDNEELQA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHH
KFASGELKPYQECGEEVPLLLEKYQIGKNI
HHCCCCCCHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVVNIKMQQMKSQFIQYNDSNNKVLYQKCKPVADIQNAEIQNIITEMHEKMQGNGIGLAA
CEEEEHHHHHHHHHHEECCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEH
NQIGYPYQIFMIEFDSSNARYPFSFDSVPYQVFINPKITKASKQRVSFWHGCLSALGEKR
HCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
GKLATYKEIEYEAYNQHGEKITGKLDSIAAIIFQHEFNHLLGSVYVDFDTEYIDNEELQA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHH
KFASGELKPYQECGEEVPLLLEKYQIGKNI
HHCCCCCCHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]