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The map label for this gene is murD [H]

Identifier: 134302471

GI number: 134302471

Start: 1523013

End: 1524263

Strand: Reverse

Name: murD [H]

Synonym: FTW_1619

Alternate gene names: 134302471

Gene position: 1524263-1523013 (Counterclockwise)

Preceding gene: 134302472

Following gene: 134302470

Centisome position: 80.29

GC content: 29.74

Gene sequence:

>1251_bases
ATGTTTAGTTTTTATTTTAATGATAACAAGATAACCAAACTATTAATGGTTGGTTATGGTTCTACTGGTAAGTCTGTTTG
TGATTTTCTTGCGAACTTTATAGATATTACAGTTGATATATCGCAGAATGATGATGAGTTTGTTAATTATGATTTAAACA
GTTATGACTTAATTACAGTCAGCCCTGGGATTCCTCTTAATAAATCTCCATATAGAGCCCTAACTAAATTTAAAGATAAA
ATAGTCAGTGATATTGATATATTCTATCAGTATATCAAGGATACTAAAGCTAAAACAATTGCTGTTACAGGATCAAACGG
TAAAAGTACAGTAGTAACAATGACTGATTTTGTCCTCAAGGATCTTGGTTATAAGAGTATTCTTGTTGGTAATATTGGTA
CCCCAGCTTTAAACAAAATAGGTGAGAAATTTGATTACTGTGTTGTTGAAGTATCAAGCTTTCAGATAAATTTATTTAAT
TGTGTTAGATTTGATCTAGGTTGTATTATTAATGTTTCACCAGATCATTTAGATAGATACCAAAATTTTGAGCAATACAA
GCAGTCAAAACTTAATTTGGCAAAATTCAGTAACGATTTTTTTGTTTATGATGTGCATAATGGTATTAAGTATGCTGGCG
AATATCAAATAATAAGAGGCGCTATCTACCGTAATTCAACAAAGCTATTAGATATTGTTGAGACTAAACTTTTTGGTGAA
CATAATTTAGAAAATATTATTGTTGTGCTAAATATCCTTGATAGGTTAGGTTTAGATATTAATCAAGCAATAGCCTCTAT
TAAAAAATTTAAAGGCTTAGAACATCGCTGTAAGATTGTAAAGAAAGTAAATGGTACAACTTATATAAATGATTCAAAAG
GTACTAATGTTGGAGCTACAATAGCTGCTCTTAATAGTATAACAAATTCTAAAAATATCATATTATTATTGGGTGGTGTA
GCAAAGGGCGGTGATTTTAGTTTGATGATAAAATCACTAGATAAATATGTTAAATATGTCTATATATATGGAGCTGATAA
AGAATATATTGAAAGCTATATCAAAGGCTACTGTAAATATCAATTATGTAATAATATGAAACAAGCTTTCGAATTGGCTA
GTCAAAAAGCCAATTCCAATGAGATAGTGTTATTATCTCCAGCATGTGCAAGCTTTGATGAATTTAGTGGTTATGCACAG
CGCGGTGAAGTTTTTCAAAATCTTGTTGCTCAGCTAGAGCAAAAAAGTTAG

Upstream 100 bases:

>100_bases
TTGAATTGAAAGGCTGGCCAGAAACAAAAGTTGTGATACGATTTTGGATAATTTCTTTGATACTTTTCTTAATTGGTTTG
GCAGCTATCAAGGTTAGATA

Downstream 100 bases:

>100_bases
GAAGTATAAGTGTTATATAGATTAAAACTATTGTTGAGTGGCAAAAACTCAAAAAAAGAACGTGTAAGAGCCAAATTAGA
GATTGATATTTCAATTGTCT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFSFYFNDNKITKLLMVGYGSTGKSVCDFLANFIDITVDISQNDDEFVNYDLNSYDLITVSPGIPLNKSPYRALTKFKDK
IVSDIDIFYQYIKDTKAKTIAVTGSNGKSTVVTMTDFVLKDLGYKSILVGNIGTPALNKIGEKFDYCVVEVSSFQINLFN
CVRFDLGCIINVSPDHLDRYQNFEQYKQSKLNLAKFSNDFFVYDVHNGIKYAGEYQIIRGAIYRNSTKLLDIVETKLFGE
HNLENIIVVLNILDRLGLDINQAIASIKKFKGLEHRCKIVKKVNGTTYINDSKGTNVGATIAALNSITNSKNIILLLGGV
AKGGDFSLMIKSLDKYVKYVYIYGADKEYIESYIKGYCKYQLCNNMKQAFELASQKANSNEIVLLSPACASFDEFSGYAQ
RGEVFQNLVAQLEQKS

Sequences:

>Translated_416_residues
MFSFYFNDNKITKLLMVGYGSTGKSVCDFLANFIDITVDISQNDDEFVNYDLNSYDLITVSPGIPLNKSPYRALTKFKDK
IVSDIDIFYQYIKDTKAKTIAVTGSNGKSTVVTMTDFVLKDLGYKSILVGNIGTPALNKIGEKFDYCVVEVSSFQINLFN
CVRFDLGCIINVSPDHLDRYQNFEQYKQSKLNLAKFSNDFFVYDVHNGIKYAGEYQIIRGAIYRNSTKLLDIVETKLFGE
HNLENIIVVLNILDRLGLDINQAIASIKKFKGLEHRCKIVKKVNGTTYINDSKGTNVGATIAALNSITNSKNIILLLGGV
AKGGDFSLMIKSLDKYVKYVYIYGADKEYIESYIKGYCKYQLCNNMKQAFELASQKANSNEIVLLSPACASFDEFSGYAQ
RGEVFQNLVAQLEQKS
>Mature_416_residues
MFSFYFNDNKITKLLMVGYGSTGKSVCDFLANFIDITVDISQNDDEFVNYDLNSYDLITVSPGIPLNKSPYRALTKFKDK
IVSDIDIFYQYIKDTKAKTIAVTGSNGKSTVVTMTDFVLKDLGYKSILVGNIGTPALNKIGEKFDYCVVEVSSFQINLFN
CVRFDLGCIINVSPDHLDRYQNFEQYKQSKLNLAKFSNDFFVYDVHNGIKYAGEYQIIRGAIYRNSTKLLDIVETKLFGE
HNLENIIVVLNILDRLGLDINQAIASIKKFKGLEHRCKIVKKVNGTTYINDSKGTNVGATIAALNSITNSKNIILLLGGV
AKGGDFSLMIKSLDKYVKYVYIYGADKEYIESYIKGYCKYQLCNNMKQAFELASQKANSNEIVLLSPACASFDEFSGYAQ
RGEVFQNLVAQLEQKS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=434, Percent_Identity=32.0276497695853, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46735; Mature: 46735

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFYFNDNKITKLLMVGYGSTGKSVCDFLANFIDITVDISQNDDEFVNYDLNSYDLITV
CEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCEEEECCCCEEEEEE
SPGIPLNKSPYRALTKFKDKIVSDIDIFYQYIKDTKAKTIAVTGSNGKSTVVTMTDFVLK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHH
DLGYKSILVGNIGTPALNKIGEKFDYCVVEVSSFQINLFNCVRFDLGCIINVSPDHLDRY
HCCCCEEEEECCCCHHHHHHHHHHCEEEEEEECEEEEEEHEEEECCCEEEECCHHHHHHH
QNFEQYKQSKLNLAKFSNDFFVYDVHNGIKYAGEYQIIRGAIYRNSTKLLDIVETKLFGE
HHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHCCC
HNLENIIVVLNILDRLGLDINQAIASIKKFKGLEHRCKIVKKVNGTTYINDSKGTNVGAT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCHHH
IAALNSITNSKNIILLLGGVAKGGDFSLMIKSLDKYVKYVYIYGADKEYIESYIKGYCKY
HHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHH
QLCNNMKQAFELASQKANSNEIVLLSPACASFDEFSGYAQRGEVFQNLVAQLEQKS
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSFYFNDNKITKLLMVGYGSTGKSVCDFLANFIDITVDISQNDDEFVNYDLNSYDLITV
CEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCEEEECCCCEEEEEE
SPGIPLNKSPYRALTKFKDKIVSDIDIFYQYIKDTKAKTIAVTGSNGKSTVVTMTDFVLK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHH
DLGYKSILVGNIGTPALNKIGEKFDYCVVEVSSFQINLFNCVRFDLGCIINVSPDHLDRY
HCCCCEEEEECCCCHHHHHHHHHHCEEEEEEECEEEEEEHEEEECCCEEEECCHHHHHHH
QNFEQYKQSKLNLAKFSNDFFVYDVHNGIKYAGEYQIIRGAIYRNSTKLLDIVETKLFGE
HHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHCCC
HNLENIIVVLNILDRLGLDINQAIASIKKFKGLEHRCKIVKKVNGTTYINDSKGTNVGAT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCHHH
IAALNSITNSKNIILLLGGVAKGGDFSLMIKSLDKYVKYVYIYGADKEYIESYIKGYCKY
HHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHH
QLCNNMKQAFELASQKANSNEIVLLSPACASFDEFSGYAQRGEVFQNLVAQLEQKS
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA