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The map label for this gene is ispG [H]

Identifier: 134302085

GI number: 134302085

Start: 1063573

End: 1064778

Strand: Reverse

Name: ispG [H]

Synonym: FTW_1121

Alternate gene names: 134302085

Gene position: 1064778-1063573 (Counterclockwise)

Preceding gene: 134302086

Following gene: 134302084

Centisome position: 56.09

GC content: 35.99

Gene sequence:

>1206_bases
ATGAATAAAACAAGTGTTGTAAAAGTTGGAAATGTTTTGATAGGTGGTGATAATCCAGTTGTTGTTCAGTCAATGACAGA
TACATATACTGCAGATGTTGAAAAGACAGTTAAGCAAATCTTAGCACTTCATAAAGCAGGTAGTGAAATTGTTAGAATTA
CAGTTAATGATGAGTCAGCTGCTGCTGCTGTACCTGAGATTGTTAAAGAGCTAGCAAAGCATGATTGTCATGTGCCTTTG
GTTGGTGATTTTCATTATAATGGTCATACATTACTTAGTAAATATCCAGAATGTGCGAAGGCTTTGGCAAAATATCGTAT
AAATCCTGGTAATGTTGGTTTTGGTAAGAAAAAAGATACTCAGTTTGCAGAGATTATAAAAATTGCAATAGCAAATGATA
AGCCTGTGCGTATAGGCGTAAACTGGGGAAGCTTAGATCAAGCTTTATTAGCAAGGCTAATTGATGAAAACAATGCTCAA
GAGAATCCACTAAGCTTACAACAAATAATGCATAAGGCACTTATAACTTCAGCTTTAGAAAGTGCTAAATATGCAGAAGA
GCTGGGATTAGCAAAAGATAAAATTATAATCTCATGTAAAGTAAGTGAAGTACAAGATTTAATTGCAGTATATCAAAAAC
TAGCTAAAGAGTGTGATTATGCTTTACACCTAGGATTAACAGAAGCTGGTATGGGTACTAAAGGTATTGTTGCTAGTGCT
GTGAGTTTAGGAATTTTATTACAGCAAGGTATAGGTAACACTATCAGAGTTTCATTAACTCCAGCGCCAAATGCTCCACG
TACAGAAGAGGTGCGTGTATGTCGTGAAATCCTTCAAAATCTAGGCATGAGAACATTTACTCCAAGTGTAACATCTTGTC
CAGGTTGTGGTAGGACTACTAGCTCGTTTTTTAGAGAACTAACAAGCAAAGTTAAAGATCATTTGGATGAAAAAATGCAT
ACTTGGAAAGAGCAATATCACGGCGTAGAAGCAATGAAAGTGGCTGTAATGGGCTGTGTTGTTAATGGTCCAGGCGAATC
AAAAAATGCTGATATTGGTATAAGCCTACCAGGTAGTGGCGAGTCGCCTGTTGCACCAGTTTTCATCGATGGTAAAAAAG
CACATACTCTAAGAGGGGATAACATTTCTGAAGAATTTATAGAGATTGTAGAAAATTATGTTAAAAATCGTTATGGTAAA
AAATAG

Upstream 100 bases:

>100_bases
ACATAGCTTATGAAAATTAATCTTTATAATGATATAATCTTACATTGTATACTTTTCGAAGAATTATTTAAAAAATAAAT
TATGATTTTTGGGTTCTTAT

Downstream 100 bases:

>100_bases
GTAAATACTAGAAAATGTCAGTAGTTTTTGTTAATACCCCCTTTGCCAAACTTAAATTAGGTTATACAAATAATACCCAA
GGCTTTAGTAAAGAAAAATA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNKTSVVKVGNVLIGGDNPVVVQSMTDTYTADVEKTVKQILALHKAGSEIVRITVNDESAAAAVPEIVKELAKHDCHVPL
VGDFHYNGHTLLSKYPECAKALAKYRINPGNVGFGKKKDTQFAEIIKIAIANDKPVRIGVNWGSLDQALLARLIDENNAQ
ENPLSLQQIMHKALITSALESAKYAEELGLAKDKIIISCKVSEVQDLIAVYQKLAKECDYALHLGLTEAGMGTKGIVASA
VSLGILLQQGIGNTIRVSLTPAPNAPRTEEVRVCREILQNLGMRTFTPSVTSCPGCGRTTSSFFRELTSKVKDHLDEKMH
TWKEQYHGVEAMKVAVMGCVVNGPGESKNADIGISLPGSGESPVAPVFIDGKKAHTLRGDNISEEFIEIVENYVKNRYGK
K

Sequences:

>Translated_401_residues
MNKTSVVKVGNVLIGGDNPVVVQSMTDTYTADVEKTVKQILALHKAGSEIVRITVNDESAAAAVPEIVKELAKHDCHVPL
VGDFHYNGHTLLSKYPECAKALAKYRINPGNVGFGKKKDTQFAEIIKIAIANDKPVRIGVNWGSLDQALLARLIDENNAQ
ENPLSLQQIMHKALITSALESAKYAEELGLAKDKIIISCKVSEVQDLIAVYQKLAKECDYALHLGLTEAGMGTKGIVASA
VSLGILLQQGIGNTIRVSLTPAPNAPRTEEVRVCREILQNLGMRTFTPSVTSCPGCGRTTSSFFRELTSKVKDHLDEKMH
TWKEQYHGVEAMKVAVMGCVVNGPGESKNADIGISLPGSGESPVAPVFIDGKKAHTLRGDNISEEFIEIVENYVKNRYGK
K
>Mature_401_residues
MNKTSVVKVGNVLIGGDNPVVVQSMTDTYTADVEKTVKQILALHKAGSEIVRITVNDESAAAAVPEIVKELAKHDCHVPL
VGDFHYNGHTLLSKYPECAKALAKYRINPGNVGFGKKKDTQFAEIIKIAIANDKPVRIGVNWGSLDQALLARLIDENNAQ
ENPLSLQQIMHKALITSALESAKYAEELGLAKDKIIISCKVSEVQDLIAVYQKLAKECDYALHLGLTEAGMGTKGIVASA
VSLGILLQQGIGNTIRVSLTPAPNAPRTEEVRVCREILQNLGMRTFTPSVTSCPGCGRTTSSFFRELTSKVKDHLDEKMH
TWKEQYHGVEAMKVAVMGCVVNGPGESKNADIGISLPGSGESPVAPVFIDGKKAHTLRGDNISEEFIEIVENYVKNRYGK
K

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=384, Percent_Identity=33.8541666666667, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 43472; Mature: 43472

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTSVVKVGNVLIGGDNPVVVQSMTDTYTADVEKTVKQILALHKAGSEIVRITVNDESA
CCCCCEEEECCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHH
AAAVPEIVKELAKHDCHVPLVGDFHYNGHTLLSKYPECAKALAKYRINPGNVGFGKKKDT
HHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCH
QFAEIIKIAIANDKPVRIGVNWGSLDQALLARLIDENNAQENPLSLQQIMHKALITSALE
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SAKYAEELGLAKDKIIISCKVSEVQDLIAVYQKLAKECDYALHLGLTEAGMGTKGIVASA
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCCHHCCCCCHHHHHHH
VSLGILLQQGIGNTIRVSLTPAPNAPRTEEVRVCREILQNLGMRTFTPSVTSCPGCGRTT
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCH
SSFFRELTSKVKDHLDEKMHTWKEQYHGVEAMKVAVMGCVVNGPGESKNADIGISLPGSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCCCCCEEEECCCCC
ESPVAPVFIDGKKAHTLRGDNISEEFIEIVENYVKNRYGKK
CCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKTSVVKVGNVLIGGDNPVVVQSMTDTYTADVEKTVKQILALHKAGSEIVRITVNDESA
CCCCCEEEECCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHH
AAAVPEIVKELAKHDCHVPLVGDFHYNGHTLLSKYPECAKALAKYRINPGNVGFGKKKDT
HHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCH
QFAEIIKIAIANDKPVRIGVNWGSLDQALLARLIDENNAQENPLSLQQIMHKALITSALE
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SAKYAEELGLAKDKIIISCKVSEVQDLIAVYQKLAKECDYALHLGLTEAGMGTKGIVASA
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCCHHCCCCCHHHHHHH
VSLGILLQQGIGNTIRVSLTPAPNAPRTEEVRVCREILQNLGMRTFTPSVTSCPGCGRTT
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCH
SSFFRELTSKVKDHLDEKMHTWKEQYHGVEAMKVAVMGCVVNGPGESKNADIGISLPGSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCCCCCEEEECCCCC
ESPVAPVFIDGKKAHTLRGDNISEEFIEIVENYVKNRYGKK
CCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA