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The map label for this gene is rph [H]

Identifier: 134302244

GI number: 134302244

Start: 1250706

End: 1251413

Strand: Reverse

Name: rph [H]

Synonym: FTW_1326

Alternate gene names: 134302244

Gene position: 1251413-1250706 (Counterclockwise)

Preceding gene: 134302249

Following gene: 134302239

Centisome position: 65.92

GC content: 39.12

Gene sequence:

>708_bases
ATGCGTCCAAGTGGTAGAAATAATGATCAACTACGTAATCTAAAAGTTACACATAATTTTACAAAACATGCCGAGGGCTC
TGTCCTTATTGAATTTGGAGATACTAAAGTTATCTGTACAGCTTCTGTAGTTGCAGGTGTACCAAAATTTAAAAAAGATA
GTGGTGAGGGTTGGCTAACAGCAGAATATGGCATGTTGCCGCGTTCAACTCATACGCGTATGGATAGAGAGGCAGCACGT
GGTAAGCAATCTGGAAGAACTCAAGAAATTCAGCGCCTGATAGGTAGAGCATTGCGTGCTAGTGTTGATCTTACAGCTAT
CGGGGAAAATACTATTAAGGTTGATTGTGATGTGATTCAAGCTGATGGTGGTACGCGTACTGCATCGATTACTGGCGCAT
CATTAGCCATAGCTGATGCTATAGAATATATGAAACAAAATGGTATGTTAGATGAGCAGGCAAATCCACTATTATCACAA
GTAGCAGCAATATCTGTAGGTATTTATAATAACGAGCCAGTGCTTGATCTTGATTATGATGAAGATTCAAACGCAGAAAC
TGATATGAATGTAGTGATGAATTCAAATGGCGGTATAATCGAGATTCAAGGCACTGCTGAAGGCAAAGACTTCTCTGAAG
AAGAGTTTGCAAAAATGCTTGGTCTTGCTAAAAAAGGTATCAAAGAGATTTTTGCAACAGTATTCTAG

Upstream 100 bases:

>100_bases
ATATATCAGAAATACCCTAAAGTTAAGGTTTCTTATTTTAATTTCGATTTGTATAATTATACACTTATAAGGCTATGAAA
TTAATTAAAAGGATTCTAAA

Downstream 100 bases:

>100_bases
TCTACAAAATTGCAATCAAAAAACACTTACTTTTATTAATACTAGTTTAAAAGCTACAGGTTATTTATAAAAGAATTAAT
TTTTACAAGTAGTTCAAAAT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRPSGRNNDQLRNLKVTHNFTKHAEGSVLIEFGDTKVICTASVVAGVPKFKKDSGEGWLTAEYGMLPRSTHTRMDREAAR
GKQSGRTQEIQRLIGRALRASVDLTAIGENTIKVDCDVIQADGGTRTASITGASLAIADAIEYMKQNGMLDEQANPLLSQ
VAAISVGIYNNEPVLDLDYDEDSNAETDMNVVMNSNGGIIEIQGTAEGKDFSEEEFAKMLGLAKKGIKEIFATVF

Sequences:

>Translated_235_residues
MRPSGRNNDQLRNLKVTHNFTKHAEGSVLIEFGDTKVICTASVVAGVPKFKKDSGEGWLTAEYGMLPRSTHTRMDREAAR
GKQSGRTQEIQRLIGRALRASVDLTAIGENTIKVDCDVIQADGGTRTASITGASLAIADAIEYMKQNGMLDEQANPLLSQ
VAAISVGIYNNEPVLDLDYDEDSNAETDMNVVMNSNGGIIEIQGTAEGKDFSEEEFAKMLGLAKKGIKEIFATVF
>Mature_235_residues
MRPSGRNNDQLRNLKVTHNFTKHAEGSVLIEFGDTKVICTASVVAGVPKFKKDSGEGWLTAEYGMLPRSTHTRMDREAAR
GKQSGRTQEIQRLIGRALRASVDLTAIGENTIKVDCDVIQADGGTRTASITGASLAIADAIEYMKQNGMLDEQANPLLSQ
VAAISVGIYNNEPVLDLDYDEDSNAETDMNVVMNSNGGIIEIQGTAEGKDFSEEEFAKMLGLAKKGIKEIFATVF

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=213, Percent_Identity=67.1361502347418, Blast_Score=300, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI71981632, Length=194, Percent_Identity=27.8350515463918, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25459; Mature: 25459

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRNNDQLRNLKVTHNFTKHAEGSVLIEFGDTKVICTASVVAGVPKFKKDSGEGWLT
CCCCCCCCCHHEEEEEECCCCCCCCCCEEEEECCCEEEEEHHHHHCCCCCCCCCCCEEEE
AEYGMLPRSTHTRMDREAARGKQSGRTQEIQRLIGRALRASVDLTAIGENTIKVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEE
ADGGTRTASITGASLAIADAIEYMKQNGMLDEQANPLLSQVAAISVGIYNNEPVLDLDYD
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCCEEEECCC
EDSNAETDMNVVMNSNGGIIEIQGTAEGKDFSEEEFAKMLGLAKKGIKEIFATVF
CCCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSGRNNDQLRNLKVTHNFTKHAEGSVLIEFGDTKVICTASVVAGVPKFKKDSGEGWLT
CCCCCCCCCHHEEEEEECCCCCCCCCCEEEEECCCEEEEEHHHHHCCCCCCCCCCCEEEE
AEYGMLPRSTHTRMDREAARGKQSGRTQEIQRLIGRALRASVDLTAIGENTIKVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEE
ADGGTRTASITGASLAIADAIEYMKQNGMLDEQANPLLSQVAAISVGIYNNEPVLDLDYD
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCCEEEECCC
EDSNAETDMNVVMNSNGGIIEIQGTAEGKDFSEEEFAKMLGLAKKGIKEIFATVF
CCCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA