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The map label for this gene is ansB [H]

Identifier: 134302458

GI number: 134302458

Start: 1511498

End: 1512559

Strand: Reverse

Name: ansB [H]

Synonym: FTW_1606

Alternate gene names: 134302458

Gene position: 1512559-1511498 (Counterclockwise)

Preceding gene: 134302459

Following gene: 134302457

Centisome position: 79.67

GC content: 30.98

Gene sequence:

>1062_bases
ATGCAATCGATATTTAAAAAAACTATAATTGTTATAGTTATATTTCTATCATCATATTTAGTCGCTCTAGCAGCTTTACC
AAATGTATACATTGTTGCAACTGGTGGAACTATTGCAGGGGTTGCAAAATCATCAACAGCAGCTGTGTATAAAGCTGGAA
GTTTATCAATTAATGATATTTTAGAAAAAGAACCAGCTTTTGGTAAATTGGCGAATATAAAATCTGAACAATTTTATAAT
ATAGATAGTGTAGATATGACAATGGCAATGCGAATTAAGCTTGCTGAGTATGTACAAAAGCTAATAGATGATCCAAATGT
TAATGCTGTAATTATTACTCATGGTACTGATAGTATGGTACAAACAGCATTTTTCCTTAATCAAGTTTTAGATGTAAAAA
AACCTGTCATTTTAGTAGGCGCTATGAGAGCTTTTACATCATTAAGTAGTGATGCGCTTATGAATTTATATGATGCTTTA
GTTACTGCTGTAAACAAACAGTCAATTGGCAAGGGCGTCTTAGTTGTGATGAATGAAGAAATTTTAACTGCAAATGATGT
TGCTAAGACTAATACAACAAATGTTGATGCGTTTAAAGCTCCAAATTATGGTAAATTAGGTACAGTAATAATTAATGATG
TTGATTATTATCAAAGTCCTAGGGTTGTTGGTAATATATTGAGTGTTGATGATTTACAAAAAATAACTGCCTTACCTAAA
GTTGAGGTAATTTATGAATCAGCTGATATATCACCAGAGTTTTTAGATAGTGTCTTGACGATCAAAGGCTTAGAGGGTAT
AGTTCTTGCAGGTCTTGGTGATGGCAATATTCCATCTAATCAAGGAGATTTTCTTAAAAAAGCACGAGCTAAGTGTATTG
TAGTAGTTCGCTCTAGTTATGTCGGTTCTAAAGTTACTCATAACTATAATAATTTGGATGACAAATTTGATCTTGTATCA
TCAGCAACACTTTCACCAGAAAAAGCGAGAATATTTTTACAGTTATGTTTAATAAAAACTCATGATATTAAAAAAATTCA
AAAACTATTTGATAGATTTTAG

Upstream 100 bases:

>100_bases
AATTCGCCCTGTTAAATTTTTTATTTTTTAGTAGTTTTTTATAATCTTGAGGTAGAATTATGCTTTAATCAAGGTCTGAT
TTTAGTTAATAGTAAATATT

Downstream 100 bases:

>100_bases
TGGCTGAATTGATACAGCTTATTAGGCATCAGATAATATATTTGTCATCTTAAACTAGTTTTAGGATTTCAATAAAATCA
ATACTTACAGTAAAATGCTG

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MQSIFKKTIIVIVIFLSSYLVALAALPNVYIVATGGTIAGVAKSSTAAVYKAGSLSINDILEKEPAFGKLANIKSEQFYN
IDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMVQTAFFLNQVLDVKKPVILVGAMRAFTSLSSDALMNLYDAL
VTAVNKQSIGKGVLVVMNEEILTANDVAKTNTTNVDAFKAPNYGKLGTVIINDVDYYQSPRVVGNILSVDDLQKITALPK
VEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNIPSNQGDFLKKARAKCIVVVRSSYVGSKVTHNYNNLDDKFDLVS
SATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF

Sequences:

>Translated_353_residues
MQSIFKKTIIVIVIFLSSYLVALAALPNVYIVATGGTIAGVAKSSTAAVYKAGSLSINDILEKEPAFGKLANIKSEQFYN
IDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMVQTAFFLNQVLDVKKPVILVGAMRAFTSLSSDALMNLYDAL
VTAVNKQSIGKGVLVVMNEEILTANDVAKTNTTNVDAFKAPNYGKLGTVIINDVDYYQSPRVVGNILSVDDLQKITALPK
VEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNIPSNQGDFLKKARAKCIVVVRSSYVGSKVTHNYNNLDDKFDLVS
SATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF
>Mature_353_residues
MQSIFKKTIIVIVIFLSSYLVALAALPNVYIVATGGTIAGVAKSSTAAVYKAGSLSINDILEKEPAFGKLANIKSEQFYN
IDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMVQTAFFLNQVLDVKKPVILVGAMRAFTSLSSDALMNLYDAL
VTAVNKQSIGKGVLVVMNEEILTANDVAKTNTTNVDAFKAPNYGKLGTVIINDVDYYQSPRVVGNILSVDDLQKITALPK
VEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNIPSNQGDFLKKARAKCIVVVRSSYVGSKVTHNYNNLDDKFDLVS
SATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=351, Percent_Identity=40.7407407407407, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1788065, Length=278, Percent_Identity=29.4964028776978, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=318, Percent_Identity=27.0440251572327, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17558278, Length=318, Percent_Identity=27.0440251572327, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193207372, Length=318, Percent_Identity=27.0440251572327, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=318, Percent_Identity=27.0440251572327, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320527, Length=333, Percent_Identity=35.4354354354354, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323189, Length=334, Percent_Identity=31.7365269461078, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323187, Length=334, Percent_Identity=31.7365269461078, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323186, Length=334, Percent_Identity=31.7365269461078, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323184, Length=334, Percent_Identity=31.7365269461078, Blast_Score=171, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38396; Mature: 38396

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIFKKTIIVIVIFLSSYLVALAALPNVYIVATGGTIAGVAKSSTAAVYKAGSLSINDI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEEECCCCCHHHH
LEKEPAFGKLANIKSEQFYNIDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMV
HHCCCCCCHHHCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
QTAFFLNQVLDVKKPVILVGAMRAFTSLSSDALMNLYDALVTAVNKQSIGKGVLVVMNEE
HHHHHHHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
ILTANDVAKTNTTNVDAFKAPNYGKLGTVIINDVDYYQSPRVVGNILSVDDLQKITALPK
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCHHHHHHCCHHHHHHHHCCCC
VEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNIPSNQGDFLKKARAKCIVVVRSSY
EEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEEECCC
VGSKVTHNYNNLDDKFDLVSSATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF
CCCHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQSIFKKTIIVIVIFLSSYLVALAALPNVYIVATGGTIAGVAKSSTAAVYKAGSLSINDI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEEECCCCCHHHH
LEKEPAFGKLANIKSEQFYNIDSVDMTMAMRIKLAEYVQKLIDDPNVNAVIITHGTDSMV
HHCCCCCCHHHCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
QTAFFLNQVLDVKKPVILVGAMRAFTSLSSDALMNLYDALVTAVNKQSIGKGVLVVMNEE
HHHHHHHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
ILTANDVAKTNTTNVDAFKAPNYGKLGTVIINDVDYYQSPRVVGNILSVDDLQKITALPK
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCHHHHHHCCHHHHHHHHCCCC
VEVIYESADISPEFLDSVLTIKGLEGIVLAGLGDGNIPSNQGDFLKKARAKCIVVVRSSY
EEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEEECCC
VGSKVTHNYNNLDDKFDLVSSATLSPEKARIFLQLCLIKTHDIKKIQKLFDRF
CCCHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]