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The map label for this gene is dmlR [H]

Identifier: 134302431

GI number: 134302431

Start: 1479544

End: 1480470

Strand: Direct

Name: dmlR [H]

Synonym: FTW_1577

Alternate gene names: 134302431

Gene position: 1479544-1480470 (Clockwise)

Preceding gene: 134302430

Following gene: 134302432

Centisome position: 77.93

GC content: 28.91

Gene sequence:

>927_bases
ATGAAAATGTTATATGAGCTAAAACTATTTGTAGCTGTAATTAACTACGGTAACTTATCTGCTGCTGGACGAGAATTTTA
TTTATCTCCAGCTAGTATTAGTAATAAAATTAATGCCCTCGAAGAGTATTATCAAACCAAGCTTTTAATAAGAATTACAA
GAAAGATTGAACCTACTAAAGCAGGAGAAAAGCTTTATACTACTGCTATTAAATTATTAGAACAACTAGAGAACCTTCAA
GAAGATATTCAAAATAATGAAAACTCCGCAGAAGGTGTTATTAAGATAACGATTCCTTTTGACTTAGGTAAGCAGATAAT
CCTACCAATTTTAGATGAATTCAAAGAAAAAAATCCCAACATACAATACGATATAAATCTCTCTGATGATGTAATGTCTT
ATAACCAGTTTCCTTTTGATATAGCAATTAGATATGGCAATTTACCAGATAGTAACTTTATTGCAAAAAAGCTTGTCGAT
AACTATAGAATGTTATGTGCCAGCCCTGAATATCTAAAAAAGATGGACATATCTAACACTGATGATATAAACATATTACA
AAATTGTGATTTCATAACATTAAAAATAAACTCTAACACAATCAAAAAATGGTATTTAATTGATCAAGAGAATAATAAAC
ATGAAGTTAACATAAAGCCATCTTACATAGTTAATAATGGTTATATAAGCCGACAAATGTGTCTTAATGGAAATGGAATA
AGTGAAAAATCATATTGGGATGTCAAGCAAGATCTAAAATCAGGTAAATTAGTTCAGGTTTTACCAAATTATAGAGTAAC
TCTAAACAAAAAGGATAAGCCTGATAATATGATAAGCCTTCTATATCCGTCCGATCAGTTTCAACCTTACAGAATAAGAA
TGTTAAGTAAATTTATAGTAGATAGTTTCTCAAAATTCAAAAGTTAG

Upstream 100 bases:

>100_bases
ATAAATTTAAACTTTCATTGCTTGACACCAATTATATACTTTTAACTTTTTCAATGATAATATACAAAAATATTAAATGA
CTTTTTATAAAAACTAAAAA

Downstream 100 bases:

>100_bases
AAACATTGCTTAAATTTAGATATAATCTTGACTAAAAAAGTTGATTTCTTATACGATGATAAAAAATATATTTTTTGACT
TAGATGGTACGCTTGTAAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRITRKIEPTKAGEKLYTTAIKLLEQLENLQ
EDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPNIQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVD
NYRMLCASPEYLKKMDISNTDDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIVDSFSKFKS

Sequences:

>Translated_308_residues
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRITRKIEPTKAGEKLYTTAIKLLEQLENLQ
EDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPNIQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVD
NYRMLCASPEYLKKMDISNTDDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIVDSFSKFKS
>Mature_308_residues
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRITRKIEPTKAGEKLYTTAIKLLEQLENLQ
EDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPNIQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVD
NYRMLCASPEYLKKMDISNTDDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIVDSFSKFKS

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=306, Percent_Identity=28.7581699346405, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789440, Length=296, Percent_Identity=31.0810810810811, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=27.1186440677966, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI145693193, Length=315, Percent_Identity=26.984126984127, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787589, Length=268, Percent_Identity=25.3731343283582, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789639, Length=267, Percent_Identity=22.8464419475655, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=25, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787128, Length=301, Percent_Identity=21.5946843853821, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=169, Percent_Identity=24.8520710059172, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786448, Length=258, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788297, Length=198, Percent_Identity=23.2323232323232, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=263, Percent_Identity=20.532319391635, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35770; Mature: 35770

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRITRKIEPTK
CCHHHHHEEEHHEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
AGEKLYTTAIKLLEQLENLQEDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
IQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVDNYRMLCASPEYLKKMDISNT
EEEEECCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCHHHHHHCCCCCC
DDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
CCCEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCEEEECCEEEEEEEECCCCC
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIV
CCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
DSFSKFKS
HHHHHHCC
>Mature Secondary Structure
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRITRKIEPTK
CCHHHHHEEEHHEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
AGEKLYTTAIKLLEQLENLQEDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
IQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVDNYRMLCASPEYLKKMDISNT
EEEEECCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCHHHHHHCCCCCC
DDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
CCCEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCEEEECCEEEEEEEECCCCC
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIV
CCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
DSFSKFKS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]