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The map label for this gene is rsmD [H]

Identifier: 134302365

GI number: 134302365

Start: 1393746

End: 1394324

Strand: Direct

Name: rsmD [H]

Synonym: FTW_1489

Alternate gene names: 134302365

Gene position: 1393746-1394324 (Clockwise)

Preceding gene: 134302364

Following gene: 134302366

Centisome position: 73.41

GC content: 29.36

Gene sequence:

>579_bases
GTGAAAACCAATTCTATCCGTGTGATTGCTGGTAAATATAAAAATCGTAGATTGAAGTTTCCAAACGCTAATGGTTTACG
ACCAACATCTGATCAACTAAAAGAAACGATTTTTAATTGGTTAAGTCCATTTATCCATGATAGTATTTGTATCGATGCTT
TTGCTGGTAGCGGTAGTTTAGGGATAGAAAGTATCTCACGTGGTGCTGCTAAAGCAATTTTTTATGAACTTAATTTTAAA
GCACTACTACAAATTAAAGAAAATCTTAAAACTCTCGCTATAGAAAATTTTGAAATATATAAAATAGATAGCATCAAAGC
TCTCGCTAATTTAAATGTCCCAAGTTCACGCTTAATTATCTTTTTAGATCCGCCATTTAATAAGAATATTGTCCCACTAG
CACTAAAGTCAATACTAGAAAATCGACATATCCTAGACCAAACATTAATATATATTGAGACTGAAAAAACAGCTGAATAT
AGTCTTGAAGGCTTTGATATTTTAAAAGAGAAAAATTCTACAAATATTTCAGCAAAATTAGTATCAAAAAATCATTTAAA
TTCTAAAAATAATCTCTAA

Upstream 100 bases:

>100_bases
TGAGAATATCTGTTGTGTCACTGCTGAAGAGATACATCAAGCTTTCTTAGCAGTCCAAACTAATAAACTCATAACAGTAA
TGGTCGGAGATATTGAATAA

Downstream 100 bases:

>100_bases
AATATAGCCAACTTAGCTTGTCGGAAATAACATATGTATCACGTCGGTATCACTGGACCTTTTATTTTTTCAGTTATGTT
TCTTTTAGGCATAATTGCTG

Product: putative methyltransferase family protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKTNSIRVIAGKYKNRRLKFPNANGLRPTSDQLKETIFNWLSPFIHDSICIDAFAGSGSLGIESISRGAAKAIFYELNFK
ALLQIKENLKTLAIENFEIYKIDSIKALANLNVPSSRLIIFLDPPFNKNIVPLALKSILENRHILDQTLIYIETEKTAEY
SLEGFDILKEKNSTNISAKLVSKNHLNSKNNL

Sequences:

>Translated_192_residues
MKTNSIRVIAGKYKNRRLKFPNANGLRPTSDQLKETIFNWLSPFIHDSICIDAFAGSGSLGIESISRGAAKAIFYELNFK
ALLQIKENLKTLAIENFEIYKIDSIKALANLNVPSSRLIIFLDPPFNKNIVPLALKSILENRHILDQTLIYIETEKTAEY
SLEGFDILKEKNSTNISAKLVSKNHLNSKNNL
>Mature_192_residues
MKTNSIRVIAGKYKNRRLKFPNANGLRPTSDQLKETIFNWLSPFIHDSICIDAFAGSGSLGIESISRGAAKAIFYELNFK
ALLQIKENLKTLAIENFEIYKIDSIKALANLNVPSSRLIIFLDPPFNKNIVPLALKSILENRHILDQTLIYIETEKTAEY
SLEGFDILKEKNSTNISAKLVSKNHLNSKNNL

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=153, Percent_Identity=39.2156862745098, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 21652; Mature: 21652

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNSIRVIAGKYKNRRLKFPNANGLRPTSDQLKETIFNWLSPFIHDSICIDAFAGSGSL
CCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
GIESISRGAAKAIFYELNFKALLQIKENLKTLAIENFEIYKIDSIKALANLNVPSSRLII
CHHHHHCCHHEEEEEECCHHHHHHHHHHHHEEEECCEEEEEECCEEEEEECCCCCCEEEE
FLDPPFNKNIVPLALKSILENRHILDQTLIYIETEKTAEYSLEGFDILKEKNSTNISAKL
EECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEE
VSKNHLNSKNNL
EECCCCCCCCCC
>Mature Secondary Structure
MKTNSIRVIAGKYKNRRLKFPNANGLRPTSDQLKETIFNWLSPFIHDSICIDAFAGSGSL
CCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
GIESISRGAAKAIFYELNFKALLQIKENLKTLAIENFEIYKIDSIKALANLNVPSSRLII
CHHHHHCCHHEEEEEECCHHHHHHHHHHHHEEEECCEEEEEECCEEEEEECCCCCCEEEE
FLDPPFNKNIVPLALKSILENRHILDQTLIYIETEKTAEYSLEGFDILKEKNSTNISAKL
EECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEE
VSKNHLNSKNNL
EECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]