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The map label for this gene is dacB1 [H]

Identifier: 134301937

GI number: 134301937

Start: 912601

End: 914004

Strand: Direct

Name: dacB1 [H]

Synonym: FTW_0948

Alternate gene names: 134301937

Gene position: 912601-914004 (Clockwise)

Preceding gene: 134301931

Following gene: 134301938

Centisome position: 48.07

GC content: 32.19

Gene sequence:

>1404_bases
TTGAAAAGAAAAACTAAGTTTTTTACGGCATTCTCTATATTACTTATAAGCATAGCAACTACTCAAGCATCTACGCGTAG
TGAGGTTCAATATCTTGTAAAAAAATATAATCTCTCTGATGCTAAAATTGCTATAGCAACTCAAACAACTAATAATGGTG
ATCAATTATATGCCTATGCCCAAAATAGATTAATGACGCCAGCAAGTACTAATAAGGTTTTTACAATAGTTGCGGCACTT
TTTACGATACCTAGTAATTTTAGATTTACAACATCAATAATGTACCCTTCCGATAGAGTAAAAGATCATACTCTGTATGG
AGATATGTACATAAAATTTACTGGTGATCCAGCACTAACTGGTAGTCAGTTAGCCACATTAATAAAAAAAATTAAAACTG
AGAAAGATATATCTAAAATAACTGGTGATGTTTACCTTGTTGGAGTTTTCTCTGGGCCTTATATCCCAAATGGTTGGTCT
AAAGAAGATAGTACATTCTGTTTTGGTGCTCCAGCATCAAGCTTCACACTTAATAGAAATTGTACAGTCATAAAATTAGT
AAAAAATACTAATAGTTTAACAACAAGAATTGTTGAATTAAGTAACGCAAGTAATATCACTATAAAAAATACCGCAAAAT
ATACTAGTGCATCAAGTGCAACTACGATAGAAATGAATAATGATAATGTTTTATATATTGGTGGATACTTATCAAGAGCT
GCTGAAAAAATGTTTAAATTAGCAATTAAAAATCCCGCGCTTAAAACATTAGATACCGTAAATGACTTCTTAAATTCTGA
CGGTATTAAACATGGTAACGTTATTATAGCTGGTAGTGTTCCTACTGGTTATACTGAACAGATTACAACAAGGTCTCAAA
CAATCGGACATTTTATTGATCAAGCCCTTAAACATTCTAATAATCTATATGCAGAGACTATTTTAAATACTTTAGGCTTA
AAAGAAAAAGGTATTGGCTCAACTAAAGCAGGTACGGAAGCTGTTCAAAGTATACTCTACTCAAAACTAGGCTTGGATAC
ATCAGCTTTAACAATGTATGACGGTTCAGGTCTATCACATCTAGATAAAGTAACTCCAGAATTTATGGTCAACTTTTTGA
CCAAAGCTTATAATAGTCAGATTGGTAAAGAATTCTATAACTACTTATCAGCATCTGGTATAAGCGGTACAATTTCATAT
AGAATGGGTGGTAAACTACTTGGACGAGTACATGCTAAAACTGGTACTCTATCCGGAGTATCAACGTTATCAGGCTATTT
ATTAACAGCTAAAAACCACAGAATCAGTTTTTCGATTATGCTTAATGATCTTAAACCCTCTGATCGTTATAATGCACGAA
GATTCCAAGATAAACTAGTTGATGTCTTCTATAGGAATTTATAA

Upstream 100 bases:

>100_bases
TCTAAGATTAGATAATATACTATAACTTACTGTATTTATAAGAGATAAAATGCTATTATCTCGGTTAAGGATTCTTTAGA
AATTTATATGGTTAAAGGTT

Downstream 100 bases:

>100_bases
ATGAAAAAAATAATTAAGCTTGCAACTTTAATTATCATAATAACAACTCTTGGATCTTGCGCTACTGAGCCTCCAACAAA
TATAAATAATGCTTGTAGCA

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKRKTKFFTAFSILLISIATTQASTRSEVQYLVKKYNLSDAKIAIATQTTNNGDQLYAYAQNRLMTPASTNKVFTIVAAL
FTIPSNFRFTTSIMYPSDRVKDHTLYGDMYIKFTGDPALTGSQLATLIKKIKTEKDISKITGDVYLVGVFSGPYIPNGWS
KEDSTFCFGAPASSFTLNRNCTVIKLVKNTNSLTTRIVELSNASNITIKNTAKYTSASSATTIEMNNDNVLYIGGYLSRA
AEKMFKLAIKNPALKTLDTVNDFLNSDGIKHGNVIIAGSVPTGYTEQITTRSQTIGHFIDQALKHSNNLYAETILNTLGL
KEKGIGSTKAGTEAVQSILYSKLGLDTSALTMYDGSGLSHLDKVTPEFMVNFLTKAYNSQIGKEFYNYLSASGISGTISY
RMGGKLLGRVHAKTGTLSGVSTLSGYLLTAKNHRISFSIMLNDLKPSDRYNARRFQDKLVDVFYRNL

Sequences:

>Translated_467_residues
MKRKTKFFTAFSILLISIATTQASTRSEVQYLVKKYNLSDAKIAIATQTTNNGDQLYAYAQNRLMTPASTNKVFTIVAAL
FTIPSNFRFTTSIMYPSDRVKDHTLYGDMYIKFTGDPALTGSQLATLIKKIKTEKDISKITGDVYLVGVFSGPYIPNGWS
KEDSTFCFGAPASSFTLNRNCTVIKLVKNTNSLTTRIVELSNASNITIKNTAKYTSASSATTIEMNNDNVLYIGGYLSRA
AEKMFKLAIKNPALKTLDTVNDFLNSDGIKHGNVIIAGSVPTGYTEQITTRSQTIGHFIDQALKHSNNLYAETILNTLGL
KEKGIGSTKAGTEAVQSILYSKLGLDTSALTMYDGSGLSHLDKVTPEFMVNFLTKAYNSQIGKEFYNYLSASGISGTISY
RMGGKLLGRVHAKTGTLSGVSTLSGYLLTAKNHRISFSIMLNDLKPSDRYNARRFQDKLVDVFYRNL
>Mature_467_residues
MKRKTKFFTAFSILLISIATTQASTRSEVQYLVKKYNLSDAKIAIATQTTNNGDQLYAYAQNRLMTPASTNKVFTIVAAL
FTIPSNFRFTTSIMYPSDRVKDHTLYGDMYIKFTGDPALTGSQLATLIKKIKTEKDISKITGDVYLVGVFSGPYIPNGWS
KEDSTFCFGAPASSFTLNRNCTVIKLVKNTNSLTTRIVELSNASNITIKNTAKYTSASSATTIEMNNDNVLYIGGYLSRA
AEKMFKLAIKNPALKTLDTVNDFLNSDGIKHGNVIIAGSVPTGYTEQITTRSQTIGHFIDQALKHSNNLYAETILNTLGL
KEKGIGSTKAGTEAVQSILYSKLGLDTSALTMYDGSGLSHLDKVTPEFMVNFLTKAYNSQIGKEFYNYLSASGISGTISY
RMGGKLLGRVHAKTGTLSGVSTLSGYLLTAKNHRISFSIMLNDLKPSDRYNARRFQDKLVDVFYRNL

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=447, Percent_Identity=27.2930648769575, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51341; Mature: 51341

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKTKFFTAFSILLISIATTQASTRSEVQYLVKKYNLSDAKIAIATQTTNNGDQLYAYA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEE
QNRLMTPASTNKVFTIVAALFTIPSNFRFTTSIMYPSDRVKDHTLYGDMYIKFTGDPALT
CCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCC
GSQLATLIKKIKTEKDISKITGDVYLVGVFSGPYIPNGWSKEDSTFCFGAPASSFTLNRN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEECCC
CTVIKLVKNTNSLTTRIVELSNASNITIKNTAKYTSASSATTIEMNNDNVLYIGGYLSRA
CEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCCEEEECHHHHHH
AEKMFKLAIKNPALKTLDTVNDFLNSDGIKHGNVIIAGSVPTGYTEQITTRSQTIGHFID
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QALKHSNNLYAETILNTLGLKEKGIGSTKAGTEAVQSILYSKLGLDTSALTMYDGSGLSH
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHH
LDKVTPEFMVNFLTKAYNSQIGKEFYNYLSASGISGTISYRMGGKLLGRVHAKTGTLSGV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHEEECCCCCCCCH
STLSGYLLTAKNHRISFSIMLNDLKPSDRYNARRFQDKLVDVFYRNL
HHHCCEEEEECCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRKTKFFTAFSILLISIATTQASTRSEVQYLVKKYNLSDAKIAIATQTTNNGDQLYAYA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEE
QNRLMTPASTNKVFTIVAALFTIPSNFRFTTSIMYPSDRVKDHTLYGDMYIKFTGDPALT
CCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCCC
GSQLATLIKKIKTEKDISKITGDVYLVGVFSGPYIPNGWSKEDSTFCFGAPASSFTLNRN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEECCC
CTVIKLVKNTNSLTTRIVELSNASNITIKNTAKYTSASSATTIEMNNDNVLYIGGYLSRA
CEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCCEEEECHHHHHH
AEKMFKLAIKNPALKTLDTVNDFLNSDGIKHGNVIIAGSVPTGYTEQITTRSQTIGHFID
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QALKHSNNLYAETILNTLGLKEKGIGSTKAGTEAVQSILYSKLGLDTSALTMYDGSGLSH
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHH
LDKVTPEFMVNFLTKAYNSQIGKEFYNYLSASGISGTISYRMGGKLLGRVHAKTGTLSGV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHEEECCCCCCCCH
STLSGYLLTAKNHRISFSIMLNDLKPSDRYNARRFQDKLVDVFYRNL
HHHCCEEEEECCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]