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The map label for this gene is miaE [H]

Identifier: 134302367

GI number: 134302367

Start: 1395061

End: 1395672

Strand: Reverse

Name: miaE [H]

Synonym: FTW_1491

Alternate gene names: 134302367

Gene position: 1395672-1395061 (Counterclockwise)

Preceding gene: 134302370

Following gene: 134302360

Centisome position: 73.52

GC content: 33.33

Gene sequence:

>612_bases
ATGATAAAACAACAATATGCAGATTTTGCAACTATCGAAAATTTTCTATTATGTGAAACTCCTAAGCAATGGATACACAA
GGCTTTAGCAAATGTTGAGCTGATGCTCATAGATCATGCTCATTGTGAAATGAAAGCAGCATCTTCTGCGATGACATACA
TCTACAAGCATCCAGATAAGTATGATTTAGTTACCAGAATGTCAAAGATAGCTAGAGAAGAATTGGTTCATTTTGAACAG
GTAATGCGTATCCTAAAAAAACGTGATATAGAATATAGGGCTATAACCGCTTCAAGATATGCTAGTCAATTAATCAAGGC
TGCTAGAACAGATAAAGAAGGGCGTTTTATAGATGCTTTGATAATTGGAGCTTATATTGAAGCGCGTTCTTGCGAAAGAT
TCGCAAAAATTGCACCGTTTTTAGATGAAGAATTACAAAAGTTTTACAATGGTCTCTTAGAATCTGAAAAAAGGCATTTT
ACAATATATCTTCACTTCGCAGAAAAATATTCATCAGTAGATATTGGAAAGCATATAGCAAGAATAGGTGAAGTAGAAAA
AGAACTTATACTATCTACTGATAGTGAGTTTCGTTTTCATAGTGGTATTTAA

Upstream 100 bases:

>100_bases
AAATATTTTTTATCTAAAAATAATTCTACTCTTATTGTATTTTAAATAGAATAAATCAACTATATTTTTTATAATTACCG
CAAAAAATATTTTCTAAAGC

Downstream 100 bases:

>100_bases
AACTTCAAAATTTTTTGTTATTAGCATTTCTAGTATTTATAATTCTATATTATTAGATAATTGGTAATTTCCACTTATAA
ATTATTGACATAATTCTCAC

Product: tRNA-(ms(2)io(6)a)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIKQQYADFATIENFLLCETPKQWIHKALANVELMLIDHAHCEMKAASSAMTYIYKHPDKYDLVTRMSKIAREELVHFEQ
VMRILKKRDIEYRAITASRYASQLIKAARTDKEGRFIDALIIGAYIEARSCERFAKIAPFLDEELQKFYNGLLESEKRHF
TIYLHFAEKYSSVDIGKHIARIGEVEKELILSTDSEFRFHSGI

Sequences:

>Translated_203_residues
MIKQQYADFATIENFLLCETPKQWIHKALANVELMLIDHAHCEMKAASSAMTYIYKHPDKYDLVTRMSKIAREELVHFEQ
VMRILKKRDIEYRAITASRYASQLIKAARTDKEGRFIDALIIGAYIEARSCERFAKIAPFLDEELQKFYNGLLESEKRHF
TIYLHFAEKYSSVDIGKHIARIGEVEKELILSTDSEFRFHSGI
>Mature_203_residues
MIKQQYADFATIENFLLCETPKQWIHKALANVELMLIDHAHCEMKAASSAMTYIYKHPDKYDLVTRMSKIAREELVHFEQ
VMRILKKRDIEYRAITASRYASQLIKAARTDKEGRFIDALIIGAYIEARSCERFAKIAPFLDEELQKFYNGLLESEKRHF
TIYLHFAEKYSSVDIGKHIARIGEVEKELILSTDSEFRFHSGI

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 23715; Mature: 23715

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQQYADFATIENFLLCETPKQWIHKALANVELMLIDHAHCEMKAASSAMTYIYKHPDK
CCCHHHHHHHHHHHHHEECCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHCCCCH
YDLVTRMSKIAREELVHFEQVMRILKKRDIEYRAITASRYASQLIKAARTDKEGRFIDAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IIGAYIEARSCERFAKIAPFLDEELQKFYNGLLESEKRHFTIYLHFAEKYSSVDIGKHIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHCCCCHHHHHHH
RIGEVEKELILSTDSEFRFHSGI
HHHHHHHHHHHCCCCCCEECCCC
>Mature Secondary Structure
MIKQQYADFATIENFLLCETPKQWIHKALANVELMLIDHAHCEMKAASSAMTYIYKHPDK
CCCHHHHHHHHHHHHHEECCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHCCCCH
YDLVTRMSKIAREELVHFEQVMRILKKRDIEYRAITASRYASQLIKAARTDKEGRFIDAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IIGAYIEARSCERFAKIAPFLDEELQKFYNGLLESEKRHFTIYLHFAEKYSSVDIGKHIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHCCCCHHHHHHH
RIGEVEKELILSTDSEFRFHSGI
HHHHHHHHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]