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The map label for this gene is mdh [H]

Identifier: 134301988

GI number: 134301988

Start: 956489

End: 957448

Strand: Direct

Name: mdh [H]

Synonym: FTW_1007

Alternate gene names: 134301988

Gene position: 956489-957448 (Clockwise)

Preceding gene: 134301985

Following gene: 134301989

Centisome position: 50.38

GC content: 36.15

Gene sequence:

>960_bases
ATGGCTAGAAAAAAAATAACACTTGTTGGTGCTGGTAATATTGGTGGTACACTAGCTCACTTAGCACTTATAAAACAATT
AGGTGATGTAGTCCTTTTTGATATAGCTCAGGGCATGCCTAATGGCAAAGCACTTGATTTACTACAAACTTGCCCTATCG
AGGGAGTTGATTTTAAAGTCAGGGGTACAAATGATTACAAAGATTTAGAAAATTCTGATGTTGTTATTGTAACTGCTGGT
GTTCCGAGAAAGCCTGGCATGTCTCGTGATGACCTACTTGGTATTAATATCAAAGTAATGCAAACAGTCGGAGAAGGTAT
CAAACATAACTGTCCTAATGCTTTCGTAATATGTATCACAAATCCACTAGATATTATGGTAAATATGTTACAAAAATTCT
CAGGCGTACCAGATAACAAAATCGTTGGTATGGCTGGTGTTTTAGATTCTGCTAGATTTAGAACATTTTTAGCAGATGAA
CTAAATGTATCAGTACAACAAGTACAAGCATATGTAATGGGCGGTCATGGCGATACTATGGTCCCTCTAACTAAAATGTC
AAATGTTGCAGGTGTGTCGTTAGAGCAGCTAGTTAAAGAAGGTAAACTTAAACAAGAGCGTTTAGATGCTATAGTATCGA
GAACTAGAAGTGGTGGTGGCGAAATTGTTGCTTTACTTAAAACAGGTTCAGCATATTATGCACCAGCTGCTGCAGGTATT
CAAATGGCTGAAAGCTTCTTAAAAGATAAAAAGATGATTTTACCTTGTGCTGCAAAAGTAAAAGCTGGTATGTATGGCCT
TGATGAAGATCTATTTGTTGGTGTACCAACTGAGATTTCTGCAAATGGTGTCAGACCTATTGAAGTAGAGATTTCTGATA
AAGAAAGAGAACAATTACAAGTATCTATAAACGCTATAAAAGATCTTAATAAAGCAGCTGCTGAAATTTTAGCAAAATAA

Upstream 100 bases:

>100_bases
TAAAGCTTACAAAACTTTTCATTGAATAACGACTAAACTATTAGTTATAATGGTTAGTATCTACTTTGATTCAGTTTTTA
GCACAATAAAGGAGATTTTA

Downstream 100 bases:

>100_bases
TTTTTTAAGATATTTTAGACAGTAAGCATTGTACTGTCTACATCTTATAACTGACTCACCAATGTCAAGTACAATATTTG
ACTTTTTAGTATTCTAAAAT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MARKKITLVGAGNIGGTLAHLALIKQLGDVVLFDIAQGMPNGKALDLLQTCPIEGVDFKVRGTNDYKDLENSDVVIVTAG
VPRKPGMSRDDLLGINIKVMQTVGEGIKHNCPNAFVICITNPLDIMVNMLQKFSGVPDNKIVGMAGVLDSARFRTFLADE
LNVSVQQVQAYVMGGHGDTMVPLTKMSNVAGVSLEQLVKEGKLKQERLDAIVSRTRSGGGEIVALLKTGSAYYAPAAAGI
QMAESFLKDKKMILPCAAKVKAGMYGLDEDLFVGVPTEISANGVRPIEVEISDKEREQLQVSINAIKDLNKAAAEILAK

Sequences:

>Translated_319_residues
MARKKITLVGAGNIGGTLAHLALIKQLGDVVLFDIAQGMPNGKALDLLQTCPIEGVDFKVRGTNDYKDLENSDVVIVTAG
VPRKPGMSRDDLLGINIKVMQTVGEGIKHNCPNAFVICITNPLDIMVNMLQKFSGVPDNKIVGMAGVLDSARFRTFLADE
LNVSVQQVQAYVMGGHGDTMVPLTKMSNVAGVSLEQLVKEGKLKQERLDAIVSRTRSGGGEIVALLKTGSAYYAPAAAGI
QMAESFLKDKKMILPCAAKVKAGMYGLDEDLFVGVPTEISANGVRPIEVEISDKEREQLQVSINAIKDLNKAAAEILAK
>Mature_318_residues
ARKKITLVGAGNIGGTLAHLALIKQLGDVVLFDIAQGMPNGKALDLLQTCPIEGVDFKVRGTNDYKDLENSDVVIVTAGV
PRKPGMSRDDLLGINIKVMQTVGEGIKHNCPNAFVICITNPLDIMVNMLQKFSGVPDNKIVGMAGVLDSARFRTFLADEL
NVSVQQVQAYVMGGHGDTMVPLTKMSNVAGVSLEQLVKEGKLKQERLDAIVSRTRSGGGEIVALLKTGSAYYAPAAAGIQ
MAESFLKDKKMILPCAAKVKAGMYGLDEDLFVGVPTEISANGVRPIEVEISDKEREQLQVSINAIKDLNKAAAEILAK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=31.4285714285714, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=31.4285714285714, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=31.3099041533546, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=31.3099041533546, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI291575128, Length=317, Percent_Identity=32.1766561514196, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4557032, Length=317, Percent_Identity=32.1766561514196, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI5031857, Length=313, Percent_Identity=30.6709265175719, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI260099723, Length=313, Percent_Identity=30.6709265175719, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI15082234, Length=311, Percent_Identity=27.9742765273312, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=35.2040816326531, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=35.2040816326531, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI207028494, Length=196, Percent_Identity=30.1020408163265, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI21735621, Length=255, Percent_Identity=32.5490196078431, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI103472011, Length=312, Percent_Identity=23.3974358974359, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1789632, Length=294, Percent_Identity=33.6734693877551, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=286, Percent_Identity=24.4755244755245, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17554310, Length=294, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=301, Percent_Identity=30.2325581395349, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324446, Length=352, Percent_Identity=29.2613636363636, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=214, Percent_Identity=31.7757009345794, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136226, Length=315, Percent_Identity=28.8888888888889, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=27.8688524590164, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647881, Length=326, Percent_Identity=33.4355828220859, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24663599, Length=318, Percent_Identity=30.5031446540881, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24663595, Length=258, Percent_Identity=30.2325581395349, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34091; Mature: 33960

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKITLVGAGNIGGTLAHLALIKQLGDVVLFDIAQGMPNGKALDLLQTCPIEGVDFKV
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEHHHHCCCCCCHHHHHHHHCCCCCCEEEE
RGTNDYKDLENSDVVIVTAGVPRKPGMSRDDLLGINIKVMQTVGEGIKHNCPNAFVICIT
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEHHHHHHHHHCCCCCEEEEEEC
NPLDIMVNMLQKFSGVPDNKIVGMAGVLDSARFRTFLADELNVSVQQVQAYVMGGHGDTM
CHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCE
VPLTKMSNVAGVSLEQLVKEGKLKQERLDAIVSRTRSGGGEIVALLKTGSAYYAPAAAGI
EEHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCHHHHH
QMAESFLKDKKMILPCAAKVKAGMYGLDEDLFVGVPTEISANGVRPIEVEISDKEREQLQ
HHHHHHHHCCCEEECCHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCHHHHH
VSINAIKDLNKAAAEILAK
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARKKITLVGAGNIGGTLAHLALIKQLGDVVLFDIAQGMPNGKALDLLQTCPIEGVDFKV
CCEEEEEEECCCCCHHHHHHHHHHHHHHHEEHHHHCCCCCCHHHHHHHHCCCCCCEEEE
RGTNDYKDLENSDVVIVTAGVPRKPGMSRDDLLGINIKVMQTVGEGIKHNCPNAFVICIT
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEHHHHHHHHHCCCCCEEEEEEC
NPLDIMVNMLQKFSGVPDNKIVGMAGVLDSARFRTFLADELNVSVQQVQAYVMGGHGDTM
CHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCE
VPLTKMSNVAGVSLEQLVKEGKLKQERLDAIVSRTRSGGGEIVALLKTGSAYYAPAAAGI
EEHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCHHHHH
QMAESFLKDKKMILPCAAKVKAGMYGLDEDLFVGVPTEISANGVRPIEVEISDKEREQLQ
HHHHHHHHCCCEEECCHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCHHHHH
VSINAIKDLNKAAAEILAK
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA