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The map label for this gene is potF [H]

Identifier: 134302441

GI number: 134302441

Start: 1491446

End: 1492588

Strand: Reverse

Name: potF [H]

Synonym: FTW_1589

Alternate gene names: 134302441

Gene position: 1492588-1491446 (Counterclockwise)

Preceding gene: 134302447

Following gene: 134302438

Centisome position: 78.62

GC content: 29.92

Gene sequence:

>1143_bases
TTGTTTAATCTGGCATATTCAAAAGATGAGTCATATATTTGTGATAATAAAATTCTTAAAAGTCCTATCACTGCTACACA
AACTAGAACTCAATTAAATTTTACTAACTGGGCAGACTATATATCTCCAGATATAGTACCATGTTTTTTAGACTTATCTA
ATACTAGAGTCAAGTATATCTATACTTCTGATGATAATATGACTCGTGCGAAAATTATGACAGGATCATCTGGTTTTGAT
TTAATTGAGCAAGGTGCATTATATTTAAATAGTGAAATCGCATCTAATGCTCTTGTTAAATTAGATAAATCAAAATTACC
AAATCTAAAATATCGTAATAAAGTAATTTATGATAAAGTTTCACAGATAAATGATCCAGGTAATAATTATGCTGTGGTAT
ATAGTTATGGAACTACAGGTTTAGTTTATAACAAACAAGAAATAGAGCAAAGACTAGGTAAAGGTGTGGTTCCTAATAGC
TGGAAATATGTTTTTGATAAAAAATACCTCAAACAAATAGCTCCATGTGGAGTTTCGTTACTTGATGAACCTGAGCAAAT
ATTTGGTAATTATTTTTTCTATCACGGAATTGATCCAAATACCAATAGCAAGGCTGAATATGAAAAAGCAGCTTTAGATA
TTATTAAAAATGTCCGCCCATATATTAAATATTTTGATAGTAATAAATATCAAAATGATTTTACCGCGGGTAATCTTTGT
TTAGTTATGGGTTATTCTGGTGATGTAGTTCGATCTGTTGAAAGAGCTAAGTCAGTAAATCCGGATGTCACACTTGCATA
TGTTATTCCTAAAGAGGGTACTAATATTTGGTTTGATATGCTAATGATACCAAAAGGTGCTAAAGATTTAGATAAAGCTT
ATGCTTTGATGAACTATATTATAGATCCATATGTATCAGCACAAAACAGTAATTATTTGTATCAGCCAAATGCTGTTACG
CAAAACAAAAAATATCTAGATGATATCTTTGATGATACTAATATCAGACCTACTGATGAGATGATTAAGAAAATGTACGT
TCTTAATATCCATGATGCCAAAATGCAGAGTTTTATTAGTAGAATGTGGATGAATGTTAAGTACGGTATAGAATTTACAC
CAAAGTATTATAAGCCGCAATAA

Upstream 100 bases:

>100_bases
ATACCACGCTTTAGTATATACTTAAAGGAATTTTATAACAACTAGAGCGTGTGTTACTTTTATGAAACGAAAGGTTTTTA
TTATTGGCCTGCTAGTTAGT

Downstream 100 bases:

>100_bases
AATAAGTGGCTTATTGTAGATTAGCCGTACAAACTAAACTTAACTTCTAAAATCAAAATCTCAAAATTTATGAGAGTAAA
TTCAAACTATAATAAAAATC

Product: hypothetical protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPDIVPCFLDLSNTRVKYIYTSDDNMTRAKIMTGSSGFD
LIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLVYNKQEIEQRLGKGVVPNS
WKYVFDKKYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDSNKYQNDFTAGNLC
LVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYIIDPYVSAQNSNYLYQPNAVT
QNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ

Sequences:

>Translated_380_residues
MFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPDIVPCFLDLSNTRVKYIYTSDDNMTRAKIMTGSSGFD
LIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLVYNKQEIEQRLGKGVVPNS
WKYVFDKKYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDSNKYQNDFTAGNLC
LVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYIIDPYVSAQNSNYLYQPNAVT
QNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ
>Mature_380_residues
MFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPDIVPCFLDLSNTRVKYIYTSDDNMTRAKIMTGSSGFD
LIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKVSQINDPGNNYAVVYSYGTTGLVYNKQEIEQRLGKGVVPNS
WKYVFDKKYLKQIAPCGVSLLDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDSNKYQNDFTAGNLC
LVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYIIDPYVSAQNSNYLYQPNAVT
QNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFISRMWMNVKYGIEFTPKYYKPQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=347, Percent_Identity=33.1412103746398, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1787367, Length=315, Percent_Identity=28.2539682539683, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 43753; Mature: 43753

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPDIVPCFLDLSNTRVKYI
CCCCCCCCCCCEECCCHHHCCCCCCCCCEEEECCCCCHHHCCCCCEEEEEECCCCEEEEE
YTSDDNMTRAKIMTGSSGFDLIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKV
EECCCCCEEEEEEECCCCCHHHHCCCEEECHHHHCCHHEEECHHHCCCCCCCCHHHHHHH
SQINDPGNNYAVVYSYGTTGLVYNKQEIEQRLGKGVVPNSWKYVFDKKYLKQIAPCGVSL
HHCCCCCCCEEEEEEECCCCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHH
LDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDSNKYQNDFTAGNLC
CCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
LVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYI
EEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHH
IDPYVSAQNSNYLYQPNAVTQNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFIS
HCCCEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHEEEEEECHHHHHHHHH
RMWMNVKYGIEFTPKYYKPQ
HHHCEEEECEEECCCCCCCC
>Mature Secondary Structure
MFNLAYSKDESYICDNKILKSPITATQTRTQLNFTNWADYISPDIVPCFLDLSNTRVKYI
CCCCCCCCCCCEECCCHHHCCCCCCCCCEEEECCCCCHHHCCCCCEEEEEECCCCEEEEE
YTSDDNMTRAKIMTGSSGFDLIEQGALYLNSEIASNALVKLDKSKLPNLKYRNKVIYDKV
EECCCCCEEEEEEECCCCCHHHHCCCEEECHHHHCCHHEEECHHHCCCCCCCCHHHHHHH
SQINDPGNNYAVVYSYGTTGLVYNKQEIEQRLGKGVVPNSWKYVFDKKYLKQIAPCGVSL
HHCCCCCCCEEEEEEECCCCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHH
LDEPEQIFGNYFFYHGIDPNTNSKAEYEKAALDIIKNVRPYIKYFDSNKYQNDFTAGNLC
CCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
LVMGYSGDVVRSVERAKSVNPDVTLAYVIPKEGTNIWFDMLMIPKGAKDLDKAYALMNYI
EEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHH
IDPYVSAQNSNYLYQPNAVTQNKKYLDDIFDDTNIRPTDEMIKKMYVLNIHDAKMQSFIS
HCCCEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHEEEEEECHHHHHHHHH
RMWMNVKYGIEFTPKYYKPQ
HHHCEEEECEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]